부인암 환자에서 발견되는 대장 선종의 특징

전정현,정성애,김성은,이종수,남승현,신정은,문혜성,김승철,유권 梨花女子大學校 醫科大學 醫科學硏究所 2003 EMJ (Ewha medical journal) Vol.26 No.1

연구목적 : 2형 Lynch 증후군에 대한 관심이 높아지면서 부인과 종양 환자에게 대장암과 대상 선종의 검사 필요성이 언급되어지고 있다. 본 연구에서는 본원의 부인과 종양 환자에서 병기 결정을 위해 시행되어지는 S상 결장경 검사에서 발견되는 대상 선종의 특징을 알아보고자 하였다. 방법 : 1993년 9월부터 2001년 3월까지 본원에서 부인과 종양을 진단받고 대장 증상 없이 병기 결정을 위해 S상 결장경검사를 시행한 187명을 대상으로 하였고(자궁경부암 139명, 난소암 35명, 자궁 내막암 13명) 정상대조군으로는 대장 증상 없이 건강 검진 목적으로 대장경 검사를 시행한 58명을 대상으로 하였다. 결과 : 부인과 종양 환자 187명 중 21명에서 대장 선종이 발견되었고(11.2%) 다발성 선종이 있어 모두 26예의 선종이 관찰되었다. 정상 대조군 58명에서는 3명에서 선종이 발견되었고(5.2%) 다발성 선종은 없었다. 부인과 종양 중 자궁 내막암 환자군에서 선종의 빈도는 38.5%로 정상 대조군에 비해 통계학적으로 유의하게 많았다. 진행성 선종의 빈도도 자궁 경부암 환자 12.5%, 난소암 환자 25.5%, 정상 대조군 33.3%에 비해 자궁 내막암 환자 83.5%로 유의하게 많았다. 부인과 종양의 선종 위치는 직장이 23.1%, S상결장이 76.9%였다. 결론 : 부인과 종양 환자의 대장 선종 빈도는 정상 대조군에 비해 많은 경향을 보였으나 통계학적 의의는 없었다. 그러나 자궁 내막암 환자군에서는 다른 부인과 종양 환자나 정상 대조군에 비해 유의하게 많은 선종과 진행성 선종의 빈도를 부였다. 따라서 부인과 종양 환자에게 대장경 검사를 통한 대장 선종 유무 검사가 필요하리라 생각된다. Objectives : In Lynch syndrome Ⅱ, colon cancer was associated with endometrial and ovarian cancer. The aim of this study was an evaluation for the clinicopathologic characteristics of rectosigmoid adenomas on preoperative sigmoidoscopy in gynecologic cancer patients. Methods : A total 187 gynecologic cancer patients(139 cervical, 35 ovarian, 13 endometrial cancer) and 58 normal controls were reviewed sigmoidscopic finding and pathologic reports retrospectively from September 1993 to March 2001. Results : The mean age of gynecologic cancer patients was 54(38-82) year-old and normal controls was 50(20-68) year-old. Total 26 adenomas were in 21 patients(11.2%) and 3 adenomas were in 3 normal controls(5.2%). The incidence of adenomas was 9.4% in cervical cancer, 8.6% in ovarian cancer and 38.5% in endometrial cancer. Multiple adenomas were in 5 gynecologic cancer patients and 0 normal controls. The incidence of advanced adenoma was 12.5% in cervical cancer, 25.5% in ovarian cancer, 83.5% in endometrial cancer and 33.3% in normal controls. The location of adenoma was 23.1% in rectum and 76.9% in sigmoid colon. Conclusion : The incidence of adenomas and multiple adenomas were higher in gynecologic cancer patients than normal controls but not significantly. The incidence of advanced adenoma and adenomas were significantly higher in endometrial cancer than normal controls. Colonoscopic evaluation of whole colon will be recommanded in gynecologic cancer than sigmoidoscopy.

