BMP test를 통한 음폐수와 하수슬러지의 병합소화 특성 평가

이수영ㆍ윤영삼ㆍ강준구ㆍ김기헌ㆍ신선경(Su young LeeㆍYoung Sam YoonㆍJun Gu KangㆍKi Heon KimㆍSun Kyoung Shin) 유기성자원학회 2016 유기물자원화 Vol.24 No.1

혐기성 소화의 주요 조건 중 하나인 C/N비의 경우 하수슬러지는 5.40으로 낮게 나타난 반면 음폐수(Food waste leachate)는 21.84로 높게 나타났다. C/N비가 낮을 경우 혐기성소화의 저해 요인으로 작용될 수 있기 때문에 음폐수의 높은 유기물 농도 및 C/N 비를 활용하여 메탄가스 발생량 증가시킬 수 있었다. Tchobanoglous이 제안한 이론적 메탄가스 발생량 예측수식을 적용하여 메탄 및 바이오가스 발생량을 산정한 결과 하수슬러지 단일 혐기소화의 경우 305.6 mLㆍCH4/gㆍVS, 689.4 mLㆍCH4/gㆍVS의 메탄, 바이오가스가 발생하였고 음폐수 : 하수슬러지를 1:9로 혼합한 시료는 약 322 mLㆍCH4/gㆍVS, 3:7시료에서는 약 354 mLㆍCH4/gㆍVS, 5:5시료에서는 약 386 mLㆍCH4/gㆍVS의 메탄가스가 발생하는 것으로 분석되었다. BMP 실험 결과 1:9, 3:7, 5:5 비율로 병합 처리한 경우 각각 약 233, 298, 344 mLㆍCH4/gㆍVS의 메탄가스가 발생하였다. 따라서 음폐수의 혼합비율이 높아질수록 메탄가스 발생량은 증가하였고 하수슬러지와 음폐수의 혼합 비율에 따른 병합처리 시 하수슬러지 단독처리에 비해 다량의 메탄가스가 발생되었다. BMP 실험을 통해 생산된 메탄가스의 누적생산 곡선을 Modified Gompertz model과 first order kinetic model에 적용하여 추정한 결과, 메탄생성량은 Modified Gompertz model에서는 238.5, 302.3, 353.6 mL/gㆍVS 발생하였고 first order kinetic model에서는 242.8, 312.5, 365.5 mL/gㆍVS로 음폐수와의 혼합비율이 증가할수록 높게 나타났으며, 최대 메탄생성속도의 경우 3:7비율에서 48.2 mL/gVSㆍday로 최대 메탄생성 속도를 보였다. first order kinetic model의 1차 반응속도상수 k값은 1:9, 3:7, 5:5 비율에 따라 0.32, 0.22, 0.08day-1 나타났다. 1차 반응속도 상수의 경우 음폐수의 혼합비율이 낮을수록 높게 나타났다. Modified Gompertz와 first order kinetic model 모두 실험결과를 잘 모사하였으며, 실험결과와 모의결과의 적합도를 나타내는 상관계수(R2)의 경우 0.92∼0.98으로 높은 상관성을 나타내었다. We mix food waste leachate and sewage sludge by the proportion of 1:9, 3:7 and 5:5. It turns out that they produced 233, 298 and 344 CH4ㆍmL/gㆍVS of methane gas. The result suggests that as the mixing rate of food waste leachate rises, the methane gas productions increases as well. And more methane gas is made when co-digesting sewage sludge and food waste leachate based on the mixing ratio, rather than digesting only sewage sludge alone. Modified Gompertz and Exponential Model describe the BMP test results that show how methane gas are produced from organic waste. According to the test, higher the mixing rate of food waste leachate is, higher the methane gas productions is. The mixing ratio of food waste leachate that produces the largest volume of methane gas is 3:7. Modified Gompertz model and Exponential model describe the test results very well. The correlation values(R2) that show how the results of model prediction and experiment are close is 0.92 to 0.98.

