Biosorption Behaviors of Biosorbents Based on Microorganisms Immobilized by Ca-alginate for Removing Lead (II) from Aqueous Solution

Xiaoli Li,Yaolong Wang,Yanfeng Li,Lincheng Zhou,Xiaoning Jia 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4

Multiple microorganisms directly or treated with NaOH were immobilized by using Ca-alginate embedding to form biosorbents I and II, successively. The biosorption behaviors of biosorbents I and II for Pb(II)from aqueous solution were investigated in a batch system. Effects of solution pH, initial metal concentration, biosorbent dosage, contact time, temperature, and ionic strength on the adsorption process were considered to study the biosorption equilibrium, kinetics, thermodynamics, and mechanism of Pb(II) ion adsorption on the 2 types of biosorbents. The results showed that the adsorption capacity of biosorbent II for Pb(II) was higher than that of biosorbent I, and biosorbent II had a faster adsorption rate for Pb(II) ions. According to FTIR spectra, the carboxyl,amine, and hydroxyl groups on the biomass surface were involved in the biosorption of Pb(II). EDX analysis showed that ion exchange may be involved in the biosorption process, and the morphology observed by SEM micrograph of biosorbent I was completely different from that of biosorbent II. Desorption and regeneration experiments showed that the 2 types of biosorbents could be reused for 3 biosorption-desorption cycles without significant loss of their initial biosorption capacities.

Identification of MicroRNAs as potential biomarkers for detecting ischemic stroke

Li Kexin,Shen Li,Zheng Pingping,Wang Yanjun,Wang Lijuan,Meng Xiaoli,Lv Yaogai,Xue Zhiqiang,Guo Xin,Zhang Anning,Pan Pan,Bi Chunli,Chen Yang,Feng Tianyu,Li Bo,Jin Lina,Yao Yan 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Increasing epidemic of ischemic stroke (IS) makes it urgent to understand the pathogenesis and regulatory mechanism, previous studies have described microRNAs (miRNAs) is part of the brain's response to ischemia. Objective: The aim of this study was to screen potential biomarkers for the prediction and novel treatment of IS. Methods: Differentially expressed miRNAs were screened from three newly diagnosed IS patients and three controls by RNA sequencing technology. Furthermore, target prediction databases were then used to analysis the target genes of different expressed miRNAs, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were used to identify the functions and the main biochemical and signal pathways of differentially expressed target genes. Results: Our results revealed that 27 miRNAs were differentially expressed in IS, among which, hsa-miR-659-5p was the most highly increased and was first found to be associated with IS. In addition, KEGG pathway analyses showed that differentially expressed miRNAs were mainly significantly enriched in lysosome pathway, cytokine-cytokine receptor interaction pathway, spliceosome pathway, base excision repair pathway. Conclusions: miRNAs were involved in IS pathogenesis, and hsa-miR-659-5p, hsa-miR-151a-3p and hsa-miR-29c-5p as the three highest |log2FoldChange| regulation in this study, which may be the biomarkers of IS and need further study.

Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii

Li Chaoqiong,Hu Lizong,Que Beibei,Hu Yueran,Guo Yuanyuan,Zhang Minghui,Wang Zenan,Wang Xueqin,Liu Hongzhan,Wang Junsheng,Tian Huihui,Li Xiaoli 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background Paeonia ostii seeds were identifed as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefts. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds. Objectives This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Methods Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR. Results The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and fve gene groups with distinct temporal patterns during seed development were identifed by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed. Conclusions This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.

PM2.5 Estimation Based on Image Analysis

( Xiaoli Li ),( Shan Zhang ),( Kang Wang ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.2

For the severe haze situation in the Beijing-Tianjin-Hebei region, conventional fine particulate matter (PM2.5) concentration prediction methods based on pollutant data face problems such as incomplete data, which may lead to poor prediction performance. Therefore, this paper proposes a method of predicting the PM2.5 concentration based on image analysis technology that combines image data, which can reflect the original weather conditions, with currently popular machine learning methods. First, based on local parameter estimation, autoregressive (AR) model analysis and local estimation of the increase in image blur, we extract features from the weather images using an approach inspired by free energy and a no-reference robust metric model. Next, we compare the coefficient energy and contrast difference of each pixel in the AR model and then use the percentages to calculate the image sharpness to derive the overall mass fraction. Furthermore, the results are compared. The relationship between residual value and PM2.5 concentration is fitted by generalized Gauss distribution (GGD) model. Finally, nonlinear mapping is performed via the wavelet neural network (WNN) method to obtain the PM2.5 concentration. Experimental results obtained on real data show that the proposed method offers an improved prediction accuracy and lower root mean square error (RMSE).

