항 Vancomycin 내성균성 항생물질을 생산하는 Streptomyces sp.AMLK-335의 배양조건

이기형,임대석,최경희,이경호,김창한,김정환 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-

본 연구는 새로운 항 vancomycin 내성균성 항생물질을 개발하기 위하여 실시되었으며, 전국 각지의 토양 시료로부터 분리한 방선균 중 vancomycin 내성균주인 K-98 Enterococcus faecium 642, K-98 Enterococcus faecium 637 균주에 대하여 항생물질을 생산하는 분리균주 AMLK-335를 선정하여 이 균주의 항생물질 생산에 대한 최적 발효 조건을 검토한 결과, 탄소원 첨가로는 glycerol(0.5%), 질소원 첨가로는 yeast extract(0.5%)가 가장 효과적으로 항생물질 생산에 이용되었고, 비타민을 첨가에 따른 항생물질 생산성의 차이는 크게 나타나지 않았으나, calcium pantothenate 첨가시 항균활성이 비교적 높아졌으며, 금속이온의 첨가는 K+, Pb+, Ba²+, NA²+ , Ca²+를 첨가하였을 때는 항생물질의 생산성이 비교적 높아졌으며, 그중 Ca²+ 첨가구가 가장 생산성이 높았으며, Fe²+ , Hg+의 금속이온을 첨가하였을 때는 항생물질 생산성이 감소하였으며, CaCO₃첨가는 비첨가구보다 높은 항생물질 생산성을 보였으나, 첨가량에 따른 영향은 대체적으로 없었다. pH의 변화에 따른 생산성은 전반적으로 pH가 항생 물질 생산에 영향을 미치지 못하였으나, pH 4.5∼6.0 부근에서는 비교적 높은 항생물질 생산성을보였으며, 통기량에 따른 항생물질 생산성은 통기량이 많을수록 항생물질 생산이 좋았다. 한편 배양기간에 따라 항생물질 생산성을 조사한 결과, 5일째에 가장 높은 항생물질 생산성을 나타내었으며, pH의 변화는 배양 초기에 하강하였다가 점차 상승하는 경향을 보였다. This study was carried out for examining antibiotic productivity of the strain Streptomyces sp. AMLK-335 producing anti-VRE antibiotic. According to different condition, the strain was cultured for 4 days at 27℃ and determined cell growth, pH value and antibiotic productivity using disk diffusion method. In the experiment, the strain Streptomyces sp. AMLK-335 was examined various culture conditions which was added 8 carbon sources, 12 nitrogen sources, 8 vitamins and 11 metal ions in each time and controlled concentration of CaCO₃, initial pH and aeration. From the studies of conditions for antibiotic production, antibiotic productivity was the best to use 0.5% glycerol as carbon source and 0.5% yeast extract as nitrogen source. When several vitamins were added to medium, there was little difference in antibiotic productivity. But the addition of calcium pantothenate slightly increased antimicrobial activity. The addition of metal ion, such as K+, Pb+, Ba²+, NA²+ and Ca²+ slightly increased antibiotic productivity except Fe²+ and Hg+. The addition of CaCo₃showed higher than non-addition experiment in antibiotic production, but there wasn't generally influenced at addition concentration. According to pH change, antibiotic productivity was increased at pH 4.5∼6.0, and when there was a lot of aeration, antibiotic productivity was increased.

칼슘제 수관살포가 참다래의 과실 품질과 저장에 미치는 영향

임경호,나양기,임동근,마경철,조윤섭,김월수,이상현,박용서 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

This study were carried out to improve Kiwifruit quality and storage life. Three kinds of calcium compound were sprayed and calcium content of fruits, weight loss during fruit storage and fruit quality were investigated. Calcium contents within leaves and fruit were lower in Clef-non treatment than that of control. The calcium content in fruit pericarp of Kalk-H and CaCl2 was 0.04 to 0.05% higher than that of control. Fruit weight and soluble solids content at harvest was a little higher but acidity and fruit hardness was lowered. Fruit weight loss of Kalk-H and CaCl2 treatment was 1.39 to 1.53% lower than that of control during storage. The soluble solids of ripen fruit was 1.0 to 1.3% higher in all treatment and 0.8% higher in Kalk-H treatment in 120 of after storage. Fruit hardness of control fruit was higher at harvest but that of CaCl2 treatmented fruit was 0.39㎏/φ 5㎜ higher in 120 days of storage.