불안정 지지면을 이용한 운동 프로그램이 뇌졸중 환자의 균형에 미치는 효과

이지연,김용수,김진섭,박진현,최윤희,전덕훈,김경 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

본 연구의 목적은 뇌졸중 환자의 재활을 위하여 불안정 지지면을 이용한 운동 프로그램이 균형 능력과 운동 기능 능력에 미치는 효과를 알아보고자 실시하였다. 불안정 지지면의 이용한 치료는 임상적으로 뇌졸중으로 인한 편마비 환자뿐만 아니라 스포츠 손상 및 노인들의 질환 등 다양한 병인을 가진 환자들의 치료에 이용되고 있다. 대부분의 뇌졸중 환자들은 재활 훈련을 할 수 있는 병원이나 기관에서 치료를 받을 때에 지지면의 차이를 제공함으로써 보다 더 안정한 지지면에서 적응할 수 있는 방법이 요구되고 있다. 하지만, 아직도 불안정 지지면을 이용한 구체화된 치료적 운동 방법과 객관적 평가에 대한 연구가 미비한 실정이다. 이러한 문제점을 해결하기 위하여 최근 뇌졸중 환자의 불안정 지지면과 이론적 근거를 바탕으로 한 운동 프로그램에 대한 연구가 더욱 필요하다. 본 연구에서는 실험군과 대조군이 같은 운동 프로그램을 적용하였을 때, 두 그룹 간에서 나타나는 균형 향상과 운동 기능 향상에 어떠한 효과를 주는지 살펴보고자 하였다. The purpose of this article was to study the effect of the exercise programme that uses the unstable supporting surface to rehabilitate cerebrovascular patients on balance ability and exercise function ability. The therapy is being clinically used for the patients with diverse pathological factors such as hemiplegia caused by CVA, sports injury, and diseases in the elderly. when most patients with CVA are treated in the hospital or institute that can provide rehabilitation training, they need to be given the way to adjust more stable surpporting surface by being provide with various stages of surface. However, the actual therapeutic exercises is still needed and the study for objective assessments wants conducting. To solve these problems, the study of unstable surface exercise programmes based on the evidences recently need to be made for the patients with CVA. in this study, we intend to look into what effects on the balance improvement and the exercise function are different from the experimental group to the control group with the same programme applied to the two groups.

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

PD-1H Is a Novel Negative Checkpoint Regulator for Hepato-cellular Carcinoma Immunotherapy

( Su Jong Yu ),( Kyoung Wan Yoon ),( Young Youn Cho ),( Eun Ju Cho ),( Jeong-hoon Lee ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The field of hepatocellular carcinoma (HCC) immunotherapy has made progress whereas negative checkpoint regulators restrict T-cell responses. Programmed death-1 homolog (PD-1H) has been shown to suppress T-cell responses but little is known in the role of PD-1H on cytokine-induced killer (CIK) cells that are ex vivo expanded T-cells expressing both NK- and T-cell markers. Thus, this study aimed to determine the role of PD-1H in CIK cell immunotherapy targeting HCC. Methods: Murine (MH134) and human (Huh-7) HCC cell lines were cultured under normoxic or hypoxic culture conditions. Murine CIK cells were generated from C3H mice splenocytes and human CIK cells were generated from blood of patients with HCC. RT-PCR and immunoblotting were used to determine the expression of PD-1H. Cytotoxicity assay was performed in the presence or absence of PD-1H siRNA transfection. The potential synergistic effect of CIK cells combined with anti-mouse PD-1H blocking antibody was evaluated in the murine HCC model. Results: Both in mouse and human, PD-1H was expressed on HCC and CIK cells. Moreover, PD-1H was significantly overexpressed in hypoxic than normoxic conditions. Cytotoxicity of CIK cells over HCC cells was significantly suppressed in a hypoxic condition, but blocking of PD-1H by siRNA transfection restored cytotoxicity of CIK cells both in normoxic and hypoxic conditions. Finally, PD-1H blockade augmented antitumor CIK-cell responses in the murine HCC model (P=0.028). Conclusions: These findings show that PD-1H, a novel negative im-munoregulatory molecule, has functional activities that are nonredundant with other Ig superfamily members and may play a role in HCC immunotherapy.

Poster Session 2 : Virology Molecular Medicine and Gene Therapy Procaryotic Genetics Biotechnology ; Molecular characterization of the genes encoding α, β-glucosidase inhibitor production from Bacillus Lentimorbus

( Yoon Kyoung Lee ),( Jang Mi Lee ),( Su Jeong Kim ),( Tae Hoon Kim ),( Kyoung Ja Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2004 생화학분자생물학회 춘계학술발표논문집 Vol.2004 No.-

