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서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)
김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1
In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.
Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands
정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3
침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.
지하수관리시스템 구축을 위한 전산모형과 지리정보시스템의 통합
김준현,김정욱,한영한 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A
GIS is linked with Numerical Model(PM5) for the effective conservation and management of groundwater resources. All the relevant system was systematically constructed to analyze the field status by means of precise understanding of the surface and subsurface environment and by accumulating large amounts data and attribute data into developed system. The necessity of integrated management of the groundwater information was demonstrated and the general direction of the construction of groundwater management system was suggested. The proposed system will guide a method for the rational decisions of groundwater development via the establishment of the appropriate database management and accurate prediction of future change.
HPLC를 이용한 농약 Carbendazim의 측정법에 관한 연구
김준서,조만희,이상한,김창세 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1
The measurements of pesticide Carbendazim were studied and following conditions were proposed as a most good methods; 1. There were detected at 204 nm by means of Hitachi 200-20 spectrophotometer. 2. Pesticide Carbendazim mesurement was performed by menas of ISCO model 2350, spherisorb ODS column, 70% acetonitrile as a solvent phase, flow rate 0.5 ml/min, chart speed 0.5 cm/min, and 25℃ oven temperature. 3. The relationships between the concentration and absorbance of pesticide Carbendazim were linear from 2.5 to 15 ppm. 4. It showed stable result in Folin-Wu, and the recovery rate was also good.
김창윤,이철,안준호,박인호,한오수 울산대학교 의과대학 1995 울산의대학술지 Vol.4 No.2
The diagnostic tools and treatment modalities for epilepsies are growing in recent years. Therefore many investigators are studying the relationship of ipilepsy and psychopathology. These efforts are not only for the understanding of and therapeutic approach to psychiatric disorder in epileptic patients but also seeking the etiological basis of psychopathologies. Method 45 patients who were diagnosed as temporal lobe epilepsy by brain MRI and EEG etc were investigated, and offered psychiatric diagnosis. Also we took detailed psychiatric histories and investigated the characteristic features of psychiatric disorders in temporal lobe epilepsy. We evaluated several variables, i.e., age, sex, duration of epilepsy, age at first seizure, age at onset of epilepsy, time between first seizure and onset of epilepsy, seizure frequency, history of febrile convulsions, history of clustering seizures, and laterality of focus of these 45 subjects. Futher investigations divided 45 subjects into a group without psychiatric disorder, a group with mood disorder and a group with psychotic disorder. And we compared those variables among these 3 groups. Among 45 subjects, nine were diagnosed as psychotic disorders, five were mood disorders, and two were anxiety disorders. Among 9 subjects with psychotic disorders, six were diagnosed as psychotic disorder NOS, and the other three were schizophrenia. And they manifested unique clinical features in relations to epileptic seizures. Among 5 subjects with mood disorders, 4 subjects were diagnosed as dysthymia with chronic depression and 1 subject were major depression. We compared all variables between the group without psychiatric disorder and the group with psychotic disorder however we can't find any statistically significant differences except one variable. In the group with psychotic disorder, all subjects had the history of clustering of seizures in the rate of 100% of cases, but 54.6% of cases of the group without psychiatric disorder. The statistical difference was significant(p=0.016). It is a preliminary study in this field in Korea. However this study will help to understand the various psychiatric symptoms of the patients with temporal lobe epilepsy and will help to promote further investigations in this field.
춘천시의 지하 저장 탱크의 예비적 위해성 평가를 위한 설치 현황 분석 및 지리정보시스템의 적용
김준현,한영한,이종춘,권영성,이광연 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.A
In this study, the preliminary risk assessment for the underground storage tanks(UST) in Chunchon city was implemented using the geographical information system(GIS). The estimation variables, such as the installation year, storage capacity, the distances from streams, and from groundwater pumping wells, were selected to estimate the relative risk levels. The weighting factors were given to all the estimation variables. Cumulative scores were induced by the combination of al1 the scores of the corresponding variables using the buffering technique and the overlay analysis in ArcView. Using the these process, the relative risk level of each UST was estimated. Some sites in this study are simplified and reduced because the number of useable data are limited or too enormous. Thus the selection of the comprehensive estimation variables and the proper weighting values are required for the future study. The methodology in this study could be served not only for the preliminary risk assessment of UST but also for the selection of the proper location of new and old UST. And, it can be used for the effective management system of UST.
김한성,최현준,주미,장선희,곽지은,김철남 인제대학교 2006 仁濟醫學 Vol.27 No.-
Typical serous cystadenoma of pancreas is formed by many microcysts, of which diameter are less than 2cm. Recently, rare variants are also reported as macrocystic, unicystic, or multicentric. Macrocystic serous cystadenoma is diffcult to differentiate from pseudocyst, mucinous cystadenoma and other cystic mass having macrocysts. As these lesions have different symptoms, methods of treatment, and prognoses, it is important to diagnose and treat correctly. We report 2 cases which diagnosed as macrocystic serous cystadenoma with stressing scarcity value and clinical importance.