Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

계분으로부터 살모넬라에 감염된 가금류의 신속한 진단

박홍양 동물자원연구센터 1999 1999년도 동물자원연구센터 학술심포지움 Vol.1999 No.-

우리의 건강과 깊은 관계가 있는 유해미생물중 하나인 살모넬라균은 최근 심각한 사회문제로 대두되고 있다. 가금류인 닭의 경우 살모넬라가 주로 장내에 번식하고 있어 도살시 계육뿐만 아니라 계분과 함께 배출되어 산란된 난에까지 오염이 이어지고 있다. 더불어 육계, 산란계로부터 조기에 살모넬라 감염여부를 파악하는 것이 중요한데 현재의 검출법 중 하나인 단순배양법의 경우 시간이 오래걸리고 많은 인력과 비용이 드는 단점이 있다. 그래서 본 연구는 살모넬라에 감염된 닭의 분을 이용해 유전공학기법의 하나인 PCR(Polymerase Chain Reaction)을 이용해서 살모넬라균 특유의 유전자의 염기 서열을 찾아 검출에 필요한 primer(S-1,S-2,S-7,S-8)를 제작하고, 이를 적절하나 조건(94 ℃에서 30초간의 denaturation, 63 ℃에서 20초간 annealing, 72 ℃에서 40초간의 extension 과정을 30번 반복)에 맞추어 실험하여 닭의 도축전에 감염여부를 신속하게 판단하여 양계농가의 경제적 이익과 국민의 건강증진에 초점을 두고 있다.

Mulligan 치료법과 고유수용성 신경근 촉진법이 동결견 환자의 치료에 미친 효과

양정애,윤홍일,박현식,신영일,전범수 대한정형도수치료학회 2006 대한정형도수물리치료학회지 Vol.12 No.1

The purpose of this study was to compare the effects of Mulligan method and PNF method on the pain and limitation of range of motion in patients with frozen shoulder. The subjects of this study were 20 patients, 10(50%) males and 10(50%) females. They visited clinic for physical treatment within 6 months after onset of shoulder pain and limitation of range of motion with frozen shoulder. One group was applied with Mulligan method and other group was with PNF method . The patient were treated 5 times session weekly for 6 weeks from March 4th, 2002. And each treatment session was 15min. with physiotherapy. The pain was measured by visual analogue scale (VAS) and rage of motion (ROM) of flexion, abduction, external rotation, internal rotation were measured by goniometer. The data was analysed by paired T-test and independent T-test. The results of this study were summarized as follow : 1. The ROM of Mulligan method group increase in after treatment in comparison with ROM in before treatment, it is significant increase. Although the VAS of MMG decrease in before than after treatment, it is significant difference. 2. There is significant difference in before and after treatment of ROM of shoulder flexion, abduction, internal rotation, external rotation between PNF method group. The VAS of PMG is decreasing in before than after treatment, it is significant difference. 3. There is significant difference in before movement and after movement ROM of flexion, abduction, internal rotation, external rotation and VAS between Mulligan method session and PNF method session then the scale which measured by Mulligan method was more increased than that of PNF method. The results showed that both Mulligan method and PNF method were effective in pain reduce and ROM increase, but Mulligan method was superior to PNF method in ROM increase and pain reduce.

치과용 인상재의 점성에 관한 연구

박영준,김강연,양홍서 大韓齒科器材學會 1994 대한치과재료학회지 Vol.21 No.1

The purpose of this study was to investigate the rheological properties of a syringe type agar impression material, a zinc oxide eugenol impression paste and 6 types of elastomeric impression materials. Boiled agar impression material was cooled from 80℃ to 32℃, and its viscosity was measured during the temperature decrease at three different shearing rates. Viscosities of unmixed impression pastes were measured at three different temperature conditions and three different shearing rates. Impression materials were mixed at 23 and 30℃, and the viscosities were measured as a function of elapsed time from mixing. At 30℃ mixing condition, viscosities of condensation silicone rubber impression materials were measured with the variation of base/catalyst ratio, and viscosities of addition silicone rubber impression material with the varying amounts of retarder were measured. The results are as follow; 1. In all impression materials, viscosity values were decreased as the shear rate increased(P<0.05). 2. Viscosity of agar impression material was increased rapidly when the temperature decreased below 42℃(P<0.05). 3. Viscosities of unmixed impression materials were decreased by the increased temperature. Condensation and addition silicone rubber impression materials showed low viscosities, and polysulfide impression materials showed moderate viscosities, and polyether and zinc oxide eugenol impression materials showed much higher viscosities than other impression materials. 4. As the room temperature increased, the mixed impression materials showed significantly rapid increase of viscosities during the same lapse of time(P<0.05). Polysulfide rubber and zinc oxide eugenol impression materials retained low viscosities for longer time in the course of setting. Ramitec, polyether rubber for bite registration, attained high viscosity within short elapsed time. On the other hand, Impregum-F polyether rubber impression material showed slowed slower increase of viscosity than condensation and addition silicone rubber impression materials. 5. Viscosities of condensation silicone rubber impression material reacted sensitively with the variation of base/catalyst ratio(P<0.05). 6. Addition silicone rubber impression material mixed with 2 drops of retarder per 5 cm pastes showed no significant difference in viscosity compared with normal mixing condition(P>0.05), but 4 drop-added mix showed significant decrease in viscosity compared with normal mixing group(P<0.05).