S-217 : Coronary artery spasm in a patient with iatrogenic hyperthyroidism

( Yoon Kyoung Kim ),( In Kyoung Shim ),( Jung Ho Heo ),( Kyoung Im Cho ),( Hyun Su Kim ),( Jae Woo Lee ),( Tae Joon Cha ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Case We report the first case of coronary artery spasm with iatrogenic hyperthyroidism in Korea. Hyperthyroidism is a rare cause of coronary artery spasm and most of them is associated grave`s disease. Coronary artery spasm with iatrogenic hyperthyroidism is very rare. A 73-year-old woman was admitted to our cardiology department with resting chest pain. She was treated with L-thyroxin (dose 0.15 mg/day) for hypothyroidism because she underwent total thyroidectomy due to thyroid papillary cancer 10 months ago. She had been never smoke and alcohol. Her ECG was no significant change comparing with the ECG 10 months ago. Coronary angiography showed diffuse spasm in the right artery after intravenous ergonovine injection,which resolved with intracoronary nitrate application. Laboratory data showed the following: thyroid-stimulating hormone (TSH) was undetectable < 0.004 μIU/mL (normal range: 0.35-5.5 μIU/mL), Free T4 was 2.28 ng/dL (normal range: 0.78-1.54 ng/dL), T3 was 148.21 ng/dL (65-150 ng/dL). She was subsequently diagnosed with hyperthyroidism secondary to L-thyroxine which is thought to be the cause of the coronary spasm. Symptoms resolved upon treatment with diltiazem and isosorbide mononitrate. She was stable at the 2-year follow-up.

Characterization of the aminopyridine derivative KRC-180 as a JAK2 inhibitor

Yoon, Kyoung Bin,Cho, Sung Yun,An, Su Jin,Park, Kyeong Ryang,Lee, Hyo Jeong,Yoon, Hae Sung,Lee, Sun-Mi,Kim, Yong-Chul,Han, Sun-Young D.A. Spandidos 2017 Oncology letters Vol.14 No.2

<P>Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that regulates the signal transducer and activator of transcription (STAT) signaling pathway. Deregulation of JAK2 signaling has previously been observed in hematologic malignancies, including erythroleukemia. In the present study, an aminopyridine derivative compound, KRC-180, exhibited direct inhibition of the JAK2 protein at the catalytic site, as demonstrated using <I>in vitro</I> kinase activity assays and docking analyses. In addition, KRC-180 reduced the phosphorylation of STAT3 and STAT5, downstream signaling molecules of JAK2. The growth of HEL92.1.7 erythroleukemia cells harboring a constitutively activated form of JAK2 was suppressed by KRC-180 treatment; KRC-180 induced apoptotic cell death and cell cycle arrest. The results of the present study indicate that KRC-180 is a JAK2 inhibitor with anti-leukemic properties.</P>

Tumor Mesenchymal Stem-Like Cell as a Prognostic Marker in Primary Glioblastoma

Yoon, Seon-Jin,Shim, Jin-Kyoung,Chang, Jong Hee,Moon, Ju Hyung,Roh, Tae-Hoon,Sung, Kyoung Su,Lee, Ji-Hyun,Kim, Eui-Hyun,Kim, Sun Ho,Hong, Yong-Kil,Lee, Su-Jae,Huh, Yong-Min,Kang, Seok-Gu Hindawi Publishing Corporation 2016 Stem cells international Vol.2016 No.-

<P>The isolation from brain tumors of tumor mesenchymal stem-like cells (tMSLCs) suggests that these cells play a role in creating a microenvironment for tumor initiation and progression. The clinical characteristics of patients with primary glioblastoma (pGBM) positive for tMSLCs have not been determined. This study analyzed samples from 82 patients with pGBM who had undergone tumor removal, pathological diagnosis, and isolation of tMSLC from April 2009 to October 2014. Survival, extent of resection, molecular markers, and tMSLC culture results were statistically evaluated. Median overall survival was 18.6 months, 15.0 months in tMSLC-positive patients and 29.5 months in tMSLC-negative patients (<I>P</I> = 0.014). Multivariate cox regression model showed isolation of tMSLC (OR = 2.5, 95% CI = 1.1~5.6, <I>P</I> = 0.021) showed poor outcome while larger extent of resection (OR = 0.5, 95% CI = 0.2~0.8, <I>P</I> = 0.011) has association with better outcome. The presence of tMSLCs isolated from the specimen of pGBM is associated with the survival of patient.</P>

EFFECT OF ION BALANCE IN SAHARA DESERT ON GROWTH PERFORMANCE OF PACIFIC WHITE SHRIMP Litopenaeus vannamei

Kim Su Kyoung,Hong-Seok Yoon,Won- Jo Choi,Su-Kyoung Kim,In-Kwon Jang 한국수산해양기술학회(구 한국어업기술학회) 2017 한국수산해양기술학회 학술발표대회 Vol.2017 No.11

직접토크 제어를 이용한 유도전동기의 센서리스 속도제어에 관한 연구

윤경국(Kyoung-Kuk Yoon),오세진(Sae-Gin Oh),김종수(Jong-Su Kim),김윤식(Yoon-Sik Kim),이성근(Sung-Gun Lee),김성환(Sung-Hwan Kim) 한국마린엔지니어링학회 2009 한국마린엔지니어링학회지 Vol.33 No.8