택사추출액이 benzo(a)pyrene에 의해 유도된 간장해에 미치는 영향

尹水弘,徐旼志,河勳 대구효성가톨릭대학교 1996 연구논문집 Vol.52 No.2

The protective and therapeutic effects of Alismae Rhizome extracts depending on solvent polarity on rat liver injury induced by benzo(a)pyrene were investigated. As parameters in serum and liver, alkaline phosphatase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities were observed. The results were as follows: B(a)P intoxication significantly increased alkaline phosphatase, transaminase activities of serum and liver compared with normal group. Especially CHCI₃fraction revealed the most powerful liver protective and/or therapeutic effect of 4 extracts in serum transaminase activities and in hepatic alkaline phosphatase and glutamate oxaloacetate transaminase, maybe due to presence of alkaloid, triterpenoid and flavonoid in this fraction. Above-mentioned results strongly suggested that Alismae Rhizoma be a promising liver-protective drug. So more study will be needed to find principle hepatotropic Alismatis Rhizoma.

Benzo(a) pyrene에 의해 유도된 간장해에 미치는 황련 수침액의 영향

윤수홍,하헌,서민지 한국환경독성학회 1993 환경독성보건학회지 Vol.8 No.1

The present study was undertaken to find a protective effect of Coptis Rhizoma Extract on liver injury induced by benzo (a)pyrene in rats. This crude drug significantly suppressed the increase of several biochemical parameters such as transaminase, lactate dehydrogenase and alkaline phosphatase in serum and liver and total cholesterol. But there were little changes in elevated serum phospholipid level. The results suggested that Coptis Rhizoma prevent bezo (a)pyrene-induced hepatic injury.

열대 지역에서 통일형 초다수성 벼 품종의 생육 및 수량성 비교

이지윤,준현,성환,손영보,송유천,한상익,박동수,권영업,이철원,이종희 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

대전광역시 대전천 물로부터 분리한 고온성, 호염성 야생효모, Pichia spartinae WJSL0087의 생육 특성

김지윤, 한상민, 이종수 배재대학교 자연과학연구소 2017 自然科學論文集 Vol.28 No.1

대전광역시 대전천 주변의 물에서 분리한 고온성이며 호염성인 Pichia spartinae WJSL0087의 생육특성을 조사하였다. Pichia spartinae WJSL0087는 yeast extract-peptone-dextrose 배지에서 생육이 양호하였고 37℃와 15% NaCl 함유 YPD 배지에서 생육하는 고온성, 호염성 효모이었다. 또한, Pichia spartinae WJSL0087는 포도당 30%, 10% 에탄올 함유 배지에서도 생육하였고 2가의 Co 와 Cu, 1가의 리튬 양이온들에 대하여 500ppm까지, NaCl, KCl등은 15%까지 내성을 보였다. The goal of this study was to investigate growth characteristics of the thermpphilic and halotolerant wild yeast, Pichia spartinae WJSL0087 from freshwater of riverside in Daejeoncheon, Daejeon Metropolitan city, Korea. Pichia spartinae WJSL0087 grew yeast extract-peptone-dextrose (YPD) medium and also grew in 15% NaCl-YPD medium at 37℃. The strain were resistrant to 30% glucose, 10% ethanol, !5% KCl and CaCl2 and further, also grew in the 500 ppm Co, Cu and Li metal ions-containing YPD medium, respectively.

