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Profiling of miRNA expression in mice kidney with diabetic nephropathy
Park, Hye Rim,Lee, Seung Eun,Kim, Hyemi,Jeon, Seeun,Han, Dongkyo,Jin, Young-Ho,Cho, Jeong-Je,Ahn, Hyun-Jong,Park, Cheung-Seog,Lee, Jongsung,Park, Yong Seek THE KOREAN SOCIETY OF TOXICOGENOMICS AND TOXICOPRP 2018 MOLECULAR AND CELLULAR TOXICOLOGY Vol. No.
The Purification and Characterization of Bacillus subtilis Tripeptidase (PepT)
Park, Yong-Seek,Cha, Myung-Hoon,Yong, Whan-Mi,Kim, Hyo-Joon,Chung, Il-Yup,Lee, Young-Seek Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.3
A tripeptidase (PepT) was purified to homogeneity from Bacillus subtilis through four sequential chromatographies including DEAE-Sepharose ion exchange, hydroxylapatite, mono-Q FPLC ion exchange, and Superose-12 FPLC gel filtration. The apparent molecular mass of the enzyme was 49,200 Da and 51,400 Da as determined by sodium dodecylsulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography, respectively, and the enzyme exists in a monomeric form. The physicochemical properties of the enzyme were as follows: optimum pH at 7.5, optimum temperature at $60^{\circ}C$, and pI at 4.9. The $K_m$ and $V_{max}$ values of the enzyme were 4.3 mM and 2.5 mmol/min/mg, respectively, with MetAla-Ser as substrate. The B. subtilis PepT requires $Co^{2+}$ ion(s) for activation, while it is inactivated by EOTA and 1,10-phenanthroline, suggesting that it is a metalloprotein. The enzyme was not inhibited by any of serine protease, aspartic protease, or leucine aminopeptidase inhibitors. The enzyme showed comparable activities towards four different substrates including Met-Ala-Ser, Leu-Gly-Gly, Leu-Ser-Phe, and Leu-Leu-Tyr. The amino terminal sequence of PepT determined by Edman degradation was found to be MKEEIIERFTTYVXV and turned out to be identical to that of PepT deduced from a cloned B. subtilis pepT.
Yong Seek Park,이승은 셀메드 세포교정의약학회 2013 TANG Vol.3 No.1
The present review investigates the role of the cytoprotective enzyme heme oxygenase-1 (HO-1) in human diseases and explores strategies for its clinical use. In recent years, there has been a growing evidence, for the beneficial effects of some phytoconstituents via induction of HO-1 expression, contained in commonly used spices, fruits, and herbs, in preventing various pathologic conditions, including cancer, diabetes, and cardiovascular diseases. HO-1 catalyzes the rate-limiting step in heme catabolism to generate ferrous iron, carbon monoxide, and biliverdin. HO-1 is reported to play crucial roles in cellular protection, such as anti-inflammatory, anti-proliferative and anti-apoptotic effects. These evidences indicate that HO-1 may functions as a potential therapeutic target in various human diseases. The article highlights the current status of the development of the HO-1 modulation pathway using dietary phytoconstituents.
박용규 ( Park Yong Kyu ),윤주용 ( Yoon Ju Yong ),최병걸 ( Choi Byung Keol ),김태익 ( Kim Tae Ik ),이대식 ( Lee Dae Seek ) 한국구조물진단유지관리공학회 2022 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.26 No.1
콘크리트 체적에 가장 많은 부분을 차지하는 골재는 최근 골재자원의 고갈 및 환경 규제강화 등으로 수급상황이 어려워지고 있는 상황이다. 특히 골재 업체에서는 이윤추구를 목적으로 채취시 골재에 포함된 토분을 제거하지 않은 채 시중에 유통되고 있는 실정이다. 이에 본 연구에서는 메틸렌블루 시험방법을 이용하여 잔골재를 분류하고, 콘크리트 물성 평가를 통하여 비교 분석하고자 하였다. 실험결과 토분이 함유된 잔골재를 사용할 경우 단위수량이 증가하고 공기량이 저하하여 콘크리트 품질저하를 야기하는 것으로 나타났다.