Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

Hye-Min Kim,Ju-Sun Kim,Seon-Haeng Cho,Sam-Sik Kang,Dae-Sung Cheoi,Sang-Hyun Lee 한국약용작물학회 2006 한국약용작물학회지 Vol.14 No.5

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of H2O and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and 8.49 μg/mg, respectively), their fermented wine (0.45 and 1.33 μg/mg, respectively) and wine residues (no detection).

Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

Kim, Hye-Min,Kim, Ju-Sun,Cho, Seon-Haeng,Kang, Sam-Sik,Cheoi, Dae-Sung,Lee, Sang-Hyun The Korean Society of Medicinal Crop Science 2006 韓國藥用作物學會誌 Vol.14 No.5

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of $H_2O$ and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and $8.49\;{\mu}g/mg$, respectively), their fermented wine (0.45 and $1.33\;{\mu}g/mg$, respectively) and wine residues (no detection).

다양한 전처리 방법에 따른 양파의 이화학 및 영양학적 특성 분석

김광일 ( Kwang Il Kim ),황인국 ( In Guk Hwang ),유선미 ( Seon Mi Yu ),민상기 ( Sang Gi Min ),이상윤 ( Sang Yoon Lee ),최미정 ( Mi Jung Cho ) 한국산업식품공학회 2014 산업 식품공학 Vol.18 No.4

본 연구는 양파를 열처리 법 중에서 열수침지, 증기 및 볶음 처리에 따른 이화학적 및 영양학적 특성을 분석한 논문으로 열처리가 양파에 미치는 영향을 비교하고자 하였다. 상품을 구매할 때 1차적인 구매요소인 색도 및 경도측정과 추가적으로 pH와 조직관찰 결과로 최적조건 선정 후 영양학적 및 미생물 검사를 실시하였다. 열처리를 하지 않은 대조구에 비해 짧은 시간의 열처리에서는 더 진하고 선명한 색을 띠었으나, 장시간동안 처리한 열처리 시료는 색의 침착을 보였다. 원물과 처리구의 색은 열처리 시간에 따라 뚜렷한 차이를 나타냈고, 경도는 열처리 시간에 따라 짧은 시간에서는 분해효소의 불활성화로 유지 및 증가했지만, 3분 이상 처리하였을 때는 감소 경향을 나타냈고, 볶음처리는 짧은 시간으로도 물성이 연화됐다. 비타민은 열수 및 증기처리 1~2분 처리는 14% 정도 감소했지만 그 이상 처리 시에는 23~28% 범위로 큰 감소율을 보였으며, 유리당 및 유기산의 경우 열수침지 및 증기처리에서 손실을 보였지만, 볶음처리에서는 유지하거나 증가하는 경향을 나타냈다. 특히 유리당에서 열수침지 처리 시 높은 감소율을 나타냈다. Peroxidase activity 변화는 모든 처리구에서 75% 이상의 불활성화를 나타냈고, 특히 열수침지 및 증기 처리한 모든 시료에서 90% 이상의 불활성화를 나타내며 볶음처리보다 높은 불활성화율을 보였다. 미생물 측정한 결과 열수 침지 3분 이상 및 볶음처리 210초 이상 처리시 미생물 검출이 안됐으며, 증기처리 시료는 2분 처리만으로 사멸된 것을 관찰했다. 양파의 열처리 조건은 증기처리법으로 2분 동안 처리하는 것이 다른 처리법에서 처리한 시간들에 비해 최적으로 나타났다. 열처리 방법 별 각각의 최적 처리 시간을 가지고 있어, 높은 효율성이나 산업적으로 이용하려면 최적의 전처리 방법과 처리 시간을 산정하는 것이 중요하다. The pre-thermal treatment, or blanching, is an important process to minimize quality changes during main cooking or storage. In this study, three types of pre-thermal treatments were applied to onions to observe quality changes in their physicochemical, nutritional, and microbial properties. The washed and sliced onions were treated by hot-water immersion (100oC, 1~10 min), steaming (100oC, 1~10 min) and stir-frying with oil (180oC, 10~80 sec). The onions were cooled and wiped with gauze. The results showed that the total color difference of the treated sample increased to 18-23 units compared to that of the fresh control. The pH value of the onion was not significantly different with the exception of the stir-fried samples. The hardness of the onion tended to decrease depending on the treatment time. The micro-structures of onions treated by hot-water immersion and steaming were damaged above 2 min whereas stir-fried onions showed structural damage above 210 sec. In the analysis of the nutritional changes in the onion, the ascorbic acid content of the blanched treatments were lower than that of fresh onion and the free sugar contests showed a similar pattern excluding the stir-fried onions. In the organic acid content assay, the succinic acid content of the treated onion sample had significantly decreased compared with other organic acid contents (p<0.05). The peroxidase activities of the blanched treatments by hot-water immersion and steaming were inactivated by more than 92%, whereas only 76% inactivity was achieved by the stir-frying treatment. The mesophilic and psychrotrophic bacteria counts of the treated onion were not detected or decreased to near a 3-log scale, and yeast was detected only the sample treated by the stir-frying for 1 min. In particular, neither bacteria nor yeast counts in all onion samples by steam treatment were detected. Consequently, the results demonstrated that steam treatment for 2 min could be the optimal pre-thermal condition for onions to minimize the quality changes and increase safety.