일부 산업장 근로자들의 직업성 피부질환 및 천식의 유병조사

윤지석,이영수,조영채,이동배 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

For the analysis of prevalence of occupational skin diseases and asthma in industrial workers, we studied the 1,274 workers who were engaged in manufacturing workers(direct exposured group to harzardous materials) and clerical workers (indirect exposured group to harzardous materials). The results were as follows: 1. The prevalence rate of occupational skin diseases and asthma in manufacturing workers(36.4%) was significantly higher than that in clerical workers(25.2%) (p<0.005). 2. The prevalence rates of skin diseases(p<0.005) and asthma(p<0.05) in male manufacturing workers were significantly higher than those in clerical workers, and the prevalence rate of asthma(p<0.005) in female manufacturing workers was significantly higher than that in clerical workers. 3. Of asthma in twenties(p<0.005), of skin diseases(p<0.005) and asthma(p<0.05) in thirties, the prevalance rate of manufaturing workers were higher than thoses of clerical workers, however no diffrence was in forties. 4. Of skin diseases(p<0.005) and asthma(p<0.05) in metal plating, of asthma(p<0.005) in cotton spinning, the prevalence rate of manufacturing workers were significantly higher than those of clerical workers. 5. The prevalence rate of skin diseases and asthma in dustness part were significantly higher than those of non-dustness part and poor ventilation part also were significantly higher than good ventilation part (p<0.005). 6. Frequency of involved skin sites, in descending order, were hand(32.1%), leg(24.3%), trunk(20.4%), face(12.1%) and arm(11.1%).

레트로바이러스의 표면단백질 : 인슐린의존형 당뇨병의 동물모델인 NOD 쥐의 혈청과 반응하는 새로운 자가항원 A New Autoantigen Reactive with Non Obese Diabetic (NOD) Mice Sera

김경수,김기환,김현만,윤지원,강엽 아주대학교 1997 아주의학 Vol.2 No.2

IDDM (Insulin dependent diabetes mellitus) is believed to be an autoimmune disease and characterized by the immune activation against insulin-producing pancreatic beta cell. The identification and characterization of new autoantigens reactive with an activated immune System would heip to elucidate the pathogenic mechanism of this disease. Several autoantigens are trying to apply for diagnosis and prevention of IDDM. The NOD (non obese diabetic) mice have been the best model for studying the pathogenesis of human IDDM. To identify new autoantigens reactive with activated humoral immunity of NOD mice, the lambda gt11 cDNA library was constructed from NOD-derived pancreatic beta cell (MIN6N8a: mouse insulinoma cell) and screened with prediabetic NOD sera. Mine positive dones were selected from 2x10^(5) phage plaques. The 5'-end sequencing and homology searching showed that six clones from nine clones had over 98% sequence homolgy with the retroviral envelope gene. Full sequendng reveated that the cloned gene was a fragment of ecotropic retrovirus (emv-3) envelope gene. To confirm the immunoreactivity of doned retroviral envelope protein, the cloned gene fragment was expressed in an E.coli expression vector System. Western blotting showed that the recombinant envelope protein fragment also reacted with prediabetic NOD sera.

