Purification and Characterization of a Xylanase from Bacillus sp . KK-1

Dae Joon Kim,Ki Hong Yoon Seung Ho Kim,Ki Haeng Cho,Bon Hong Min 생화학분자생물학회 1995 BMB Reports Vol.28 No.4

A thermophilic Bacillus sp. strain KK-1 isolated from soil produced an extracelluar xylanase. From the culture supernatant of Bacillus sp., the xylanase was purified to homogeneity by ammonium sulfate precipitation and DEAF-Sephadex A-50 chromatography. The molecular weight of the purified xylanase was estimated to be 45 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel permeation chromatography. The apparent K_m values for xylanase, using oat spelt xylan and birchwood xylan as substrates, were 7.1 ㎎/㎖ and 3.2 ㎎/㎖, and V_(max) values were 27.0 μ㏖·min^(-1)·㎎^(-1) and 29.0 μ㏖·min^(-1)·㎎^(-1), respectively. The xylanase hydrolyzed oat spelt xylan to mostly xylobiose, xytotriose, and xylose. The amino acid composition indicated that the xylanase contained high amounts of amino acid residues of glutamic acid and glutamine (Glx) and aspartic acid and asparagine (Asx).

정읍지역의 다양한 지표변위 인자들을 이용한 연약지반 리스크 평가

이효진(Lee, Hyo Jin),윤홍식(Yoon, Hong Sic),염민교(Yeom, Min Kyo),이준민(Lee, Joon Min) 한국측량학회 2018 한국측량학회 학술대회자료집 Vol.2018 No.4

연약지반의 지반침하는 고속철도의 열차 탈선, 심하게는 건물의 붕괴 등 대형재난을 야기할 우려가 있다. 그렇기 때문에 지반침하를 사전에 파악해 재난을 예방하기 위한 다양한 연구가 진행 중이다. 최근 광범위한 지역의 지표변위를 분석할 수 있는 InSAR(interferometric SAR)기법을 이용한 연구가 활발히 진행되고 있다. InSAR 기법 중 PS-InSAR(Permanent Scatterer Interferometric SAR)기법이란 SAR영상에서 긴밀도가 높은 고정산란체(PS:Permanent scatterer)를 이용하여 지반침하를 분석하는 기법으로 다른 InSAR 기법에 비해 대기에 의해 발생하는 오차가 적으며, 보다 정확한 지표 변위값을 도출할 수 있다. 또한 장기간에 걸친 시계열적 지표 변위 분석에 용이하다. 본 논문에서는 독일의 Terra SAR-X 위성 영상을 이용하여, 정읍지역의 지표변위를 분석하였다. 지반침하 데이터를 누적 지표 변위. MIN-MAX값, 그리고 최종침하량을 종합적으로 분석하여 보다 정확하게 결과를 도출하고자 하였다. 연구지역인 정읍시는 평야지역과 산지의 구분이 뚜렷하게 나타나는 지역이며, 연약지반인 평 야지역의 지반침하를 발견할 수 있었다. 연구 결과 정읍 신태인 지역은 최대 -11mm/year, 평균-3mm/year로 지반이 침하되고 있음을 확인할 수 있었다. 정읍지역의 지반침하 대비를 위해 다양한 노력들이 필요할 것으로 사료된다.

감조하천의 유로단면변화에 따른 유황해석

민병형,민일규,이승휘,박한기,박홍준 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

The subject of this study apply to KyungSangNamDo Sachun-Si Sachun-bay sea area for builds up a industrial complex, and inquire into the .phenomenon in compared after with before reclamation. I investigated, how to remove these harmful influence and make a estimate that a water-level, flow direction and river-bed evolution by inflow of river, wave's attack. So we have come to the conclusion as follow. 1. Water Level After reclamation, the water-level is ascended, but the water-level is stable after dredging and changing water-course. 2. Velocity of Flow : After reclamation, back water broke out, the velocity of flow was faster after dredging and changing water-course, but the velocity of flow came to be stable in the downstream. 3. River-bed Evolution : After reclamation, the severe erosion occured in the front of revetment, but after d]:edging and changing the water-course as the new stable water-course constituted, river bed evolution decreased and the flood wave flow smoothly. 4. After reclamation, the water-level remarkablely increased, but the velocity of flow was nearly the same.

SMS를 이용한 경안천 하류구간의 하천흐름 분석

홍성민,정인균,이준우,김성준 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.1

본 연구는 경안천 하류구간(13.8㎞)을 대상으로 SMS의 RMA2 모형과 SES2D 모형을 이용하여 2002년 경안관측소의 최대홍수위를 기록한 8월 7일의 수리분석을 실시하였다. RMA2 모형을 적용하기 위하여 RIMGIS 데이터의 하천측량자료를 이용하여 입력자료로 활용하였으며, 생성돈 지형자료를 2차원 유한요소망으로 변환하여 모형을 수행하였다. 본 연구에서는 부정류 상태의 모의를 적용하였으며, 모형에 민감하게 반응하는 인자에 대해서는 선행 연구에서 실시한 민감도 분석결과를 고려하였고, 기타 입력인자는 통계연보 및 하천정비계획서를 참고하였다. 또한 대상하천에 SED2D 모형을 적용하여 홍수사상에 대한 하천의 하상 변동에 대한 모의를 실시하였으며, 대상 하천의 홍수시 유속은 0.05~3.85㎧의 분포로 상류와 하류의 큰 차이를 보였고, 하상변화는 0.0003~0.0135m가 발생하였다. The purpose of this study is to analyze various hydraulic characteristics using SMS(Surface Water Modeling System) RMA2 model. It is based on 2-D finite element method, River reaches (13.8㎞) from Gyeongan gauge station to the inlet of Paldang lake was selected. Finite element was made by RINGIS Data, and the analysis of river-changes was operated by unsteady flow. The sediment concentration and bed change was simulated using SED2D model. This River's velocity was distributed that 0.05~3.85㎧ and bed change was changed about 0.0003~0.0135m.

