김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

보문 : Polyethylene Film포장이 포도 Sheridan의 저장력에 미치는 영향

남상영(Sang Young Nam),김경미(Kyoung Mi Kim),박종천(Jong Chun Park),주선종(Seon Jong Joo),정재현(Jai Hyun Jung) 한국식품저장유통학회(구 한국농산물저장유통학회) 1997 한국식품저장유통학회지 Vol.4 No.1

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

고등학교 유도팀의 집단응집력이 성원만족에 미치는 영향에 관한 연구

정훈, 김종필, 최종균, 김병주 용인대학교 체육과학연구소 2000 體育科學硏究論叢 Vol.10 No.1

The purpose of this study is to verify hypothecial model through identifying the influence of high school judo athletics team's cohensiveness on member satisfaction. The subjects of this study were 157 members in 6 high school judo teams in Korea. The data collecting instruments which were used by the several precedent researchers were utilized to collect data which validity and reliability have been established via proper procedures. The data was analized through correlation analysis. Multiple regression analysis, and path analysis using multiple regression analysis to test statistical significance with the significance level of .05, .01, .001. The major findings based on the data analysis were as follows. The results obtained from this study are as follow; First, personal-social cohensiveness(β=.106) of judo team affecting on game satisfaction a significant differences statistically. Second, personal-social cohensiveness(β=.138), group-task cohensiveness(β=-.078), peisonal-task cohensiveness(β=.086), group-task cohensinveness(β=.096) of judo team affecting on member satisfaction a significant differences statistically. Third, personal-social cohensiveness(β=.286), group-task cohensiveness(β=.183) of judo team affecting on belonging satisfaction a significant differences statistically.

고속액체크로마토그래피에 의한 나프록센 광학이성질체의 분리 및 품질관리

정수진,진전성,이은주,이은실,강종성 忠南大學校 生命科學硏究院 醫藥品開發硏究所 2006 藥學論文集 Vol.21 No.-

Enantiomeric separation of naproxen is necessary in drug evaluation processes as the pharmacological activity of naproxen resides mainly in the (S)-enantiomer, The enantioseparation of naproxen was carried out with three different stationary phases. The methods were validated with the terms of linearity, intraday and interday precision, accuracy, selectivity and recovery. Among the selected stationary phases, ChiroSil DMB column with hexane : t-butylmethylether : acetic acid (60:40:0.1, v/v/v) as mobile phase showed the best enantioseparation of the naproxen in the commercial preparations. The wavelength for detection was 270 nm and flow rate was 2.0 mL/min. The contents of (S)-naproxen in the commercial preparations were 92.7 - 98.3%, indicating that all tested preparations were satisfactory to current regulation. However, the contents of (R)-naproxen as impurity in the commercial preparations were varied from 0.08% to 1.08%.

부산시 공동주택 폐기물의 발생특성 및 비용분석

정대순,이종현,박태주 부산대학교 환경문제연구소 1999 環境硏究報 Vol.17 No.1

This study was conducted to evaluate the solid waste characteristics and collection and transportation cost in apartment buildings in Pusan city. Eight apartments at six districts were selected as survey area for this study and sample waste generated from apartment buildings were collected three times. Solid waste generation rate of apartment building was 0.68 ~0.81 kg/capita · day which is lower than average generation rate in Pusan city and the whole country. Composition of apartment buildings wastes were papers 20.9%, foods; 48.8%, plastics; 5.0%, miscellaneous organic solids; 9.9%, respectively. Incombustible materials such as, glass and ceramics, metals and ashs were found in the range of 0.7% to 20.7% of the solids. Collection and transportation cost per kg of refuge were varied in the range of 60.3~76.9 won. Crew size, container location. type of truck, collection route and travel time were significant factors in determining the cost of waste collection.