열대 지역에서 통일형 초다수성 벼 품종의 생육 및 수량성 비교

이지윤,준현,성환,손영보,송유천,한상익,박동수,권영업,이철원,이종희 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

의사국가시험성적과의 상관관계로 살펴본 임상종합평가시험의 가치 : 2002년 연세대 원주의대 졸업생을 대상으로

김명수,김춘배,차병호,박기창,권상옥,신계철,이혜용,강성준,차봉석 한국보건의료인국가시험원 2004 보건의료교육평가 Vol.1 No.1

Korean Medical Licensing Examination(KMLE) 2002 focused on evaluation of the integrative medical knowledge such as primary clinical care or problem-solving competence. We analyzed the correlation among the year-wise student academic scores(grade score), trial examination scores and KMLE score by correlation analysis and multiple regression method. Four times of trial examination were taken in 2001, which were composed according to the principles of KMLE. Trial examination scores were significantly correlated with student grade scores (p(0.05). KMLE score also correlated with student grade score a nd trial examination score. The grade score at senior had higher correlation coefficient than the grade score at junior in correlation analysis. In multiple regressions, grade score at senior and mean score of trial examinatio n score were significant variants affecting KMLE score. Based on this result, regression formula such as [KMLE score] = 110.596+21.449^*[6th grade score of student] + 0.577^*[mean of trial examination score] was established (R2=0.764, p<0.001). Our results show that the trial examination is useful evaluation tool for final assessment of medical achievements. Also a trial examination is used as a reference data for student guidance and control in preparing for KMLE.

Gastroprotective effects of irsogladine maleate on ethanol/hydrochloric acid induced gastric ulcers in mice

( Seong Chun Kwon ),( Ji Hoon Kim ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.1

Background/Aims: This study was conducted to investigate the inhibitory effect of irsogladine maleate (IM) on gastric ulcers induced by ethanol and hydrochloric acid (HCl). Methods: Mice were pretreated with IM for 1 hours before ulcer induction. Gastric ulcers were induced by oral administration of an ethanol/HCl mixture. To clarify the action mechanism of IM, the roles of 3ʹ5ʹ-cyclic adenosine monophosphate (cAMP), nitric oxide (NO), adenosine triphosphate-sensitive potassium (K_ATP) channels, prostaglandins and transient receptor potential cation channel subfamily V member 1 (TRPV1) were investigated, and lipid peroxidation in the stomach of IM-treated and -untreated animals was also measured. Results: IM significantly reduced the extent of ethanol/HCl mixture-induced gastric ulceration. It exhibited dose-related gastroprotection against the ethanol/HCl-induced lesions, while pretreatment with glibenclamide but not N(ω)-nitro-L-arginine methyl ester, reversed this action. While pretreatment with the TRPV1 antagonist capsazepine failed to effectively block the gastroprotective effect of IM, the non-selective cyclooxygenase inhibitor indomethacin almost abolished it. IM also decreased the level of thiobarbituric acid reactive substances. Conclusions: We concluded that IM exhibited significant gastroprotective effects in an ethanol/HCl-induced ulcer model, which appear to be mediated, at least in part, by NO, cAMP, endogenous prostaglandins, K_ATP channel opening, activation of TRPV1 channels, and antioxidant properties.

The Effect of NO Donor on Contraction, Cytosolic Ca<SUP>2⁢</SUP> Level and Ionic Currents in Guinea-pig Ileal Smooth Muscle

Seong-Chun Kwon,Ki-Young Park,Duck-Sun Ahn,Young-Ho Lee,Bok-Soon Kang 대한생리학회-대한약리학회 2000 The Korean Journal of Physiology & Pharmacology Vol.4 No.1