감조하천의 유로단면변화에 따른 유황해석

민병형,민일규,이승휘,박한기,박홍준 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

The subject of this study apply to KyungSangNamDo Sachun-Si Sachun-bay sea area for builds up a industrial complex, and inquire into the phenomenon in compared after with before reclamation. I investigated, how to remove these harmful influence and make a estimate that a water-level, flow direction and river-bed evolution by inflow of river, wave' s attack. So we have come to the conclusion as follow. 1. Water Level : After reclamation, the water-level is ascended, but the water-level is stable after dredging and changing water-course. 2. Velocity of Flow : After reclamation, back water broke out, the velocity of flow was faster after dredging and changing water-course, but the velocity of flow came to be stable in the downstream. 3. River-bed Evolution : After reclamation, the severe erosion occurred in the front of revetment, but after dredging and changing the water-course as the new stable water-course constituted, river bed evolution decreased and the flood wave flow smoothly. 4. After reclamation, the water-level remarkably increased, but the velocity of flow was nearly the same.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Glucose Oxidase가 척수지각신경절세포에 미치는 영향과 천마의 효과에 관한 연구

송호준,이용석,손영우,이강창,정종길,신민교,홍기연 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : To clarify the cytotoxic effect of glucose oxidase(GO) and protective effect of gastrodiae Rhizoma (GR) on spinal sensory ganglion(DRG) neurons, neurotoxicity mediated by GO was measured by MTT assay and neurofilament enzymeimmuno assay(EIA). Methods : DRG neurons were cultured in the media containing various concentrations of GO for 3 hours. In addition, neuroprotective effects of GR, on GO-induced neurotoxicity in DRG neurons were examined after DRG neurons were preincubated with various concentrations of GR for 2 hours before 15mU/ml GO for 3 hours. Results : GO decreased remarkably cell viability in dependently in these cultures, and also RG increased cell viability and amount of neurofilament in DRG neurons damaged by GO. Conclusion : It is suggested that GO has toxic effect in cultured mouse DRG neurons, and also RG was effective in the protection of GO-induced neurotoxicity in these cultures.

韓國産 蓼(여뀌)科 植物에 관한 本草學的 硏究 (I)

柳泓善,宋昊埈,辛民敎,金裕鎭,金台晛 한국전통의학연구소 1998 한국전통의학지 Vol.8 No.2

For the purpose of developing Korean Herbalogy with the plants of Polygonaceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigative and the results obtained were as follows: 1. There were totaled to 16 genera and 156 species in Korea and among them medicinal plants are 12 genera, 47 species, some 30% in total, but the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Polygonaceae family were classified as Herba 21, Radix 12, Foilum 8, Lignum 5, Flos 4, Fructus 5, Ramulus 2 and Semen 1. Thus it was noticed that Herba was the main kind. 3. According to sum of 156 species in Polygonaceae family, they were classified into Persicaria genera 63, Polygonum genera 23, Rumex genera 22, Bistorta genera 14, Aconogonum genera 6, Fagopyrum genera 5, Bilderdykia genera 4, Amblygonum genera 3, Reynoutria genera 3, Rheum genera 3, Pleuropterus genera 2, Pleuropteropyrum genera 2, Tovara genera 2, Ampelygonum genera 1, Homalocladium genera 1 and Oxyria genera 1. Thus it was noticed that Persicaria genera was the main kind, some 40.3% in total. 4. According to nature and flavour of medicinal plants, they were classified into cold, cool; 23 each, warmth, heat; 11 each, balance 11 and unidentified 5. Thus it was noticed that cold, cool is the main in nature and flavour of medicinal plants. From this result, it was revealed that the plants for medical purpose in Polygonaceae was 30% of the whole, in which HERBA was mostly abundunt and toxic plants was slightly poisonous that it will be used for clinical treatments more easily. It is considered that many experiements and clinical approaches must be continued to use Polygonaceae plants widely.

特性 부가 文法에 依한 類似 標的의 區分

김항준,홍순목,박민호 경북대학교 공과대학 1983 工大硏究誌 Vol.12 No.-

syntactic method is a general tool for pattern recognition but it's not good at distinguishing similar object. In this paper, we use attributed grammar to distinguish long nose plier from diagonal cutter, and good experimental results are obtained.

하수 2차 처리수 재이용을 위한 저압 및 중압 고도산화시스템의 성능평가

안규홍,안석,맹승규,김기팔,홍준석,정민우,권지향,Ahmed, Zubair 대한상하수도학회 2003 상하수도학회지 Vol.17 No.4

In the present study, the feasibility of UV/H₂O₂ systems was investigated using low and medium-pres sure lamps on biologically treated wastewater effluents for secondary effluent reclamation. Two types of UV lamps were used as the light sources (a 33W lowpressure mercury lamp and a 350-W mediumpressure mercury lamp). The results from these UV systems showed that the removal of organic compounds could be achieved in the contact time of longer than 30min (i.e., low UV doses). Efficiencies of color removal and disinfection were far better than those of organic matters measured as TOC, DOC and TCOD_(cr). In the low pressure lamp UV system, it has been found that DOC and color removals were 60.9 and 86.2% with 50㎎/L of H₂O₂ and contact times of 30 minute, respectively. Whereas, with the mediumpressure lamp UV system, TOC, DOC and color removal were 27.1, 5.6 and 95% with 14.3㎎/L of H₂O₂ and 14 minute of contact times, respectively. Both systems could be applied for the reclamation of secondary effluent treated with biological treatment processes.