도덕 교육의 방법론 : 가치명료화 도덕교육의 이론과 수업적용 The Theory of Value Clarifying Moral Education & Its Classroom Application

정보주,권종규 晋州敎育大學校 初等敎育硏究所 1993 初等敎育硏究 Vol.3 No.-

백서 해마에서 카이닌산에 의한 조기유전자의 발현과 p42, p44 MAPK 및 EIK-1 인산화의 발달 단계에 따른 변화

정희연,김수진,김종흔,정선주,박주배,김용식,조수철 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

연구목적 : 어린 백서에게 카이닌산(kainic acid, KA)을 주사하여 발작을 일으킨 후, 해마에서 조기유전자 -c-fos, junB, 및 TIS1의 발현 유도 양상을 발달 단계별로 조사하여 전기경련충격(electroconvulsive shock, ECS)에서의 결과와 비교함으로써 백서 뇌 신호전달계의 성숙과정을 파악하고자 하였다. 그리고 KA 주사후 p42, p44 mitogen activated protein kinase(MAPK)의 인산화 및 그에 의해 활성화되는 것으로 알려진 전사인자 Elk-1의 인산화를 관찰하여, KA 주사후 MAPK 신호절달계를 통한 c-fos의 발현로 알려진 전사인자 Elk-1의 인산화를 관찰하여, KA 주사후 MAPK 신호전달계를 통한 c-fos의 발현 경로를 발달 단계별로 밝히고자 하였다. 방 법 : 생후 7, 14 21일 된 수컷 백서에서 KA를 복강내 주사한 후, 백서 해마에서 조기유전자의 발현 양상은 northern blot analysis로, p42, p44 MAPK와 Elk-1의 인산화는 immunoblotting으로 관찰하였다. 결 론 : 생후 7일된 백서의 해마에서는 ECS와는 달리, KA에 의한 세 가지 조기유전자의 발현이 관찰되지 않았다. 그러나 생후 14일부터 이들 조기유전자의 뚜렷한 발현을 관찰할 수 있었고 생후 21일에는 성숙한 백서와 같은 수준의 발현 양상을 볼 수 있었다. 세 가지 유전자 모두 백서의 연령과 KA 주사 후 시간에 따른 발현 유도 양상은 비슷하였다. p42, p44 MAPK는 생후 7일부터 상당한 수준의 기저치 인산화가 관찰되었으나, KA에 의한 인산화 증가는 생후 14일부터 관찰되었다. Elk-1의 인상화 역시 생후 7일부터 높은 수준으로 관찰되었으나 KA 주사 후 시간에 따른 Elk-1 인산화의 변화는 관찰할 수 없었다. 결 론 : ECS와 KA가 조기유전자 발현 양상에 차이를 보이는 것은 이들 유전자의 발현과 관련되어 활성화되는 신호전달경로의 차이 때문으로 생각된다. 백서 해마에서 KA에 의한 MAPK 활성화에 관여하는 신호 전달 기구는 연령이 높아짐에 따라 점차 성숙하고, MAPK의 활성화로 전달된 신호는 Elk-1 이외의 다른 경로를 통해 c-fos 발현을 조절하는 것으로 추측된다. Objectives : In order to investigate the maturational process of intracellular signal transduction system in rat brain, we studied the induction of the immediate early genes(IEGs)-c-fos, iunB, and TIS1 in each developmental stage after kainic acid(KA)-induced seizure in young rat hippocampus and then compared these with the results after electroconvulsive shock(ECS) And to elucidate the induction mechanism of c-fos via mitogen-activated protein kinase(MAPK) by KA in each developmental stage, we investigated the phosphorylation of p42, p44 MAPK and Elk-1 after KA treatment in young rat hippocampus. Methods : We examined the induction patterns of IEGs by northern blot analysis, and the phosphorylation of p42, p44 MAPK and Elk-1 by immunoblotting in rat hippocampus at post-natal day 7, 14, and 21(P7, P14 & P21), respectively after intraperitoneal injection of KA. Results : Unlike ECS, KA did not induce c-fos, junB, and TIS1 in P7 hippocampus. But these genes were apparently induced at P14 and to an adult level at P21. These three IEGs showed similar temporal patterns of induction in each developmental stage. Although the basal level of phosphorylated 42p, 44p MAPK was considerable in P7 rat hippocampus, the increase or phosphorylation after KA treatment was observed at P14. While the phosphorylation of Elk-1 was detected with high basal level in P7 rat, the amount of phosphorylated Elk-1 was not changed after KA treatment. Conclusion : Our results suggest that the differences in IEGs induction patterns between KA and ECS may be due to the differences in the activated signal transduction pathways. And our results also implicate that the signal transduction system involved in MAPK phosphorylation after KA treatment mature with aging and c-fos induction via MAPK activation may be regulated through some pathways other than Elk-1 in rat hippocampus.