<P> This study was designed to clarify the mechanism of the inhibitory action of a nitric oxide (NO) donor, 3-morpholino-sydnonimine (SIN-1), on contraction, cytosolic Ca<SUP>2⁢</SUP> level ([Ca<SUP>2⁢</SUP>]<SUB>i</SUB>), and ionic currents in guinea-pig ileum. SIN-1 (0.01∼100μM) inhibited 25 mM KCl- or histamine (10μM)-induced contraction in a concentration-dependent manner. SIN-1 reduced both the 25 mM KCl- and the histamine-stimulated increases in muscle tension in parallel with decreased [Ca<SUP>2⁢</SUP>]<SUB>i</SUB>. Using the patch clamp technique with a holding potential of ⁣60 mV, SIN-1 (10μM) decreased peak Ba currents (I<SUB>Ba</SUB>) by 30.9⁑5.4% (n=6) when voltage was stepped from ⁣60 mV to ⁢10 mV and this effect was blocked by ODQ (1μM), a soluble guanylyl cyclase inhibitor. Cu/Zn SOD (100 U/ml), the free radical scavenger, had little effect on basal I<SUB>Ba</SUB>, and SIN-1 (10μM) inhibited peak I<SUB>Ba</SUB> by 32.4⁑5.8% (n=5) in the presence of Cu/Zn SOD. In a cell clamped at a holding-potential of ⁣40 mV, application of 10μM histamine induced an inward current. The histamine-induced inward current was markedly and reversibly inhibited by 10μM SIN-1, and this effect was abolished by ODQ (1μM). In addition, SIN-1 markedly increased the depolarization-activated outward K<SUP>⁢</SUP> currents in the all potential ranges. We concluded that SIN-1 inhibits smooth muscle contraction mainly by decreasing [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> resulted from the inhibition of L-type Ca<SUP>2⁢</SUP> channels and the inhibition of nonselective cation currents and/or by the activation of K<SUP>⁢</SUP> currents via a cGMP-dependent pathway.

The Effect of Carbon Monoxide on Contraction, Cytosolic Ca<SUP>2⁢</SUP> Level and Ionic Currents in Guinea Pig Ileal Smooth Muscle

Seong Chun Kwon,Seung Soo Chung,Yun Suk Kim,Taick Sang Nam 대한생리학회-대한약리학회 2000 The Korean Journal of Physiology & Pharmacology Vol.4 No.6

<P> The aim of this study was to clarify the mechanism of the inhibitory action of carbon monoxide (CO) on contraction, by measuring cytosolic Ca<SUP>2⁢</SUP> level ([Ca<SUP>2⁢</SUP>]<SUB>i</SUB>) and ionic currents in guinea-pig ileum. CO (10%) inhibited 40 mM KCl-induced contraction and this effect was blocked by ODQ (1μM), a soluble guanylyl cyclase (sGC) inhibitor. CO inhibited the 40 mM KCl-induced contraction without changing [Ca<SUP>2</SUP><SUP>⁢</SUP>]<SUB>i</SUB>. Cumulative addition of KCl induced a graded increase in [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> and muscle tension. In the presence of CO, cumulative addition of KCl induced smaller contraction than in the absence of CO. On the other hand, the increase in [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> induced by cumulative addition of KCl was only slightly decreased in the presence of CO, and the [Ca<SUP>2⁢</SUP>]<SUB>i</SUB>-tension relationship shifted downwards. Using the patch clamp technique with a holding potential of ⁣60 mV, we found that CO had little effect on the peak Ba currents (I<SUB>Ba</SUB>) when voltage was stepped from ⁣60 mV to 0 mV. In addition, CO showed no effect on the depolarization-activated outward K<SUP>⁢</SUP> currents in the all potential ranges. We conclude that CO inhibits smooth muscle contraction mainly by decreasing the Ca<SUP>2⁢</SUP> sensitivity of contractile elements via a cGMP-dependent pathway, not by involving L-type Ca<SUP>2⁢</SUP> and outward-potassium currents in guinea-pig ileum.

