pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

미토콘드리아 DNA 염기서열 분석법에 의한 가족관계의 규명

남용석,이희석,김희선,이혜린,황적준 大韓法醫學會 1995 대한법의학회지 Vol.19 No.1

The human mitochondrial DNA has two characteristics that make it possible to identify individuals and establish family relationships. First, it is haploid, being exhibited only maternal inheritance. Second, it is highly variable on the hypervariable control region of mitochondrial DNA. Taking advantage of two characteristics of mitochondrial DNA, individual relationships in dispute were identified by combining PCR amplification with direct mitochondrial DNA sequencing. Two persons who alleged the same maternal lineage were identical on the mitochondrial DNA sequences from 15,960 to 16,569 and from 1 to 533. Other two persons were identical on the mitochondrial DNA sequences from 16221 to 16390 each other. However, seven bases are different on the sequences from 16221 to 16390 between two groups,. Even though four persons are kinship, these results suggest that they should come from two different maternal lineage.

삼풍백화점 붕괴사고 희생자들의 신원확인을 위한 유전자검사

남용석,이혜린,김경훈,김희선,이희석,황적준 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

A DNA typing was performed to identify decomposed body remains from Sampoong Department mass disaster in June 1995. These body parts include bone fragment, skin tissue, hairs, from which the extracted DNAs were highly degraded. Two VNTR loci, 4STR loci, and amelogenin gene were chosen for AMP-FLP, and mtDNA sequence analysis for the confirmation of maternal relationship. The results of AMP-FLP of the selected polymorphic loci showed different sucess rate for PCR. DIS80 and D17S5 loci were amplified successfully form 64.5%, and 67% of the samples, respectively. HUMTHOI, HUMCSF1PO, and HUMTPOX loci were amplified successfully from 90.3% of the samples each. HUMACTBP2 and amelogenin was amplified in 87% of the cases submitted. THE DNA types of 33 remains were compared with those of 81 bereaved families consisting of 173 member. Thirty three samples were reduced to 28 in numbers according to results of the same DNA types. Among them, the DNA types of 15 remains matched with those of bereaved families and the identified remains were reconfirmed by amelogenin sex typing and mitochondrial DNA sequence analysis. The others were not identified a family by failures of PCR amplification or non-matching of DNA types. Also it is confirmed that one hair sample should be artificial by non-digestion of protease and another be animal bone by result of dot blotting with human Alu probe. Our results indicate that multiplex PCR system consisting of several STR loci like HUMCSF1PO, HUMTPOX, and HUMTHO1 is more effective for the identification of highly decomposed human remains from mass disaster.

韓國人의 HLA DQA1 遺傳座位에 대한 集團 遺傳學的 特性

남용석,김희선,이희석,이혜린,황적준 大韓法醫學會 1995 대한법의학회지 Vol.19 No.1

Using reverse dot blotting technique, genotype of HLA DQAl locus have been determined from 142 unrelated Korean individuals. Twenty genotypes were found from possible twenty one genotypes - the missing one was A2/A2 that had lowest expected frequency. All of known 6 alleles were found with each of its frequency being 15.1% for A1. 1, 16.6%for A1.2, 12.7% for A1.3, 11.6% for A2, 25.7% for A3 and 18.3% for A4. After X?-test(p>0.1), G-test(p>0.05), and by comparision of expected (0.82) and observed heterozygosity(0.81), the population was confirmed to be on the state of Hardy-Weinberg equilibrium. The gene diversity(0.82) of Korean population, which generally thought to be a group of single unity, actually was higher than that of most other populations. The pattern of alelic distribution was different from that of other populations, especially allele A1.3 which displayed heterogeneity between other goups with significance(p<0.01), as it turned out to have anthropological significance. After all, this HLA DQA1 system, even though its small number of alleles, having high degree of heterozygosity, was proven to be effective in individual identification, and paternity testing in Koean population.

담수표면 직파재배 펠렛종자의 파종 및 초기생육 특성

김석언,유수남,김용재,박태동,이인,최영수 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

In spite of labor saving in rice cultivation, the main factors preventing the enlargement of the direct wet seeding culture are unstable seedling establishment and greater susceptibility to lodging. In order to solve these problems, developed rice-seed pellets were investigated on sowing and early growth characteristics of seed pellet. Seed shifting, flooding seedling, and lodging of seeding percentage of pellet seed were lover than pregerminated or dry seed. Soil thickness covering seed of pellet seed was 2 ㎜ and shifting width of seed of that was 1.5 ㎝. Establishment percentage of pellet seed was the highest in 0 ㎝ of seeding depth. Early plant height, root length, number of leaf and root of pellet seed with seeding time were similar to those of dry seed.

소아의 상완골 원위부 과간 골절

이우석,정환용,김우식,김용찬,전택수,김남현,김규태 대한골절학회 2004 대한골절학회지 Vol.17 No.4

상완골 원위부 과간 골절은 소아에서는 매우 드물게 발생되는 골절로 대부분 주관절의 굴곡상태로 주관절 후방부에 직접적인 충격에 의해 발생하는 것으로 보고되고 있다. 전위가 없거나 경미한 경우 도수적 정복술 혹은 도수적 정복술 후 경피적 핀고정술을 시행하며, 전위가 심한 골절이거나 도수적 정복술이 불가능한 경우 관혈적 정복술 후 핀고정술을 시행하는 것으로 알려져 있다. 저자들은 전위된 상완골 원위부 과간 골절 환아 2예에서 각각 도수적 정복술과 관혈적 정복술 후 K 강선 고정술을 시행하여 만족한 결과를 얻었기에 보고하는 바이다. Intercondylar fractures of the distal humerus are very rare in children. The pattern of the fracture would suggest that the mechanism of injury involves a fall directly on the flexed elbow. Most agree that the undisplaced fracture can be managed conservatively. With increasing displacement and comminution, the opinions tend to differ. We suggest that closed reduction with percutaneous K wire pinning and open reduction with percutaneous K wire pinning offer a satisfactory methods by which to treat displaced intercondylar fracture in children.

