[논문]Operational Characteristics of CO2 Laser Adopted by Superposing-Discharge

Hee-Je Kim,Hyun-Ju Chung,Byoung-Dae Min,Jong-Han Joung,Sung- lun Park 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

오늘날 현저한 CO2 레이저의 응용 추세에 발맞추어 보다 다양한 형태의 출력 제어 기술이 대두되고 있다. 본연구에서는multi-Pulse Superposing-Discharge 기술을 이용하여 다양한형태의 CO2 레이저의 펄스를 성형 하였다. 펄스 성형을 위한 방법으로 PIC one-chip microprocessor를 이용하여 삼중 PFN 모률의 SCR을 각각 고유의 지연시간을 가지도록 제어하였다. 실험에 사용된 삼중 PFN 모률의 전원 회로는 각각 캐패시터 인덕터,SCR,고압 펄스 트랜스 그리고 고압 트랜스 2차측에 브릿지 정류기로 구성 하였다. 고압 펼스 트랜스의 1차측을 제어하므로서 PFN 모률의 동작을 저 전압에서 수행 할 수 있다. 트랜스의 2차측에 연결된 전파 정류기는 펄스 에너지를 부하에 지속적으로 전달한다. 본 연구에서는 삼중 PFN 모률의 각각의 SCR을 다른 시간 가격을 두고 트리거 하므로서 다양한 Long pulse shaping을 실현하였다. 또한 약 250-1000μs 의 주기를 가지는 다양한 펄스형 레이저 빔을 얻었다.

有限要素法을 이용한 過壓密粘土의 壓密沈下豫測

현상락,류제천,김송만,박춘수 충남대학교 산업기술연구소 2002 산업기술연구논문집 Vol.17 No.1

Consolidation is the time-dependent settlement of soils resulting from the expulsion of water from the soil pores. When a saturated compressible clay layer is subjected to a stress increase, consolidation settlement occurs greatly. Because the hydraulic conductivity of clay is significantly small, the excess pore water pressure generated by loading gradually dissipates over a long period. The settlement caused by consolidation in clay may be several times greater than the immediate settlement. A number of researches are carried out to predict the consolidation behavior in field, however, most of researches show the discrepancies between the prediction and the field settlement data. Therefore, the estimation of consolidation rate is one of the important factors in the construcion on soft clayey deposits. In this paper, laboratory tests have analyzed to apply accurately the soil parameters that executed the plan of stage construction. This study analyzes consolidation behavior of overconsolidated clay with two-dimensional consolidation analysis program by use of the numerical method. By using this program, the time dependent consolidation behavior of soft deposits can be analyzed appropriately. Elastic and elastic-plastic finite element analysis are compared with Terzaghi's theory of one-dimensional consolidation.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

중증 재생불량성 빈혈 환자의 동종골수이식 후에 병발한 간정맥폐색질환(Hepatic Veno-Occlusive Disease)의 임상 경과 및 혈액응고 인자들의 변화

이제환,이규형,최성준,민영주,김신,이정신,김상희,박찬정,지현숙,김우건 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

연구배경: 재생불량성 빈혈 경우에는 조혈모세포 이식 후 VOD의 발생에 관한 보고가 거의 없는 실정이다. 저자 등은 cyclophosphamide와 antithymocyte globulin으로 전처치 후에 동종골수이식을 시행한 재생불량성 빈혈 환자들을 대상으로 하여 VOD의 발생과 임상 양상, 혈액응고 인자들의 변화에 관하여 분석하여 보았다. 방법: 재생불량성 빈혈로 동종골수이식을 시행 받은 13예 환자를 대상으로 하였다. 임상적 기준에 따라 VOD를 진단하였으며 임상 경과에 따라서 경중을 분류하였다. Protein C, protein S, antithrombin Ⅲ, tPA, PAI-1 등의 혈액응고 인자들을 전처치 투여전, 골수 주입당일, 이식 후 제7, 14, 21일에 측정하였다. 결과: 7예(53.8%)에서 VOD가 발생하였으며, 경증 5예, 중등도 2예였다. VOD군에서 혈소판치가 20.000/㎕ 이상을 유지하는 데까지 걸리는 시간이 더 길었다(p=0.026). 또한 VOD군에서 적혈구 및 혈소판의 수혈 요구량이 각각 유의하게 많았다(p=0.025, 0.015). 혈액응고 인자들의 변화를 보면, 제 0일에 VOD군에서 PAI-1의 혈중 농도가 유의하게 더 높았다.(p=0.034). 결론: 재생불량성 빈혈환자에서도 조혈모세포 이식 후에 VOD의 높은 발생률을 관찰하였으며, 대부분이 경증이었다. VOD 환자에서의 혈액응고 인자들의 변화는 PAL-1이 VOD의 감별 진단을 위하여 이용될 수 있음을 시사하고 있다. Background: There are few reports about the occurrence of hepatic veno-occlusive disease(VOD) after bone marrow transplantation (BMT) for severe aplastic anemia (SAA). We analyzed the incidence and clinical outcomes of VOD and changes of coagulation factors in patients with SAA who were conditioned with cyclophosphamide plus ATG and underwent allogeneic BMT. Methods: Thirteen patients with SAA who underwent allogeneic BMT were included. A diagnosis of VOD was made according to clinical criteria, and severity of VOD was classified according to clinical course. Plasma levels of protein C, protein S, antithrombin Ⅲ, t-PA, and PAI-1 were determined before preparative chemotherapy, in the day of marrow infusion, and days 7, 14, and 21. Results: VOD occurred in 7/13(53.8%). Five were mild VOD and two were moderate VOD. Time to platelet ≥20,000/L was slower (p=0.026) and red cell and platelet transfusion requirements were higher (p=0.025,0.015) in patients with VOD . The mean plasma level of PAI-1 at 0 were higher in patients with VOD (p=0.034). Conclusion: We observed the high incidence of VOD after allogeneic BMT in patients with SAA. A majority of cases of VOD were mild. The changes of PAI-1 after BMT may be useful marker of hepatic VOD