Lysophosphatidylcholine Attenuates Endothelium-dependent Relaxation Responses through Inhibition of ACh-induced Endothelial $[Ca^{2+}]_i$ Increase

Kwon, Seong-Chun,Lee, Yong-Ho,Nam, Taick-Sang,Ahn, Duck-Sun The Korean Society of Pharmacology 2006 The Korean Journal of Physiology & Pharmacology Vol.10 No.1

Lysophosphatidylcholine (LPC), which accumulates in atherosclerotic arteries, has been reported to inhibit endothelium-dependent relaxation (EDR) in many different species. However, the underlying mechanism of LPC-induced inhibition of EDR is still uncertain. In the present study, we measured simultaneously both isometric tension and cytosolic free $Ca^{2+}$ ($[Ca^{2+}]_i$) in rabbit carotid strips, and examined the effect of LPC on tension and $[Ca^{2+}]_i$. In carotid strips with intact-endothelium, high $K^+$ (70 mM) increased both tension and $[Ca^{2+}]_i$, and cumulative addition of acetylcholine (ACh) from 0.1 to $10{\mu}M$ induced dose dependent increase of $[Ca^{2+}]_i$ with concomitant relaxation. In the presence of L-NAME (0.1 mM), ACh increased $[Ca^{2+}]_i$ without affecting the amplitude of high $K^+-induced$ tension. These ACh-induced change of $[Ca^{2+}]_i$ and tension was abolished by removal of endothelium or 10 nM 4-DAMP (muscarinic receptor antagonist) pretreatment. Pretreatment of LPC ($10{\mu}M$) inhibited ACh ($10{\mu}M$)-induced change of tension and $[Ca^{2+}]_i$ in endothelium-intact carotid artery. On the other hand, LPC had no effect on ACh-induced change of tension and $[Ca^{2+}]_i$ in endothelium denuded artery. In $Ca^{2+}$-free external solution, ACh transiently increased $[Ca^{2+}]_i$, and pretreatment of LPC significantly inhibited ACh-induced transient $[Ca^{2+}]_i$ change. Based on the above results, it may be concluded that LPC inhibits the ACh-induced $[Ca^{2+}]_i$ change through inhibition of $Ca^{2+}$ mobilization in vascular endothelial cells, resulting in decreased production of NO and concomitant inhibition of endotheliumdependent vascular relaxation.

Proteomic Analysis of Colonic Mucosal Tissue from Tuberculous and Ulcerative Colitis Patients

Kwon, Seong-Chun,Won, Kyung-Jong,Jung, Seoung-Hyo,Lee, Kang-Pa,Lee, Dong-Youb,Park, Eun-Seok,Kim, Bok-Yung,Cheon, Gab-Jin,Han, Koon-Hee The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.3

Changes in the expression profiles of specific proteins leads to serious human diseases, including colitis. The proteomic changes related to colitis and the differential expression between tuberculous (TC) and ulcerative colitis (UC) in colon tissue from colitis patients has not been defined. We therefore performed a proteomic analysis of human TC and UC mucosal tissue. Total protein was obtained from the colon mucosal tissue of normal, TC, and UC patients, and resolved by 2-dimensional electrophoresis (2-DE). The results were analyzed with PDQuest using silver staining. We used matrix-assisted laser desorption ionization time-of-flight/time-of-flight spectrometry (MALDI TOF/TOF) to identify proteins differentially expressed in TC and UC. Of the over 1,000 proteins isolated, three in TC tissue and two in UC tissue displayed altered expression when compared to normal tissue. Moreover, two proteins were differentially expressed in a comparative analysis between TC and UC. These were identified as mutant ${\beta}$-actin, ${\alpha}$-enolase and Charcot-Leyden crystal protein. In particular, the expression of ${\alpha}$-enolase was significantly greater in TC compared with normal tissue, but decreased in comparison to UC, implying that ${\alpha}$-enolase may represent a biomarker for differential diagnosis of TC and UC. This study therefore provides a valuable resource for the molecular and diagnostic analysis of human colitis.

