151종 생약제 추출물이 B형 간염 바이러스 표면 항원(HBsAg)과 항체(HBsAb)의 결합반응 그리고 HBV DNA polymerase 활성에 미치는 효과 : 한국산 각종 생약제를 이용한 B형 간염치료제의 개발

정태호,김정철,김문규,이인선,채성철,김승래,정준모,이인수,김승호,함경수 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

세계인구 43억명 중 B형 간염 바이러스 보균자는 2억 1천 5백만명이며 이중 78%인 1억 6천만명 이상이 아시아 대륙에 집중되어 있다. 한국도 B형 바이러스 간염의 침윤지대이다. 본 연구는 B형 바이러스 보균자 혹은 만성간염을 치료할 수 있는 생약제를 탐색할 목적의 일환으로 한국에서 시판하는 생약제 151종을 구입하여 증류수로 추출하고 여과한 후 냉동건조하여 HBs 항원에 대한 결합능, HBV DNA polymerase억제능, tumor necrosis factor(TNF) 유리에 미치는 영향, 그리고 galactosamine 유발 간염에 대한간장보호 작용을 조사하였다. 151종의 생약제중 0.16㎍/㎖ 농도에서 간염 vaccine 내에 함유된 HBs 항원과 결합하는 생약제는 지유, 가자, 복분자, 대황, 정향, 빈량, 산수유, 소목, 마황, 쇄양, 후박, 목과, 양강, 오약, 산사, 목단피 등 16종이었다. 이들 생약제중 대황(Rheum palmatum L. 大黃), 가자(Terminalia chebula R. 訶子), 지유(Sanguisorba officinalis L. 地楡), 복분자(Rubus coreanus M. 覆盆子)는 HBV DNA polymerase 활성도를 비교적 강하게 억제하였으며 빈량(Areca catechu L. 빈랑), 정향(Eugenia caryophyllata T. 丁香), 목과(Chaenomeles japonica L. 木瓜), 산수유(Cornus officinalis S. 山茱萸)는 약하게나마 HBV DNA polymerase 활성을 억제하였다. 또한 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 대상으로 TNF 유리에 미치는 영향을 조사한 결과 소목을 제외한 8종의 생약제 모두가 대식세포를 자극하여 TNF를 유리시켰다. 그리고 장차 임상에 사용함을 전제로 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 galactosamine 유발 간염(백서)에 투여하여 간기능 검사에 미치는 효과를 조사하였으나 간기능 검사상으로는 특별한 보호작용을 나타내지 못하였으며 생약제 단독 투여로서는 간기능에 대하여 나쁜 영향을 주지 않았다. Hepatitis B virus infection is major cause of acute and chrome hepatitis, cirrhosis of liver, and hepatocellular carcinoma. There are over 200 million carriers of hepatitis B virus in the world, nearly 80 % of whom live in the South East Asia, Korea is also highly endemic area of hepatitis B virus infection. This study was performed to evaluate the effect of extracts of traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBs Ag, inhibition of HBV DNA polymerase, release of tumor necrosis factor (TNF), and protection against galactosamine induced liver damage. Among 151 herbs, 16 herbs (Sanguisorba officinalis L., Terminalia chebula R., rubus coreanus M., Rheum palmatum L., Eugenia caryophyllata T., Areca catechu L., Cornus officinalis S., Caesalpinia sappan L, Ephedra sinica S., Cynomorium songaricum R., Machilus thunbergii S., Chaenomeles japonica L., Alpinia officinarum H., Lindera strychnifolia V., Crataegus pinnatifida B., Paeonia suffruticasa A. ) had binding capability to HBs Ag which contained in the commercial hepatitis vaccine, at 16 ug/㎖ of concentratioa Among the above 16 herbs, Rheum palmatum L., Terminalia chebula R., Sanguisorba officinalis L., Rubus coreanus M. significantly inhibited the HBV DNA polymerase activity, and Areca catechu L., Eugenia caryophyllata T., Ephedra sinica S., Caesalpinia sappan L., Chaenomeles Japonica L., Cornus officinalis S. also inibited the HBV DNA polymerase activity but less effective. All of the herbs, except Caesalpinia sappan L., which inhibited the HBV DNA polymerase activity, actively stimulated the mononuclear cells to release tumor necrosis factor(TNF) in whole blood culture system. The herbs which inhibited the HBV DNA polymerase activity, did not show any protective effect in galactosamine-induced hepatitis in rats, but there was no direct toxic effect when herbs were administered alone.

