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성견의 2급 치근 분지부 결손에서 자가골막 이식에 의한 치주조직 재생
남승지,정현주,김영준 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1
Autogenous periosteal grafts are the attractive alternative to existing barrier membrane materials since they meet the reqiurements of an ideal material. But no histological data are available on the effectiveness of periosteal membranes in the treatment of periodontal defects. The purpose of this study was to evaluate effect of autogenous periosteal graft on periodontal regeneration histologically. Calss Ⅱ furcation defects were surgically created on the second, third and the fourth premolars bilaterally in the mandibles of six mongrel dogs. The experimental sites were divided into three groups according to the treatment modalities; control group - surgical debridement only : Group Ⅰ- autogenous periosteal membrane placement after surgical debridement; Group Ⅱ- autogenous periosteal membrane placement after surgical debridement and bone grafting. The animals were sacrificed at 2, 4 and 12 weeks after periodontal surgery and the decalcified and undecalcified specimens were prepared for histological and histometrical analysis. Clinically all treated groups healed without significant problems. Under light microscope, at 2 weeks, control group showed significant apical epithelial migration and bone remodelling only below the notch area. But for the groupⅠ, Ⅱ with autogenous periosteal graft, less apical migration of epithelium appeared and large amount of osteoid tissue was showen above the notch area. Grafted periosteal membrane was indiscernable at 4 weeks, so periosteal membrane might be organized to surrounding tissues. Histometrically, at 4 and 12 weeks, all the test and control groups didn't show significant change of epithelial zone but new attachment level tended to be gained in the test groups than control group. These results suggest that autogenous periosteal grafts could be a good alternative for guided tissue regeneration.
제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산
이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1
재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.
The MCP-1/CCR2 axis in podocytes is involved in apoptosis induced by diabetic conditions.
Nam, Bo Young,Paeng, Jisun,Kim, Seung Hye,Lee, Sun Ha,Kim, Do Hee,Kang, Hye-Young,Li, Jin Ji,Kwak, Seung-Jae,Park, Jung Tak,Yoo, Tae-Hyun,Han, Seung Hyeok,Kim, Dong Ki,Kang, Shin-Wook Rapid Science Publishers ; Kluwer Academic Publish 2012 Apoptosis Vol.17 No.1
<P>Previous studies have demonstrated the importance of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of diabetic nephropathy in terms of inflammation, but the direct role of the MCP-1/CCR2 system on podocyte apoptosis under diabetic conditions has never been explored. In vitro, mouse podocytes were exposed to a medium containing 30?mM glucose (HG) with or without CCR2 siRNA or CCR2 inhibitor (RS102895). Podocytes were also treated with MCP-1 or TGF-β1 with or without anti-TGF-β1 antibody, CCR2 siRNA, or CCR2 inhibitor. In vivo, 20?db/m and 20?db/db mice were divided into two groups, and ten mice from each group were treated with RS102895. Western blot and Hoechst 33342 or TUNEL staining were performed to identify apoptosis. HG-induced apoptosis and TGF-β1 levels were significantly abrogated by CCR2 inhibition. In addition, treatment with MCP-1 directly induced apoptosis via CCR2. Moreover, TGF-β1- and MCP-1-induced apoptosis were significantly ameliorated by the inhibition of CCR2 and anti-TGF-β1 antibody, respectively. Glomerular expression of cleaved caspase-3 and apoptotic cells within glomeruli were also significantly increased in db/db mice compared to db/m mice, and these increases were significantly attenuated in db/db?+?RS102895 mice. These results suggest that interactions between the MCP-1/CCR2 system and TGF-β1 may contribute to podocyte apoptosis under diabetic conditions.</P>
( Ji Won Gye ),( Ji Yeon Yoo ),( Sung Ku Ahn ),( Ji Eun Kwon ),( Chan Hee Nam ),( Ji Seok Kim ),( Jee Young Kim ),( Byung Cheol Park ),( Myung Hwa Kim ),( Seung Phil Hong ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Non-melanoma skin cancers are caused mainly by prolonged UV exposure. However, there is no effective prevention other than avoiding sun exposure. Recently, ablative fractional laser(FL) treatment is actively being carried out for facial rejuvenation. Objectives: We elucidated whether the occurrence of skin tumors caused by exposure to UV can be decreased by multiple sessions of ablative CO2 FL. Methods: Two groups of hairless mice were treated with either ablative CO2 FL or nothing at 3-week intervals during the 20 weeks of UV exposure period. At the 30-week, representative tumors were taken to evaluate the type of tumor and check the mRNA expression levels of the MMP-13 and type-1 procollagen. In addition, we evaluated the change of skin barrier function at 12 weeks and 20 weeks. Results: At 30 weeks, the UV plus FL treated group(group I) showed a significantly lower average size of tumor and a lower occurance rate of tumors than the UV only treated group(group II). Tumors in the group II showed more malignant progression. And the groups II showed significantly greater trans-epidermal water loss than the group I. The group II showed significantly decreased collagen and elastic contents than the group I. The group I presented a higher mRNA level of type-1 procollagen, and a lower level of MMP-13 than the group II. Conclusion: Ablative CO2 FL can be effective for not only skin rejuvenation but also for prevention of skin tumors induced by UV.