Aggravation of post-ischemic liver injury by overexpression of A20, an NF-κB suppressor

Yu, J.,Lee, H.S.,Lee, S.M.,Yu, H.C.,Moon, W.S.,Chung, M.J.,Park, J.W.,Park, B.H. Elsevier Science Publishers 2011 Journal of hepatology Vol.55 No.2

Backgroud & Aims: A20 is an intracellular ubiquitin-editing enzyme that plays an important role in the negative feedback regulation of NF-κB activation in response to a diverse range of stimuli. Liver ischemia/reperfusion injury is associated with rapid activation of NF-κB signaling, but the role of NF-κB in hepatic ischemia/reperfusion injury remains controversial. The NF-κB signaling pathway mediates both protective and deleterious effects in the liver. Here, we examined whether A20 inhibited or aggravated hepatic ischemia/reperfusion injury. Methods: We used IκBα super-repressor as a positive control and overexpressed A20 and IκBα super-repressor in the liver of C57BL/6 mice. Mice underwent 45min of partial hepatic ischemia and were then reperfused. Results: Protein level of A20 was increased after reperfusion. Mice subjected to ischemia/reperfusion injury showed increased NF-κB activation, as evidenced by phosphorylation of IκBα and nuclear translocation of NF-κB. Prior transfection with Ad-A20 or Ad-IκBα super-repressor attenuated NF-κB activation and aggravated liver injury. Serum aminotransferases and proinflammatory cytokines, hepatocellular necrosis, and hepatic neutrophil infiltration were markedly increased compared to those of uninfected or control virus infected mice. In addition, A20 abolished the beneficial effect of ischemic preconditioning. Conclusions: Our results suggest that inhibition of NF-κB activation by A20 aggravated partial hepatic ischemia/reperfusion injury. Understanding how the NF-κB pathway plays a role in directing a clinical outcome may lead to better prospects of more rational approaches to reduce post-ischemic liver injury.

1998년 분리된 이질균의 역학적 특성

이복권,강연호,김성한,유재연,전정훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

Inhibition of NF-κB-induced inflammatory responses by angiotensin II antagonists in aged rat kidney

Kim, J.M.,Heo, H.S.,Choi, Y.J.,Ye, B.H.,Mi Ha, Y.,Seo, A.Y.,Yu, B.P.,Leeuwenburgh, C.,Chung, H.Y.,Carter, C.S. Pergamon Press ; Elsevier Science Ltd 2011 Experimental Gerontology Vol.46 No.7

In this study, we explored the mechanisms by which the angiotensin converting enzyme inhibitor (ACEI), enalapril, and the Ang II receptor blocker (ARB), losartan suppress oxidative stress and NF-κB activation-induced inflammatory responses in aged rat kidney. The experimentations were carried out utilizing aged (24-month-old) Brown NorwayxFischer 344 (F1) male rats which were randomized into 3 groups and administered enalapril (40mg/kg), losartan (30mg/kg) or placebo for 6months (daily p.o.). The level of reactive species (RS), peroxynitrite (ONOO<SUP>-</SUP>), GSH/GSSG and lipid peroxidation were measured. The activity of the pro-inflammatory transcription factor NF-κB, and gene expression of proteins in upstream signaling cascades were measured by electro-mobility shift assay (EMSA) and Western blotting. Enalapril and losartan differentially attenuated redox imbalance and the redox-sensitive transcription factor, the NF-κB pathway. Furthermore, stimulation of the NF-κB activation pathway by phosphorylation of p65 was attenuated by both compounds. Moreover, mediation of phosphorylation of p65 by phosphorylation of IκB kinase αβ (IKKαβ) and mitogen- and stress-activated protein kinase-1 (MSK-1), were also inhibited by enalapril and losartan. Finally, both compounds also lowered expression of NF-κB-dependent inflammatory genes, such as cyclooxygenase-2 (COX-2), and inducible NO synthase (iNOS). Only losartan lowered levels of 5-lipoxygenase (5-LOX). These findings indicate that enalapril and losartan differentially suppress inflammatory responses via inhibition of oxidative stress-induced NF-κB activation in aged rat kidney.