GaN분말로부터 합성된β-Ga₂O₃리본 fiber의 특성

김선태 한밭대학교 2004 한밭대학교 논문집 Vol.21 No.-

이 논문에서는 GaN 분말과 산소가 반응하여 합성된 백색의 ß-Ga₂0₃ 리본 fiber에 대하여 몇 가지 성질을 조사하였다. 합성된 리본 fiber에 대한 X-선 회절 패턴으로부터 결정구조는 단사정계의 ß-Ga₂0₃ 임이 확인되었으며, 10 K의 온도에서 측정된 광루미네센스 스펙트럼은 Self-activated optical center에 의해 3.464 eV에서 피크의 반치폭이 48 meV인 강한 강도의 발광과 428, 510, 및 659 nm의 위치에서 불순물과 관련된 발광이 함께 관찰되었다. 이와 같은 광학적 성질과 ß-Ga₂0₃의 전기적 전도성을 이용하여 가시광 영역에서 동작하는 광전소자를 제조할 수 있을 것이다. In this work, we investigated on the white-colored ribbon fiber synthesized from GaN powder. We convinced the formation of monoclinic phase β-Ga₂0₃ from the X-ray diffraction pattern on ribbon fiber. The 10 K PL spectrum consisted with the strong emission band caused by self-activated optical center at 3.464 eV with the full-width at half maximum of 48 meV and the impurity related emission bands at 428, 510, and 659 nm, respectively. From the view point of the optical properties and the electrical conductivity of β-Ga₂0₃, it will be useful for the fabrication of optoelctronic devices operating in visible spectrum region.

한국어판 지역사회 치매선별 검사도구[CSID-K]의 개발

김재민,김성완,신일선,정태길,윤진상 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : Community Screening Interview for Dementia (CSID) is a reliable screening instrument for dementia, irrespe-ctive of educational level and cultural differences among various populations. This study aimed to develop the Korean version of CSID (CSID-K) for screening of dementia in Korean community residing old people. Methods : Study sample consisted of a dementia patient group and three control groups (depression, education, no education), each of them was composed of 30 community residing elders. CSID-K was administered, and demographic characteristic, depression, and cognitive function were investigated. Results : Split half reliability (Guttmans'), internal consistency (Cronbach's α ), and inter-rater and test-retest (two weeks) reliabilities of the CSID-K were excellent. Scores on the CSID-K were significantly correlated with other measures for cognitive function, In addition, scores on the CSID-K were significantly different between the dementia patient group and three control groups. Conclusion : CSI-K was a reliable and valid instrument to screen dementia in Korean community residing old people, It could be used not only in community settings but also in clinical settings and for cross-cultural researches.

HVPE법에 의한 후막 GaN의 선택 성장과 특성

김선태 한밭대학교 2004 한밭대학교 논문집 Vol.21 No.-

이 연구에서는 (0001) 사파이어 기판 위에 MOCVD법으로 성장된 GaN 박막 위에 SiO₂ 패턴을 형성한 후 HVPE법으로 후막 GaN를 선택 성장시켜 그 특성을 조사하였다. HVPE법에 의하여 선택 성장된 GaN의 결정성은 성장시간에 따른 두께 증가에 의해 개선되었으며, 이중 X-선 회절 반치폭은 두께에 대하여 지수 함수적으로 감소하여 최소치가 150 arcsec 정도이었다. 두께가 110 μm인 GaN에 대하여 11 K의 온도에서 측정한 광루미네센스 스펙트럼은 에너지갭 부근에서 잘 분리된 여기자 관련 발광과 얕은 준위의 도너-억셉터 쌍 사이의 발광으로 구성되었다. SiO₂ 마스크 위에 수평방향으로 성장된 영역에서는 MOCVD법으로 성장된 GaN 내에 존재하는 전위의 전파가 억제되어 결함밀도는 약 10^(7)cm^(-2) 정도이었다. In this work, we investigated some properties of selective area grown (SAG) GaN thick-films by hydride vapor phase epitaxy (HVPE) on the metalorganic chemical vapor deposition (MOCVD) grown thin-film GaN on sapphire substrate using SiO₂ pattern as mask. The crystal qualities of SAG-GaN was improved with the film thickness and the full width at half-maximum of double-crystal X-ray diffraction pattern for (0002) was exponentially decreased with thickness and reached a value of 150 arcsec. In a photoluminescence spectrum measured at 11 K for a 110 μm-thickness GaN, we can observed the well-defined free- and bound-excitonic emission bands and the shallow donor-acceptor pair emission band. The threading dislocations existing in the MOCVD grown GaN layer was effectively impeded by SiO₂ masks and the density of threading dislocation in the SAG-GaN grown by HVPE was estimated to an order of 10^(7)cm^(-2).

