Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells

Yang, Ha-Na,Lee, Seung-Eun,Jeong, Seong-Il,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong-Seek The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.3

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on $H_2O_2$-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells

Hana Yang,Seung Eun Lee,Seong Il Jeong,Cheung-Seog Park,Young-Ho Jin,Yong Seek Park 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on H₂O₂-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression

Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7

<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>

Crotonaldehyde-exposed macrophages induce heme oxygenase-1 expression as an adaptive mechanism

Seung Eun Lee,Hana Yang,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Cigarette smoke represents one of the most significant risk factors for the progression of vascular disease. Exposure to cigarette smoke induces oxidative stress in the vascular system and results in vascular dysfunction. Crotonaldehyde, a highly reactive α,β-unsaturated aldehyde, is a foremost compound of cigarette smoke and a product of endogenous lipid peroxidation. Heme oxygenase-1 (HO-1) expression plays an essential role in cellular defense against environmental insults and represents an adaptive response. Here, we showed the effects of crotonaldehyde on the induction of HO-1 expression in RAW 264.7 macrophages. Crotonaldehyde treatment resulted in significantly increased phosphorylation of p38 mitogenactivated protein kinase (MAPK) and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, treatment with zinc protoporphyrin (ZnPP; the specific HO-1 inhibitor) markedly augmented the death of crotonaldehyde-treated macrophages. In summary, these results highlight the role of the HO-1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response to crotonaldehyde in macrophages.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

고추 탄저병 저항성 중간모본 ‘원예 100005호 ’,‘원예 100006호’ 육성

양은영,김수,채영,채수영,조명철,문지혜,박태성,이우문,곽정호 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Owing to frequent heavy rain due to abnormal weather, the occurrence of pepper anthracnose has recently increased, which affects the supply and demand of pepper. As a result, the price of red pepper powder in 2011, for example, increased three times compared to the average year. Although anthracnose of pepper plants has been controlled by fungicides until now, it is important to develop new resistant cultivars because of the reduction of the labor force in rural area and occurrence of new anthracnose strains resistant to fungicides. National Institute of Horticultural and Herbal Science developed two pepper lines, ‘Wonye 100005’ and ‘Wonye 100006’ that are resistant to pepper anthracnose. These lines were developed by continuous selection of progenies from a cross between ‘Wonye AR1’, which were developed from interspecific hybridization and ‘TF68’. The anthracnose resistance level of the two lines was higher than that of commercial cultivars and similar to check varieties showing highest level of anthracnose resistance.