교류전해에칭용 에틸렌글리콜의 알루미늄박판의 부식억제 효과

박성민, 양정진, 김홍일, 김한주, 김성한, 신진식, 박수길 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1

In this study, additions of aluminium foil eching characteristics were compared with sulfuric acid for capacitor. sulfuric acid and ethylene glycol is used as addition, characteristics of funtional group from addition were guessed by comparing of aluminium foil eching characteristics. When sulfuric acid was added in HCI etching solution, corrosion of aluminum metal was inhibited by the chemical adsorption of sulfate ion, when ethylen glycol was added in HCI etching solution, corrosion inhibited by the chemical adsorption of OH­^ion.

배차계획을 위한 대화형 의사결정지원시스템

박양병,홍성철 한국경영과학회 1998 經營 科學 Vol.15 No.2

Truck dispatching is one of the most commonly occurring problems of transport management. We developed an interactive decision support system named IDSSTD, for the truck dispatching problem where two conflicting objectives are treated and travel speed varies depending on the passing areas and time of day. The IDSSTD aids the decision-making process by allowing the user to interact directly the database, to direct data to a decision model, and to portray results in a convenient form. The IDSSTD is consisted of two major interactive phases. The pre-scheduling interactive phase is to reduce the complexity of a given problem before applying the BC-saving heuristic algorithm and the post-scheduling interactive phase is to improve practically the algorithmic solution. The IDSSTD has the capabilities of its own manipulation(analysis and recommendation) and diverse graphic features in order to facilitate a user's interaction.

말초성 신경아세포종 : 두개골 발생 증례 보고 Involving Skull

박양화,권익승,류연석,신미경,최문배,홍승관,문명선 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.4

The peripheral neuroblastomas are malignant neoplasms that originate from the adrenal gland and sympathetic nervous tissues. They usually occur in childhood. They exhibit such malignant features that they metastasize early to lung, liver, bone, rarely skull and other structures. The patients with peripheral neuroblastoma have short duration of symptom and short survival period. They reveal the characteristic light microscopic features that resemble other small cell carcinomas. The establishment of final diagnosis of peripheral neuroblastoma needs various special stainings for small cell carcinomas, and the electron microscopic findings are the most reliable. The authers recently experienced a case of peripheral neuroblastoma in a 26-year old man which involved right frontoparietal skull vault. The only chief complaint was a local non-tender mass at right frontoparietal scalp. Neither headache nor any neurological deficits was detected. Simple skull X-ary revealed a punched out rediolucency at right frontoparietal bone and brain CT showed a superficial elliptical high density mass that enhanced strongly. The mass was totally removed by wide craniectomy. The tumor invaded and penetrated the dura but the arachnoid membrane. at the tumor bed was not invaded by the tumor. The tumor was confirmed as peripheral neuroblastoma by various special stainings for small cell carcinomas. Following surgical resection of the mass, post-operative radiotherpy was offered(4800 rads for about 5 weeks). The patient aggrevated progressively and showed numerous metastases to such bones as lumbar vertebrae, pelvis and humerus to became paraplegic. 8 months after the operation, the patient died.

폐결핵 환자의 우울 상태

홍정표,최선희,조만종,이성주,박종,김기순,김양옥 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1

The main purpose of this study is to identify and evaluate the depressive states for the patients with chronic pulmonary tuberculosis. A questionnaire(Self-rating Depression Scale, SDS) survey was performed toward 57 pulmonary tuberculosis patients with initial treatment who started chemotherapy and 53 pulmonary tuberculosis patients with retreatment, admitted to one tuberculosis-centered hospital and two general hospital. Fifty three healthy persons as control group were investigated with the same questionnaire for comparison. The survey was performed from July to August 1996. The results are as follws : 1. The patients had significantly high scores in the depression than control and the patient with retreatment showed significantly high scores in the depression than the patient with initia1treatment(P<0.01). 2. The depressive levels of the patients were associated with duration of admission and the expectancy of recovery (P<0.05). 3. The percentage of the subjects with 40 or more scores tentatively classifiable as psychologically depressed was 67.1% of the total subjects, 48.4% of the control groups, 75.0% of the patient with initial treatment and 84.1% of the patient with retreatment. Because patient with pulmonary tuberculosis with initial treatment showed higher depression scores than normal persons, and patients with retreatment due to failure of initial treatment showed higher depression scores than initial treatment, it is necessary to give attention to depression of the patients during treatment of pulmonary tuberculosis.

Opaque의 두께가 전장용 레진의 색채에 미치는 영향

박상원,조신석,양홍서 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.3

The purpose of this study was to evaluate the color difference of specimens with the variation of metal type and opaque thickness. Resin veneered specimens with the same color were fabricated and evaluated by the CIE L*a*b* system. Specimens were divided into 4 groups as follows ; Group P1; Resin with precious metal alloy, 0.1 mm opaque layer Group P2; Resin with precious metal alloy, 0.5 mm opaque layer Group B1; Resin with nonprecious metal alloy, 0.1 mm opaque layer Group B2; Resin with nonprecious metal alloy, 0.5 mm opaque layer The results obtained were as follows ; 1. L* value of group P1 was lower than that of group P2. There was no significant differences in a* values among tested group. 2. Opaque thickness did not produce any significant differences of L*, a*, b* value in the resin veneer groups on the base metal. 3. The ΔE*ab value between P1 and P2 was 2.228 which is noticeable, while the color difference between B1 and B2 was slight.

전신마취중 에피네프린사용으로 발생되는 부정맥

박선규,진홍용,양홍석 중앙대부설한국의과학연구소 1985 한국의과학 Vol.17 No.3