Diffusion of Zn into n-InP

김선태,박상보 大田工業大學 1990 한밭대학교 논문집 Vol.7 No.1

A systematic investigation on diffusion of Zn into InP has been performed using elemental Zn and P as source materials in sealed ampoule at temperature range of 550℃ to 700℃. Under well-defined diffusion conditions, the relation between diffusion depth with the amount of Zn and P in source and the diffusion time gives the good control of the diffusion depth. A high P-pressure source resulted in more desirable diffusion properties allowing a high planar fronts and diffusion-induced dislocation free layers.

발레,한국무용,일반학생간의 유산소 능력 및 체지방율 비교

조선자,김태운 釜山大學校 附設 體育科學硏究所 1991 體育科學硏究所 論文集 Vol.7 No.-

This study is to investigate the physiological influence of dancing on the body by comparision of %Fat and pulmonary function among three groups of college students-Sixteen ballet majoring students(Group A), thirteen Korean dance majors(Group B) and seventeen general stdents(Group C). A total of 46 freshmen and sophomores of P university were tested in view of, the Heart rate at rest, Vital capacity, Vo₂max, Vo₂max/wt, % Fat, Body Density. The results are as follows. 1. The heart rate at rest of Group A(69.9±6.8beats/min)and those of Group B(72.9±6.6beats/min) showed significantly lower statistics than those of Group C(80.2±9.4beats/min) 2. Vital capacity of Group A(3270.5±261.3㏄) and Group B(3048.3±310.9㏄) showed significantly higher statistics than those of group C(3025.9±312.7㏄) 3. Vo₂max was the highest among Group A(1.96±0.27ℓ/min), the second highest among Group B(1.78±0.27ℓ/min) and the lowest among Group C(1.74±0.15ℓ/min) 4. Vo₂max/wt of Group A and Group B was 38.00±5.92㎖/㎏/min(the highest) and 36.58±6.28㎖/㎏/min respectively. Vo₂max/wt of Group A showed meaningful difference from that of Group C.(32.61±5.31㎖/㎏/min) 5. % Fat of Group A and Group B was 20.30±3.57 %(the lowest), and 20.53±4.90%, respectively, which showed difference from that of Group C(23.63±3.09%) 6. Body density of Group A(1.0519±0.0086g/㎖) and Group B(1.0513±0.0117g/㎖) was meaningful higher than that of group C(1.0438±0.0117g/㎖)

말기환자 관리에 대한 의사들의 태도

최윤선,김장욱,신승욱,이영미,이태호,홍명호 가톨릭대학교 가톨릭대학교 간호대학 호스피스 교육연구소 1998 호스피스논집 Vol.3 No.-

Background : Nowadays, it is possible to prolong the life. And to improve the quality of life of the dying patients, they need to receive not only the physical, pscychological, social and spiritual care but also comprehensive and continuous care. Also, doctors have to consider many ethical issues especially in the dying patients. The purpose of this study is to analyze the doctors' attitude of those ethical issues and emphasize the importance of hospice. Methods : During September 1996, doctors who worked for a university hospital participated in this study by responding to the pre-made questionnaire. It dealt with the most suffering pain, the preferred death place, the use of life maintaining equipments, the application of cardiopulmonary resuscitation(CPR) to terminal patients and the doctors' experience and attitude euthanasia. Results : ① The most suffering symptom in dying patients was pain(60.0%). ② The death place was recommended by doctors was home(58.8%) and hospital (15.4%) in order. ③ 56.9% of doctors answered 'the life maintaining equipments isn't needed', 29.2% of doctors answered' according to the case', and 'needed' was 12.3%. ④ The application of CPR to the terminal patients, without the DNR chart, the only 15.4% of doctors answered "Do not CPR." But with the DNR chart, the percents of 'Do not CPR' was increased to 69.2%. The resident group did more CPR than specialist group(p<0.01). ⑤ The 50.8% of doctors agreed with the passive euthanasia. Conclusions : Many doctors follow the family member's opinion in the ethical issues of the dying patients. Euthanasia is forbidden by the law in Korea, but many doctors agree with the passive euthanasia. Hospice can solve the debating ethical issues including the euthanasia.

