Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

화병(火病)을 동반한 갱년기 환자 치험 5례

송유림 ( Yu-rim Song ),박경미 ( Kyung-mi Park ),양승정 ( Seung-jeong Yang ),이은규 ( Eun-kyu Lee ),김혜화 ( Hye-hwa Kim ),조성희 ( Seong-hee Cho ) 대한한방부인과학회 2016 大韓韓方婦人科學會誌 Vol.29 No.4

Objectives: The purpose of this study is to evaluate the effects of Korean traditional treatments for Climacteric Syndrome Patients with Hwa-Byung. Methods: From February 2015 to May 2015, We treated 5 Cases Patients with Korean traditional treatments for a month. We measured treatment effects by Menopause Rating Scale (MRS), Beck`s Depression Inventory (BDI), Visual Analogue Scale (VAS) and Digital Infrared Thermal Imaging (DITI). Results: After treatment, First, the scores of MRS, BDI were decreased. Second, the symptoms such as hot flush, pantalgia, palpitation, insomnia, fatigue were significantly improved. Also, the difference of ?中 (CV17, Danjung) and 關元 (CV4, Guanyuan) temperatures were significantly decreased after treatment. Conclusions: This study suggests that Korean traditional treatments such as Gamicheonglijagam-hwan, acupuncture, moxibustion are effective on Climacteric Syndrome Patients with Hwa-Byung.

청간소요산으로 호전된 설통 환자 3례

김연진,양승보,김정화,이상화,조승연,박성욱,박정미,고창남,Kim, Yeon-jin,Yang, Seung-bo,Kim, Jeong-hwa,Lee, Sang-hwa,Cho, Seung-yeon,Park, Seong-uk,Park, Jung-mi,Ko, Chang-nam 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.4

Objectives: This clinical study reports the effects of Cheonggansoyo-san (CSS) on three patients with glossodynia. Methods: Three patients with glossodynia were treated with CSS (i.e., an herbal medicine) and acupuncture. Numeric rating scales (NRSs) for glossodynia and dry mouth and hwa-byung questionnaire scores were evaluated, and the results from heart rate variability (HRV) evaluations were analyzed. Results: After treatment with CSS, three patients' glossodynia symptoms, such as burning sensations, tingling, and numbness of the tongue, were improved. NRS ratings for glossodynia and dry mouth and the hwa-byung questionnaire scores also improved, and HRV parameters were closer to the normal range. Conclusion: Cheonggansoyo-san could be used to treat glossodynia.

폐의 임파관 평활근종 -1예 보고-

지미경,양기화,김병기,김선무 대한병리학회 1984 Journal of Pathology and Translational Medicine Vol.18 No.4

일개 종합병원 입원환자의 낙상 실태 및 관련요인

양화미,천병철 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

Background: Toestimate fall incidence rate and associated factorsin inpatients from a generalhospital. Method: The data werecollected from 104 fall incident reportsdeveloped by the patient safety committee in a general hospital in Seoul from 01 January 2007 to 31 December 2008. Information included general characteristicsof patients, factors related to fall, types, places,circumstances and outcomes of fall. Result: The incidence rate of fall, which was 4.4 per 1,000 total dischargedpatientsand 0.5 per 1,000 patientdays, was much lower than that of several hospitals in the United States. The difference may reflect the different incidence reporting systemof each hospital. Fall-pronepatients were, in general, ~65 years of age, had an alert mental status, were ambulatory with some a ssistance, and were dependenton and ambulatory device. High incidence of falls was associated with patients with circulatory disease. The majority of fall event susually occurredin bed orat the bedsidein thepatient's room, and occurred more of tenduring the night than during the day or evening. Risk factors of fall wereuse of drugs (antihypertensive or neuropsychiatric drugs) and environmental factors (e.g., overly highbed height, surrounding objects, inadequate fitnessshoes and slippery floor). Physical injury occurred in 43.3% of fall events, which typically required diagnosis of injuryand treatmentsuchas suturing. Riskfactorsforrepeatedfallswereuseofa neuropsychiatric drug (oddsratio=13.9) and gait disturbance (oddsratio=91.2). Risk factors for fall-related injurywerealert mental status(oddsratio=3.3 timesmore likelyto fall than thosewhoweredrowsyor ina stupor) and general weakness(odds ratio=3.3 times more likelyto fall than thosewhowerenot generally weak). Conclusion : Medicaland nursing staff shouldbe awareof the fall risk factorsof hospitalized patientsand should intensively pursue preventative strategies. Development of fall prevention education based on these resultsis recommended.

Mycosis Fungoides 1예 보고

지미경,양기화,김병기,심상인,김선무 大韓血液學會 1984 大韓血液學會誌 Vol.19 No.1

만성정신질환자 가족의 부담감과 치료동반자역할의 필요성 및 참여도

양수,장미화 대한간호학회정신간호학회 2000 정신간호학회지 Vol.9 No.1

A better understanding of the therapeutic role and the burden on the family has become increasingly important because many families assume a major role in the care of their relatives who have mentally ill patients. Especially, care of psychiatric patients in Korea is family-centered. Therefore, the burden of family caregivers is severe, and families have strong affects in the treatment of psychiatric patients. However, due to various reasons, families lack understanding of patient status and diseases so they follow instructions of physicians passively and do not even think about participating in the treatment plans for patients. This study was designed to identify the quantities of burden perceived by the family caregivers, and to obtain basic data about need awareness and degree of participation of family members as treatment partners in the treatment of psychiatric patients. The data were collected from July 19 to September 27, 1999, and the subjects of the research were 106 family members of psychiatric patients who were either hospitalized or received outpatient treatment in 4 mental health centers and one psychiatric hospital located in Kyunggi Province and Seoul. The researchers developed and used a questionnaire as the evaluation tool to determine the awareness of need and degree of actual participation in family members. Also, subjective and objective burden measurement was used to measure burden of family caregivers. This tool originally developed by Montgomery(1985) and used by Choi(1997), and revised by researchers to 4-point Likert Scale. The results are as follows. 1. Although family was highly aware the need for the role of family members as treatment partners, the actual degree of family member participating was low. 2. As the degree of need awareness of family roles according to each area, families felt that they are needed most in the matters relating to admission and hospitalization of patients, followed by their roles at the time of discharge and daily life after discharge, but showed low awareness on roles relating to family association. 3. Participation of family members as treatment partners was seen most with matters related to discharge, and showed lowest participation rate in matters concerning family association. 4. The role awareness and participation degree of family members were significantly different according to the occupations of family members, and the degree of participation was significantly different according to marital status, forms of medical coverage, relationship with the patient, and opinions on patient prognosis. 5. The mean score of the perceived burden of the family caregivers was 2.64, which is higher than the mode score of instrument. 6. The burden of the family caregivers was significantly different according to gender, marital status, education level, occupation, relationship with the patient, and opinions on patient prognosis. 7. There was a significant correlation between the role awareness and participation degree as treatment partners of family members, and there was not a significant correlation between the burden and the participation degree. As seen with these results, by raising the awareness of family members as treatment partners, the study suggests that the area for family members to involve in more active patient treatment and care need to be expanded, and we also need systematic efforts such as to study ways tat relieve the burden of the family and make family members to participate in treatment and care of patients.

Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.

대구지역 거주자의 식생활 습관과 위장질환과의 관계

오미정,김수월,양희면,현재식,허천식,이명화 한국건강관리협회 2000 건협 Report Vol.3 No.-

유방초음파 검사의 유용성에 대한 연구

문양인,김병학,명화련,도윤수,김계환,손정훈,김미회 한국건강관리협회 2000 건협 Report Vol.3 No.-