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문혜성,김상술,민병조,서재걸,성순희 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.11
Objective : Angiogenesis is a critical factor in the progression of solid tumors. The mechanisms responsible for angiogenesis in cervical neoplasia, however, are not well defined. Our study was aimed to determine the expression of VEGF(Vascular Endothelial Growth Factor), its receptor(KDR), and TGF-β1(Transforming Growth Factor-β1) in cervical neoplasia, to determine the role of these angiogenic factors in preinvasive(dysplastic) process and the progression of cervical cancer and to investigate the progression of angiogenesis in the transition from normal cervix to invasive squamous cell carcinoma of the uterine cervix. Methods : The cervical lesions of 76 patients were punch biopsied and paraffin embedded. Among these, 5 were normal cervix, 36 were cervical intraepithelial lesion I-III, and the other 35 were invasive squamous cell carcinomas. The tissues were immunostained with antiVEGF, antiKDR, and antiTGF-β1 polyclonal antibody. Results : The expression of VEGF, KDR, and TGF-β1 in CIN III was stronger than those of CIN I(p$lt;0.01). Their expression were not significantly different among the each staged cervical cancers(p$gt;0.01). Conclusions : These observations suggest that VEGF, KDR, and TGF-β1 are important angiogenic factors in cervical neoplasia, especially in an early event to neoplastic transformation of cervical tissues, but these angiogenic factors are not associated with the progression of cervical cancer.
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문혜성,정윤재 이화여자대학교 의과대학 1985 梨花醫學誌 Vol.- No.17
The present study was done to describe normal ultrastructure and characteristics in the marmot substantia nigra. The marmot was perfused with 2.5% glutaraldehyde fixative in 0.1M phosphate buffer. Tissue from substantia nigra was postfixed with 1% osmium tetroxide in 0.1M phosphate buffer and dehydrated in graded ethyl alcohol and acetone, and embedded in Epon 812. The polymerization was carried out in 60℃ oven for 3days. The ultrathin sections were contrasted with uranyl acetate and lead citrate, and examined with Hitachi-H600 TEM. 1) The neurons of the substantia nigra had many organelles like other neurons do, but especially they had very many lysosomes in the cytoplasm and had nucleus with deep indentation. 2) The dendrites of neurons contained many filaments, endoplasmic reticulum, and ribosomes and they had few dendritic spine. 3) Most of the synapses in the substantia nigra were axodendritic and axo-somatic synapses but some of them were specially encircled synapses.
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한국 가톨릭신학대학교 미디어교육 교육과정 도입을 위한 요구분석과 종교미디어교육 연구
문혜성,박진성 한국가톨릭신학학회 2008 가톨릭신학 Vol.0 No.12
Priests are being influenced strongly by mess media to carry out pastoral care today. So they should be educated to use media effectively and to understand what is the cultural meaning of media properly. In addition to, they have to have an professional and systematic education of media in a seminary so that they can protect themselves and believers from unethical aspects of media and can se media effectively for evangelization. This is a investigation to introduce media education in a curriculum of seminaries in the Catholic Universities in Korea. It is significant in that this is a specific and systematic investigation to find a necessity and the way of media education which priests and seminary demand. This was a basic research to develop courses of media education for seminary students and included questions as follows. These results suggest, most of the priests and seminary students thought that the media education was just an assistant education or a means for evangelization. It is necessary to change people's under- standing of the media education who charge in education to set up media education in a curriculum of the seminaries. It is also needed to train expert who can teach the media professionally and systematically and to support economically. It should not be stressful to seminary students