돈골격근의 생화학적 특성에 관한 연구 1 . 환경온도가 돈골격근의 근원섬유 수축성과 Myosin B 추출성에 미치는 영향

유용걸(Y . G . Yu),김병태(B . T . Kim),이문준(M . J . Lee),박형기(H . K . Park) 한국축산학회 1990 한국축산학회지 Vol.32 No.2

The purpose of this study was to investigate the properties of myofibril and myosin B. Samples were extracted from the longissimus dorsi of lumber vertebra from the porcine weighing over 110kg in live weight. The porcine muscle was held either at 0℃ or at 40 ℃ for 30, 60, and 90 min. The carcass temperature immediatly after slaughter ranged from 42.0℃ to 42.6℃ with the line weight. The samples held at 40℃ showed much less contractility than those treated at 0℃. No contractility reaction was found on those treated for 90 min at 40℃. Extractibility of myosin B from the sample treated at 40℃ was lower than those treated at 0℃ The myofibril was not found in the residue of the sample treated at 0℃ which myosin B was extracted from treated at O℃ But there was much more myofibril in the residue from those treated for 60 min and 90 min at 40℃. Thus, the difference found in this experiment seemed at to reflect the differences in biological activities of the myofibrillar protein. As they corresponded to PSE porcine muscle symptoms, it can be concluded that the porcine carcass has to be held at 0℃immediately after slaughter in order to produce good quality pork especially from the animals exceeding 110kg in live weight.

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance

Jung, K. J.,Lee, E. K.,Kim, S. J.,Song, C. W.,Maruyama, N.,Ishigami, A.,Kim, N. D.,Im, D. S.,Yu, B. P.,Chung, H. Y. Springer Science + Business Media 2015 Journal of molecular medicine Vol.93 No.3

<P>Recent studies on senescence marker protein-30 (SMP30) have shown that it has an important functional role in the aging process, but its precise participation in cellular works has not been fully determined. We hypothesize that SMP30 plays crucial roles in signaling processes by modulating the balance of protein tyrosine kinase (PTK)/protein tyrosine phosphatase (PTP) and in activating proinflammatory NF-kappa B. An experimental paradigm of gain and loss of SMP30 function was established using SMP30-overexpressed YPEN-1 cells (herein referred to as 'SMP30(+) cells') and SMP30 (Y/-) knockout mouse kidneys. The resulting data show that SMP30 expression suppressed oxidative stress-induced PTK/PTP dysregulation and PP1/2A inactivation in SMP30(+) cells, leading to the suppression of NF-kappa B activation. In the kidneys of SMP30 (Y/-) mice, SMP30 deficiency was found to induce NF-kappa B activation via the upstream signaling of NIK/IKK and MAPKs and to upregulate downstream NF-kappa B-responsive gene expression. In this study, we also demonstrate for the first time that SMP30 deficiency induced PTK activity in SMP30 (Y/-) kidneys, thereby significantly increasing the tyrosine phosphorylation of a catalytic subunit of PP2A (PP2Ac-Tyr307). Based on these findings, we propose that SMP30 involves NF-kappa B regulation through the PTK/PTP balance and that the age-related decrease of SMP30 causes NF-kappa B activation, which contributes to an exacerbation of the inflammatory process during aging. Key message SMP30-deficient mice induced a shorter lifespan and redox changes. Overexpression of SMP30 prevented oxidative stress insults. The depletion of SMP30 increased redox-related PTK/PTP imbalance and PP1/PP2A inactivation. The depletion of SMP30 caused an elevation of NF-kappa B-responsive inflammatory markers. SMP30 may be a potent inhibitory protein against oxidative stress and chronic inflammation.</P>

An accurate simulation study on capacitance-voltage characteristics of metal-oxide-semiconductor field-effect transistors in novel structures

Yu, E.,Cho, S.,Park, B.G. North-Holland 2017 PHYSICA B-CONDENSED MATTER - Vol.521 No.-

<P>An essential and important method for physical and electrical characterization of a metal-oxide-semiconductor (MOS) structure is the capacitance-voltage (C-V) measurement. Judging from the C-V characteristics of a MOS structure, we are allowed to predict the DC and AC behaviors of the field-effect transistor and extract a set of primary parameters. The MOS field-effect transistor (MOSFET) technology has evolved to enhance the gate controllability over the channel in order for effectively suppressing the short-channel effects (SCEs) unwantedly taking place as device scaling progresses. For the goal, numerous novel structures have been suggested for the advanced MOSFET devices. However, the C-V characteristics of such novel MOS structures have not been seldom studied in depth. In this work, we report the C-V characteristics of ultra-thin-body (UTB) MOSFETs on the bulk Si and silicon-on-insulator (SOI) substrates by rigorous technology computer-aided design (TCAD) simulation. For higher credibility and accuracy, quantum-mechanical models are activated and empirical material parameters are employed from the existing literature. The MOSFET structure and the material configurations are schemed referring advanced logic technology suggested by the most recent technology roadmap. The C-V characteristics of UTB MOSFETs having a floating body with extremely small volume are closely investigated.</P>