한국인 젊은 여성의 심미적인 안면 연조직 형태에 관한 두부 X-선 계측학적 연구

우제경,성재현,권오원 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.2

본 연구에서는 대구에서 활동하고 있는 모델과 심미적인 안모를 가졌다고 추천받은 68명의 젊은 여성을 대상으로 하여 두부X-선 사진, 정모 사진과 측모 실루엣 사진을 채득하였다. 두부X-선 사진의 연조직 측모선만을 사용한 3명씩의 측모 실루엣을 FH plane이 평행하게 한 장에 그려서 제작한 사진을 경북대학교 미술학과 재학생 7명과 교정 전공의 15명에게 평가하게 하였으며 정모 사진을 교정 전공의만 평가하게 하였다. 아주 좋은 경우를 9점, 좋은 경우를 7점 보통인 경우를 5점, 좋지 않은 경우를 3점으로 처리토록 하였다. 측모 실루엣에서 교정 전공의와 미대생의 심미안간의 상관관계, 교정 전공의가 평가한 정모와 측모의 심미성간의 상관관계, 교정 전공의가 평가한 측모 실루엣의 평균 점수가 5점 이상인 38명의 측모 계측항목과 측모 심미성간의 상관관계를 분석하여 다음과 같은 결론을 얻었다. 1. 측모 실루엣에서 교정 전공의와 미대생의 심미안간의 상관관계는 유의성있게 나타났다(r=0.67, P=0.0001). 2. 교정 전공의가 평가한 정모와 측모의 심미성간의 상관관계는 유의성있게 나타났다(r=0.26, P=0.0381). 3. 측모 실루엣과 상관관계가 유의성있게 나타난 항목은 Na-Pog to N′, BNV to Pog′, BNV/B′-Pog′, Ls-Li-Pog′, Li-B′-Pog′, Z angle이었다.(P<0.05). 4. 측모 계측항목의 평균치 및 표준편차를 구하였다. Cephalometric radiographs, frontal photographs and profile silhiuette phogographs of 68 young adult female who were model or were recommended to have esthetic face were used in this study. 7 Students in department of Art of Kyungpook national university and 15 orthodontists estimated profile slides which were made of 3 profile silhouettes in parallel with FH plane. Profile silhouettes were made of soft tissue profile line of cephalometric radiograph. Only orthodontists estimated frontal photographs. Students and Orthodontists score 9 in excellent case, score 7 in good case, score 5 in average case, score 3 in poor case. Correlation analysis between orthodontists` esthetic concept and Artists` esthetic concept, between frontal view esthetics and profile view esthetics which estimated by orthotontists. between profile view esthetics and profile measurements which consisted of measurements of 38 female who were scored above 5 mean score in profile silhouette by orthodontists were done. And the finding in this study indicated the following 1. Correlation between orthodontists` esthetic concept and Artists` esthetic concept in profile silhouette was significant(r=0.67, P=0.0001). 2. Correlation between frontal view esthetics and profile view esthetics which estimated by orthodontist was significant(r=0.26, P=0.0381). 3. Measurements which had significant correlation between profile measurements and profile view esthetics were Na-Pog to N′, BNV to Pog′, BNV/B′-Pog′, Ls-Li-Pog′, Li-B′-Pog′and Z angle(P<0.05). 4. Mean and standard deviation of profile measurements of 38 female were obtained.