Modulation of Outward Potassium Currents by Nitric Oxide in Longitudinal Smooth Muscle Cells of Guinea-pig Ileum

Seong-Chun Kwon,Se Joong Rim,Bok-Soon Kang 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.2

<P> To investigate the possible involvement of outward potassium (K<SUP>+</SUP>) currents in nitric oxide-induced relaxation in intestinal smooth muscle, we used whole-cell patch clamp technique in freshly dispersed guinea-pig ileum longitudinal smooth muscle cells. When cells were held at ⁣60 mV and depolarized from ⁣40 mV to ⁢50 mV in 10 mV increments, sustained outward K<SUP>+</SUP> currents were evoked. The outward K<SUP>+</SUP> currents were markedly increased by the addition of 10 ㄍM sodium nitroprusside (SNP). 10 ㄍM S-nitroso-N-acetylpenicillamine (SNAP) and 1 mM 8-Bromo-cyclic GMP (8-Br-cGMP) also showed a similar effect to that of SNP. 1 mM tetraethylammonium (TEA) significantly reduced depolarization-activated outward K<SUP>+</SUP> currents. SNP-enhanced outward K<SUP>+</SUP> currents were blocked by the application of TEA. High EGTA containing pipette solution (10 mM) reduced the control currents and also inhibited the SNP-enhanced outward K<SUP>+</SUP> currents. 5 mM 4-aminopyridine (4-AP) significantly reduced the control currents but showed no effect on SNP-enhanced outward K<SUP>+</SUP> currents. 0.3 ㄍM apamin and 10 ㄍM glibenclamide showed no effect on SNP-enhanced outward K<SUP>+</SUP> currents. 10 ㄍM 1H-[1,2,4]oxadiazolo [4,3-a]quinoxaline-1-one (ODQ), a specific inhibitor of soluble guanylate cyclase, significantly blocked SNP-enhanced K<SUP>+</SUP> currents. We conclude that NO donors activate the Ca<SUP>2+</SUP>-activated K<SUP>+</SUP> channels in guinea-pig ileal smooth muscle via activation of guanylate cyclase.

Lysophosphatidylcholine Attenuates Endothelium-dependent Relaxation Responses through Inhibition of ACh-induced Endothelial [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> Increase

Seong-Chun Kwon,Yong-Ho Lee,Taicksang Nam,Duck-Sun Ahn 대한생리학회-대한약리학회 2006 The Korean Journal of Physiology & Pharmacology Vol.10 No.1

Lysophosphatidylcholine (LPC), which accumulates in atherosclerotic arteries, has been reported to inhibit endothelium-dependent relaxation (EDR) in many different species. However, the underlying mechanism of LPC-induced inhibition of EDR is still uncertain. In the present study, we measured simultaneously both isometric tension and cytosolic free Ca<SUP>2⁢</SUP> ([Ca<SUP>2⁢</SUP>]<SUB>i</SUB>) in rabbit carotid strips, and examined the effect of LPC on tension and [Ca<SUP>2⁢</SUP>]<SUB>i</SUB>. In carotid strips with intact-endothelium, high K<SUP>⁢</SUP> (70 mM) increased both tension and [Ca<SUP>2⁢</SUP>]<SUB>i</SUB>, and cumulative addition of acetylcholine (ACh) from 0.1 to 10μM induced dose dependent increase of [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> with concomitant relaxation. In the presence of L-NAME (0.1 mM), ACh increased [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> without affecting the amplitude of high K<SUP>⁢</SUP>-induced tension. These ACh-induced change of [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> and tension was abolished by removal of endothelium or 10 nM 4-DAMP (muscarinic receptor antagonist) pretreatment. Pretreatment of LPC (10μM) inhibited ACh (10μM)-induced change of tension and [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> in endothelium-intact carotid artery. On the other hand, LPC had no effect on ACh-induced change of tension and [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> in endothelium denuded artery. In Ca<SUP>2⁢</SUP>-free external solution, ACh transiently increased [Ca<SUP>2⁢</SUP>]<SUB>i</SUB>, and pretreatment of LPC significantly inhibited ACh-induced transient [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> change. Based on the above results, it may be concluded that LPC inhibits the ACh-induced [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> change through inhibition of Ca<SUP>2⁢</SUP> mobilization in vascular endothelial cells, resulting in decreased production of NO and concomitant inhibition of endothelium- dependent vascular relaxation.