스티렌 폭로 근로자들의 기중 및 혈중 스티렌과 요중 만델산의 관계 분석

정호근,강성규,양정선,김기웅,이종성,조영숙,박인정 大韓産業醫學會 1994 대한직업환경의학회지 Vol.6 No.1

The concentration of styrene in air in blood and mandelic acid in urine were checked for the 60 workers with normal liver function, exposed to styrene. Styrene in air were sampled with personal air sampler at least 4 hours and analyzed by gas chromatography. Blood and spot urine were collected at the end of shift with a vacuum tube and a polythylene bottle and analyzed by gas chromatography and high performance liquid chromatography. Means of air and blood styrene and urine mandelic acid were 8.16 ppm(geometric mean), 0.199 mg/L, and 0.519 g/g creatinine, respectively. The concentration of styrene in air and mandelic acid in urine were high in the FRP factories and low in polymerization factory. Styrene in blood showed large difference by the working process. Styrene in air showed a good correlation with mandelic acid in urine(r=0.6369) and styrene in blood(r=0.6371). The mandelic acid in urine and styrene in blood corresponded to exposure of 50 ppm styrene were 0.890 g/g creatinine and 0.434 mg/L. However, hippuric acid in urine did not show any correlation with styrene in air. Urine mandelic acid excretion expected ratio showed a tendency to decrease according to obesity index and to increase with alcohol consumption.

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

본교 대학원 발전방안에 관한 연구

김종달,전도웅,전인철,조병권,박영규,오준석,나영일 龍仁大學校 1998 용인대학교 논문집 Vol.15 No.-

The purpose of this study was to suggest the developmental ideas for graduate school in Yougin University. For the methods of this study, the researches in administration of graduate students, personal researches in socialization of graduate school, and evaluation materials for graduate school were utilized. Additionally, the questionnaire survey about graduate students leas administered and analyzed and the ideas constructed from staff meeting for the development of graduate school were applied in preparing developmental plan. The results of this study are summarized as follow: 1. 1-2 years preparation - offers annual credits upto 30 - jop marketing upto 70% - advisor and student ratio 1 : 7 - professors' doctoral degree possession upto 90% - graduate school professors' research achievement upto 2 articles annually - research space per student upto 10m/2 3-4 years preparation - offers annual credits unto 33 - advisor and student ratio 1:4 - professors' doctoral degree possession upto 95% - graduate school professors' research achievement upto 2.5 articles annually 2. The educational goals of graduate school should be recognized among graduate students and the professionality of faculty members should be emphasized to teach graduate students in their relative major areas. The evaluation procedures for graduate class lecture and class administration in graduate school. 3. To reach unto lecture system, field study should be expanded and inter-credit system between Yongin universities and foreign university should be contracted. 4. Expanding of graduate school facilities should be cranked in to provine the optimal environment for the student education and schoolship should be widely offered to the students. 5. To promote the administrational development, the staff system should be changed from reverse hierarchy system to hierarchy system. Therfore, the number in lower administrational staffs should be larger than upper positions. And, the classification of authorities into department could be suggested for the efficacy of administration and management of graduate shool. 6. The specialization in each major should be emphasized. In the calc of graduate stool of physical education, the administration live should be unified in one body system. In the case of graduate school of business, one department of administration could be classified into management of sightseeing and tourism, management of distribution, tax accounting, then the specialty of the department can be forced. In the case of graduate school of art, the specialty can be forced by teaching highly performed skills and needed to switch from special graduate school to professional graduate school. The graduate school of health and school of environmental science are recommended to be built after all. Therefore, the connection between undergraduate and graduate curiculum can be established and open 2-3 more majors in the graduate school.

구강암 세포주의 이소위 누드마우스 종양 모델

김종현(Jong-Hyun Kim),황영선(Young-Sun Hwang),김현실(Hyun-Sil Kim),남웅(Woong Nam),차인호(In-Ho Cha) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.2

In order to make successful oral cancer treatment, we need to understand about tumor biology and effective chemotherapeutic agents. To achieve these studies, it is necessary to develope a proper in-vivo model. Therefore the author will make try to develop more improved animal model of more applicable in various method of cancer study. In this study, the author induced in-vivo tumorigenesis in nude mice by YD-10Bmod cell line used by YD-10B cell line originated from oral tongue squamous cell carcinoma and observed tumor formations and invasiveness of surrounding tissue, and found some results as follows : 1. The experimental group (YD-10Bmod, subcutaneous injection) produced tumors 13 out of 15 mice, while the control group produced none of 5 mice. 2. The inoculation of 1×106cells/mouse produced tumors 3 out of 5 mice and inoculation of 1×107cells/mouse, 2×107cells/mouse produced tumors in every 5 mice. 3. In the histopathologic studies, the inoculation of 1×106cells/mouse group showed the characteristic features of well-differentiated squamous cell carcinoma and demarcated expansile growth, while the inoculation of 1×107cells/mouse, 2×107cells/mouse group showed the expansile growth with partial central necrosis and invasive growth to surrounding fat & connective tissue. These findings suggest that atopic xenograft of YD-10Bmod cell line in nude mice has a improved productivity of tumors, produced tumors showed the characteristics feature of human tumor and invasive growth to surrounding tissue in histopathologic appearance. These atopic nude mouse model of tongue carcinoma might assist in studying oral cancer biology and effective choice of chemotherapeutic agents.