수종 치과용 광중합형 복합레진의 화학적 분해

양규호,최남기,박미란,박은혜 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

이상적인 수복재료의 요건으로 치아의 저작기능과 심미성을 충분히 회복시킬 수 있는 물리적. 화학적 성질을 갖추는 동시에 생물학적인 적합성과 구강내 환경변화에 따른 내구성 등을 들 수 있다 그러나 복합레진을 이용하여 치아를 수복하였을 때 수복물은 구강내에서 시간이 흐름에 따라 타액내에서 서서히 분해되고 교합력이나 음식물에 의해 마모되어가는 운명을 거친다. 본 연구에서는 현재 수복재료로서 많이 사용되고 있는 Definite(Degussa-Hiils AG. Germany), Revolution(Kerr, U.S.A.) Unifil(GC, Japan) , Palfique(Tokuyama, Japan)의 4종의 복합레진을 알카리성 용액 (0. IN NaOH)에 보관하였을 때 각 제품의 분해저항성을 평가하고자 하였다. 각 제품당 3개의 시편을 제작하여 무게측정을 한 후 0.IN NaOH에 저장하여 60℃에서 보관하였다. 2주 후 제거하여 HCI로 중화, 세척 후 60℃에서 건조하였다 무게손실, 표면하분해층 깊이, 용출된 Si농도를 기준으로 각 레진의 분해저항성을 평가하여 다음과 같은 결과를 얻었다. 1. 무게손실량은 Unifil에서 3.21%로 가장 높았으나 각각 제품간에 있어서의 차이를 보이지는 않았다. 2. 분해층 깊이는 107.69∼47.407n의 범위였고, Unifil, Palfique. Revolution, Definite 순으로 감소하였으며 Palfique. Definite를 제외한 다른 제품간에는 유의한 차이를 보였다(p(0.05). 3. Si 용출량은 Palfique가 8940.Oppm으로 가장 많은 용출량을 보였으며 Revolution과 Definite를 제외한 각 제품간에는 유의한 차이를 보였다(p<0.05). 4. 각 제품의 무게손실과 분해층 깊이 사이에는 높은 상관관계를 보였다(r= 0.06, p<0.05) 5. 각 제품의 무게손실과 Si 용출량, 분해층 깊이와 Si 용출량 사이 에는 유의한 상관관계를 보이지 않았다. 6. 주사전자현미경 관찰시 NaOH용액에 보관한 후 레진 기질과 필러 사이의 결합의 파괴를 관찰할 수 있었다. The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. The brands studied were Unifil(GC, Japan) , Palfique(Tokuyama, Japan) , Definite(Degussa-Huls AG, Germany) . Revolution(Kerr U.S.A.) . Preweighed discs of each brand were exposed 0. IN NaOH solution at 60℃. After 14 days they were removed, neutralized with HCI, washed with water and dried. Resistance to degra-dation was evaluated on the basis of following parameters : (a) mass loss(%) - determined from pre-and post-ex-posed specimen weights: (b) Si loss(ppm) - obtained from ICP-AE analysis of solution exposed to specimens and (c) degradation depth(㎛) - measured microscopically (SEM) from polished circular sections of exposed specimens. The results were follows. 1 The mass loss of Unifil was 3.21%, it was the highest of materials. But, there was no significant difference among the materials. 2. The degree of degradation layer depth was 107.69∼47.40㎛, the sequence of the degree pf degradation layer depth was in descending order by Unifil, Palfique, Revolution Definitre. There was significant difference among the materials except Palfique and Definite. 3. The Si loss of Paltique was 8940.Oppm, it was the highest. There was significant difference among the materials, except Revolution and Definite(p<0.05). 4. The correlation coefficient between mass loss and degradation depth was relatively high(r = 0.06. p<0.05). 5. There was no significant coefficient correlation between Si loss and mass loss, and/or the degree of degradation layer depth and Si loss. 6. When observed with SEM, destruction of bonding is observed between resin matrix and filler. Above results suggested that the hydrolytic degradation is considered as evaluation factor of composite resins.

조기 양막 파수 신생아에 대한 임상적 연구

양은석,김강호,박상기,나창수 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.1

A clinical study was made on 157 newborns of mothers with premature rupture of membranes (study group) and 916 newborns of mothers without rupture of membranes (control group) at Chosun University Hospital from Jaunary 1987 to Decembcr 1988. The results were as follows: 1) The incidence of PROM was 14.6%. 2) 10.2% of all babies with PROM were born prematurely, significantly higher than the incidence of PROM in all newborn cases with 4.8% (P<0.005). 3) The incidence of neonatal sepsis in the PROM group (4.5%) was four times higher than in the normal group(1.1%) (P<0.005). The incidience was related to the gestational age at birth, and also increased with increasing time interval after PROM. 4) The incidence of neonatal asphyxia in PROM group (7.6%) was twice the normal group (3.6%) (P<0.025). The premature babies had greater incidence in both groups. Also, a positive correlation was found with increasing time interval PROM. 5) Neonatal respiratory distress syndrome(RDS) was found in 1.3% of babies with PROM, whereas 1.4% of babies in the normal group had the syndrome. Premature babies had higher incidence of RDS in both normal and PROM groups, but the latter had an incidence amounting the only 1/5 of the former. And it tended to diminish with creasing time interval after PROM.