專門大學海洋土木科模型敎育課程 修正開發硏究

李東周,林秉龍,張仁奎,申文燮,金榮燮,朴昌圭,金康洙 군산대학교 1988 群山水産專門大學 硏究報告 Vol.22 No.1

This is a study to modify the curriculum of the ocean civil engineering department in junior college. We collected and analyzed data which are concerned on the curriculum development or improvement, the marine techniques and the ocean policy. And then we also gathered the opinions of the professors serving at the ocean civil engineering department by holding several conferences and investigated the opinions of the ocean civil engineers serving at the industrial organization by way of the inquring papers. In addition we collected the advices of educational specialists and ocean civil specialists by visiting personally. On the basis of above opinions we developed the jobs of graduates and made a model curriculum shown in Table 6. We tried to intensify the subjects of special basic field related to the ocean & civil engineering and the subjects of basic experiment & practise. The ocean observation practise was newly formed and the E. D. P.S was reinforced.

도시지역 노인의 건강행위 실천 실태

이태진,배상수,이기홍,임재영,강경화 노인간호학회 2008 노인간호학회지 Vol.10 No.1

Purpose: The purpose of this study was to identify the health status and health behavior practices of elders living in urban areas. Methods: The participants were selected by convenience sampling and consisted of 137 elders 65 or over living in a city in Kyunggi Province. Data collection was done through interviews using a structured questionnaire. Results: Of the participants. 66.4% perceived their health status as 'not healthy'. The average number of present illnesses reported by the elders was 1.7. The average scores for health behavior practices were low (smoking cessation: 1.64, drinking abstinence: 1.79, exercise: 0.40, regular diet: 1.54, weight control: 1.67, regular physical check-up: 0.77). The scores for health behaviors practices showed significant differences according to general characters and health status. Conclusion: Health promotion programs for elders should be designed to enhance health behaviors practices.

광에너지 총량에 따른 광중합 콤포짓트레진과 콤포머의 중합도에 관한 연구

황경란,이용근,임범순,김철위 대한치과기재학회 2000 대한치과재료학회지 Vol.27 No.4

Dental composite resin is filler added, particle reinforced composite material, and the usage range had been enlarged in the posterior restoration through improvement of physical properties. Recently, resin modified glassionomer and compomer are used for various purposes. The degree of polymerization of cross-liked polymer has great importance in determining the mechanical properties and clinical availability of materials. Residual carbon-carbon double bonds have been implicated in causing reduced wear resistance, strength and color stability. The degree of polymerization is an important factor for longevity of a restoration. However the higher the degree of polymerization in resin composites, the higher the shrinkage will result. It has been elucidated that the degree of polymerization was significantly affected by the intensity of the curing light, wavelength of light and irradiation time. In this experiment, 16 kinds of restorative materials such as five kinds of universal composite resin, five kinds of condensable composite resin, two kinds of flowable composite resin, and four kinds of compomer were used. This study examined the effect of light intensity and irradiation time on the polymerization behavior such as the heat release during polymerization and the rate of polymerization by DSC (differential scanning calorimeter), and the degree of double bond conversion by FT-IR. With DSC, the irradiation modes were three different light intensity of 300, 400, 600 ㎽/㎠ and for 40 seconds. With FT-IR, the levels of irradiation energy were 1,500, 40,000, 8,000, 16,000 and 24,000 mJ/㎠ by combining the light intensity of 300, 400, 600 ㎽/㎠ and irradiation time of 5, 10, 20 and 40 seconds. From this experiment, The following results were obtained : 1.In the DSC result, heat of polymerization of composite resin was 23.22∼55.17J/gram and exothermic energy of compomer was 26.50∼52.68 J/gram 2.At the isothermal condition of 27℃, there were exothermal peaks at 40.812∼44.724 seconds from the start of irradiation. The percentage of polymerization by released heat was 19.12∼49.44% after 18 seconds, 37.23∼82.07% after 36 seconds, and 93.57∼98.53% after 54 seconds from the start of irradiation. 3.From FT-IR, the degree of polymerization of composite resin was 17.17∼60.91% and that of compomer was 7.74∼40.82%, and the degree of final conversion was influenced by the kinds of restorative materials and the irradiation energy(p<0.01). 4.As the irradiation energy was increased from 1,500 mJ/㎠ to 16,000 mJ/㎠, the degree of conversion was increased also. However, the degree of final conversion was no significant differences with the irradiation energy of 24,000 mJ/㎠ and 16,000 mJ/㎠(p>0.05). The wavenumber of absorbance peak of aromatic double bond was similar regardless of the restorative materials, and appeared around 1606.67∼1610.67 cm-1. The absorbance peak of aliphatic double bond appeared around 1636.64∼1638.52 cm-1 from FT-IR.