who have a lot of credits as well. The education methods and contents in the Catholic Universities should be the religious media education. It is necessary to development the media competence of priests and the students. The religious media education were integrated with the teaching plan model in a curri- culum of the seminaries. In order for this education to be applied effec- tively, experimental studies have to be carried out verifying the learning effects regarding this education. In particular, education for teachers who will carry out this education is needed. This is because, in media education, the efficiency of education is significantly affected by the capacity of the one who is teaching, the media competence of priests. 가톨릭교회에서는 여러 문헌과 연구를 통해 만민을 대상으로 하는 미디어를 사회·교육적으로 올바르고 효율적으로 활용하여 복음선포의 사명을 충실히 이행할 것을 강조하고 있다. 이 연구에서는 한국 가톨릭대학교에 미디어교육이 도입될 수 있는 방안을 모색하고자 한다. 그 이론적 배경으로써 가톨릭교회의 가르침과 미디어와의 관계, 그리고 한국 가톨릭대학의 교육목표와 교육과정을 살펴보고, 종교미디어교육의 개념을 정리하였다. 또한 신학생과 사제들을 대상으로 한국 가톨릭대학교에서의 미디어교육의 필요성과 이의 도입을 위한 요구분석을 수행하였다. 요구분석 결과, 상당수의 신학생과 사제들은 대학교 내 미디어교육의 필요성에 대해 매우 높은 요구를 나타내었다. 대부분의 신학생들과 사제들은 이 교육의 필요성 뿐 아니라, 원하는 교육의 방향도 매우 구체적으로 인지하고 있었다. 미디어교육 교육내용에 대하여 이들은 실질적으로 미디어를 활용·제작할 수 있는 능력을 고양시킬 수 있는 내용을 원하였다. 또한 왜곡된 종교문화 및 윤리적 사항을 정확히 파악할 수 있는 내용으로 구성되기를 원하였다. 이 교육에 적합한 교수자로서는 미디어교육학 전문가를 꼽았다. 교과과정에 미디어교육 교육과정이 도입되기 위한 선행과제로써 신학생, 교수신부, 신학대학교 운영에 책임을 가지고 있는 성직위원회 주교들의 인식변화가 우선되어야 한다는 사실에 공감하고 있었다. 또한 많은 사제들은 미디어교육 전문가양성 프로그램에 참여할 의사를 밝혔다. 궁극적으로 가톨릭신학대학교의 미디어교육 형태는 다른 일반 교육기관과 차별화 하여, 그리스도교의 신념을 바탕으로 한 실용적 종교미디어교육의 방향으로 전개되어야 할 것이다. 종교미디어교육의 교육목표는 종교의 근본 가치 및 미디어문화와 일상생활세계가 보여주는 세계를 차별화하여 인지하는 미디어능력을 촉진하는 것이다. 미디어나 종교커뮤니케이션에 대한 개인의 해석능력과 인지능력을 촉진하고, 미디어의 기능과 전달내용 및 종교와 종교의 특성이 전달하는 내용에 대한 이해와 비평적 판단능력을 촉진하고자 한다. 나아가 창의적인 프로그램을 자율적으로 제작하는 능력을 키우고, 미디어 활용에 사회윤리적으로 책임있는 행동과 토론의 능력을 키우면서, 다양한 의견을 형성할 수 있는 능력을 촉진하고자 한다. 이러한 교육목표를 구체적으로 교육할 수 있는 종교미디어교수법, 상징교수법과 전문가교육(사제양성교육)의 활성화도 중요한 교육목표가 된다.
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Nd:YAG 레이저조사 후 치근의 처치방법들이 치근면 치은섬유아세포부착에 미치는 영향에 관한 연구
문혜성,임기정,김병옥,한경윤,Moon, Hye-Seong,Lim, Kee-Jung,Kim, Byung-Ock,Han, Kyung-Yoon 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.3
The purpose of this study was to evaluate the biocompatibility of the Nd:YAG lased root surface followed by root planing and/or tetracyline-HCI(T.C.-HCI) conditioning. $30,4mm{\times}4mm$ root segments were obtained from unerupted third molars and 21, periodontally involved root segments. The treatment groups were as follows : (1) healthy root cementum surface groups : 1) control(non-treated group), 2) lased only, 3) lased/root planed, and 4) lased/T.C.-HCI. (2) diseased root cementum surface groups : 1) control(root planed only), 2) lased/root planed, and 3) lased/root planed/T.C.-HCI. The specimens were treated with a Nd:YAG laser using a $320{\mu}m$ noncontact optic fiber handpiece with an energy setting of 1.5W($114.6J/cm^2$), 2.0W($152.9J/cm^2$), 5.0W($382J/cm^2$) for one minute. The fiber was held perpendicular to the petri dish(NUNC) 2cm apart in an attempt to expose the entire root segments equally. Human gingival fibroblasts were cultured from explants of normal interdental gingival tissue obtained during third morlar extraction. The attachment assay was performed with third-generation fibroblasts. The numbers of gingival fibroblasts attached to the root surface were counted on each specimen under the light microscope, and were statistically analyzed by the oneway ANOVA followed by Tukey's test in SPSS/PC+programs. The results were as follows : 1) In healthy root cementum surfaces, lased/root planed groups exhibited a significantly increased fibroblast attachment compared to controls, lased only, and lased/T.C.-HCI groups(p<0.05), 2) In diseased root cementum surfaces, laser treatment followed by root planing and/or T.C.HCl groups exhibited a increased tendency of fibroblast attachment compared to root planed only group. The results suggest that laser treatment followed by root planing and/or T.C.-HCl would appear necessary so as to render the root surface biocompatible.
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