아토피 피부염 모델에 대한 β-1,3/1,6-glucan과 Lactobacillus plantarum LM1004의 면역 조절 효과

김인성(In Sung Kim),김성학(Sung Hak Kim),김정아(Jeong A Kim),유다윤(Da Yoon Yu),김광일(Gwang Il Kim),박동찬(Dong-Chan Park),임종민(Jong Min Lim),이상석(Sang Suk Lee),최인순(In Soon Choi),조광근(Kwang Keun Cho) 한국생명과학회 2018 생명과학회지 Vol.28 No.1

본 연구에서는 아토피 피부염 동물 모델에 대한 β-1,3/1,6-glucan과 L. plantarum LM1004의 면역조절 효과를 확인하고자 하였다. 가려움증의 횟수와 유출된 evans blue, 그리고 혈청 IgE와 histamine의 농도는 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹에서 아토피 피부염 유발그룹에 비해 유의적으로 감소하는 결과를 나타내었다. 아토피 피부염이 유발되면 전사 수준에서 Th2 및 Th17 세포의 전사인자 및 cytokine은 과발현되며, β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취하였을 때 이를 유의적으로 감소되었다. 또한 β-1,3/1,6-glucan과 L. plantarum LM1004는 Th1 및 Treg 세포의 전사인자(T-bet, GATA-3, RORγT, Foxp3) 및 cytokine (INF-γ, IL-4, IL-17, TGF-β)의 발현을 증가시킴으로써 면역 균형을 조절하는 것으로 나타났다. Galectin-9과 filaggrin은 아토피 피부염 유발 처리군에서 유의적으로 가장 낮았으며, β-1,3/1,6-glucan 처리군에서 유의적으로 가장 높게 나타났다. 이와 반대로 TSLP는 아토피 피부염 유발그룹에서 유의적으로 가장 높았으며 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹은 대조군과 유사한 수준이었다. 이러한 결과를 통해 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염 동물 모델에서 면역조절 작용 및 아토피 피부염의 개선 효과를 가짐을 알 수 있었다. 따라서 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염에 유용한 천연소재로서 사용될 것으로 기대된다. In this study, we examined the efficacy of the immune regulation of β-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on atopic dermatitis models. The oral administration of β-1,3/1,6-glucan and L. plantarum LM1004 on mice significantly decreased the amount of scratching, leakage to evans blue, and concentrations of serum immunoglobulin E (IgE) and histamine compared with the atopic dermatitis–induced group. When atopic dermatitis was induced, the transcription factors (GATA-3, retinoic acid-related orphan receptor γ T [RORγT]) and cytokines (interleukin-4 [IL-4], IL-17) of Th2 and Th17 cells were overexpressed at the transcriptional level, and they significantly decreased with oral administration of β-1,3/1,6-glucan and L. plantarum LM1004. In addition, β-1,3/1,6-glucan and L. plantarum LM1004 were shown to modulate the immune balance by increasing the expression of Th1 and Treg transcription (T-bet, forkhead box p3 [Foxp3]) and cytokines (interferon-γ [IFN-γ], transforming growth factor-β [TGF-β]). Galectin-9 and filaggrin were significantly lower in the atopic dermatitis–induced group and significantly higher in the β-1,3/1,6-glucan-treated group. In contrast, thymic stromal lymphopoietin (TSLP) was highest in the atopic dermatitis–induced group, while mice that were orally administered β-1,3/1,6-glucan and L. plantarum LM1004 showed similar TSLP levels to the control group. These results indicate that β-1,3/1,6-glucan and L. plantarum LM1004 have immunomodulatory effects and atopic dermatitis improvement effects in an animal model of atopic dermatitis. Therefore, it is expected that β-1,3/1,6-glucan and L. plantarum LM1004 can be used as natural materials in the treatment of atopic dermatitis.