노인에서 신체질환과 불면증 간의 전향적 연관성

김재민,김성완,양수진,김선영,배경열,조준영,윤탁,신일선,윤진상 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.5

Objectives : This study aimed to investigate the associations between physical disorders and prevalent/incident insomnia m a community dwelling older population. Methods : 1,204 people aged 65 or over evaluated at baseline. Insomnia was defined as difficulty in initiation or maintenance of sleep on at least three nights per week over the last month. Reported physical disorders covering 11 common and generally chronic health problems were ascertained. Covariates included were age, gender, education, housing, Past occupation, current employment, and depression. Of 879 without insomnia at baseline, 651 (74%) were followed 2 years later, and incident insomnia was evaluated. Results : Prevalent insomnia was significantly associated with 10 of 11 physical disorders. Incident insomnia was associated with 2 physical disorders : eyesight problems and hearing difficulty. Both prevalent and incident insomnia were significantly associated with increased number of physical disorders. Conclusion : Certain physical disorders were comorbid and precipitating factors of insomnia in elders.

初等學校 學生의 科學 探究能力과 學業 成就度간의 關係

박성민,조선형 淸州敎育大學校 科學敎育硏究所 1998 科學과 數學敎育論文集 Vol.19 No.-

The purpose of this study is to investigate the relationship between elementary school students' science inquiry skills and science achievements. The researcher used the science inquiry skill testing inventory developed by the study group in the Dept. of Physics, The Korea National University of Education, and science achievement test on a Unit changing season developed by the researcher. The result is as follow ; 1. On the basic science inquiry skills, the domains of observation, classifing, measuring, reasoning and expectation show higher scores than others domains, such as, analyzing matter, changing matters, assumption, controlling variable and generalization. 2. The mean score of science achievement test is 69.19. Students scored high in the knowledge domain, but low both of other domains, process and attitude. 3. On the science inquiry skills testing, male children scored 57.48 and female 65.85, especially, male children scored higher than female in classification by +4.96, but females showed higher scores than male children in the other 9 inquiry domains. 4. The correlation ratio between science inquiry skill and science achievement is r=+.72, showing high positive relationship. So, the science inquiry skill has favorable positive influence on the science achievement.

겨울철 홍천강의 위생학적 수질조사연구

오덕철,김선균,주왕기,조동현,김우호,윤경민 江原大學校附設體力硏究所 1976 江原大學校附設體育科學硏究所論文集 Vol.- No.1

The water quality of the Hong-Cheon river was investigated during the winter season. The rate of contamination of the low tide was a little more than the high tide. But all values of the analyzed-items were similar to those in the clear water.

농업 부산물 바이오매스 특성에 따른 반탄화 공정 최적화: 1. 질량감소 예측 모델 개발

김석준 ( Seok Jun Kim ),오광철 ( Kwang Cheol Oh ),박선용 ( Sun Young Park ),조라훈 ( La Hoon Cho ),김민준 ( Min Jun Kim ),이충건 ( Chung Geon Lee ),정인선 ( In Seon Jeong ),전영광 ( Yeong Kwang Jeon ),김대현 ( Dae Hyun Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.2