한국산 큰가시고기과 어류의 형태변이와 핵형

김익수,윤창호,이지현 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.1

우리나라 연안과 담수역에 서식하는 큰가시고기과(Gasterosteidae)어류의 많은 표본은 1988년 3월부터 1989년 6월까지 채집하여 이들의 형태변이와 분류학적 위치에 대하여 검토하였다. 큰가시고기 Gasterosteus aculeatus 6개 조사집단의 체측인판수는 모두 32-35개였고 그 형태는 완판형으로 국내에서는 아직 육봉형이라고 생각되는 저판형이나 반판형은 발견되지 않았다. G. aculeatus의 척추골수, 세파수, 지느러미 기조수 등의 계수형질이나, 두장, 안경 및 등지느러미가시길이의 계측치는 집단간에 뚜렷한 차이가 없었다. 가시고기 Pungitius sinensis와 잔가시고기 P. Kaibarae ssp. 집단의 체측인판수는 모두 31-35개의 완판형이었고, 등지느러미가시 기조수, 척추골수 및 체고비등에 잇어서 2종간에 구별되었지만, 잔가시고기 P. Kaibarae ssp.의 집단에서는 등지느러미가시 기조막에 담회색을 딘 표본(white form)이 출현하였는데 이것은 이들이 서식하는 환경과 관련된 변이라고 본다. 이 2종의 염색체의 수는 모두 2N=42로서 같지만 G. aculeatus의 핵형은 metacentrics이 6개, submetacentrics이 6개 subtelo-acrocentrics이 30개이고, P. sinensis와 P. Kaibarae ssp.는 모두 metacentrics이 4개, submetacentrics이 4개 subtelo-acrocentrics이 34개로 2속 사이에는 서로 달랐다. Three species of Korean sticklebacks family Gasterosteidae were reviewed based on the specimens of several populations for the study of their morphological variations and taxonomical positions. All specimens of Gasterosteus aculeatus observed have a complete row of lateral plates ranging from thirty-two to thirty-five in number. The low-plate morph regarded as landlocked type is virtually absent. And no difference was recognized among six population of G.aculeatus in the meristic characters, i.e. number of vertebrae, gill rakers and fin rays. the Pungitius sinensis and P. Kaibarae ssp. shown local variations have scutes ranging from 31 to 35, but not clinal in the morphometric characters. But P.sinensis was statistically different from P.kaibarae ssp. in some characters such as the number of dorsal spine rays and vertebrae, and body depth, although their ranges overlapped. No significant taxonomic difference was detected between white form and black form in dorsal spinous membrane of P.Kaibarae ssp. Although these three species of Korean sticklebacks have the same diploid chromosome number(2N=42), G. aculeatus is obviously different from the two species of genus Pungitius in their karyotype; G. aculeatus consists of six metacentrics, six submetacentrics, and thirty subtelo-acrocentrics, and both P. sinensis and P. kaibarae ssp. have four metacentrics, six submetacentrics, and thirty-four subtelo-acrocentrics.

에탄올성 간손상 흰쥐의 유해산소 생성효소계에 미치는 칡 추출물의 영향

조수열,김명주,박은미,박지윤,장주연 한국노화학회 2001 한국노화학회지 Vol.11 No.1

B16 melanoma 세포에서 nitric oxide가 멜라닌 생성에 미치는 영향

심상수,조남영,윤미연,이지윤,김창종 중앙대학교 약학연구소 2002 약학 논총 Vol.16 No.-

To investigate the effects of nitric oxide and cGMP on melanin production, we measured the melanin production in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH). NO donor, such as, sodium nitroprusside (SNP), SIN-1, SNAP and L-arginine increased melanin production in basal state. SNAP potentiated melanin production by MSH, but other NO donors did not. In the study the potentiating effect of sodium nitroprusside on cAMP-dependent melanin production, sodium nitroprusside did not increase melanin production induced by MSH, forskolin and 8-Br-cAMP. Non-selective cAMP/cGMP phosphodiesterase inhibitor (IBMX) and selective cGMP phosphodiesterase inhibitor (zaprinast) significantly increased MSH-induced melanin production, whereas soluble guanylyl cyclase inhibitor (ODQ) inhibited MSH-induced melanin production. These results suggest the possibility that MSH-stimulated melanin production may be mediated via not only cAMP-dependent pathway but also cGMP-dependent pathway in B16 melanoma cells.

중·고등학교 급식종사자들의 위생관련 업무 수행도 분석

홍완수,윤지영 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.4

The purpose of this study was to develop methods for foodservice employees to perform by measuring their levels of sanitation and hygiene practices. The employees' sanitation and hygiene competency list for school foodservice was developed on the basis of the job standardization. The competency list was divided into three parts; (1) before starting the work, (2) during the work and (3) after the work. The levels of the employees' sanitation and hygiene practices were evaluated by dietitians and by the employees themselves. Most schools had conventional foodservice systems (83.4%), which were operated by contract management (94.8%). It was found that the highest practice level related to sanitation and hygiene before starting work, with the lowest levels observed after work. The item related to the cleaning and sanitizing of dishes had the lowest practice level score. Employees perceived their sanitation and hygiene practice after work to be worse than before starting and during work. The items of "Clean and sanitize all large stationary equipment after every use, and record equipment monitoring chart" and "Do not clean dishes and utensils in production area" had the lowest scores by employees. The scores of the employees were similar to the perception of the dietitians.