Acetate와 Glucose가 혼합되어 있는 배지에서 성장하는 당근 현탁배양 세포의 diauxic growth

李宅堅,李宇成 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.2

우리는 이제까지 bacteria와 yeast 등에서 관찰되어 온 diauxic growth 현상이 식물에서도 관찰되는지에 대해 연구해왔다. 우리는 이미 단자엽(벼) 현탁배양을 acetate와 glucose 가 혼합되어 있는 배지에서 성장시켰을 때 diauxic growth 현상이 있음을 보고하였다 (1). 우리는 쌍자엽식물에서도 diauxic growth가 관찰되는지 알아보기 위하여 당근의 현탁배양을 이용하여 연구하였다. 당근의 현탁배양세포를 glucose와 acetate가 혼합되어 들어있는 배지에서 성장시켰을 때 diauxic growth가 관찰되었다. Diauxic growth의 첫 번째 성장기에 acetate를 사용하고 두 번째 성장기에 glucose를 사용한다. 첫 번째 성장기에 있는 세포는 [^14C]acetate를 흡수하지만 [^14C]glucose 는 흡수하지 못했다. [^14C]glucose 흡수는 두 번째 성장기 세포에서 관찰할 수 있었다. 벼의 현탁배양 세포와 마찬가지로 당근의 현탁배양세포도 acetate를 일차적인 탄소원으로 사용하는 diauxic growth가 관찰되었다. We have been investigating acetate-glucose diauxic growth in plant. It was reported that rice (monocotyledon) suspension cells were exhibited diauxic growth on acetate (10 mM) and glucose (10 mM) medium (1). To investigate diauxic growth in dicotyledoneous plant, carrot (Daucus carota L.) suspension cells were grown on acetate and glucose medium Suspension culture of carrot exhibited diauxic growth when grown in a medium containing both acetate and glucose as carbon sources. Preferential utilization of acetate was observed in the initial growth phase of diauxic growth, whereas the second growth phase occured at the expense of glucose. Cells harvested during the first growth phase exhibited a capacity uptake of [^14C]acetate but not of [^14C]glucose. A capacity for [^14C]glucose uptake was expressed during the second growth phase. Like in rice suspension cells, in carrot suspension cells acetate was used as the primary carbon source during the acetate-glucose diauxic growth.

조직학적으로 진단된 담낭용종의 임상적 특징

이석호,최윤호,손희정,류광현,이규택,김재준,김용일,최규완,최성호,백승운,이종균,고광철,이풍렬,이종철,노재형,이준혁,심상군 대한소화기학회 1999 대한소화기학회지 Vol.34 No.1

Background/Aims: We aimed to clarify clinical factors which suggest the possibility of neoplastic polyps of gallbladder. Methods: Ninety-five resected gallbladders with polyps were included. Size and number of the polyps were obtained by preoperative ultrasonography. Histollogically, the polyps were classified into non-neoplastic (cholesterol and inflammatory or hyperplastic polyp) and neoplastic lesions (adenoma and adenocarcinoma). Clinical features, maximum diameter and number of the polyps were compared between the two groups. Results: Of 95 patients, non-neoplastic polyps were observed in 65.3% and neoplastic polyps were observed in 34.7%. We found cholesterol polyp in 47.4%, inflammatory or hyperplastic polyp in 17.9%, adenoma in 25.2% and adenocarcinoma in 9.5%. Two groups showed no difference in age, sex, body mass index, cholesterol, triglyceride, glucose, ALT, HBsAg carriage and the number of polyps. Among the patients with non-neoplastic polyps, 70.2% was under 60 years, while 72.7% of the patients with neoplastic polyps was over 60 years (p$lt;0.05). The size of polyps was under 10 mm in 88.9% of non-neoplastic polyps, and between 10 and 15 mm in 40% of neoplastic leisions. Moreover, 68.4% of neoplastic polyps exceeds 15 mm in diameter (p$lt;0.05). Conclusions: The size of polyp (≥10 mm) and patient's age (≥60 years) could be indicators for neoplastic polyps.