본 연구에서는 국내에서 버려지고 있는 농업부산물의 에너지로서 이용 가능성이 분석되었다. 농업부산물 바이오매스는 화석연료와 동일하게 활용가능하며 지속적으로 생산되는 에너지원이다. 하지만 높은 수분 함량에 따른 보관문제, 화석연료와 비교하여 낮은 발열량등의 단점을 지니고 있어 전처리공정이 요구된다. 따라서 효율적인 활용을 위한 전처리 공정으로 반탄화가 선정되었다. 반탄화공정은 수분제거를 통해 에너지가 증가되어 저장·운송에 이점으로 인하여 최종적인 에너지 가치가 증대된다. 실험은 문헌탐색을 통한 반탄화공정 적용 가능 농업부산물(고추대)과 실험조건(200, 230, 270C, 10, 20, 30, 40 min)이 선정되었으며, 1차원 시뮬레이션을 통한 분석이 이루어졌다. 이때 반탄화 실험을 통하여 발생되는 질량감소량과 발열량 변화량은 높은 상관관계(r²=0.9528)를 가지고 있으며 본 연구에서는 반탄화공정의 질량감소량 예측 모델개발이 수행되었다. 질량감소량 분석을 위하여 열 중량 분석(Thermo Gravimetric Analyzer, TGA)이 수행되었으며 승온속도(5, 7.5, 15, 22.5, 30, 37.5°C min^-1)에 따른 반응속도상수가 도출되었다. 1차원 시뮬레이션 분석을 통하여 바이오매스 내부 온도를 예측하였으며 앞서 도출된 반응속도 상수를 이용하여 질량감소량이 예측되었다. 질량감소량 실험 및 시뮬레이션 결과 평균 r²=0.97로 상관관계를 나타내고 있으며 공정온도별 평균제곱근오차(Root Mean Square Error, RMSE)가 200°C-0.0165, 230°C-0.0191, 270°C-0.0207로 나타났다. 공정온도 증가에 따라 정확도가 감소되었으며, 이는 고온의 실험조건에서 발생되는 비응축가스와 온도변화에 의한 열분해 특성에 기인한 것으로 판단된다. 최종적으로 더 나아가서 질량감소 모델을 통한 발열량 예측을 위한 분석이 이루어져야한다.

Two-dimensional gel electrophoresis and genetic analysis of S gene for self-compatibility in an interspecific buckwheat hybrid

Min-Hwa Park, Seong-Woo Cho, Tae-Seon Kim, Gi-Na Yun, Keun-Yook Chung, Seung-Keun Jong, Sun-Hee Woo 충북대학교 농업과학기술연구소 2007 農業科學硏究 Vol.23 No.2

-본문참고-

Thematic Poster : TP-84 ; IgG4-Related Lung Disease without Elevation of Serum IgG4 Concentration Level : Case Report

( Min Kyu Kang ),( Yong Seon Cho ),( Kyoung Min Moon ),( Min Soo Han ),( Sun Young Jung ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Since IgG4 related pancreatitis has been reported by Hamano et al. in 2001 for the first time, IgG4 related disease was seen from other organs such as salivary gland, gall bladder, thyroid, retroperitoneum and kidney but lung invasion was rare. A 63-year-old man visited department of pulmonary medicine complaining hemoptysis and about 4.1cm irregular shape mass with speculated margin was seen at left upper lobe in chest CT. Although there was no elevation of serum IgG4 concentration level, he finally diagnosed IgG4 related lung disease by TTNB. After the patient was treated with oral steroid, hemoptysis disappeared and lung mass size decreased.

Thematic Poster : TP-22 ; Tsutsugamushi Disease Combined with Necrosis of Toes

( Min Seok Kang ),( Yong Seon Cho ),( Kyoung Min Moon ),( Min Soo Han ),( Sun Young Jung ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Tsutsugamushi disease is an acute febrile disease which is characterized by fever, chilling, headache, skin rash, conjunctival injection, and eschar. In Korea, there has been an increasing concern over rickettsiosis since Tsutsugamushi disease among Koreans was first reported in 1986. A 72-year-old female patient was admitted to our hospital due to continous fever, chilling, headache which had been for ten days. Due to low blood pressure, norepinephrine was used for severeal days. The right 1st, 2nd, 3rd toe necrosis was started, then necrosis was completed, toes were amputated. in hospital day #1, we started antibiotics (meropenem, teicocin). R.tsutsugamusgi antibody was detected by hospital day #5, thus Doxycycline were administered to the patient for 29 days but the patient had consisited intermittent febrile condition. in hospital day #24, antibiotics had changed by ceftriaxon, azithromycin, fluconazole but fever had continued. in hospital day #72, no more fever was detected for 5days and the patient’s dietary intake was done well. so discharge was decided and then we would be examined the patient in the outpatient clinic.