WLAN을 위한 5.2GHz/2.4GHz 이중대역 주차수 합성기의 설계

김광일,이상철,윤광섭,김석진,Kim, Kwang-Il,Lee, Sang-Cheol,Yoon, Kwang-Sub,Kim, Seok-Jin 한국통신학회 2007 韓國通信學會論文誌 Vol.32 No.1A

본 논문은 $0.18{\mu}m$ CMOS 공정으로 설계된 5.2GHz와 2.4GHz 이중 대역 무선 송수신기를 위한 주파수합성기를 제안한다. 2.4GHz 주파수는 스위치드 커패시터와 2분주기를 동작시켜서 발생시키고, 5.2GHz는 전압 제어 발진기의 출력 주파수로부터 직접 발생시키도록 설계하였다. 제안된 주파수합성기의 전체 전력소모는 25mW이며, 전압 제어 발진기의 전력소모는 3.6mW이다. 모의 실험된 주파수 합성기의 위상 잡음은 스위치드 커패시터 회로가 동작할 때, 200kHz 옵셋 주파수에서 -101.36dBc/Hz이고, 락킹 시간은 $4{\mu}s$이다. This paper presents a frequency synthesizer(FS) for 5.2GHz/2.4GHz dual band wireless applications which is designed in a standard $0.18{\mu}m$ CMOS1P6M process. The 2.4GHz frequency is obtained from the 5.2GHz output frequency of Voltage Controlled Oscillator (VCO) by using the Switched Capacitor (SC) and the divider-by-2. Power dissipations of the proposed FS and VCO are 25mW and 3.6mW, respectively. The tuning range of VCO is 700MHz and the locking time is $4{\mu}s$. The simulated phase noise of PLL is -101.36dBc/Hz at 200kHz offset frequency from 5.0GHz with SCA circuit on.

고혈압 환자에 대한 이베사탄의 혈압강하효과 및 안전성을 평가하기 위한 양측 눈가림, 무작위 배정, 에날라프릴과의 비열등성 4상 임상시험

김광일,배장환,강현재,서선예,김상현,오세일,채인호,조주희,김명아,김효수,손대원,오병희,박영배,최윤식,박병주,이명묵,Kim, Kwang-Il,Bae, Jang-Whan,Kang, Hyun-Jae,Suh, Sun-Ye,Kim, Sang-Hyun,Oh, Se-Il,Chae, In-Ho,Zo, Joo-Hee,Kim, Myoung-A,Kim, Hyo-Soo,Sohn 대한임상약리학회 2003 Translational and Clinical Pharmacology Vol.11 No.2

Background: Irbersartan, an orally active antihypertensive agent, effectively reduces blood pressure by blocking angiotensin II receptors without any significant adverse effects. The purpose of this study is to compare the antihypertensive efficacy, safety and tolerability of irbesartan and enalapril in hypertensive patients. Methods: In this two centers, double-blind, randomized, non-inferiority study, the efficacy, safety and tolerability of once-daily irbesartan(150mg) versus once-daily enalapril(10mg) were evaluated over 8 weeks in 67 patients who had diastolic pressure between 95mmHg and 114mmHg on two measurements. If trough sitting diastolic blood pressure was equal to or greater than 90mmHg after a 4-week treatment period, the dosage for both study drugs was doubled until the end of the study. Baseline pressures, antihypertensive effect, side effects, laboratory findings were compared before and after treatment. Results: Data from 57 of 67 patients were eligible for intention to treat analysis. After the 8 weeks treatment with dose titration, mean reductions in peak sitting diastolic blood pressure were 11.9mmHg(95% confidence interval 7.61 -16.13) with irbesartan and 10.9mmHg$(95%\;confidence\;interval\;7.05{\sim}14.70)$ with enalapril. There was no significant difference between the two treatments in the percentage of patients achieving an effective blood pressure reduction or in the degree of change in mean systolic and/or diastolic blood pressure. Safety profiles were also similar between treatments. Conclusions: The antihypertensive efficacy of once-daily administration of irbesartan is non-inferior to that of enalapril in hypertensive patients. Both irbesartan and enalapril are well tolerated with similar safety profiles.

