단백 methylase 억제제 5´-Isobutyl-5´-deoxyadenosine과 sinefungin에 의한 BALB/c mouse 비장세포의 증식과 분화의 억제

최의규,홍정희,한유정,백문기 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

Whether or not protein methylation can be correlated with cell proliferation and differentiation in BALB/c mouse splenocyte cultures has been investigated by using methylase inhibitors, 5'-Isobutyl-5'- deoxyadenosine(SIBA) and sinefungin. 1) Protein methylase I and III activities were found to be elevated in LPS stimulated splenocytes, and the peak of protein methylase I was correlated with that of S phase fraction while changes in protein methylase III activity followed the changes in S phase fraction. 2) SIBA and sinefungin were proved to be potent competitive inhibitors of the protein methylase 'I and III in vitro with apparent Ki for sinefungin of both enzymes being lower than that for SIBA. 3) The protein methylase III were activated by 2-mercaptoethanol and dithiotreitol, and strongly inhibited by Cue+ in vitro; and the inhibition was almost completely released by dithiotreitol and diethyldithiocarbamate. 4) The extent of polyclonal antibody formation, S phase fraction and protein synthesis after induction by LPS were significantly inhibited in a dose response manner by SIBA and sinefungin with higher potency for the former. 5) Inhibition of cell cycle was also observed in the presence of cyloleucine, an inhibitor in SAM synthesis. 6) Of several enzymes investigated, namely protein methylase III, LDH, yglutamyltranspeptidase, and adenosine deaminase, the- protein methylase III was most markedly increased by LPS and decreased by SIBA and sinefungin than other enzymes. From the above results, it is suggested that SIBA and sinefungin suppress the cell proliferation and differentiation through the inhibition of protein methylation.

기하광학영역에서의 능동소나 표적신호합성

신기철,박재은,김재수,최상문,김우식 한국해양대학교 해양과학기술연구소 2001 硏究論文集 Vol.10 No.1

능동소나 시스템에서 표적신호의 근거리 정보가 중요해짐에 따라, 표적 음향산란 신호의 고유한특성을 분석하기 위해 실험적인 방법과 시뮬레이션 기법이 사용된다. 본 논문에서는 표적신호 합성을 위해 축소표적 반향실험을 수행하였고, 자료의 분석 결과 축소표적의 음향산란은 거울면 반사의 영향이 큰 것으로 분석되어, 표적신호 합성에서 기하광학이론을 응용하였다. 복잡한 형태의 표적을 여러 표본형상으로 분리하고, 각 표본형상의 기여도를 신호의 위상과 강도를 줌으로써 계산하였으며. 표적신호 합성모델의 검증을 위해 실제 축소표적 실험 결과와 비교하였다. 혁심용어: 기하광학이론. 음향산란, 거울면반사. 축소표적, 능동소나 투고분야: 수중음향 분야 (5.2) Since the near field iformation of target signal is important in the development and verification of active sonar system experimental method and simulation technique are widely used in order to analyze the detail characteristics of target scattered echoes. Therefore, in this paper, the scale target experiment is performed to develope and improve the target signal simulation model. Since the experimental results show that the specular reflection is the major component among scattering mechanisms, the target signal simulation model based on the Geometric Optics Theory (GOT) is developed. Complex target is separated into simple shapes, known as canonical shape. The contribution from individual canonical shapes are summed with proper phase and amplitude to produse the target strength of the whole complex body. Simulated target signal is compared with the experimental results fed discussed.

Tufted Angioma와 유사한 소견을 보인 화농성 육아종

장경애,최정철,최지호,성경제,문기찬,고재경 대한피부과학회 1999 대한피부과학회지 Vol.37 No.6

Tufted angiomas are rare slowly progressive vascular lesions found typically in children and young adults, usually involving the trunk and neck. Microscopically, groups of capillary tufts, many uncanalized, were found dispersed at various levels in the dermis. Some authors regarded tufted angioma as a member of lobular capillary hemangiomas. We describe a pyogenic granuloma mimicking acquired tufted angioma on the lip in a 59-year-old woman. We suggest that a term lobular capillary hemangioma$quot; may be preferable in this case.

Epstein-Barr virus(EBV)와 연관된 Nasal type T/Natural Killer(NK)-cell lymphoma 1예

지두현,최지호,성경제,문기찬,고재경 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

Nasal type T/NK-cell lymphoma는 병리조직학적으로 응고성 괴사가 동반된 혈관중심성 침윤을 특징으로하며 T-cell과 NK-cell 항원을 모두 표현하는 면역조직화학염색 소견을 보이는 매우 드문 피부 림프종으로서 EBV와의 연관성이 흔히 보고되고있다. 저자들은 42세 여자에서 발생한, 전신적인 홍반성 구진, 판, 결절의 임상양상을 보였으며 피부 병변에서의 EBV-encoded RNA(EBER)에 대한 in situ hybridization상 양성소견을 보였고 전산화단층촬영, 골주사, 갈륨주사 등의 검사상 비강, 위장관 및 다른 내부장기 침범소견은 보이지 않아서 피부에만 국한된 경우로 사료되는 nasal type T/NK-cell lymphoma 1예를 보고하는 바이다.