십이지장 제2부에 전이된 자궁경부암 1예

이화영,손희정,이규택,김재준,백승운,이종균,이준혁,고광철,이풍렬,이종철,손정일,서연림,전성국 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.1

In Korea, careinoma of the cervix is the most common malignancy in women annually affecting 25 per every 100,000 female. The widespread use of the Papanicolaou smear has resulted in earlier detection of the tumor and a reduction in the number of patients with advanced disease, but the incidence of cervix. carcinoma is still the fifth most common malignancy in the world. Cervical carcinoma spreads to the small bowel primarily by direct extension from involved lymph nodes, most commonly from peraaortic or mesenteric nodes to the serosa of the bowel. But, the involvement of small bowel by cervical carcinoma is so rare, and shows a maximum incidence of small bowel involvement of less than 1.5%. We report a patient with squamous cell carcinoma of the cervix metastases to the second portion of the duodenum in whom the diagnosis was made by endoscopic biopsy of the lesion.

복강경으로 진단된 복막 위점액종 1예

이종철,이석호,이규택,김재준,이종균,고광철,이풍렬,최규완,이준혁,오영륜,박근칠 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.3

Pseudomyxoma peritonei is a benign mucin producing tumor of the peritoneum which is usually diagnosed using a laparotomy. It is uncommon to find a case of pseudomyxoma peritonei which has been diagnosed using a peritoneoscopy in Korea; there are only two cases reported in the literature. We recently experienced a case of pseudomyxoma peritonei in a 61 year old woman who manifested a typical case using a peritoneoscopy. Thick, jelly-like materials were adherent to polypoid nodular masses of the parietal peritoneum, which originated from the mucinous cystadenocarcinoma of an ovary. In this report we discuss the case with relevant review of the literature.

내시경적 조직검사로 진단된 위 분선충증 1 예

이석호,이화영,이규택,강인구,최규완,백승운,이종균,이준혁,고광철,이종철,오영륜,현재근,이풍렬,김재준,채종일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis which exists in two forms : the free living and parasitic forms. It exists in warm, moist climate in areas where there is frequent fecal contamination of the soil. After cutaneous invasion by the filariform larvae, petechial hemorrage, pruritus, papular rashes, edema, and urticaria occur. Infection commonly occurs in the proximal intestine of the gastrointestinal (G-I) tract but may extend from the stomach to the anus. Once the worm is established in the small intestine, the physical findings may include epigastric tenderness to palpation. The mucosal biopsy is an inefficient way of making the diagnosis because the worm is found in the biopsy specimen in only 2% of patients. Gastric strongyloidiasis is rare. We experienced a case of gastric strongyloidiasis diagnosed by the endoscopic biopsy and serologic test for parasite specific IgG antibody by micro-ELISA.

한국인에서 네 번째 발병된 장카필라리아증 1예

이석호,이상구,류광현,고광철,이규택,김재준,최규완,이남용,백승운,이종균,이준혁,이종철,심상군,이풍렬,조승렬 대한소화기학회 1999 대한소화기학회지 Vol.34 No.4

Intestinal capillariasis is caused by Capillaria philippinensis. Among them, only 4 species have been found in humans: Capillaria hepatica, C. aerophila (Eucoleus aerophilus), C. plica and C. philippinensis. Reports of human infections with C. hepatica, C. aerophila and C. plica are rare, but reports of C. philippinensis infections are increasing and spreading geographically. Human infestation by C. philippinensis is a rare parasitic disease. We experienced a case of intestinal capillariasis which was misdiagnosed for intestinal tuberculosis or Crohn's disease at first but correctly diagnosed later by detection of numerous eggs of C. philippinensis on the stool examination. The eggs were found in stool only after ingestion of prednisolone for treatment of misdiagnosed Crohn's disease. The 42 year-old patient had a journey to Saipan island in Pacific ocean 6 months ago and ate sliced raw eels. Abdominal pain, watery diarrhea were subsided after therapy of albendazole.