RFID 태그 칩용 로직 공정 기반 256bit EEPROM IP 설계 및 측정

김광일,김려연,전황곤,김기종,이재형,김태훈,하판봉,김영희,Kim, Kwang-Il,Jin, Li-Yan,Jeon, Hwang-Gon,Kim, Ki-Jong,Lee, Jae-Hyung,Kim, Tae-Hoon,Ha, Pan-Bong,Kim, Young-Hee 한국정보통신학회 2010 한국정보통신학회논문지 Vol.14 No.8

본 논문에서는 logic 공정 기반의 소자만 사용한 256bit EEPROM IP를 설계하였다. 소자간의 전압을 신뢰성이 보장되는 5.5V 이내로 제한하기위해 EEPROM의 코어 회로인 CG (Control Gate)와 TG (Tunnel Gate) 구동 회로를 제안하였다. 그리고 DC-DC converter인 VPP (=+4.75V), VNN (-4.75V)과 VNNL (=VNN/3) generation 회로를 제안하였고 CG와 TG 구동 회로에 사용되는 switching power인 CG_HV, CG_LV, TG_HV, TG_LV, VNNL_CG와 VNNL_TG 스위칭 회로를 설계하였다. 일반적인 모의실험 조건에서 read, program, erase 모드의 전력 소모는 각각 $12.86{\mu}W$, $22.52{\mu}W$, $22.58{\mu}W$으로 저전력 소모를 갖는다. 그리고 테스트 칩을 측정한 결과 256bit이 정상적으로 동작을 하였으며, VPP, VNN, VNNL은 4.69V, -4.74V, -1.89V로 목표 전압 레벨이 나왔다. In this paper, we design a 256-bit EEPROM IP using only logic process-based devices. We propose EEPROM core circuits, a control gate (CG) and a tunnel gate (TG) driving circuit, to limit the voltages between the devices within 5.5V; and we propose DC-DC converters : VPP (=+4.75V), VNN (-4.75V), and VNNL (=VNN/3) generation circuit. In addition, we propose switching powers, CG_HV, CG_LV, TG_HV, TG_LV, VNNL_CG, VNNL_TG switching circuit, to be supplied for the CG and TG driving circuit. Simulation results under the typical simulation condition show that the power consumptions in the read, erase, and program mode are $12.86{\mu}W$, $22.52{\mu}W$, and $22.58{\mu}W$ respectively. Furthermore, the manufactured test chip operated normally and generated its target voltages of VPP, VNN, and VNNL as 4.69V, -4.74V, and -1.89V.

위암에서 E-cadherin과 $\beta-catenin$ 발현과 유전자 돌연변이에 관한 연구

김광일,박성혜,한선애,채양석,김인선,Kim Kwang Il,Park Sung-Hye,Han Sun-Ae,Chae Yang-Seok,Kim Insun 대한위암학회 2001 대한위암학회지 Vol.1 No.4

Purpose: When cancer cels invade the stroma, they should be dissociated from the adjacent cells at first. E-cadherin and $\beta-catenin$ constitute an important protein complex associated with cellular adhesion, development, and differentiation, especially in epithelial cells. The role of E-cadherin and $\beta-catenin$ in gastric carcinogenesis were studied. Materials and Methods: The expression of E-cadherin and $\beta-catenin$ in gastric adenocarcinomas by using immunohistochemical staining and the mutation by using polymerase chain reaction- single stranded conformation polymorphism (PCR-SSCP) and sequencing were performed in 40 adenocarcinomas and 5 dysplasia of stomach. Thirteen cases, which had lymph node metastasis, were also included for immunohistochemical staining. Results: Inappropriate cytoplasmic and/or nuclear expression of a E-cadherin-$\beta-catenin$ complex was more frequent in poorly differentiated, diffuse type signet ring cell carcinomas than in well-differentiated, intestinal type adenocarcinomas (P<0.05). However, the expression was not related with clinical stage or lymph node metastasis. Mutation of E-cadherin was detected in 4 cases by using PCR-SSCP, whereas mutation of $\beta-catenin$ was detected in 2 cases. Conclusion: E-cadherin and $\beta-catenin$ seem to be important in gastric carcinogenesis, especially in poorly differentiated diffuse type.