Strain path effects on the microstructure evolution and mechanical properties of Zr702

Cao, W.Q.,Yu, S.H.,Chun, Y.B.,Yoo, Y.C.,Lee, C.M.,Shin, D.H.,Hwang, S.K. Elsevier 2005 Materials science & engineering. properties, micro Vol.395 No.1

<P><B>Abstract</B></P><P>A commercial-purity Zr702 was grain-refined from 20μm to 0.2–0.5μm by equal channel angular pressing. Grain refinement was most evident in the first pass but was insignificant during the subsequent passes. Two microstructural characteristics evolved: a lamellar structure and an equiaxed subgrain structure during the route A pressing and the route B<SUB>C</SUB> pressing, respectively. Due to the rotation of specimen in between passes, two sets of geometrically necessary boundaries were formed during the route B<SUB>C</SUB> pressing. The mechanism of grain refinement during the route A pressing was evolution of high-angle geometrically necessary boundaries from the low-angled ones, while that during the route B<SUB>C</SUB> pressing was decomposition and rearrangement of pre-existing boundaries. The yield stress of severely deformed specimens increased with the reducing grain size according to a Hall–Petch relationship.</P>

치즈 제조 부산물을 이용한 ELISA Blocking Agent의 개발에 관한 연구

유제현,송진욱,조흥찬,차광종,박범석,김응률,이종익,이중복,谷口孝喜 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

본 연구는 유청과 탈지분유를 blotto로 이용하여 ELISA 수행시 항원·항체반응에 비특이반응을 막는 Blocking agent를 탐색하기 위해 실험을 수행했다. MAb의 역가측정 결과 유의차가 인정되는 OD값이 0.3이상 검색된 희석배율은 1B2의 경우 819,200배, 6D4의 경우 1,638,400배, 15B10의 경우 204,800배이었다. 서로 다른 Blotto가 항원·항체반응에 미치는 영향을 각기다른 MAb를 이용하여 조사해 본 결과 MAb Yo-156, Yo-5는 Blocking agent로 표준적으로 사용하고 있는 BAS가 비교하여 group A 바이러스 7종 모두에 비슷한 정도의 발색을 일으켰으며, MAb G6는 G6 type바이러스 4종 모두에서 비슷한 정도의 발색을 일으켰고, MAb G10은 G10 type 바이러스 3종 모두에서 비슷한 정도의 발색을 일으켰다. 따라서 본 실험에 사용한 모든 종류의 blotto소재인, 탈지분유1(서울우유(협)), 탈지분유2((주)매일유업), 혼합탈지분유((주)매일유업), whey powder1(Sigma), whey powder2 ((주)삼익유가공), WPC34((주)환이상사), WPC800((주)후드피아), WPC8000 ((주)후드피아), WPI((주)환이상사)는 현재 Blocking agent로 사용하고 있는 BSA와 비교하여 유사하게 비특이 반응을 막아주는 것으로 밝혀져 경제적으로 보다 저렴한 blotto 소재로 이용이 가능하다는 사실을 확인하였다. Enzyme-Linked-Immuno-Sorbent-Assay (ELISA) has been frequently used for detection of interaction of antigen and antibody. Many efforts have been made to reduce nonspecific reaction of ELISA. Bovine serum albumin (BSA) is usually used for blocking the nonspecific reaction even thought it is expensive. Recently skin milk has been focused on blocking nonspecific reaction of ELISA. This study aimed to substitute expensive blocking agent of ELISA with cheap by-product of cheese manufacture. To evaluate the substitution of BSA with wheys made in different companies, ELISA was carried out using monoclonal antibodies (MAbs) against rotavirus. The blocking capacity of the whey was compared with that of the BSA. The commercial wheys showed the same activity as BSA when ELISA was performed using MAb YO-156, S2-37, and Yo-5. To further investigate whether the whey affects the detection specificity of MAb, the ELISA was carried out using type specific MAbs. MAb G6 specific to serotype G6 bound to only rotavirus serotype G6 such as NCDV, UK, JBR, and Jedong strain whereas MAb G10 specific to serotype G10 bound to serotype G10 rotavirus only, demonstrating that the whey a and no effect on the binding specificity of MAb with respect to antigen-antibody reaction. These data indicate that the whey may have no effect on specific interation of antigen-antibody and may be widely used for blocking substitute because of competitive priority of its price.