RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

Eurasian Otter (Lutra lutra) Habitat Suitability Modeling Using GIS; A case study on Soraksan National Park

Park, Chong-Hwa,Joo, Wooyeong,Seo, Chang-Wan Korea Spatial Information Society 2002 한국공간정보학회지 Vol.10 No.4

Eurasian otter (Lutra lutra) is one of endangered wildlife species whose population size is declining in Korea. To manage and conserve habitat for Eurasian otter, it is crucial to understand which habitat components affect otter habitat qualities. The objectives of this study were to develop a habitat suitability model of Eurasian otter in Soraksan National Park, to validate the model in Odaesan National Park. The research methods of this study were as follows. First, trace data and characters of Eurasian otter habitat were collected with Geographic Information System (GIS) data and Global Positioning System (GPS) receivers between 2000 and 2002. Second, the habitat use factors were identified as habitat characteristics of Eurasian otter and classified with habitat use and availability analyses. Third, significant factors of habitat model were extracted by Chi-square test. The last, Eurasian Otter Habitat Suitability Model (EOHSM) was employed by logistic regression method. Otter habitat use was positively associated with the reeds and shrubs areas adjacent to streams, the size of boulders, and low human disturbance in Soraksan National Park by EOHSM. This model had a classification accuracy of 74.4% at cutoff value of 0.5. Model validation showed a classification accuracy of 86.6 % at cut off value of 0.5 for otter habitat in Odaesan National Park.

덕유산 국립공원 등산로의 환경훼손에 대한 이용영향

서병수(Byung Soo Seo),김세천(Sei Cheon Kim),박종민(Chong Min Park),이창헌(Chang Heon Lee),이규완(Kyu Wan Lee) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.3

The object of this study was to examine the user`s impacts on the environmental deteriorations of trail at Ticket Office - Paekryunsa (Temple) Hyangcho^kpong - Dongyupryung - Chilyun Fall area in To^kyusan National Park. Four trails were sampled in the study area according to the amount of users. Then the user`s impacts on trail were measured at each trail. The Ticket Office-Paekryunsa trail was the mo;;t used district and followed at Paekryunsa-Hyangcho^kpong trail, Hyangcho^kpong-Dongyupryung trail in descending order. Dongyupryung-Chilyun Fall trail is not used by people because of rest rotation system. The entire width of trail was greater at the more heavily used trail. Maximum depth, cross-sectional area loss, and surface texture and roughness of trail were the highest at Paekryunsa-Hyangcho^kpong trail. Soil hardness, soil acidity, soil moisture content, organic matter content, and exchange canon were influenced by trampling. Soil hardness, soil acidity and exchange canon increased in tramples soil, but content of soil moisture and organic matter decreased therein. Environmental deteriorations of trail were significantly influenced by the amount of users and the slope of trail. Bared lands about 2.000㎡ were appeared by trampling and camping around Hyangcho^kpong. Effects of carrying of rest rotation system for National Park were partly recognized at Dongyupryung-Chilyun Fall trail.

Distribution of the Fairy Pitta (Pitta nympha) in the South Korea: A focus on protected areas

Gil-Pyo Hong(홍길표),Jae-Hoon Kim(김재훈),Seul-Gi Seo(서슬기),So-Yeon Cho(조소연),Bo-Yeon Hwang(황보연),Jong-Hyun Park(박종현),Seung-Yeon Lee(이승연),Ga-Young Kim(김가영),Wan-Hee Nam(남완희),Ha-Cheol Sung(성하철) 한국조류학회 2021 한국조류학회지 Vol.28 No.2