다발성의 분화도가 좋은 태아형 폐선암종 - 1예 보고 -

김광일,이주한,문정석,김한겸,Kim, Kwang-Il,Lee, Joo-Han,Mun, Jeong-Seok,Kim, Han-Kyeom 대한세포병리학회 1997 대한세포병리학회지 Vol.8 No.1

Well differentiated fetal adenocarcinoma of the lung Is a subtype of pulmonary blastoma. In this report, CT-guided fine needle aspiration smears were performed at the right upper lobe of the lung in a 45 year-old male patient who had the smoking history of one pack per day for 25 years. The smears disclosed round, papillary, and tubular patterns of cell clusters. The individual cells had relatively uniform, small to medium sized nuclei without nucleoli, and showed vesicular or eosinophilic cytoplasm with Indistinct cell border. The morules were seen in the central area of papillary clusters. They were composed of two cell types, outer single layered cuboidal cellular lining and central three-dimensional cluster of cells simulating fetal lung. These cytologic features need to be differentiated from usual pulmonary adenocarcinoma, carcinoid, and pulmonary blastoma. On histologic findings, the tumor arised in the bronchial epithelium. And the tumor cells had abundant intracytoplasmic glycogen with neuroendocrine feature on histochemical study. In addition, the multiplicity of this tumor is the unique point comparable to the previous reports.

프레임 분할과 3D 웨이블릿 변환을 이용한 비디오 워터마킹

김광일(Kwang-il Kim),최기철(Cui Jizhe),김종원(Jong-Weon Kim),최종욱(Jong-Uk Choi) 한국정보보호학회 2008 정보보호학회논문지 Vol.18 No.3

본 논문에서는 동영상 콘텐츠의 저작권 보호를 위해 3D 웨이블릿 변환과 대역확산방법(direct spread spectrum)을 이용한 비디오 워터마킹 알고리즘을 제안하였다. 제안한 방법은 동기 워터마크를 삽입한 다음 사용자 정보를 갖는 워터마크를 삽입하게 된다. 입력된 프레임을 분할하고, 다시 홀수행과 짝수행으로 정렬하여 각각 3D 웨이블릿 변환을 수행한 다음 대역확산 방법으로 워터마크를 삽입한다. 기존의 3D 웨이블릿을 이용한 비디오 워터마킹 알고리즘과는 달리 제안한 방법은 blind 방식으로써 원본 영상 없이 워터마크 검출이 가능하다. 실험결과 PSNR은 평균 40 dB 이상으로써 높은 비가시성을 만족하는 동시에 프레임 절삭, 잡음 첨가, 압축 등의 공격에서도 10% 이하의 BER을 기록하여 공격에 강인한 것으로 확인되었다. In this paper we proposed a video watermarking algorithm based on a three dimension discrete wavelet transform (3D DWT) and direct spread spectrum (DSS). In the proposed method, the information watermark is embedded into followed frames, after sync watermark is embedded into the first frame. Input frames are divided into sub frames which are located odd row and even row. The sub frames are arranged as 3D frames, and transformed into 3D wavelet domain. In this domain the watermark is embedded using DSS. Existing video watermarking using 3D DWT is non-blind method but, proposed algorithm uses blind method. The experimental results show that the proposed algorithm is robust against frame cropping, noise addition, compression, etc. acquiring BER of 10% or below and sustains level of 40dB or above on the average.

