동측 대퇴골 경부 및 간부 골절의 치료 : 15례 분석

정필현,강석,채동주,홍정룡,김종필 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

동측 대퇴골 경부 및 간부골절은 드문 골절로서 초진시 경부골절을 간과하는 경우도 있고 치료에 있어서도 여러 가지 방법이 제시되어져 왔다. 저자들은 1989년 6월부터 1996년 5월까지 경험한 동측 대퇴골 경부 및 간부골절 환자 15례를 대상으로 다양한 방법으로 치험한 예를 분석하였다. 15례중 8례에서 경부골절과 간부골절을 동시에 고정하는 재건금속정으로 치료하였고 전례에서 골유합을 얻었으며 평균 골유합기간은 경부골절 14주, 간부골절 20.5주였다. Ipsilateral femoral neck and shaft fractures present diagnostic difficulties and complex choices as to treatment. We report the result of treating 15 such fractures. High-energy accidents accounted for most of these injuries. There were numerous associated injuries, many requiring operative procedures. Eight of Fifteen patients were treated with Reconstruction interlocking nails. All fractures united. There was no delay in diagnosis of femoral neck fractures, and all healed without avascular necrosis. The use of the reconstruction nail is technically demanding. We conclude that this device gives good results for these fractures.

탱자(Poncirus tripoliata RAF)잎으로부터 Mitochondrial DNA의 분리

박종필,전홍성,김연순,이숙영,김성준 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-

Differaitial centrifugation, sucrose(30%, 60%) and percoll(13.5%, 21% and 45%) density gradient centrifugation were used to effectively isolate mitochondria and mitochondrial DNA (mt DNA) from the leaves of the genus Poncirus (Pondrus trifoliata RAF.) The results showed that percoll density gradient centrifugation provided a convinient and effective method for the preparation of clean mitochondria. The purity of prepared mt DNA was increased by the removal of the nuclear DNA by DNase treatment to the crude mitochondrial suspension mt DNA on the agarose gel was shown extra fluorescent background in case of isolation by differential centrifugation and more cleared band included slightly smeared patterns for sucrose density gradient, and highly paint band from percoll density gradient was appeared to the best results of the above three methods. In addition to chromosomal mt DNAs, two short mt plasmid-like elements were detected in the genus Poncirus. These elements consistently co-purified with the chromosomal mt DNAs and is widely distributed among natrual Poncirus trifoliata RAF. populations. But these elements required proteinase K treatment for mobility in agarose gels.

대퇴동맥을 통한 경피적 관동맥 중재시술 환자의 천자부위 지혈을 위한 Angioseal^(�) 사용과 고식적 용수 압박법의 비교 : 전향적 연구

김용훈,권현철,김필호,안석진,유철웅,최진호,이상철,김준수,김덕경,전은석,이상훈,홍경표,박정의,서정돈 대한내과학회 2004 대한내과학회지 Vol.66 No.5