국내 팔색조(Pitta nympha) 분포 현황을 알아보고자 전국 국립공원 등 보호지역 중심으로 총 19개 지역을 조사하였고, 전문가 네트워크를 통해 자료를 보강하였다. 전국 19개 지역을 조사한 결과 북한산, 지리산 등 내륙지역 7개 국립공원에서는 팔색조가 확인되지 않았고, 경남 거제, 남해, 해남, 장흥 고흥 등 남해안과 인접한 12개 지역에서 팔색조 서식이 확인되었다. 또한 전문가 네트워크를 통해 수집한 팔색조 출현 위치 정보 82건을 분석한 결과, 전국 8개 광역지자체 모두 팔색조 서식이 확인되었고, 내륙지역(26.8%)보다 해안지역(73.2%)에서 더 많이 출현하였다. 연구 결과를 종합해보면, 우리나라의 팔색조는 주로 제주도와 남해안 지역을 중심으로 많이 분포하며, 그 보다 적은 개체수가 내륙 지역에 넓게 분포하고 있는 것으로 나타났다. The present study investigated the current distribution of Fairy Pitta (Pitta nympha) in South Korea. A total of 19 study areas, including protected areas such as national and provincial parks in South Korea were surveyed, and additional data obtained from an expert network. According to the results, Fairy Pitta was absent in seven inland national parks, including Mt. Bukhan and Chiri parks. Fairy Pitta inhabited 12 areas near the southern coast of South Korea, including Geoje, Namhae, Haenam, Jangheung, Goheung, and Gyeongnam Province. The analysis results of 82 datasets obtained from experts with regard to the locations where Fairy Pitta have been spotted showed that the bird inhabits all the eight metropolitan regions in South Korea, and is more frequently spotted in coastal areas (73.2%) than in inland areas (26.8%). The findings demonstrate that Fairy Pitta is mainly distributed in Jeju Island and the southern coast of South Korea, and is distributed in inland areas at relatively lower numbers.

보호지역 경계조정을 위한 공간의사결정지원모델 연구 -지리산 국립공원을 사례로-

성혜정 ( Hye Jung Sung ),권혁수 ( Hyuk Soo Kwon ),서창완 ( Chang Wan Seo ),박종화 ( Chong Hwa Park ) 한국환경복원기술학회 2011 한국환경복원기술학회지 Vol.14 No.3

The purpose of this study are to develop a SDSS (Spatial Decision Support System) that can incorporate diverse opinions of stakeholders related the designation of protected areas (PA), and to employ the model for the readjustment of the boundary line of the Jirisan National Park of Korea. The SDSS would lead to more rational and less controversial decision-making during the expansion or removal of PA in Korea. Research methods are as follows. Firstly, to select evaluation criteria for SDSS for PA designation by using expert interview and literature survey. Secondly, to measure their preferences on the designation of additional PA or the removal of a part of PA based on the opinions of various stakeholders such as local residents, environmental groups, or public officials. Thirdly, to produce conservation priority maps based on a multi-criteria decision making technique. The SDSS would be used to rational decision making for the expansion of PA or the release of a certain part of PA by reflecting diverse preferences on biodiversity conservation and economic interest of residents. The visualization of conservation priority maps would also increase the efficiency of such decision making processes. The evaluation criteria for the expansion of PA for biodiversity conservation includes vegetation conservation value, wildlife conservation value, and the habitats of key species. The evaluation criteria for the removal of PA includes the proximity to roads and the boundary of PA, land use types, and conservation zoning of the PA. Preference weights are based on data collected from the Jirisan National Park. Both the conservation priority and removal priority maps are based on land parcels so that property rights of all parcels would be correctly represented.

두개강내 출혈을 동반한 불응성 특발성 혈소판감소성 자반증의 CYCLOPHOSPHAMIDE 치험 1예

서정철,양태영,최연수,박유환,김완중,허경,정원영,정춘해 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Idiopathic thrombocytopenic purpura(ITP) is an autoimmune bleeding disorder characterized by the development of antibodies to one's own platelets, which are then destroyed by phagocytosis in the spleen and liver. Its clinical manifestations are characterized by bleeding and the most serious complication is hemorrhage into the central nervous system, but which is rarely seen below 1%. In treating IPT, the therapy of first chice is administration of adrenocorticosteroid and r-globuline. If satisfactory results are not obtained, then splenectomy is recommended. If this also proves inadequate, then administration of immunosuppressants is carried out. 16-year-old male was admitted with complaints of left hemiplegia and nasal bleeding. This case may be developed spontaneous intracerebral hemorrhage causing thrombocytopenia and was treated by steroid, r-globuline and splenectomy, but satisfactory results are not obtained. After all, the paient was successfully treated with cyclophosphamide.