【영아보육프로그램】자료 개발 연구

황해익,임재택,김정신,송연숙,이경화,김남희 부산대학교 영유아보육연구소 1998 영유아보육연구 Vol.4 No.-

This study aimed to develop Materials for (The Infant Educare Program) (Dept. of Health & Welfare, 1996). Infant educare Materials were developed through following procedures: 1) analysis of reference and other educare materials 2) content analysis of (The Infant Educare Program) 3) developing materials and 4)testing and revising materials by professionals. Infant educare Materials that developed in this study were constituted of 12 kinds in 5 areas - Materials for management of daycare center, the nursery stories for infants, musical materials for infants, play things for infants, and educational materials for parents. First, materials for management of daycare center were considered various conditions whithin daycare centers and were compased of 2 kinds - diary for educare plan and diskettes for documents. Second, the nursery stories for infants were compased of materials for 3 stages-the weaning stage, the toddling stage, and the independent stage. Similarly, musical materials for infants were composed of 3 stages. And play things for infants were composed of 2 kinds - play things to be easily founded and easily making play things. Finally, educational materials for parents were composed of 2 kinds - guide book for parents education and diskettes for communication with parents. It is needed that persistent attention from professionals in developing and testing materials for infant educare. Also, further educational implications were discussed

비가림 및 망사 시설재배가 참다래의 수체생육 및 과실품질에 미치는 영향

나양기,마경철,임동근,김병삼,임경호,김월수,이상현,박용서 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

This studies were conducted to investigate the effects of rain shelter and netting on the tree growth, fruit set fruit quality of kiwifruit (Actinidia deliciosa). In rain shelter and net house, shoot falling wasn't occurred by strong wind, and fruit set, fruit weight and also fruit malformation, side flowering increased at the same time. In rain shelter house the increment temperature forced blooming date and increased shoot length, leaf number and diameter but in net house these tendencies were not observed. Relative light intensity of rain shelter house was higher than that of net house with standard of field culture (relative light intensity: 100%). Soluble solid content was higher in rain shelter house, and acid content was higher in net house. With the effect of rain sheltering the occurrence of bacterial bloom rot and canker significantly decreased in both rain shelter and net house but insect injury increased. These data showed that rain shelter house is more suitable for kiwi fruit culture.

Assessment of Parenting Attitudes by Children and Adolescents with Migraine

Kyeong Mi Lee(Kyeong Mi Lee),Min Suk Koh(Min Suk Koh),Ho-chan Lim(Ho-chan Lim),Soorack Ryu(Soorack Ryu),Yong Joo Kim(Yong Joo Kim),Jin-Hwa Moon(Jin-Hwa Moon) 대한소아신경학회 2022 대한소아신경학회지 Vol.30 No.4