?茶의 吸濕特性

李周伯,鄭信敎,孫泰華,崔鍾旭 慶北大農學誌編輯委員 1986 慶北大農學誌 Vol.4 No.-

市販 ?茶를 20℃에서 相對濕度를 달리하여 저장하면서 吸濕特性을 조사하였다. ?茶의 吸濕速度는 貯藏相對濕度가 높을수록 컸으며 貯藏時間이 경과함에 따라 점차 감소하였다. ?茶의 等溫吸濕曲?은 전형적인 Sigmoid 형태를 나타내었으며 相對濕度가 일정할 때 저장시간과 흡습속도와의 관계에서 다음과 같은 式을 구할 수 있었다. ln(dw/dt)=nln(t) + ln c B.E.T. 式에 의해 구한 ?茶의 單分子層水分含量은 7.01~7.87%로 나타났다. 貯藏時間과 吸濕速度關係式에서 구한 계산치 수분함량과 실측치수분함량을 비교한 결과 거의 유사한 경향이었다. The sorption characteristics of green tea were investigated at 20℃ with various relative humidities. Particle size showed little effect on the sorption behavior of green tea. At low relative humidities below 57%, the sorption equilibrium were easily attained, but a high relative humidities above 75%, the sorption equilibrium were not reached after 10 days. From the estimation of sorption rate at arbitrary humidities an empirical equation was obtained; Ln(dw/dt)=nLn(t) + Ln c The monolayer moisture contents of green tea obtained by B.E.T. equation were found to be 7.87%(powder) and 7.01%(whole), respectively.

1998년과 2007년 사이 12∼13세 남녀 청소년의 체격, 골격성숙도, 체력 비교

송종국(Jong Kook Song),채서연(Seo Yeon Chae),채주희(Joo Hee Chae),김현배(Hyun Bae Kim),강효정(Hyo Jung Kang) 한국사회체육학회 2008 한국사회체육학회지 Vol.0 No.34

The purpose of the present investigation was to compare physique, skeletal maturation and physical fitness in Korean adolescents measured between 1998 and 2007. Subjects participated in the study were a total of 473 Korean adolescents aged 12 to 13 years. In 1998, 349 adolescents were selected from a middle school, located in Sungnam city and 124 middle school students aged 12-13, located in Yongin city were participated in 2007. All measurements were conducted by one of the reseachers (Jong-Kook Song) with two different group of investigators between 1998 and 2007. Anthropometric characteristics were body mass, body height, sitting height, circumferences taken at sites of upper arm, flexed upper arm, chest, waist, hip, thigh, and calf, bone breadth taken at the sites of humerus, and femur, skinfold thicknesses taken at the sites of triceps, subscapular, suprailiac, thigh and medial calf. Skeletal maturity was assessed by TW3 (Tanner et al., 2001). Maturity scores for Radius-Ulna-Short bones (RUS) and bone age were calculated. Physical fitness parameters included flamingo balance, sit and reach, grip strength, sit-ups, standing long jump, bent arm hang, 50m shuttle run and multi-stage shuttle run. All data were analyzed by SAS Enterprise 3.0. Means and standard deviations were calculated by descriptive statistics. Independent t-test was applied to compare dependent variables between measurements. The level of significance was set at p<0.05. Results of the study showed that male adolescents aged 12-13years in 2007 had significantly higher (p<0.0001) femur diameter than adolescents in 1998. Male adolescents in 2007 had higher (p<0.05) chest, waist and calf circumferences than those of adolescents in 1998. Although no difference were found for RUS and bone age in males adolescents between 1998 and 2007 female adolescents in 2007 were significantly delayed for their skeletal maturity level compared to female adolescents in 1998. For physical fitness parameters male adolescents aged 12 years in 2007 performed better for flamingo balance (p<0.01), and 50m shuttle run (p<0.05) than those of adolescents in 1998. However, stand long jump and bent arm hang were significantly decreased in male adolescents in 2007. Female adolescents aged 12-13 years in 2007 had significantly higher flamingo balance, but female adolescents aged 12 years in 2007 performed significantly less for standing long jump (p<0.0001), bent arm hang (p<0.001) and speed (p<0.05) than those of female adolescents in 1998. In conclusions this study suggest that chest circumference for both male and female adolescents in 2007 was significantly higher than those in 1998. Skeletal maturity for female adolescents in 2007 were significantly delayed compared to female adolescents in 1998. Further study is needed to evaluate consistent various.