목적 : 경피적 관동맥 중재술은 최근 양적 및 질적으로 급격한 발전을 보였지만 시술 시 천자부위의 혈관 합병증은 아직 해결해야 할 문제점이다. 저자들은 대퇴동맥을 통한 경피적 관동맥 중재술을 환자에게 천자부위 지혈을 위한 혈관폐쇄기구인 안지오실의 안전성과 유용성을 고식적인 용수 압박법과 비교 연구하고자 하였다. 방법 : 2002년 4월부터 2003년 5월 사이에 삼성서울병원 심장혈관센터에서 대퇴동맥을 통한 경피적 관동맥 중재술을 성공적으로 시행한 200명의 환자(안지오실 사용군: A군, 100명, 고식적 용수압박법 사용군 B군, 100명)를 대상으로 시술 후 주요 합병증 및 경한 합병증, 지혈 후 환자가 자리에 앉기까지의 시간, 보행개시 가능시간, 총 재원 기간과 시술 1주 후의 합병증을 전향적으로 조사하여 비교 연구하였다. 결론 : 두 군에서 연령, 성별, 기저질환, 심혈관 질환의 위험요소, 시술의 종류, 시술 중 사용한 헤파린의 양, clopidogrel의 양, ticlopidine의 양, 지혈 시 수축기와 확장기혈압, ACT (activated clotting time)는 차이가 없었다. A군이 B군에 비해 시술 후 자리에 앉기까지의 시간 (A군: 4.3±0.3시간, B군: 13.7±0.8시간, p=0.004) 및 보행개시까지의 시간(A군: 6.8±0.5시간, B군: 18.8±2.1시간, p=0.013)이 유의하게 짧았다. 시술 후 주요 합병증은 두군 모두에서 관찰되지 않았으며, 경한 합병증의 전체발생은 A군에서 유의하게 적었다(A군: 28명, B군: 19명, p=0.003). 반상출혈의 경우는 A군에서 유의하게 낮았지만(A군: 3명, B군: 12명, p=0.01), 혈종, 출혈의 발생은 두군간에 차이가 없었다. 총 재원기간에는 두 군간에 차이가 없었다(p=0.239). 시술 1주 후 경과관찰에서 주요합병증은 역시 두 군 모두에서 관찰되지 않았으며, 경한 합병증의 전체발생은 두 군에서 차이가 없었다(A군: 15명, B군: 13명 p=0.418), 반상출혈의 빈도는 차이가 없었지만 혈종의 발생은 A군에서 더 낮았다(A군: 2명, B군:6명, p=0.004). 두 군 모두에서 출혈은 발생하지 않았다. 결론 : 대퇴동맥을 통한 경피적 관동맥 중재술을 시행한 환자에서 안지오실의 사용은 고식적 용수 압박법에 비해 환자의 침상 안정시간을 줄여 조기 활동개시가 가능하게 하였으며 국소합병증의 위험도를 일부 낮추어, 시술에 따른 환자의 불편을 현저하게 감소시킬 수 있다고 할 수 있을 것으로 기대된다. Background : Although the number and the quality of percutaneous coronary intervention have been recently increased dramatically, the vascular complication at puncture site is still the major cause of patients' morbidity. We evaluated the safety and efficacy of newly of newly-developed collagen-based arterial closure device, Angioseal after transfemoral percuatenous coronary intervention. Methods : This study was designed as a prospective single center non-randomized comparative study. A total 200 patients undergoing transfemoral percutaneous coronary intervention were enrolled between April 2002 and May 2003. They were divided into two groups; Angioseal group (group A, n=100) and manual compression group (group B, n=100). The baseline clinical and angiographic characteristics were reviewed. The time to sit up, the time to ambulation, the duration of hospital stay, major and minor vascular puncture site complications were monitored. The patients were followed-up for 1 week after procedure by telephone. Results : The baseline clinical characteriwtics, clinical diagnosis, cardiocascular risk factors, typesof procedure, doses and numbers of anticoagulants were similar between two groups. The blood pressure and activated clotting time were also similar. The time to sit up (group A: 4.3±0.3 hours, group B: 13.7±0.8 hours, p=0.004) and the time to ambulation (group A: 6.8±0.5 hours, group B: 18.8±2.1 hours, p=0.013) were shorter in group A. No major vascular complications were noted. The incidence of hematoma and bleeding were not significantly different between two groups. The incidence of ecchymosis, however, was significantly lower in group A (group A:12%, group B: 3%, p=0.001) The duration of gospital stay was similar between groups. During 7 days of follow-up period incidence of hematoma was significantly lower in group A (p=0.004). Although the incidence of ecchymosis was not significantly different between two groups. Conclusion : The angioseal may be associated with earlier ambulation and less patients' morbidity with low incidence of local complication rate compared to manual compression after transfemoral percutaneous coronary intervention.

Anti-cancer effect of Eriocaulon sieboldianum through the activation of caspase-3 in human leukemia cell line, HL-60 cells

Kim, Su-Jin,Lee, Gi-Tak,Lee, Bo-Ra,Jeon, Kwon-Su,Rim, Hong-Kun,Bang, Jun-Ho,Kim, Yang-Gwi,Myung, No-Yil,Moon, Phil-Dong,Kim, Na-Hyung,Choi, In-Young,Choi, Young-Jin,Kang, In-Cheol,Um, Jae-Young,Hong, Kyung Hee Oriental Medicine Research Center 2009 Oriental pharmacy and experimental medicine Vol.9 No.2

Eriocaulon sieboldianum (ES) is used in traditional oriental medicine for various medicinal purposes including headache, toothache, and inflammation. However, the anti-cancer effect of the ES is still not fully understood. In the present study, the human leukemia cell line HL-60 was used to characterize the apoptotic effects of ES. ES induced cytotoxicity of HL-60 cells in a dose- and time-dependent manner. ES induced the generation of reactive oxygen species, and the release of cytochrome c in a dose-dependent manner. In addition, we showed that ES-induced apoptosis was accompanied by activation of caspase-3. Taken together, our results demonstrate that ES possesses anti-cancer activity in HL-60 cells.