Adaptive NN Tracking Control for Periodically Time-varying Nonlinear Switching Systems

Xiaoli Yang,Jing Li,Jian Wu,Xiaobo Li 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12

This paper tackles the tracking control problem for a class of uncertain switching nonlinear systems with periodically time-varying parameters. To handle the unknown nonlinear functions and periodically time-varying parameters simultaneously, the radial basis function neural networks and Fourier series expansion are combined together. At the same time, the command filter approach is utilized to eliminate the “explosion of complexity” problem of the traditional backstepping technique and relax the assumption on the reference signal as well. Compared with the previous results, the filter errors are compensated by introducing the compensated signals and the upper bounds of the approximation errors are estimated as well. A compound adaptive neural network control strategy is proposed to warrant that the closed-loop system is semi-globally ultimately uniformly bounded. Two examples are provided to demonstrate the effectiveness of the reported approach.

예측 배송 시스템을 위한 수요 예측 모델링

이소려(LI XIAOLI),이동우(DongWoo Lee) 한국정보기술학회 2016 Proceedings of KIIT Conference Vol.2016 No.6

125I Seed Permanent Implantation as a Palliative Treatment for Stage III and IV Hypopharyngeal Carcinoma

Lei Li,Jie Yang,Xiaojiang Li,Xiaoli Wang,Yanxin Ren,Jimin Fei,Yan Xi,Ruimei Sun,Jing Ma 대한이비인후과학회 2016 Clinical and Experimental Otorhinolaryngology Vol.9 No.3

Objectives. The aim of this study was to investigate the feasibility and safety of percutaneous 125I seed permanent implantation for advanced hypopharyngeal carcinoma from toxicity, tumor response, and short-term outcome. Methods. 125I seeds implant procedures were performed under computed tomography for 34 patients with advanced hypopharyngeal carcinoma. We observed the local control rate, overall survival, and acute or late toxicity rate. Results. In the 34 patients (stage III, n=6; stage IV, n=28), the sites of origin were pyriform sinus (n=29) and postcricoid area (n=5). All patients also received one to four cycles of chemotherapy after seed implantation. The post-plan showed that the actuarial D90 of 125I seeds ranged from 90 to 158 Gy (median, 127 Gy). The mean follow-up was 12.3 months (range, 3.4 to 43.2 months). The local control was 2.1–31.0 months with a median of 17.7 months (95% confidence interval [CI], 13.4 to 22.0 months). The 1-, 2-, and 3-year local controls were 65.3%, 28.6%, and 9.5% respectively. Twelve patients (35%) died of local recurrence, fourteen patients (41%) died of distant metastases, and three patients (9%) died of recurrence and metastases at the same time. Five patients (15%) still survived to follow-up. At the time of analysis, the median survival time was 12.5 months (95% CI, 9.5 to 15.4 months). The 1-, 2-, and 3-year overall survival rates were 55.2%, 20.3%, and 10.9%, respectively. Five patients (15%) experienced grade 3 toxic events and nine patients (26%) have experienced grade 2 toxic events. Conclusion. This review shows relatively low toxicity for interstitial 125I seed implantation in the patients with advanced stage hypopharyngeal cancer. The high local control results suggest that 125I seed brachytherapy implant as a salvage or palliative treatment for advanced hypopharyngeal carcinoma merit further investigation.