Purpose: We aimed to investigate the parenting attitudes reported by patients and their relationships with the characteristics of headaches in children and adolescents with migraine. Methods: We conducted a retrospective review of medical records of children and adolescents with migrainous headaches (n=115; 59.1% female; mean age, 11.89±2.00 years). Children evaluated parental attitudes using the Parenting Attitude Test-Youth (PAT-Y), which comprises eight subscales and four newly devised secondary subscales. Headache severity was calculated by the visual analog scale (VAS), monthly frequency (MF), and VAS×MF/4 (VF). The scores of PAT-Y subscales and the correlations between PAT-Y scores and headache severity were analyzed by age group and sex. Scores for children’s depression inventory, childhood behavior checklists, and an attention deficit hyperactivity disorder scale were also analyzed. Results: In the elementary school age group, VAS was weakly negatively correlated with the “achievement press” (r=-0.28, P<0.05) and “high expectation” (r=-0.25, P<0.05) attitudes, and VF was weakly negatively correlated with “achievement press” (r=-0.32, P<0.05), “punishment” (r=-0.27, P<0.05), and “high expectation” (r=-0.29, P<0.05). In the middle-school age group, MF and VF were moderately positively correlated with the “achievement press” attitude (r=0.48, P<0.01 and r=0.48, P<0.01, respectively), VF was weakly positively correlated with the “neglectful” attitude(r=0.31, P<0.05), and MF was weakly positively correlated with scores for depression (r=0.29, P<0.05) and internalized problems (r=0.31, P<0.05). Conclusion: Parenting attitudes perceived by children and adolescents with migrainous headaches varied by age, and some parenting attitudes were related to headache severity. Education on age-appropriate parenting attitudes may help cope with migrainous headaches.

Bootstrap method to evaluate tightness of clusters with application to the Korean standard occlusion study

Lim, Johan,Lee, Sungim,Park, Heon-Jin,Eun Lee, Kyeong,Lee, Shin-Jae Taylor Francis 2014 JOURNAL OF STATISTICAL COMPUTATION AND SIMULATION Vol.84 No.2

Improved real-time PCR method for quantification of the abundance of all known ribotypes of the ichthyotoxic dinoflagellate <i>Cochlodinium polykrikoides</i> by comparing 4 different preparation methods

Lee, Sung Yeon,Jeong, Hae Jin,Seong, Kyeong Ah,Lim, An Suk,Kim, Ji Hye,Lee, Kyung Ha,Lee, Moo Joon,Jang, Se Hyeon Elsevier Science B.V., Amsterdam. 2017 HARMFUL ALGAE Vol.63 No.-

<P><B>Abstract</B></P> <P>Red tides by the ichthyotoxic dinoflagellate <I>Cochlodinium polykrikoides</I> have caused large scaled mortality of fish and great loss in aquaculture industry in many countries. Detecting and quantifying the abundance of this species are the most critical step in minimizing the loss. The conventional quantitative real-time PCR (qPCR) method has been used for quantifying the abundance of this species. However, when analyzing >500 samples collected during huge <I>C. polykrikoides</I> red tides in South Sea of Korea in 2014, this conventional method and the previously developed specific primer and probe set for <I>C. polykrikoides</I> did not give reasonable abundances when compared with cell counting data. Thus improved qPCR methods and a new specific primer and probe set reflecting recent discovery of 2 new ribotypes have to be developed. A new species-specific primer and probe set for detecting all 3 ribotypes of <I>C. polykrikoides</I> was developed and provided in this study. Furthermore, because the standard curve between cell abundance and threshold cycle value (Ct) is critical, the efficiencies of 4 different preparation methods used to determine standard curves were comparatively evaluated. The standard curves were determined by using the following 4 different preparations: (1) extraction of DNA from a dense culture of <I>C. polykrikoides</I> followed by serial dilution of the extracted DNA (CDD method), (2) extraction of DNA from each of the serially diluted cultures with different concentrations of <I>C. polykrikoides</I> cultures (CCD method), (3) extraction of DNA from a dense field sample of <I>C. polykrikoides</I> collected from natural seawater and then dilution of the extracted DNA in serial (FDD method), and (4) extraction of DNA from each of the serially diluted field samples having different concentrations of <I>C. polykrikoides</I> (FCD method). These 4 methods yielded different results. The abundances of <I>C. polykrikoides</I> in the samples collected from the coastal waters of South Sea, Korea, in 2014–2015, obtained using the standard curves determined by the CCD and the FCD methods, were the most similar (0.93–1.03 times) and the second closest (1.16–1.33 times) to the actual cell abundances obtained by enumeration of cells. Thus, our results suggest that the CCD method is a more effective tool to quantify the abundance of <I>C. polykrikoides</I> than the conventional method, CDD, and the FDD and FCD methods.</P>