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Profiling of miRNA expression in mice kidney with diabetic nephropathy
Park, Hye Rim,Lee, Seung Eun,Kim, Hyemi,Jeon, Seeun,Han, Dongkyo,Jin, Young-Ho,Cho, Jeong-Je,Ahn, Hyun-Jong,Park, Cheung-Seog,Lee, Jongsung,Park, Yong Seek THE KOREAN SOCIETY OF TOXICOGENOMICS AND TOXICOPRP 2018 MOLECULAR AND CELLULAR TOXICOLOGY Vol. No.
Selective GSK-3β inhibitors attenuate the cisplatin-induced cytotoxicity of auditory cells
Park, Hee-Je,Kim, Hyung-Jin,Bae, Gi-Sang,Seo, Sang-Wan,Kim, Do-Yun,Jung, Won-Seok,Kim, Min-Sun,Song, Mi-Young,Kim, Eun-Kyung,Kwon, Kang-Beom,Hwang, Sung-Yeon,Song, Ho-Joon,Park, Cheung-Seog,Park, Rae- Elsevier 2009 Hearing research Vol.257 No.1
<P><B>Abstract</B></P><P>Glycogen synthase kinase-3 (GSK-3) plays an important role in the regulation of apoptosis. However, the role of GSK-3 in the auditory system remains unknown. Here we examined whether the GSK-3-specific inhibitors, SB 216763 and LiCl, could protect against cisplatin-induced cytotoxicity of auditory cells. GSK-3 was activated by cisplatin treatment of HEI-OC1 cells. SB 216763 or LiCl treatments inhibited cisplatin-induced apoptosis in a dose-dependent manner and activated caspase-9, -8 and -3. In rat primary explants of the organ of Corti, SB 216763 or LiCl treatments completely abrogated the cisplatin-induced destruction of outer hair cell arrays. Administration of SB 216763 or LiCl inhibited cochlear destruction and the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in cisplatin-injected mice. Furthermore, administration of SB 216763 or LiCl reduced the thresholds of the auditory brainstem response (ABR) in cisplatin-injected mice. Collectively, these results suggest that cisplatin-induced ototoxicity might be associated with modulation of GSK-3 activation.</P>
Effect of crotonaldehyde on the induction of HO-1 expression in A549 cells
Seung Eun Lee,Hye Rim Park,Hong Duck Yun,Hyemi Kim,진영호,Cheung-Seog Park,Hyun-Jong Ahn,JeongJeCho,Yong Seek Park,Y. S. Park 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.2
Cigarette smoke contains approximately 5,000 chemical components, including reactive chemicals and free radicals that are associated with an increased risk of chronic obstructive pulmonary disease (COPD). Cigarette smoke is also known to promote inflammation as part of various inflammatory airway diseases. Crotonaldehyde (CRA) is a highly toxic α, β-unsaturated aldehyde that is a major component of cigarette smoke. Exposure to CRA has been previously shown to result in adverse effects on respiratory health. Heme oxygenase-1 (HO-1) is an inducible enzyme that is activated by various stress-inducing stimuli to perform a diverse range of physiological functions, including anti-oxidant, anti-apoptotic, and anti-inflammatory effects. The present study aimed to investigate the effect of CRA stimulation on HO-1 expression in human lung adenocarcinoma epithelial (A549) cells. Stimulation of cells with CRA was shown to result in upregulated HO-1 expression via the induction of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation, and activation of the p38 mitogen-activated protein kinase (MAPK) pathway. Furthermore, zinc protoporphyrin (ZnPP; a specific HO-1 inhibitor) was used to inhibit HO-1 activity, and this was shown to cause a significant increase in the rate of apoptosis of CRAexposed cells. Taken together, these results suggest that HO-1 exerts an anti-apoptotic effect in CRA-exposed human lung adenocarcinoma epithelial cells, and thus protects cells against CRA-induced oxidative stress.
Effect of crotonaldehyde on the induction of COX-2 expression in human endothelial cells
Seung Eun Lee,Hye Rim Park,Hyemi Kim,Yeoum Choi,Young-Ho Jin,Cheung-Seog Park,Hyun-Jong Ahn,Jeong-Je Cho,Yong Seek Park,Y. S. Park 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.3
Cyclooxygenase-2 (COX-2), an inducible isoform protein, regulates diverse biological actions in vascular pathophysiology. COX-2 is induced in response to numerous stimuli, which results in prostaglandin (PG) production related to inflammation. Crotonaldehyde (CRA) is an extremely toxic α, β-unsaturated aldehyde and a major compound found in cigarette smoke. α, β-Unsaturated aldehyde in cigarette smoke is thought to mediate inflammation and vascular dysfunction. In this study, we evaluated the effect of CRA stimulation on COX-2 expression in human umbilical vein endothelial cells. CRA-stimulated COX-2 induction was accompanied by enhanced p38 phosphorylation and PGE2 generation. However, CRA-induced PGE2 production was reduced by pretreatment with an inhibitor of p38 MAPK. These results demonstrated that in human endothelial cells, CRA-induced COX-2-dependent PGE2 generation was mediated by p38 MAPK, and CRA may play a role in the development of inflammation.
Seung Eun Lee,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.4
Cigarette smoking is one of the main sources of toxic chemical exposure to humans and is the greatest cause of progression of vascular disease. Crotonaldehyde is one of the major constituents of cigarette smoke and a product of endogenous lipid peroxidation. Thioredoxin reductase (TrxR) is a key element of the intact thioredoxin (Trx) system, which is predominant in modulating the intracellular redox homeostasis. In this study, we showed the effects of crotonaldehyde on the induction of TrxR1 expression in human endothelial cells. Crotonaldehyde exposure caused notably increased phosphorylation of p38 mitogen-activated protein kinase (MAPK). Introduction of siRNA against nuclear factor erythroid 2-related factor 2 (Nrf2) downregulated TrxR1 expression. Furthermore, treatment with auranofin, a TrxR inhibitor, significantly increased the death rate of crotonaldehyde-exposed cells. In summary, these results suggest a role for TrxR1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response of human endothelial cells to crotonaldehyde.