지역사회거주 노인에서 한국어판 세계보건기구 장애평가조사표의 개발

윤진상,김재민,신일선,양수진,정태길,이형영 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.1

Objectives : This study aimed to develop the Korean version of World Health Organization Disability Assessment Schedule II (WHODAS II-K) with community dwelling elderly population. Methods : The WHODAS II-K was administered to 1204 community residents aged 65 or over in two areas of Kwangju, South Korea, in 2001. For assessing 'health condition', data on physical illness, depression (Korean version of Geriatric Mental State Schedule B3), and cognitive dysfunction (Korean version of Mini-Mental State Examination) were collected. For evaluating 'contextual factors', informations on demographic charactehstics (age, gender, living area, marital state, and religiou), socio-economic status (education, type of accommodation, number of room, previous occupation, and current employment), and social network were obtained. Results : WHODAS II-K showed high levels of internal consistency, split-half reliability, and inter-rater and test-retest reliabilities. In the correlation analyses, scores on the WHODAS II-K were significantly correlated with the unfavorable conditions in the all variables on health condition and contextual factors. Partial correlations of scores on the WHODAS II-K with health condition were significant even after controlling for contextual factors. Conclusion : The WHODAS II-K is a reliable and valid instrument for assessing disability in elderly population since it reflects physical illness, depression, and cognitive impairment, which are common in elderly.

지역사회거주 노인에서 한국어판 세계보건기구 장애평가조사표의 개반

윤진상,김재민,신일선,양수진,정태길,이형영 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.1

유산양 유즙으로부터 분리된 세균의 분포 및 항균제 감수성 검사

김혜라 ( Hye Ra Kim1 ),정지영 ( Ji Young Jung ),김선득 ( Seon Deuk Kim ),박준영 ( Jun Young Park ),조인영 ( In Young Cho ),신성식 ( Sung Shik Shin ),손창호 ( Chang Ho Son ),오기석 ( Ki Seok Ok ),허태영 ( Tai Young Hur ),정영훈 ( Y 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4

The aim of this study was to isolate microorganisms from half milk samples of dairy goats by California mastitis test (CMT) during the lactation period and to further investigate the susceptibility of isolated organisms to antimicrobial drugs. From a total of 235 half milk samples with CMT scores of 2 or above from 366 dairy goats distributed throughout Jeonnam province, microorganisms were isolated from 198 (83.5%) samples either singly (99.0%) or in combination (1.0%). The most prevalent microorganism was the coagulase-negative Staphylococcus spp., (44.4%, n=88) followed by Staphylococcus aureus (24.2%, n=48), Escherichia coli (11.1%, n=22) and Streptococcus spp. (7.6%, n=15). Isolated bacteria also included Bacillus spp. (2.5%, n=5), Pseudomonas spp. (2.5%, n=5), Micrococcus spp. (1.5%, n=3), Corynebacterium spp. (1.5%, n=3), Enterococcus facium (1.0%, n=2), Morganella morganii (0.5%, n=1) and Streptococcus agalactiae (0.5%, n=1). During the summer season, a high prevalence of all micro-organisms were observed in which Staphylococcus spp. (30.8%), Escherichia coli (8.6%), and Streptococcus spp. (5.6%) were among the most prevalent bacteria isolated. Staphylococcus spp. was also shown to be high in the winter (21.7%). In most samples, the presence of bacterial pathogens in goat milk led to the increase in the total somatic cell count (SCC). Most of the half milk samples of dairy goats with bacterial contamination showed SCC of ≥1×10(6) cells/ml (90.4%). Minor pathogens (11.4%) were more detected from milk samples with SCC of <1×10(6) cells/ml than major pathogens (4.1%), while the major pathogens tended to be higher from samples with SCC of ≥3×10(6) cells/ml. Susceptibility of these bacteria to 12 antimicrobial agents was tested by the Kirby-Bauer disc diffusion method. Results indicated that more than 90% of bacteria isolated from CMT 2+ dairy goat half milk samples were susceptible to trimethoprim/sulfamethoxazole, amoxicillin/clavulanic, enrofloxacin and cephalothin while they were resistant to tetracycline (44.7%).