전국 가정의학과 전공의 수련내용

양병국,이행,윤영호,조은연,유태우,허봉렬 大韓家庭醫學會 1993 Korean Journal of Family Medicine Vol.14 No.4-5

차세대 레진 개발을 위한 광중합형 복합레진의 화학적 분해와 마모에 관한 연구

양규호,최은영,최남기,김선미 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.3

전남대학교 치과대학 소아치과학교실 및 치의학 연구소 수복재료로 사용되는 복합레진은 치아의 저작기능과 심미성을 회복할 수 있는 물리적, 화학적 성질뿐만 아니라 생물학적 적합성과 구강내 환경변화에 대한 내구성을 가지고 있어야 한다. 유치에 사용하려고 할 때에도 마모저항성과 구강내 환경에서의 분해저항성은 고려해야할 중요한 물성이다. 실험에 사용된 복합레진은 최근에 시판되고 있는 Metafil CX(Sun Medical, Japan) Solitaire 2(Heraeus Kulzer, USA), Composan LCM(Promedica, Germany), DenFil(Vericom, Korea)이었다. 각 제품의 분해저항성과 마모도를 평가하고자 마모시험 후 마모된 면의 깊이를 측정하였고 화학적분해를 얻기 위해 0.1N NaOH에 보관 시 각 제품의 분해저항성을 무게손실, 표면하 분해층 깊이, 용출된 Si 농도를 기준으로 평가하였고 주사전자현미경과 공촛점 레이저 현미경으로 분해층을 관찰하여 다음과 같은 결과를 얻었다. 1. 무게 손실량은 0.74~7.94%까지 다양하였으며 Metafil에서 가장 높았다. 2. 분해층 깊이는 Metafil이 가장 깊었고 Solitare 2, Denfil, Composan LCM순이었고 Metafil은 다른제품과 유의한 차이를 보였다(p<0.05). 3. Si 용출량은 Metafil이 가장 많았으며 Metafil CX와 Composan LCM, DenFil, Solitaire 2사이에 유의한 차이를 보였다(P<0.05). 4. 주사전자현미경 관찰시 표면 양상 및 분해층 깊이를 관찰할 수 있었고 공촛점 레이저 현미경 관찰시 NaOH용액에 보관한 후 수복재의 기질과 충전제 사이의 결합의 파괴 양상을 관찰할 수 있었다. 5. 최대마모깊이는 DenFil에서 가장 낮았고, Metafil CX에서 가장 깊었으며 각 제품 간에는 유의한 차이를 보였다(p<0.05). 6. 각 제품의 Si 용출량과 분해층 깊이 사이 (r=0.491, p<0.05), 최대마모깊이와 비커스경도 간(r=-0.942, p<0.01)에 유의한 상관관계를 보였다. 이상의 결과 복합레진의 평가요소로서 마모도와 함께 가수분해도 고려되어야 할 것으로 사료된다. The aim of this study was to evaluate the resistance to degradation and to compare the wear resistance characteristics of four esthetic restorative materials in an alkaline solution(0.1N NaOH). The brands studied were MetafilCX(Sun medical, Japan) Solitaire 2(Heraeus Kulzer, USA), Composan LCM(Promedica, Germany), DenFil(Vericom, Korea). The results were as follows: 1. The mass loss of each brand was 0.74~7.94% and highest value in Metafil(7.94±0.39%). 2. The sequence of the degree of degradation layer depth was in descending order by Metafil, Solitaire 2, DenFil, Composan LCM. There were significant differences between Metafil and the others(p<0.05). 3. The sequence of the Si loss was in descending order by Metafil, Solitaire 2, Composan LCM, DenFil. There were significant differences among the materials(p<0.05). 4. On SEM, destruction of bonding between matrix and filler and on CLSM, the depth of degradation layer of specimen surface was observed. 5. The sequence of maximum wear depth was in descending order by Metafil, Solitaire 2, Composan LCM and DenFil. There were significant differences among the materials(p<0.05). 6. The correlation coefficient between Si loss and degradation layer depth (r=0.491, p<0.05) and Vicker's hardness number and maximum wear depth (r=-0.942, p<0.05) were relatively high. These results indicate that hydrolytic degradation and wear may consider as a evaluation factors of composite resins.