Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

일부 성인의 치과 공포감 관련 요인

김수경 ( Soo Kyung Kim ),김미희 ( Mi Hee Kim ),최현지 ( Hyun Ji Choi ),황정근 ( Jung Geun Hwang ) 한국치위생학회(구 한국치위생교육학회) 2014 한국치위생학회지 Vol.14 No.6

Objectives: The purpose of the study was to investigate the related factors to dental fear in some adults. Methods: A self-reported questionnaire was filled out by 320 adults in Seoul and Gyeonggi-do from April to June, 2013. Except 14 incomplete answers, data were analyzed by SPSS 18.0 program. The questionnaire consisted of general characteristics of the subjects(7 questions), dental fear related factor(18 questions), distrust for dentist(15 questions), and distrust for dental hygienist(11 questions). The instrument for dental treatment fear was Dental fear Survey(DFS) adapted from Kleinknecht and partly modified by Choi. Out of 20 questions, 18 questions were reconstructed and score by Likert 5 scale. Higher score means higher dental fear. Cronbach alpha was 0.959 in the study. The distrust for dentist and dental hygienist was adapted from Choi and reconstructed and score by Likert 5 scale. Higher score means higher distrust for dentist and dental hygienist. Cronbach alpha was 0.937 in distrust for dentist and 0.874 in distrust for dental hygienist in the study. Results: Those who experienced dental pain tended to have dental fear. The reasons for dental fear were as follows; bad breath(2.96 out of 5 points, hereafter represented as of 2.96/5), dental caries(2.88/5), missing teeth(2.87/5) and tooth pain(2.77/5). The distrust for the dentist was the main dental fear in the adults. Conclusions: The dental fear was closely related to gender, experience of dental pain, oral symptoms and distrust for the dentist. As the psychological pain in the patients was mainly influenced by the trustful atmosphere, it is necessary to make the patient easy before treatment.

중부지역 대학생의 남녀평등의식에 관한 실태조사

최덕경,최동숙,강기정,유을용,변미희 한국청소년복지학회 2004 청소년복지연구 Vol.6 No.1

This study investigated gender eqalitarianism of university students. The purpose of this study was to offer basic information and desirable direction for the education program of student's sex-role indentity. The data collection was based on Korean Questionnaire of Gender Equality Perception developed by Korean Women Development Center. Its reliabilitv(Cronbach α) was .84. In this study.982 university students were sampled in Kyunggi, Gangwon and Chungchong provinces. T-test and One-way ANOVA was conducted with the SPSS 10.0 version program. The major findings of this study were as follow: First, university student showed the highest gender eqalitarianism in educational life field. But they have the lowest in social and cultural life field. Second, male university students showed lower gender eqalitarianism than female university students. Third. student who is middle or last in brother order showed higher than one who is first or only son. Forth, student who is grown up in urban showed higher gender eqalitarianism than one in country.

B언어통역에서 인지적 요소의 역할과 습득

최미경 한국외국어대학교 통역번역연구소 2003 논문집 Vol.7 No.-

This paper describes the basic role of cognitive elements, which correspond to extra-linguistic elements, broadly speaking, in the process of interpreting into the B language (LB) (LB interpretation). Interpretation into one's B-language may sometimes seem something where perfection is unattainable, as it involves limits as a foreign language. Nevertheless, one should endeavor to make possible that which seems impossible. When students are faced with the difficulties of having to come up with the appropriate expression in B-language quickly, they tend to look for existing fixed expressions which come readily to mind. However, students should be taught to focus more on finding equivalence in sense which do not distort the message, rather than in sophisticated expressions. In particular, they should focus on thoroughly understanding the speech so that comprehension in their mother tongue and cognitive elements can compensate for the limitations in finding the appropriate wording in B-language. Students need to develop a habit of always analyzing and criticizing what has been learned or acquired in order to obtain a better understanding of cognitive complements.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