쇄골 골절에서 지연성 상완 신경총 마비 : 1예 보고 A Case Report

이우석,정환용,전택수,김용상,김남현 대한골절학회 2003 대한골절학회지 Vol.16 No.2

상완 신경총 마비는 쇄골 골절 후에 매우 드물게 발생하며, 대부분 많은 가골 형성을 동반한 쇄골의 중간1/3 부위 골절 불유합에서 발생한다. 골절의 치유 과정에서 과형성된 가골이 상완 신경총을 압박할 수 있으며, 감압술고 불유합에 대한 수술적 치료를 필요로 한다. 이에 저자들은 쇄골 골절 후 불유합과 과도한 가골 형성에 의해 발생된 지연성 상완 신경총 마비 환자를 치험 하였기에 보고하는 바이다. The brachial plexus palsies secondary to nonunion of the clavicle fracture are extremely rare. The nonunions are hypertrophic and usually in the middle third of the clavicle. Hypertrophic callus produced healing process will cause a compression of the neurovascular bundle. This lesion requires operative treatment for decompression of the brachial plexus and internal fixation of nonunion. We present a case of delayed brachial plexus palsy due to nonunion and excessive callus formation of a clavicular fracture.

구강에서 분리한 E.durans의 S.mutans와 S.oralis에 대한 작용

김용남,양규호,오종석,정진 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.2

장구균은 사람의 구강, 질, 장내 등에 정상적으로 존재하는 세균이다. 본 연구에서는 구강에서 분리된 장구균중 Enterococcus durans로 동정된 분리균주 4주의 특성과 구강내 주요 세균인 Streptococcus mutans와 Streptococcus oralis와의 관계를 연구하여 다음과 같은 결과를 얻었다. 1.분리균주에 대한 당 발효 검사와 생화학적 검사 결과, 비슷한 양상을 보였다. 2.분리균주 모두 erythromycin, penicillin, tobramycin, ampicillin, teicoplanin, ciprofloxacin, vancomycin, gentamicin, kanamycin 및 streptomycin에 대해 감수성을 보였다. 3.S.mutans를 일회용 큐벧에서 단독 배양시 550 nm에서의 흡광도가 1.405이었으나, S.mutans와 4주의 분리균주 혼합 배양시에는 각각의 흡광도가 0.855, 0.867, 0.797, 1.083으로 감소되었다. 4.비커 와이어 검사 결과, S.mutans 단독 배양시 형성된 인공치태의 평균 무게는 1566 ±103 mg이었다. S.mutans와 4주의 분리균주 혼합 배양시에는 각각 44 ±5 mg, 41 ±12mg, 34 ±7mg, 38 ±12mg으로 현저히 감소되었다. 배양후 생균수는 S.mutans 단독 배양시 ml당 2.0 ×10□이었으나, S.mutans와 4주의 분리균주 혼합 배양시 S.mutans는 2.0 ×10□내지 6.0 ×10□으로 감소되었다. 5.S.oralis 단독 배양시 ml당 2.1 ×10□이었으나, S.oralis와 4주의 분리균주 혼합 배양시 S.oralis는 1.4 ×10□내지 7.0 ×10□으로 감소되었다. 6.3주의 분리균주로 부터 약 60 kb의 plasmid를 분리할 수 있었다. 이상의 결과를 종합하면 구강에서 분리된 E.durans는 S.mutans의 증식을 억제하여 인공치태 형성을 저지하였고, S.oralis의 증식은 약간 억제하였다. 주요어 : Enterococcus durans, Streptococcus mutans, Streptococcus oralis

당뇨병 유발쥐혈청내 methylated amino acid의 함량 변화

김용기,유세근,남석우,이향우 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1

In order to investigate the relationship between methylated amino acids and diabetes, we investigated the changes of serum concentration of methylated amino acids in serum of the experimentally induced diabetic rats. The diabetic symptom was induced by injectly alloxan 100 mg/kg intraperitoneally. The serum content of N^G-monomethylarginine(MMA), N^G, N^G-dimethyl-arginine(DMA) and N^G, N^G-dimethylarginine(D'MA) were analyzed by HPLC. The results are as followings ① There was no change in serum content of MMA in the diabetic group. ② But it was observed that the amounts of DMA and D'MA were increased remarkably in the diabetic group. Specifically, D'MA content was increased about 7 times in comparision to that of the control group. ③ However, the concentration of trimethyllysine in RBC was decreased slightly in diabetic group but not significant statistically.

스테인리스 鋼纖維 强化알루미늄 基地金屬 複合材料의 界面反應에 관하여

金錫胤,南勝義,朴忠河,玄昌容 弘益大學校 1987 弘大論叢 Vol.19 No.2

This study is based an the reaction of the Interface stainless steel and aluminium to which specific elements were added. The aluminium and aluminium alloy which had been reinforced with stainless steel fiber was made by the liquid infiltration method an the researchs. When the composite material was made, cobalt was appeared but the other elements didn't appear at the interface. In the microscopic examination, the thickness of each Interface didn't change and the diameter of the fiber was reduced. Most of the composite material had been higher strength than theoretical value, which establishs the fact that the Interface cohesion is good when the composite materials are made.