난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화

이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3

Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.

VRSA에 대한 항생물질을 생산하는 방선균의 분리

이지연,양시용,고은지,이승구,김시관,조왕식,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.2

본 연구가 VRSA에 대한 신규 항생물질 생산 균주를 얻기 위하여 실시하였다. 국내외에 채취한 토양시료에서 분리한 방선균 중, 임상분리 VRSA와 돌연변이 유발 VRSA에 대해 항균성을 나타내는 JY-24를 선별하였다. 선발 균주는 원통형의 포자 모양을 갖고 있으며, Bennett's 배지에 배양시 aerial mycelium color는 흰색을 나타냈으며, reverse side color는 대체로 노란색을 나타내었다. 항균물질 생산을 위한 탄소원으로는 fructose, mannose, 질소원으로는 yeast extract가 가장 우수하였으며 glucose의 첨가로 항균 물질 생산성이 증가하는 것으로 나타났다. 최적 초기 pH는 pH 7로 나타났으며, 28 ℃, 37℃, 45 ℃의 온도에서 균이 성장하는 것으로 나타났다. 또한 배양기간에 따라 항균물질 생산성을 조사한 결과, 임상분리 균주에 대해 5일 배양 시 가장 높았으나, 3일 배양과 7일 배양 시 동일한 생산성을 나타내 생산속도가 빠르며, 안정한 물질인 것이 파악되었다. 향후 균주동정 및 항균물질 정제에 관한 연구를 수행하고자 한다. An antibiotic substance producing strain JY-24, effective to the VRSA (Vancomycin-resistant Staphylococcus aureus), was isolated from soil. The spore chain of JY-24 was cylindrical, and its sulface was smooth. The aerial mass color of the strain was white, and its reverse side color was yellow. The strain did not produce soluble pigment. For the production antibiotics, fructose, mannose and yeast extract were favorable as carbon and nitrogen sources. Optimal initial pH for the production of the antibiotic was pH 7. Accumulation of the antibiotic in the culture broth reached at maximal level after 5 days cultivation in Bennett's medium.

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

Limulus Amoebocyte Lysate법에 의한 원유와 살균유의 세균수 측정

姜國熙,王智怨,朴興洙,李圭鍾,金敬民 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2

LAL method for the determination of Gram-negative bacterial Lipopolysaccharides(LPS) in milk is proposed and its value for bacteriological quality control is investigated. The relationship between the LPS contents and total colony count(cfu/ml), which covers the Gram-negative bacteria, was determined in raw milk samples. The coefficient of correlation between Gram-negative bacterial counts(cfu/ml) and LPS contents was r^2 =0.969. And, the correlation coefficient between total bacterial counts(cfu/ml) and LPS contents was r^2 =0. 914. It was also investigated how much the LPS contents were in UHT milks from l0 different dairy industrial companies in Korea. It varied from 92 ng LPS/ml to 938 ng LPS /ml.

전이성 신세포암 환자에서의 IFN-α와 5-FU를 이용한 복합 면역 화학 요법

김세웅,이지열,장광식,박왕진,정호석,조용현,윤문수 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

목적 : 전이성 신세포암의 경우 일반적으로 항암 화학요법, 방사선 치료 그리고 호르몬 치료 등의 다양한 치료 방법에도 불구하고 그 치료 성적이 불량하며 최근 진행된 전이성 신세포암이 치료에 사용되고 있는 면역요법과 면역화학요법의 치료 효과에 대한 많은 논란이 있다. 이에 저자들은 전이성 신세포암 환자들을 대상으로 시행한 interferon-α와 5-fluorouracil의 병용투여에 대한 면역화학요법의 치료 성적, 추적관찰 결과 및 부작용의 발현정도를 확인하여 그 임상적 유용성을 알아보고자 하였다. 방법 : 1995년 3월부터 2000년 2월까지 가톨릭 대학 부속 성모 병원에서 치료를 받았던 진행성, 전이성 신세포암 환자들 중 interferon-α와 5-fluorourcail의 복합면역화학요법을 시행 받고 추적관찰이 가능했던 총 17명의 환자를 대상으로 하였다. 대상 환자들에 대해 28일을 주기로 5-fluorouracil 750㎎/㎡(Body surface area)을 5일간 지속성 정맥 주사로 투여한 후 주 3회 interferon-α 300만 unit를 4주간 피하 주사하였다. 이들에 대해 8주마다 전산화 단층촬영, 동위원소 골주사, 흉부단순 촬영 등의 영상 진단을 통한 정기적인 추적 관찰을 시행하고 이들의 치료 성적, 추적관찰 결과 및 부작용 등에 대해 알아보았다. 결과 : Interferon-α와 5-Fluorouracil의 복합면역화학요법을 이용한 전체 17명의 전이성 신세포 암 환자의 경우 반응율은 완전관해는 없었으나 부분관해를 보인 경우가 2명(11.8%), 안정상태 유지는 6명(35.3%)이었다. 폐전이의 경우 복합면역화학요법에 대해 비교적 좋은 반응을 기대할 수 있었으며 이들의 생존 기간의 중앙값은 13.8개월이었다. 거의 모든 환자에서 미열을 동반한 전신 증상과 전신 쇠약감을 관찰 할 수 있었고 그 밖에 오심, 구토 및 설사 등의 소화기 증상과 두통 등의 경미한 부작용을 일부 환자에서 관찰할 수 있었으나 백혈구 감소증, 혈소판 감소증과 간기능 이상 등의 심각한 부작용을 보인 경우는 극히 적었다. 결론 : 전이성 신세포암은 비교적 그 예후가 불량하며 동시에 효과적인 치료 방법이 명확히 알려지지 않은 상태이다. 이러한 전이성 신세포암의 치료에 대한 interferon-α와 5-fluorouracil의 복합면역화학요법은 다른 종양의 치료에 비해 비교적 낮은 반응률과 고비용이 문제점으로 생각되나 비교적 부작용이 적은 안전한 치료 방법이므로 전이상 신세포암 환자에 있어 시행해 볼 가치가 있는 치료 방법의 하나로 사료된다. Background : Renal cell carcinoma is relatively resistant to both chemotherapy and immunotherapy. In this study, we evaluated and verified the efficacy and toxicity of therapies using interferon-α plus 5-fluorouracil in patients with metastatic renal cell carcinoma. Methods : A total of 17 patients with metastatic renal cell carcinoma were treated with interferon-α (3 million units/day. 3 times/week) subcutaneously plus 5-fluorouracil (750㎎/㎡ /day for 5 days) intravenously every 28 days from March 1995 to February 2000. We studied the response rate and the side effects of this regimen. Results : Among 17 patients treated with interferon-a plus 5-fluorouracil, 2 patient with lung metastasis was achieved partial remission (11.8%) and 3 patient with lymph node metastasis, 2 patients with lung metastasis and I patient with lung and bone metastases were achieved stable disease (35.3%). I and 5 year survival rates and median survival duration were 55%, 7% and 13.8 months, respectively. There were only mild to moderate side effects. The most common side effects were flu-like symptom such as fever, chill and malaise followed by gastrointestinal symptom such as nausea, vomiting and diarrhea, headache. High grade toxicities such as severe neutropenia and thrombocytopenia were rare. Conclusion : Immunochemotherapy using interferon-α plus 5-fluorouracil were moderately effective with modest toxicity in metastatic renal cell carcinoma. The most favorable response can be expected in lung metastasis. Most of side effects were tolerable. The effective therapy in patients with metastatic renal cell carcinoma should be studied and evaluated by further large randomized trials.

