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- 저자 : Tian Huihui (검색결과 4 건)
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Anti-collision Device for the One-Dimensional Scanning Probe Fitted to a Measuring Machine
Huihui Tian,Hongxi Wang,Guanwei Wang,Wenhong Liang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.11
The probe is at the heart of the measuring machine operation. A variety of probe types and designs are available today. However, many users continue to use the conventional one-dimensional scanning ones because of their low cost, adequate level of accuracy and performance. During the measurement process, impact phenomena between the probe ball and the part surface occur frequently due to inaccurate programming of the software and improper measurement operation, as well as the one-dimensional scanning probe is infinitely stiff in the motion direction along the machine axis, resulting in plastic deformation of the probe stylus and damage of the probe sensor. It is therefore desirable to protect the scanning probe against collision with a workpiece which is to be measured. A new anti-collision device incorporating a compliant mechanism and permanent magnets is designed in this paper. The analysis of dynamic performance for anti-collision system regarding contact force, magnetic force, velocity, position, has been performed and the maximum contact force in the event of collision is available. Numerical results and prototype experiments show the validity of the designed anti-collision device for the one-dimensional scanning probe.
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PEG-mediated transduction of rAAV as a platform for spatially confined and efficient gene delivery
Liang Zou,Jinfen Wang,Ying Fang,Huihui Tian 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4
Background: Recombinant adeno-associated viruses (rAAV) are commonly used vectors for gene delivery in both basic neuroscience and clinical applications due to their nonpathogenic, minimally immunogenic, and sustained expression properties. However, several challenges remain for the wide-scale rAAV applications, including poor infection of many clinically important cell lines, insufficient expression at low titers, and diffusive transduction in vivo. Methods: In this work, PEG, which is a safe and non-toxic polymer of ethylene oxide monomer, was applied as an auxiliary transduction agent to improve the expression of rAAV. In detail, a small dose of PEG was added into the rAAV solution for the transgene expression in cell lines in vitro, and in the central nervous system (CNS) in vivo. The biocompatibility of PEG enhancer was assessed by characterizing the immune responses, cell morphology, cell tropism of rAAV, neuronal apoptosis, as well as motor function of animals. Results: The results show that small dose of PEG additive can effectively improve the gene expression characteristics of rAAV both in vitro and in vivo. Specifically, the PEG additive allows efficient transgene expression in cell lines that are difficult to be transfected with rAAV alone. In vivo studies show that the PEG additive can promote a spatially confined and efficient transgene expression of low-titer rAAV in the brain over long terms. In addition, no obvious side effects of PEG were observed on CNS in the biocompatibility studies. Conclusions: This spatially confined and efficient transduction method can facilitate the applications of rAAV in fundamental research, especially in the precise dissection of neural circuits, and also improve the capabilities of rAAV in the treatment of neurological diseases which originate from the disorders of small nuclei in the brain.
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Polymer nanofiber network reinforced gold electrode array for neural activity recording
Siting Yang,Ke Xu,Shouliang Guan,Liang Zou,Lei Gao,Jinfen Wang,Huihui Tian,Hui Li,Ying Fang,Hongbian Li 대한의용생체공학회 2023 Biomedical Engineering Letters (BMEL) Vol.13 No.2
Flexible and stretchable neural electrodes are promising tools for high-fidelity interfacing with soft and curvilinear brainsurface. Here, we describe a flexible and stretchable neural electrode array that consists of polyacrylonitrile (PAN) nanofibernetwork reinforced gold (Au) film electrodes. Under stretching, the interweaving PAN nanofibers effectively terminate theformation of propagating cracks in the Au films and thus enable the formation of a dynamically stable electrode-tissue interface. Moreover, the PAN nanofibers increase the surface roughness and active surface areas of the Au electrodes, leading toreduced electrochemical impedance and improved signal-to-noise ratio. As a result, PAN nanofiber network reinforced Auelectrode arrays can allow for reliable in vivo multichannel recording of epileptiform activities in rats.
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Li Chaoqiong,Hu Lizong,Que Beibei,Hu Yueran,Guo Yuanyuan,Zhang Minghui,Wang Zenan,Wang Xueqin,Liu Hongzhan,Wang Junsheng,Tian Huihui,Li Xiaoli 한국유전학회 2021 Genes & Genomics Vol.43 No.8
Background Paeonia ostii seeds were identifed as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefts. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds. Objectives This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Methods Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR. Results The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and fve gene groups with distinct temporal patterns during seed development were identifed by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed. Conclusions This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.
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