조혈모세포이식 후 골성장인자의 변화 및 골대사에 미치는 영향 : Impact on Bone Mineral Metabolism

백기현,오은숙,오기원,이원영,김혜수,권순용,한제호,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: 각종 장기이식의 시행이 많아지고 이식 후 생존율이 증가함에 따라 이식 후 합병증에 대한 관심 또한 높아지고 있다. 조혈모세포이식 후에도 다양한 내분비적 합병증이 발생할 수 있으며 골격에 대한 합병증도 문제점으로 대두되고 있다. 조혈모세포 이식 후 발생하는 골소실에는 이식 후 초기의 골형성 저하와 골흡수 증가가 중요한 역할을 담당하리라고 추측되는데 이러한 골재형성불일치(biochemical uncoupling)에 골 성장인자들이 미치는 영향에 대해서는 알려진 바가 없다. 본 연구에서는 조혈모세포이식 전, 후로 말초 혈액에서 IGF-I, FGF-2, M-CSF같은 성장인자의 변화를 알아보고, 이들 성장인자의 변화가 조혈모세포이식 후의 골형과 골흡수에 미치는 영향 및 이식 후 발생되는 골량 소실과의 연관성을 확인해 보고자 하였다. 방법: 여러 가지 혈액질환으로 인해 동종 골수이식을 시행 받은 환자들을 전향적으로 관찰하였으며 이식 전 및 이식 후 1주, 2주, 3주, 4주 및 3개월, 6개월 1년에 말초 혈액에서 골교체표지자를 측정하였다. 이식 전 및 이식1년 후에 요추골 및 대퇴골 골밀도를 측정할 수 있었던 36명의 환자들을 대상으로 냉동 보관되어 있던 혈청을 이용하여 IGF-I, FGF-2 및 M-CSF를 시기별로 측정하였으며 이들 성장인자와 골교체표지자의 변화 및 골밀도 변화 사이의 상관관계를 확인하였다. 결과: 골흡수 표지자인 혈청 ICTP는 이식 전에 비해 이식 후 4주까지 점차 의의 있게 증가하다가 이후 6개월까지 더욱 증가한 후 감소하였다. 골형성 표지자인 osteocalcin은 이식 후 3주까지는 점차 감소하다가 이후 증가하여 이식 후 3개월 및 6개월에 기저치보다 통계적으로 유의하게 증가한 후 감소하였다. 혈청 IGF-I과 FGF-2는 각각 이식 후 3주 및 1주까지 의미있게 감소하다가 이후 증가하였으며 혈청 M-CSF는 이식 후 1주째에 기저치에 비해 의미 있게 증가하였다가 이후 기저치로 회복되었다. 이식 1년 후 평균 요추부 골밀도는 5.2% 감소하였고 평균 근위대퇴골 골밀도는 11.6% 감소하였다. 이식 전 및 이식 후 3주에 측정한 IGF-I과 같은 시기에 측정한 오스테오칼신 사이에 유의한 상관관계가 관찰되었으며 이식 후 3주째의 M-CSF와 골흡수표지자인 M-CSF 사이에서 의미 있는 양의 상관관계를 관찰할 수 있었다. 이식 후 3주 및 3개월에 IGF-I이 낮은 환자일수록 이식 1년 후 근위대퇴골에서의 골소실이 많은 것으로 분석되었다. 결론: 조혈모세포이식 후 발생하는 골소실에는 기존에 알려진 기저질환의 영향, 성호르몬의 감소, 면역억제의 투여, 골수기질세포와 조골세포의 손상 및 이식초기 사이토카인의 변화이외에도 골성장인자가 관련이 있음을 확인하였고, 이는 이식 후 발생되는 골량소실에 중요한 역할을 할 것이라고 사료된다. Background: A loss of bone mass is usually detected after a bone marrow transplantation (BMT), especially during the early post-transplant period. We recently reported that enhanced bone resorption following a BMT was related to both the steroid dose and the increase in IL-6. We also suggested damage to the marrow stromal microenvironment, by myoablation, partly explains the impaired bone formation following a BMT. It is well known that some growth factor play important role in bone growth and osteogenesis. However, the pathogenetic role of bone growth factors in post-BMT bone loss is unknown and data on the changes in the growth factors, in accordance with bone turnover markers and bone mineral density (BMD) changes are scarce. We investigated changes in bone growth factors such as IGF-I (Insulin-like growth factor-I), fibroblast growth factor-2 (FGF-2) and Macrophage colony stimulating factor (M-CSF), during the post-BMT period, and assessed whether the growth factor changes influenced the bone turnover and post-BMT bone loss. The present study is the first prospective study to describe the changes in bone growth factors following a BMT. Methods: We prospectively investigated 110 patients undergoing a BMT, and analyzed 36 patients (32.4±1.3 years, 17 men and 19 women) whose BMDs were measured before, and 1 year after, the BMT. The serum biochemical markers of bone turnover were measured before, 1, 2, 3 and 4 weeks, 3 and 6 months, and 1 year, after the BMT. The serum, FGF-2, IGF-I and M-CSF levels were measured before and 1 and 3 weeks, and 3 months after the BMT. The correlation between the changes of growth factors and various bone parameters was analyzed. Results: The mean bone losses in the lumbar spine and total proximal femur, calculated as the percentage change from the baseline to the level at 1 year, were 5.2(p<0.05) and 11.6%(p<0.01), respectively. the serum type I carboxyterminal telopeptide(ICTP), a bone resorption marker, increased progressively until 6 months after the BMT, but thereafter decreased, to the base value after 1 year. Serum osteocalcin, a bone formation marker, decreased progressively, until 3 weeks after the BMT but then increased transiently, and finally returned to the base level at 1 year. The serum IGF-I and FGF-2 also decreased progressively until 3 weeks 1 week after the BMT, respectively, then increased to the base values at 3 months. The serum M-CSF increased briskly at 1 week post-BMT, then decreased to the base level. There were positive correlations between the percentage changes from the baseline proximal femur BMD and the IGF-I levels 3 weeks and 3 months (r=0.52, p<0.01, r=0.41, p<0.05) post BMT. A significant correlation was found between the IGF-I and osteocalcin levels pre-BMT, and 3 weeks after the BMT. Another positive correlation was found between the M-CSF and the ICTP levels at 3 weeks post BMT (r=0.54, p<0.05). Conclusion: In conclusion, there were significant changes in the serum IGF-I, FGF-2 and M-CSF levels in the immediate post-BMT period, which were related to a decrease in bone formation and loss in the proximal femoral BMD during the year following the BMT (J Kor Soc Endocrinol 17:664∼674, 2002).