Pathoembryogenesis of terminal myelocystocele: terminal balloon in secondary neurulation of the chick embryo

Lee, Ji Yeoun,Kim, Saet Pyoul,Kim, Shin Won,Park, Sung-Hye,Choi, Jung Won,Phi, Ji Hoon,Kim, Seung-Ki,Pang, Dachling,Wang, Kyu-Chang Springer-Verlag 2013 Child's nervous system Vol.29 No.9

Functional recovery after injury of motor cortex in rats: effects of rehabilitation and stem cell transplantation in a traumatic brain injury model of cortical resection.

Lee, Do-Hun,Lee, Ji Yeoun,Oh, Byung-Mo,Phi, Ji Hoon,Kim, Seung-Ki,Bang, Moon Suk,Kim, Seung U,Wang, Kyu-Chang Springer Verlag 2013 Child's nervous system Vol.29 No.3

<P>Experimental studies and clinical trials designed to help patients recover from various brain injuries, such as stroke or trauma, have been attempted. Rehabilitation has shown reliable, positive clinical outcome in patients with various brain injuries. Transplantation of exogenous neural stem cells (NSCs) to repair the injured brain is a potential tool to help patient recovery.</P>

Enlarging arachnoid cyst: a false alarm for infants

Lee, Ji Yeoun,Kim, Jin Wook,Phi, Ji Hoon,Kim, Seung-Ki,Cho, Byung-Kyu,Wang, Kyu-Chang Springer-Verlag 2012 Child’s nervous system Vol.28 No.8

Organoclay‐assisted interfacial polymerization for microfluidic production of monodisperse PEG‐microdroplets and in situ encapsulation of <i>E. coli</i>

Wang, Kye Won,Lee, Kyoung G.,Park, Tae Jung,Lee, Young‐,Chul,Yang, Ji‐,Won,Kim, Do Hyun,Lee, Seok Jae,Park, Jung Youn Wiley Subscription Services, Inc., A Wiley Company 2012 Biotechnology and bioengineering Vol.109 No.1

<P><B>Abstract</B></P><P>We developed a novel one‐pot synthetic strategy for preparing monodisperse polyethylene glycol diacrylate (PEGDA) microdroplets via organoclay‐assisted interfacial polymerization approach for <I>Escherichia coli</I> encapsulation. Based on the mechanism of spontaneous and rapid polymerization of PEGDA precursor solution with Mg‐organoclay, the prepared PEGDA microdroplets have uniform size and fine round shape, with size range of 74–118 µm. The size of microdroplets can be controlled through the changing continuous phase flow rate. Organoclay‐assisted polymerization method provides a unique environment to produce non‐toxic ways of fabricating microorganism encapsulated microdroplets and to prohibit microdroplets merge during the processes. Furthermore, we successfully carried out to entrap <I>E. coli</I> inside of the PEGDA microdroplets. <I>E. coli</I> expressing a green fluorescent protein shows a good viability inside the PEGDA microdroplets. The in situ microfluidic synthetic method provides a novel approach for the preparation of monodisperse PEGDA microdroplets via a one‐pot route. Biotechnol. Bioeng. 2012;109: 289–294. © 2011 Wiley Periodicals, Inc.</P>

Functionally-relevant Expressed Sequence Tags from the Biomarker Organism, Physa acuta Exposed to Cadmium

Ji Eun JEONG,Bharat Bhusan PATNAIK,Se Won KANG,Hee-Ju HWANG,So Young PARK,Tae Hun WANG,Eun Bi PARK,Je Bong LEE,Myung-Mo NAM,Yong Hun JO,Yeon Soo HAN,Jun-Sang LEE,Hong Seog PARK,Yong Seok LEE 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.10