간암 환자의 술전 검사로서 CTAP와 CTHA 의 유용성

이석호,이규택,김재준,강인구,김승훈,백승운,도영수,이종균,이준혁,고광철,이풍렬,최규완,오성윤,이종철 대한간학회 1997 Clinical and Molecular Hepatology(대한간학회지) Vol.3 No.4

Background / Aims : Combined computed tomography during arterial portography(CTAP) and computed tomography during hepatic arteriography(CTHA) are more sensitive methods compared to conventional spiral CT in detection small hepatocellular carcinoma. However, there are few data available on the influence of combined CTAP and CTHA on decision making for treatment of hepatocellular carcinoma. We aimed to evaluate the usefulness of CTAP and CTHA as a preoperative study of hepatocellular carcinoma for surgical treatment. Methods : Eighty -two patients with histologically proven hepatocellular carcinoma who were considered to be surgical candidates on spiral CT and angigraphy were included. CTAP and CTHA were performed as the routine protocol. The findings of CTAP and CTHA were analyzed and compared to conventional spiral CT and angiography. Results : 1)105 nodules were found on spiral CT plus angiography , whereas, 138 nodules found on combined CTAP and CTHA : 33 additional nodules were found in 23 cases(28%). 2) The treatment plan changed from surgery to nonsurgical treatment in 10(12%) cases : 9 cases(10%) with bilobar involvement, and 1 case(1%) with more than three nodules. 3) The larger the tumor nodules on the spiral CT, the more additional tumor nodules were found on CTAP and CTHA : combined CTAP and CTHA were able to find more nodules in 21% of tumor ≤3cm, 27% of tumor with 3- 10cm, and 71% of tumor > 10 am on spiral CT (p=0.03). Conclusion : Compared to spiral CT plus angiography, CTAP and CTHA may be useful in avoiding unnecessary operation of hepatocellular carcinoma, especially in large tumors.

Therapeutic Strategy for the Prevention of Pseudorabies Virus Infection in C57BL/6 Mice by 3D8 scFv with Intrinsic Nuclease Activity

Lee, Gunsup,Cho, SeungChan,Hoang, Phuong Mai,Kim, Dongjun,Lee, Yongjun,Kil, Eui-Joon,Byun, Sung-June,Lee, Taek-Kyun,Kim, Dae-Hyun,Kim, Sunghan,Lee, Sukchan Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.9

3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and $10{\mu}g$ 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 $LD_{50}$ PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% ($5{\mu}g$) and 47% ($10{\mu}g$). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.

한국인에서 발생한 섬유층판 간세포암 1예

이석호,조재원,이규택,강인구,최규완,백승운,이종균,고광철,이종철,박철근,이준혁,오영륜 대한소화기학회 1998 대한소화기학회지 Vol.32 No.1

Fibrolamellar hepatocellular carcinoma (FLHCC) is a distinct variant of ordinary hepatocellular carcinoma (HCC) and is very rare in the Orient. Contrary to ordinary HCC, the FLHCC has very good prognosis and usually occurs in adolescents and young adults without sexual preference. In addition, it is rarely associated with liver cirrhosis and hepatitis B virus. The FLHCC presents a high resectability rate. Most cases of FLHCC have been reported in the Western countries and only nine cases have been reported in the Orient. We present here a case of FLHCC observed in a 48 year old man. The patient had no subjective symptoms when the tumor was revealed by ultrasonography. The patient had many ch'arateristics of FLHCC (i.e,, no association with HBV or HCV, normal range of alphafetoprotein, no evidence of cirrhosis). However, he was older than average-aged patient with FLHCC. The tumor was removed by right antero-inferior segmententomy of the liver and diagnosed as FLHCC. To our knowledge, this report is the fast case in Korea.

Synthesis of Pt on Surface of Metallic Nano-particles using a Combination of Electrical Wire Explosion and Polyol Processes

Taek-Kyun Jung,Dong-Woo Joh,Hyo-Soo Lee,Seung-Yub Lee 한국표면공학회 2013 한국표면공학회 학술발표회 초록집 Vol.2013 No.11