들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

비탈형 거푸집의 경제성 향상에 관한 연구

조규현,김제섭,전판근,김우재,정상진 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study is to improve the productivity and constructability of permanent forms. The permanent forms will cut down the cost of production and make easier to install in the field; hence increase the use of forms in the today's market. The permanent form is produced in the factory and built in field. So this study is in two parts. The one is the productivity in factory, the other is constructability in the field. This study is the designing a new mold system as a solution to existing mold system's problems.

윤곽선 추적을 이용한 도면 영상의 효율적인 압축

조형제,유성현 東國大學校 1996 東國論叢 Vol.35 No.-

Drawing images consist of many line segments and their line segments have width informations. The compression method of drawing images of previous works using skeletonizing has lots of problems; the loss of width information, unfair representation at part of branching point. et. al. In this paper, the compression of drawing images using contour tracing is proposed for the purpose of overcoming above defects. During tracking outline of line segments, outline is split into many straight lines and many curved parts. Then straight lines are represented by start and end points, and curved parts are approximated to Bezier curves and represented by Bezier control points. In the experiment, compression rate of 96% was obtained. And the image enlarged by scaling the representative parameters of this method looked more sleek than the image magnified simply by pixel-by-pixel.

급성골수성 백혈병에서 CD34와 P-당단백의 발현

이제환,김우건,김상위,이정신,이규형,장대영,최종수,김상희,김성배,서철원,지현숙 울산대학교 의과대학 1995 울산의대학술지 Vol.4 No.1

Backgrounds : The expression of the MDR-1(multidrug resistance)encoded P-170 glycoprotein(p-170)and CD34 have been well known to be associated with drug resistance in AML(acute myelogenous leukemia). P-170 and CD34 expression in AML have been reported as unfavorble prognostic parameters separately. Methods : P-170 glycoprotein expression was analyzed in correlation with CD34 expression and clinical response in 15 consecutive patients with de novo acute myelgenous leukemia (AML). They were measured with flow cytometry after direct and indirect immunofluorescence staining simultaneously. Results : 1) The positive rate of P-glycoprotein and CD34 were in two of 15 patients(13%), seven of 15 patients(46%), respectively. 2) One of two P-170 positive patients as compared with 7 of 13 P-170 negative patients achieved a complete remission(CR), which showed no clinical significant difference. 3) There was no significant correlation between P-glycoprotein and CD34 expression(r=0.29, p=0.28). 4) In de novo acute myelogenous leukemia, there was no case which expressed both P-170 and CD34 simultaneously. 5) P-glycoprotein and CD34 were not expressed in acute promyelocytic leukemia group. 6) Cytogenetic abnormalities did not show any significant difference in the rate of P-glycoprotein expression, CD34 expression and complete remission. Conclusion : CD34 and P-glycoprotein in acute myelogenous leukemia were independent parameter in this study. Further investigations are warranted for clinical implication.