IRE1α protects against osteoarthritis by regulating progranulin-dependent XBP1 splicing and collagen homeostasis

Liang Li,Zhang Fengmei,Feng Naibo,Kuang Biao,Fan Mengtian,Chen Cheng,Pan Yiming,Zhou Pengfei,Geng Nana,Li Xingyue,Xian Menglin,Deng Lin,Li Xiaoli,Kuang Liang,Luo Fengtao,Tan Qiaoyan,Xie Yangli,Guo Fen 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Osteoarthritis (OA) is a full-joint, multifactorial, degenerative and inflammatory disease that seriously affects the quality of life of patients due to its disabling and pain-causing properties. ER stress has been reported to be closely related to the progression of OA. The inositol-requiring enzyme 1α/X-box-binding protein-1 spliced (IRE1α/XBP1s) pathway, which is highly expressed in the chondrocytes of OA patients, promotes the degradation and refolding of abnormal proteins during ER stress and maintains the stability of the ER environment of chondrocytes, but its function and the underlying mechanisms of how it contributes to the progression of OA remain unclear. This study investigates the role of IRE1α/ERN1 in OA. Specific deficiency of ERN1 in chondrocytes spontaneously resulted in OA-like cartilage destruction and accelerated OA progression in a surgically induced arthritis model. Local delivery of AdERN1 relieved degradation of the cartilage matrix and prevented OA development in an ACLT-mediated model. Mechanistically, progranulin (PGRN), an intracellular chaperone, binds to IRE1α, promoting its phosphorylation and splicing of XBP1u to generate XBP1s. XBP1s protects articular cartilage through TNF-α/ERK1/2 signaling and further maintains collagen homeostasis by regulating type II collagen expression. The chondroprotective effect of IRE1α/ERN1 is dependent on PGRN and XBP1s splicing. ERN1 deficiency accelerated cartilage degeneration in OA by reducing PGRN expression and XBP1s splicing, subsequently decreasing collagen II expression and triggering collagen structural abnormalities and an imbalance in collagen homeostasis. This study provides new insights into OA pathogenesis and the UPR and suggests that IRE1α/ERN1 may serve as a potential target for the treatment of joint degenerative diseases, including OA.

The Burholderia pyrrocinia Purple Acid Phosphatase Pap9 Mediates Phosphate Acquisition in Plants

Xiaoli Zhu,이승엽,양원태,이선우,백동원,Ming Shun Li,김도훈 한국식물학회 2019 Journal of Plant Biology Vol.62 No.5

Increasing the expression level of purple acidphosphatases (PAPs), which hydrolyze organic phosphate toinorganic phosphate (Pi), is an important response to Pi starvationin plants. PAPs are widely distributed in eukaryotes likemammals and plants but distributed in limited microorganisms. A PAP-like protein named Pap9 from Burkholderia pyrrociniaCH-67 was isolated. The enzyme had optimal activity atatypical pH of 8.5 and temperature of 85°C. When pap9 wasoverexpressed in Arabidopsis thaliana, the APase activity inthe transgenic plants was about 15% to 40% higher underacidic conditions and had an approximately four-fold increaseunder alkaline conditions compared with wild type. Overexpression of pap9 in plants enhanced APase activityunder Pi-deficient conditions more than under Pi-sufficientconditions. In-gel assays revealed that Pap9 exists as amonomer in transgenic plants. Root surface-associatedAPase activity in transgenic plants increased dramaticallyduring acidic and Pi-deficient conditions. These resultsindicated that bacterial Pap9 in transgenic plants wassecreted onto the root surface and released into the rhizosphere. The transgenic plants have significantly enhanced biomassthat have the potential for use in biotechnologicalapplications.

Finite element simulation and experiments on a new induction heating bearing replacer

Li Guofa,Ren Xiaoli,Wang Longshan,Zhang Donglin 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.8

A new shaft-bearing replacer used for bearing inner race based on the induction-heating technique by finite element method (FEM)was designed in this paper. The regularities of the distribution of the electromagnetic and thermal-structural coupled field in the replacer,temperature changes with time course, and expansion size in shaft and the bearing inner ring were simulated during the course of induction heating. The FEM simulation results not only proved the existence of the eddy skin effect, but also achieved the design parameters of the replacer. Experiments verified that the replacer can realize a fast disassembling of the bearing inner race, which assembled on an axle with interference fits.