강원도 인제군 원대리 자연습지의 식물상

박완근,유석인,박광서 강원대학교 삼림과학연구소 2000 Journal of Forest Science Vol.16 No.-

강원도 인제군 남면 원대리 자연습지를 대상으로 1999년 4월부터 동년 10월까지 관속식물상을 조사한 결과 71과 175속 228종 1아종 33변종 3품종, 총 265종류가 분포하는 것으로 나타났으며, 이 가운데 갈대, 달뿌리풀, 삿갓사초, 골풀, 구릿대, 버드나무 등의 수생 및 관속식물은 19과 24속 31종 5변종 1품종, 총 37종류가 분포하고 있었다. 본 조사지역내 식생의 특성을 파악하기 위해 식물사회학적조사를 실시한 결과 갈대군락, 갯버들-횐물봉선아군락, 조팝나무-사철쑥아군락으로 분류되었다. This study was to estabilish the composition and vegetation of vascular plants on natural mashes in Wondae-ri(710m; 37˚ 58' 30" N, 128˚ 12' 15" E). This work was conducted from April to October, 1999. Vascular plants were composed of 71 families, 175 genera, 228 species, 1 subspecies, 33 varieties and 3 formae, totaling 265 taxa. Among the investigated vascular plants, 19 families, 24 genera, 31 species, 5 varieties 1 formae, totaling 37 taxa(13.9%) were hydrophytes and hygrophytes. The forest vegetation was classified Into 1 community and 2 subcommunities ; A.Phragmites japonica community A-1.Salix gracilistyla-Impatiens noli-tangere subcommunity A-2.Spiraea prunifolia var. simpliciflora-Artemisia capillaries subcommunity Key words : natural mashes, hydrophytes and hygrophytes

타리비드 정(오플록사신 100㎎)에 대한 파비드 정의 생물학적동등성

박완수,조성희,이헌우,임호택,홍성제,서성훈,류재환,이경태 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.1

The purpose of the present study was designed to evaluate the bioequivalence of two ofloxacin tablets, Tarivid (Jell Pharm. Co., Ltd.) and Favid (ILHWA Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-four normal male volunteers, 23.67±3.12 year in age and 68.50±7.23 kg in body weight, were divided into two groups and a randomized 2x2 cross-over study was employed. After four tablets containing 100 mg of ofloxacin were orally administered, blood was taken at predetermined time intervals and concentrations of ofloxacin in plasma were deter mined using HPLC. Pharmacokinetic parameters such as AUC_(t) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t), and C_(max) and untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transformed data were acceptance range of log 0.8 to log 1.25 (e.g., log 0.94-log 1.04 and log 0.90-log 1.07 for AUC_(t) and C_(max) respectively). The major parameters. AUC_(t) and C_(max) met the criteria of KDFA for bioequivalence indicating that Favid tablet is bioequivalent to Tarivid tablet.