암모니아 산화 세균 Xanthomonas maltophilia SU6 와 Achromobacter sp. 12A에 의한 nitrite 생성의 비교

최미경,한효영,정영희,민경희 숙명여자대학교 환경과학연구소 1995 환경과학 Vol.2 No.-

암모니아를 NO_2^-로 산화, 이용하는 미생물 균주들을 한강 주변의 토양에서 분리하였으며, 이들은 Xanthomonas maltophilia SU6와 Achromobacter sp. 12A로 동정하였다. 이 균주들을 암모니아가 첨가된 액체 최소 배지에서 2주 동안 배양한 후, 생성되는 NO_2^-의 양을 측정하여 암모니아 산화의 활성을 조사하였다. 생장 최적 pH는 Xanthomonas maltophilia SU6 와 Achromobacter sp. 12A 균주가 7.0-8.0 이었으며, 생장에 필요한 무기질소원으로서 NH_4Cl 의 최적 기질 농도는 두 균주가 모두 10mM 이었다. 암모니아 산화 균주의 활성을 높이기 위하여 Xanthomonas maltophilia SU6와 Achromobacter sp. 12A를 12시간 배양한 후에 50mM의 hydroxylamine을 배지에 첨가하였을 때 각각 467㎍/1, 641㎍/1 의 NO_2^-를 유도 생성하였다. Hydroxylamine oxidase 의 활성에 미치는 기질의 영향을 규명하기 위하여 Xanthomonas maltophilia SU6 와 Achromobacter sp. 12A를 8시간 동안 암모니아가 포함된 최소배지에서 배양한 후에, hydroxylamine 을 첨가한 결과 hydroxylamine oxidase 의 비활성은 109units/㎎ protein 와 132 units/㎎ protein 으로 증가하였다. Hydroxylamine oxidase 의 최적 pH는 7.5, 최적 온도는 30℃ 였으며, 열에 대한 안정성에서는 Xanthomonas maltophilia SU6 와 Achromobacter sp. 12A 는 50℃, 60분에서 각각 30%와 25%의 안정성을 보여주었다.

51Cr방출과 MTT 및 LD활성도를 이용한 역충전재의 세포독성에 관한 연구

최라영,임미경 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.2

아동이 지각한 아버지의 양육행동 및 참여척도개발을 위한 기초조사 연구

최경순,오미경 新羅大學校 1991 論文集 Vol.32 No.1

The purpose of this study is to develop and validate the validity of the scale which measure father's child rearing behaviors and father's involvemint in child rearing perceived by their children. The subjects of this study are 1280 primary school children in Pusan. Questionnaires are used in order to collect data. The data were analyzed by t-test, F-test, Pearson's r, Duncan's Multiple Range test, factor analysis, Cronbach's α using SPSS computer program. The main results are as follows: 1. To validate the validity of the scale, primarily the mean, standard deviation, relative frequency distribution of the respective items, and Cronbach's α were computed. As the result, 32 items were selected in father's child rearing behaviors and 30 items were selected in the degree of father's involvement. Secondarily, the procedure of factor analysis was applied. consequently, 6 subscales 30 items, 4 subscales 27items were selected respectively. Total variance of 6 subscales and 4 subscale was 48.5% and 50.7% respectively. Reliability of scales was computed by Cronbach's α and the level was relatively considered satisfactory. Hence, subscales named runs as follow ; Father's child-rearing Behaviors Inventory consists of factorⅠ-'affection', factorⅡ-'interest', factorⅢ-'autonomy', factorⅣ-'achievement', factorⅤ-'academic guidance', factorⅥ-'expectation'. The Degree of the Father's Involvement Inventory consists of factorⅠ-'activity of family', factorⅡ-'paly partner', factorⅢ-'sociality guidance', factorⅣ-'household affairs'. 2. Practically, to applicate scale, difference test were conducted according to sex of the children, jobs of the parents, ages of parents, degree of the parent education, SES in father's child rearing behavior and father's involvement separately. As the result, there were variously differences according to demographic variables.