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- 저자 : Wang Zenan (검색결과 3 건)
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Li Chaoqiong,Hu Lizong,Que Beibei,Hu Yueran,Guo Yuanyuan,Zhang Minghui,Wang Zenan,Wang Xueqin,Liu Hongzhan,Wang Junsheng,Tian Huihui,Li Xiaoli 한국유전학회 2021 Genes & Genomics Vol.43 No.8
Background Paeonia ostii seeds were identifed as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefts. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds. Objectives This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Methods Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR. Results The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and fve gene groups with distinct temporal patterns during seed development were identifed by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed. Conclusions This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.
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The Application of Decellularized Adipose Tissue Promotes Wound Healing
Xia Zenan,Guo Xiao,Yu Nanze,Zeng Ang,Si Loubin,Long Fei,Zhang Wenchao,Wang Xiaojun,Zhu Lin,Liu Zhifei 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6
Background: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized. Methods: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies. Results The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2–24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect. Conclusion: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.
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Xinyue Zhang,Bushi Dai,Shucheng Ren,Zenan Hu,Xin Zheng,Yao Wang,Hongbin Sun,Dun Niu,Linshan Wang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5
Due to the complexity of the contaminations in wastewater, a universal adsorbent that takes many sorts of pollutant is desired. A multi-functioning magnetic Fe3O4-cored magnesium-aluminum layered double oxide (Fe3O4@LDO) composite was prepared and employed as an adsorbent for removing F-, Sb(III) and MO from water, respectively, which are three typical contaminants with different molecule sizes as water contamination. The effect of pH contact time and initial concentrations of pollutants on the removal rates of each contaminant were investigated. Fe3O4@LDO composite showed excellent adsorption performance for all of the three molecules F-, Sb(III) and MO, and their maximum adsorption capacities are as high as 258, 391, and 506mg/g, respectively. The adsorption process of F-, Sb(III) and MO on Fe3O4@LDO fitted well to Langmuir model and pseudo-second-order kinetic model. The adsorption mechanisms of F-, Sb(III) and MO on Fe3O4@LDO were investigated. We discovered that iron ions diffused from Fe3O4 nanospheres contributed to the good performance of the absorbent.
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