Alpha-lipoic acid protects against cholecystokinin-induced acute pancreatitis in rats.
Park, Sung-Joo,Seo, Sang-Wan,Choi, Ok-Sun,Park, Cheung-Seog WJG Press 2005 WORLD JOURNAL OF GASTROENTEROLOGY Vol.11 No.31
<P>Alpha-lipoic acid (ALA) has been used as an antioxidant. The aim of this study was to investigate the effect of alpha-lipoic acid on cholecystokinin (CCK)-octapeptide induced acute pancreatitis in rats.</P>
Park, Jun Seok,Sakai, Yoshiharu,Simon, NG Siu Man,Law, Wai Lun,Kim, Hyeong Rok,Oh, Jae Hwan,Shan, Hester Cheung Yui,Kwak, Sang Gyu,Choi, Gyu-Seog Wolters Kluwer Health, Inc. All rights reserved. 2016 Medicine Vol.95 No.22
<P>Controversy remains regarding whether preoperative chemoradiation protocol should be applied uniformly to all rectal cancer patients regardless of tumor height. This pooled analysis was designed to evaluate whether preoperative chemoradiation can be safely omitted in higher rectal cancer. An international consortium of 7 institutions was established. A review of the database that was collected from January 2004 to May 2008 identified a series of 2102 patients with stage II/III rectal or sigmoid cancer (control arm) without concurrent chemoradiation. Data regarding patient demographics, recurrence pattern, and oncological outcomes were analyzed. The primary end point was the 5-year local recurrence rate. The local relapse rate of the sigmoid colon cancer (SC) and upper rectal cancer (UR) cohorts was significantly lower than that of the mid/low rectal cancer group (M-LR), with 5-year estimates of 2.5% for the SC group, 3.5% for the UR group, and 11.1% for the M-LR group, respectively. A multivariate analysis showed that tumor depth, nodal metastasis, venous invasion, and lower tumor level were strongly associated with local recurrence. The cumulative incidence rate of local failure was 90.6%, 92.5%, and 94.4% for tumors located within 5, 7, and 9 cm from the anal verge, respectively. Routine use of preoperative chemoradiation for stage II/III rectal tumors located more than 8 to 9 cm above the anal verge would be excessive. The integration of a more individualized approach focused on systemic control is warranted to improve survival in patients with upper rectal cancer.</P>
Yang, In Seog,Park, Cheung Yeoul,Kim, Soo Gwan 朝鮮大學校 口腔生物學硏究所 2000 口腔生物學硏究 Vol.24 No.1
본 연구는 두개골에 20㎜ 크기의 전층골결손을 형성한 후 자가망상골과 냉동건조탈회골을 용적비를 달리하여 이식하여 조직학적으로 평가하여 골 형성을 알아보는 데 그 목적이 있다. 조직학적인 검사와 조직형태계측학적 분석 및 통계학적 분석을 수술후 12주와 24주에 시행하였다. 16마리의 성견을 4군으로 구분하여 1군은 이식을 시행하지 않은 대조군, 2군은 자가망상골과 냉동건조탈회골을 4:1로 혼합하여 이식한 군, 3군은 자가망상골과 냉동건조탈회골을 1:1로 혼합하여 이식한 군, 4군은 자가망상골과 냉동건조탈회골을 1:4로 혼합하여 이식한 군으로 구분하였다. 조직학적인 검사와 조직형태계측학적 분석 및 통계학적 분석을 시행한 결과, 2군인 자가망상골과 냉동건조탈회골을 4:1로 혼합하여 이식한 군에서 가장 좋은 결과를 보였으며, 자가망상골과 냉동건조탈회골을 1:1로 혼합하여 이식한 군도 이와 비슷한 결과를 보였다. 이식을 시행하지 않은 대조군에서는 가장 좋지 않은 결과를 보였다. 이러한 결과는 자가망상골의 양이 많을수록 이식후 골성장이 많아지며 비록 자가골의 양이 적을지라도 골형성은 되었지만, 이식술이 필요한 경우에는 자가망상골의 양이 50% 이상 이식을 시행하므로써 최대한의 이식 효과를 얻을 수 있으리라 사료되었다.
Genome-wide analysis of gene expression by crotonaldehyde in human umbilical vein endothelial cells
Seong Il Jeong,이승은,양하나,Cheung-Seog Park,조정제,Yong Seek Park 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.2
Crotonaldehyde (CRA) is an α,β-unsaturated aldehyde that is genotoxic, carcinogenic, and mutagenic, and forms 1,N(2)-propanodeoxyguanosine. Humans are exposed to CRA in work places, and from tobacco smoke, environmental pollution, food, and beverages. In addition, CRA is a product of endogenous lipid peroxidation, arising as a consequence of oxidative stress and electrophilic stress, which may be related to development of cardiovascular diseases. In this study, we used 24 k whole human DNA chips for identification of gene expression profiles by treatment of CRA in human umbilical vein endothelial cells (HUVECs). Among the 24,000 genes of cDNA microarrays, we identified up- and down-regulated genes that changed by more than 1.8 and 2 fold by CRA. Our data showed that CRA changed gene expression patterns at the genome-wide transcriptional level in HUVECs. And several genes showed association with cardiovascular diseases.