Solitary Keratoacanthoma at the Recipient Site of a Full-Thickness Skin Graft: A Case Report and Review of the literature

Kim JeongHo,Lee Sang-Hoon,Kim Jiye,Hong Seung-Phil,Kim Sug Won 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.1

A 57-year-old man presented with a pigmented papule, 0.4 cm in diameter, on the left lower eyelid. Skin biopsy revealed a basal cell carcinoma, which was excised through a wide excision followed by a full-thickness skin graft (FTSG). Two weeks after the surgery, an erythematous nodule developed in the lower margin of the graft recipient site. The nodule size increased rapidly over 2 weeks, becoming dome-shaped with a central hyperkeratotic plug. A diagnosis of keratoacanthoma (KA) was made, and surgical excision was performed. Histological findings revealed a large, well-differentiated squamous tumor with a central keratin-filled crater and buttress. The HPV genotyping results were negative. Risk factors for KA include trauma, old age, exposure to UV radiation, immunosuppression, and HPV infection. KA has most often been reported to develop at the donor site. Although the pathogenesis of KA is unclear, trauma is believed to act as a second insult to a preceding oncogenic insult, such as exposure to UV radiation, resulting in a koebnerization. Herein, we report a case of solitary KA at a FTSG recipient site. This report presents information that may provide guidance during dermatologic surgeries.

Stream health, topography, and land use influences on the distribution of the Eurasian otter <i>Lutra lutra</i> in the Nakdong River basin, South Korea

Hong, Sungwon,Kim, Dong-Kyun,Do, Yuno,Kim, Ji Yoon,Kim, Young-Min,Cowan, Phil,Joo, Gea-Jae Elsevier 2018 Ecological Indicators Vol.88 No.-

<P><B>Abstract</B></P> <P>In South Korea, Eurasian otter (<I>Lutra lutra</I>) habitat has increased since the 1990 s because of legal protection, forest development, and increased public awareness. Although otters have been detected throughout the country, a precise investigation of habitat conditions has not previously been undertaken. We hypothesized that higher values of stream health assessment programme (SHAP) indices would indicate better habitat for otters. Otter distribution based on spraint density was investigated along 600 m riverside transects at 250 sites in the Nakdong River basin. We defined four otter population categories (absent, rare, normal, and abundant) and compared the results with presence and absence data. Use of the four population categories provided more details about habitat conditions than presence and absence analysis. The highest spraint densities were found at higher altitudes, and densities declined in areas with high human density and urbanization. The benthic macro-invertebrate community index was highly positively related to the otter population indices. In terms of fish as prey items, the proportion of species tolerant to environmental change were negatively related to otter indices, otherwise number of species sensitive to environmental change positively related. Overall, stream health assessment indices provided the highest correlations with otter population numbers and distribution.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Habitat quality was investigated using the stream health assessment program (SHAP). </LI> <LI> We hypothesized that higher SHAP indices would indicate better habitat for otters. </LI> <LI> Benthic macro-invertebrate and otter population indices were highly related. </LI> <LI> Otter population indices provided better habitat information comparing with presence-absence data. </LI> <LI> Stream health assessment indices showed best correlation with otter distribution. </LI> </UL> </P>

Predictors of mortality in Middle East respiratory syndrome (MERS)

Hong, Ki-Ho,Choi, Jae-Phil,Hong, Seon-Hui,Lee, Jeewon,Kwon, Ji-Soo,Kim, Sun-Mi,Park, Se Yoon,Rhee, Ji-Young,Kim, Baek-Nam,Choi, Hee Jung,Shin, Eui-Cheol,Pai, Hyunjoo,Park, Su-Hyung,Kim, Sung-Han British Medical Association 2018 Thorax Vol.73 No.3