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

n-CdS_(0.46)Se_(0.54)/p-Cu_92-x)S_(0.46)Se_(0.54) 이종접합 태양전지의 제작과 그 특성에 관한 연구

유상하,최승평,이상열,홍광준,서상석,김혜숙,전승룡,윤은희,문종대,신영진,정태수,신현길,김택성,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

승화방법에 의해 CdS_0.46Se_0.54 단결정을 성장하여 결정구조를 조사하고, Van der Pauw 방법으로 Hall effect를 측정하여 carrier density의 온도 의존성과 mobility의 온도 의존성을 조사하였다. 성장된 CdS_0.46Se_0.54 단결정을 치환반응하여 n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 이종접합 태양전지를 제작하였다. Spectral response, 전류-전압특성 및 전력변환 효율을 조사하여 그 결과로부터 개방전압은 0.48V, 단락 전류 밀도는 21mA/㎠, fill factor와 전력변환효율은 각각 0.75와 9.5%를 얻었다. CdS_0.46Se_0.54 single crystal was grown by a sublimation method. The crystal structure and the temperature dependence of carrier density and mobility of CdS_0.46Se_0.54 single crystal were studied. Heterojunction solar cells on n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 were fabricated by the substitution reaction. The spectral response, the J-U characteristics and the conversion efficiency of the n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells were studied. The open-cricuit voltage, short-circuit density, fill factor and conversion efficiency of n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells under 80mW/㎠ illumination were found to be 0.48V, 21mA/㎠, 0.75 and 9.5%, respectively.

Ether 및 Halothane마취시 정맥혈에서의 Lactate, Pyruvate 및 Excess lactate치의 변화에 대한 연구

김익수,최세진,백문기,황병두 대한마취과학회 1977 Korean Journal of Anesthesiology Vol.10 No.3

Venous blood lactate, pyruvate and excess lactate have been measured by the enzymatic method and calculated in 24 patients over a period of 2 hours during operation under general anesthesia. When the anesthetic was diethyl, ether, lactate and dyruvate levels showed a gradual increase significantly from 30 minutes and 60 minutes after induction of anesthesia respectively. Excess lactate appeared 30 minuts after induction of anesthesia, being increased slightly thereafter. When halothane was used, lactate level rose significantly in 120 minutes but no significant change in pyruvate and excess lactate was observed. In comparison to the results obtained in arterial blood during ether anesthesia by others, the levels of lactate and pyruvate found in this study were higher than those observed in arterial blood, whereas there was no considerable difference in change with duration of anesthesia and excess lactate. From the above results we suggested that venous blood lactate, pyruvate and excess lactate were essentially the same as those of arterial blood for evaluating tissue hypoxia during general anesthesia.

Electron Cyclotron Resonance O_2 Plasma에서 증착한 규소 산화 박막의 특성

안명환,서문석,장재선,서성모,이기방,윤창주,이형재,남기석,최규현,손춘배,김용섭,강석희 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

규소 산화막을 ECR-CVD(electron cyclotron resonance-chemical vapor deposition) 증착방법으로 5인치 기판위에 상온에서 증착하고, 증착공정조건인 증착율, 기판온도, 마이크로파의 세기변화 및 플라즈마 혼합기체의 비에 따른 규소 산화막의 특성을 조사하였다. 또한 산화막의 구조적인 특성을 비교하기 위해 FTIR을 이용하여 ECR-CVD 증착한 산화막, RPE-CVD(remote plasma enhanced-CVD) 증착한 산화막 및 열 산화막의 stretching frequency를 측정하였다. 측정된 결과 ECR-CVD로 증착된 산화막이 구조적인 면에서 열 산화막과 거의 같음을 보였다. ECR-CVD로 증착된 산화막의 전기적인 특성을 전류-전압 및 축전-전압 측정에의하여 분석하였다. 축전된 산화막의 전기적인 특성은 산화막의 전하 밀도는 1×10 exp (11)/㎠이였고, 평균 절연 파괴 전압은 약 6 MV/㎝이다. We have grown thin films of SiO_2 at room temperature by using an ECR-CVD system and have investigated the changes in the properties of the deposited films with changes in the processing conditions such as the deposition rate, the substrate temperature, the microwave power and the plasma gas mixing ratio. We also measured the stretching frequency of three kinds of oxides, and ECR-CVD-grown oxide, a PECVD-grown oxide, and a thermally grown oxide, using FTIR analysis to compare their structural properties. The result shows that the structural properties of the ECR-grown oxide are similar to those of the thermally grown oxide. Additionally, the electrical properties of the ECR-grown oxide were investigated by using current-voltage and capacitance-voltage measurements. These electrical results indicate that the oxide charge density and the average breakdown voltage are 1×10 exp (11) ㎝^-2 and 6 MV/㎝, respectively.

관동맥 재개통 후 QTc간격분산의 감소 : 정량적으로 측정한 심근 허혈의 호전 정도와의 연관성 Relationships with the Quantitative Degree of Improvement in Myocardial Ischemia

박상선,최기준,남기병,이철환,홍멸기,박성욱,박승정,신중우,문대혁,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

HIV 감염 환자에서 발생한 편평 콘딜롬 1예

이해웅,오상현,장성은,최지호,문기찬,고재경 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.4