차선 및 차량 인식 성능 검증 용 데이터베이스 구축 방안

김광일(Kwang-il Kim) 한국자동차공학회 2011 한국자동차공학회 부문종합 학술대회 Vol.2011 No.5

This paper proposes a method of establishing database for lane detection and vehicle detection. In exiting methods, developers have been developed as a way to check one of the data. In the proposed method to be searched by subject and surrounding environment, because you can find information on the vulnerability of vision algorithms can be supplemented by intensive testing in the fragile environment, allowing to improve the performance of the algorithm. Forward collision warning (FCW) and High beam assist (HBA) after inserting the information table, if the information can be used to develop. The proposed method uses C++, My-SQL to establish database.

선박항로 위험도 평가 시뮬레이터 개발에 관한 연구

김광일(Kwang-Il Kim),정중식(Jung Sik Jeong),박계각(Gyei-Kark Park) 한국지능시스템학회 2013 한국지능시스템학회논문지 Vol.23 No.3

항구 등 선박교통이 밀집된 구역에서 선박의 사고는 빈번하게 발생한다. 해양사고는 한번 발생하면 대형사고로 이어질 확률이 높으므로, 철저한 해상교통관리가 필요하다. 본 연구에서는 실 해역의 통계적인 해상교통정보와 실시간 선박 통항분포를 바탕으로 항로 위험도를 정량적으로 평가하기 위한 시뮬레이터를 개발하고자 한다. 항로 위험도 평가 시뮬레이터는 항로정보 입력부, 통항분포 분석부, 알고리즘 실행부로 구성하였다. 또한 완도해역의 해상교통정보를 시뮬레이터에 적용하여 항로의 정량적인 항로 위험도를 산출하고자 한다. Accidents between ships occur frequently at the traffic congestion area. Once a maritime accidents occur, it is likely to end up with critical damaged accidents. This paper develop a simulator for assessing quantitative risk based on statical maritime traffic data and realtime traffic distribution. Ship"s passage risk assessment simulator consist of import of division of passage data, traffic distribution analysis and passage risk assessment analysis. Maritime traffic data of WANDO waterway apply to simulator for calculation of quantitative risk rate of waterway.

나노리포좀으로 코팅된 Hemicellulase가 당근의 연화작용에 미치는 효과

김광일 ( Kwang Il Kim ),조연지 ( Yeon Ji Jo ),김수진 ( Soojin Kim ),서재희 ( Jae Hee Seo ),민상기 ( Sang Gi Min ),조형용 ( Hyung Yong Cho ),신정규 ( Jung Kue Shin ),최미정 ( Mi Jung Choi ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.4

The objective is to investigate effect of liposome coated hemicellulose on softening of carrot. To encapsulate hemicellulose in nanoliposome, 2% hemicellulase and 2% lecithin were processed by using high-speed homogenizer (10,000 rpm, 3 min) and ultrasonification (200 W, 54%). The carrot were cut into cylinder type (3×1 cm) and then immersed in distilled water (DW, control), hemicellulase (He) and nanoliposome coated hemicellulose (He/NL) for 48 h at 4oC. The final concentration of hemicellulose is 1% (w/v). The droplets properties of He, NL, He/NL analyzed using zeta-sizer. Moreover, the carrots treated different immersed solutions were characterized by measuring hardness, color, microstructural observation and enzyme activity (glucose contents). For the results, hardness of carrot immersed in He or He/NL solution decreased after 48 h by 47% or 31% (outline of carrot) and 35% or 31% (center of carrot) respectively compared to control (7,240 g). The total color difference value of all samples increased over immersion time. For microstructural observation, cell was destroyed after 24 h at He solution. For the enzyme activity, glucose contents of carrot in He solution increased than it in He/NL at 0 h sample however the enzyme activity was no significant difference with immersion time.