<P>We evaluated the clinical characteristics, cytokine/chemokine concentrations, viral shedding and antibody kinetics in 30 patients with Middle East respiratory syndrome (MERS), including 6 non-survivors admitted to 3 MERS-designated hospitals. Old age, low albumin, altered mentality and high pneumonia severity index score at admission were risk factors for mortality. In addition, severe signs of inflammation at initial presentation (at hospital days 1-4), such as high inducible protein-10 (p=0.0013), monocyte chemoattractant protein-1 (p=0.0007) and interleukin 6 (p=0.0007) concentrations, and poor viral control (high viral load at hospital days 5-10, p<0.001) without adequate antibody titres (low antibody titre at hospital days 11-16, p=0.07) during the course of disease, were associated with mortality.</P>

The anti-allergy and anti-inflammatory effect of Anemarrhenae Rhizoma in vivo and in vitro

Kim, Su-Jin,Jeong, Hyun-Ja,Myung, Noh-Yil,Moon, Phil-Dong,Lee, Ju-Young,Yi, Byoung-Jae,Lee, Eun-Hyub,An, Nyeon-Hyung,Park, Seok-Jae,Kim, Min-Cheol,Jun, Suk-Min,Lee, Ji-Hyun,Kim, Hyung-Min,Hong, Seung- Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.3

Anemarrhenae Rhizoma (AR) is used in traditional oriental medicine for various medicinal purposes. However, the exact mechanism that accounts for the anti-allergy and anti-inflammatory effects of the AR is still not fully understood. The aim of The present study is to elucidate whether and how AR modulates the allergic reactions in vivo, and inflammatory reaction in vitro. In this study, we showed that AR significantly decreased compound 48/80-induced systemic anaphylaxis, paw oedema, and histamine release from preparation of rat peritoneal mast cells. Also, AR inhibited the expression of inflammatory cytokine in PMA plus A23187-stimulated human mast cells (HMC-1). In addition, we showed that anti-inflammatory mechanism of AR is through suppression of nuclear factor-${\kappa}B$ activation $I{\kappa}B-{\alpha}$degradation. These results provided new insight into the pharmacological actions of AR as a potential molecule for therapy of inflammatory allergic diseases.

Adiponectin Enhances Human Keratinocyte Lipid Synthesis via SIRT1 and Nuclear Hormone Receptor Signaling

Hong, Seung-Phil,Seo, Hee-Seok,Shin, Kyong-Oh,Park, Kyungho,Park, Byung Cheol,Kim, Myung Hwa,Park, Myungjin,Kim, Chang-Deok,Seo, Seong Jun Elsevier 2019 The Journal of investigative dermatology Vol.139 No.3

Combination Therapy with a PI3K/mTOR Dual Inhibitor and Chloroquine Enhances Synergistic Apoptotic Cell Death in Epstein-Barr Virus-Infected Gastric Cancer Cells

Kim, Mi-Young,Kruger, Annie J.,Jeong, Ju-Yeon,Kim, Jaehee,Shin, Phil kyung,Kim, Sun Young,Cho, Joo Young,Hahm, Ki Baik,Hong, Sung Pyo Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.6

The phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway is a promising target for gastric cancer (GC) treatment; however the efficacy of PI3K/mTOR dual inhibitors in GC has not yet been maximized. Additionally, the effect of autophagy regulation by PI3K/mTOR dual inhibitors has not been clearly elucidated in GC treatment. We aimed to show that our newly developed PI3K/mTOR dual inhibitor, CMG002, when combined with an autophagy inhibitor, chloroquine (CQ), potently induces effective cancer cell death in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) cells, where both the PI3K/AKT/mTOR and autophagy pathways play important roles in disease pathogenesis. EBV- and mock-infected AGS and NUGC3 GC cell lines were treated with CMG002 +/- CQ. PI3K/AKT/mTOR signaling pathway mediators, cellular apoptosis and autophagy markers were confirmed by Western blot assay. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay. CMG002 effectively blocked the PI3K/AKT/mTOR pathway by markedly decreasing phosphorylation of AKT and its downstream mediator S6. CMG002 induced G0/G1 cell cycle arrest and enhanced apoptotic cell death in AGS and NUGC3 cells, particularly EBV-infected cells compared with mock-infected cells, as confirmed by flow cytometric analyses and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays. The combination of CMG002 plus CQ synergistically increased apoptotic cell death in EBV-infected GC cell lines when compared with CMG002 alone (P < 0.05). Our results suggest that the new PI3K/mTOR dual inhibitor, CMG002, when used in combination with the autophagy inhibitor, CQ, provides enhanced therapeutic efficacy against EBVaGC.