Swanson의 주의력결핍 장애척도의 한국판 표준화

이종범,박성찬,정성덕,김진성,서완석,배대석,주열 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.2

연구목적 : 한국판 Swanson의 주의력 결핍 장애 척도(이하 SNAP)의 신뢰도와 타당도를 검증하고, 이를 국내 실정에서 주의력결핍 과잉행동장애 환자를 선별할 수 있는 평가용 도구로서 적합한지 여부와 연구에 사용할 수 있는지를 알아보고자 하였다. 방 법 : 1998년 7월 1일부터 2001년 9월 30일까지 영남대학교 의료원 신경정신과 외래를 방문한 주의력결핍 과잉행동장애 환자 392명과 정상대조군 432명을 대상으로 SNAP을 시행하였다. 결 과 : 남녀 간의 SNAP 척도 점수비교에서, 환자군에서는 유의한 차이가 없었으나 정상 대조군에서는 남자가 유의하게 높게 나타났다(p<.01 ∼ .001). 검사-재검사 신뢰도 검증에서 신뢰도 계수는 각 하위 척도에서 .85에서 .92로 모두 만족할 만큼 높았다(p<.05). 내적일관성에 의한 신뢰도 검증에서 정상 및 환자군에서 각 하위척도별로 Cronbach α값이 .80(p.05)이상으로 나타났고, 반분신뢰도는 정상 및 환자군에서 각 하위척도별로 0.731에서 .866사이였다(p<.05). 주의결핍장애 평가 척도-가정관(Attention Deficit Disorder Evaluation Scale-Home Version) 및 소아기 집중력 문제 척도(Childhood Attentin Problem Scale)와의 준거 타당도는 ADHD 환자군에서 .342에서 .713사이였고(p<.05), 정상 대조군에서 .551에서 .789였다(p<.05). 요인분석을 해 본 결과 본 척도는 3개의 요인으로 분류되었는데, 부주의(inattention) 요인, 충동성/과잉행동(impulsivity/hyperactivity)요인, 또래 관계(peer interaction)요인으로 구분되었다. 본 척도가 정상인과 환자를 감별할 수 있는 척도인가를 알아보기 위한 감별 타당도 검사를 해본 결과 87.5%를 분류하여 높게 나타났다. 환자와 정상인을 구분하는 점수인 절단점은 표준편차 1.5를 절단점으로 잡을 때 이는 백분율로 93percentile에 해당하는 점수로서 각 소척도 점수에서 남자(0.98∼1.65)와 여자(0.64∼1.38)의 절단점이 다르게 나타났으며 남자가 높게 나타났다. 결 론 : 이상의 결과에서 볼 때 한국판 SNAP 평가 척도의 신뢰도와 타당도가 입증되었고 환자를 판별하는 능력이 우수하여 향후 ADHD 연구에 유용한 도구로 사료된다. Objectives : The purpose of this study was to examine reliability and validity of Swanson's attention deficit disorder sacale(SNAP). Method : SNAP was administered to 432 elementary school students and 392 patients who were diagnosed as attention deficit hyperactivity disorder in Yeungnam university hospital from 1998 July to 2001 September. Results : In the reliability test, the test-retest reliability coefficient was significantly high and that of inattention was 0.85, that of impulsivity was 0.90, that of hyperactivity was 0.90 and that of peer interaction was 0.92(p<.05). In the reliability test by internal consistency, the Cronbach α coefficient was above 0.80 at each subscale in both patients and normal control group(p<.05). The split-half reliability coefficient by first-second half method showed high correlation and those of each scales were from 0.731 to 0.866 in both patients and normal control group(p<.05). The criterion validity of SNAP with Attention Deficit Disorder Evaluation Scale-Home Version(ADDES-HA) and Childhood Attention Problem Scale(CAP) scale was from 0.342 to 0.713 in patients group, from 0.551 to 0.789 in normal control group(p<0.05). The discriminant ability between ADHD and normal subjects by SNAP was 87.5%. Conclusion : In this point of view, SNAP scale showed high reliability and validity in applying to Korean subjects and was proved to be useful screening tool for attention deficit hyperactivity disorder reserch.

코자 정(로자탄 칼륨 50mg)에 대한 로자르탄 정의 생물학적 동등성

김용원,박완수,김성수,서지형,조성희,이헌우,류재환,이경태 한국약제학회 2006 Journal of Pharmaceutical Investigation Vol.36 No.2