장애인 공동체에 대한 연구 : Camphill을 중심으로 Docused on Camphill

이인영,신용웅 한국장애인재활협회 2004 재활복지 Vol.8 No.2

본 연구는 지적능력에 결함을 가진 발달장애인의 부모 사후 자녀의 영속적인 삶의 질에 대한 관심에서부터 출발하여 그 대안으로 특수교육과 사회복지분야에서 실험적인 모델로 관심을 끌고 있는 '장애인공동체'를 주제로 하고 있다. 특히 전 세계 20여개국에 100여개로 활성화되고 있는 Camphill 중 아일랜드 더스게릭 캠프힐(Duffucarrig Camphill)에 단기자원봉사활동을 토대로 문헌자료를 보강하여 진행하였다. 먼저, 장애인공동체의 대표적 모델로 손꼽히는 캠프힐의 성격을 규명하기 위해 캠프힐의 철학과 가치, 설립배경 및 과정, 위치와 규모, 구성원, 재정과 운영을 분석해보았다. 분석결과 캠프힐은 자급자족적 요건만을 충족하지 못할 뿐 그 외 공동체생활의 요소라 할 수 있는 성원 공동의 가치와 덕목 실현, 지역을 기반으로 하는 공동생활, 인격적 친밀감, 운영규칙에 의한 자치 등을 발견할 수 있었다. 또한 참여, 접근, 존재, 생산, 소비 등 5가지의 Segal & Aviram(1978) 다차원적 접근으로 캠프힐 내 생활, 생산, 여가 및 문화, 소비, 건강 및 의료, 자기결정을 분석해본 결과, 단지 물리적 통합이 아닌 지역사회내의 참여부분에 강점을 갖는 것으로 분석되었다. 또한 가정이나 지역사회안에서 대인관계의 원활성, 타인으로부터의 존중감, 개인의 취향이나 개별성 보장 등의 영역에서도 삶의 질을 담보하고 있었다. 이상의 연구를 통해 장애인공동체로서 캠프힐은 첫째, 발달장애인의 영속적인 삶을 위한 하나의 대안적 모델로 둘째, 발달장애인의 사회통합 뿐 아니라 삶의 질을 중시하고 있으며 셋째, 발달장애인의 복지를 바라보는 관점을 삶의 질 측면으로 끌어올리고, 지속적 삶의 질을 위해서 어떠한 자원들이, 어떻게 결합되어야 하는지에 대한 모델이 된다는 점에서 큰 시사점을 던져준다 할 것이다. The theme of this study is on 'the Community of People with Disability' which attracts our attention to as an alternative measure for the permanent quality of lives of children with developmental disabilities after their parents' death, as well as an experimental model in the fields of special education and social welfare. Especially, this article deals with short-term voluntary activities in Duffucarrig Camphill in Ireland among hundred Camphills, which have been actively run in over twenty countries, including its literature study. First of all, in order to clarify the characteristics of Camphill, which is well-known for a representative model among communities of people with disabilities, the philosophy and values of Camphill, its foundation background and process, location and scale, its members, and its finance and management are analyzed. As a consequence of the analysis, we can find the components of community lives, such as the common values of members, their realization of virtues, community lives based on the locality, humane intimacy, autonomy by its management rules, etc., except not satisfying a self-sufficiency component. Also, after analyzing life, production, leisure and culture, consumption, health and medicine, self-determination, etc, in Camphill through multidimensional approaches which are composed of five elements, such as participation, approch, existence, production, and consumption by Segal & Aviram(1978), it has strength in community participation, not only physical intergration. Besides, the quality of their lives are assured in the areas of good interpersonal relations, respectfulness by others, the right of choice, individual taste, individuality, etc. Thus, Camphill, as a coummunity of people with disabilities, can be described as follows: First, it can be a alternative model for the permanent lives of people with developmental disabilities: Second, it focuses on the quality of their lives as well as the social integration of people with developmental disabilities: Third, it gives suggestions in that it raises perspectives on the welfare of people with developmental disabilities up to the dimension of the quality of their lives, and can be a model to show what kind of resources should exist, and how they should be combined in order to guarantee the continual quality of their lives.

비골 골절의 임상적 고찰

변웅래,여환호,양인석,김영균 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

Because of the prominence of the nose and its central location, it is the most frequently encountered fractures in the face. Yet reports about the nasal bone fractures are virtually rare in the oral and maxillofacial surgical literatures. This sis a retrospective study on 19 nasal bone fractures treated in Chosun university hospital Department of Oral & Maxillofacial Surgery from Jan. 1991 to Sep. 1993, under admission to our Dept. and the obtained results were as follows. 1. Of the 240 patients with facial bone fractures, 28 patients suffered nasal fractures(12%) and male to female ratio was 5.3:1. 2. The most frequent cause was traffic accidents(39%)m, the next fall down(36%), first blow(4%). 3. The age frequency was the highest in the fifth decade (32%). 4. Clinical classification of nasal fractures was simple fractures(74%), comminuted fractures(26%), and single fractures(37%), combined fractures(63%). 5. The most frequently combined site was msxilla(50%). 6. Treatments of nasal fractures were closed reduction(63%), open reduction(5%), and secondary rhinoplasty(32%). 7. The initial treatment time from accident was 1.7 days in single fractures, and 3.5 days in combined fractures, and the period of splint retained was about 8.2 days in single fracture, about 8.7 in combined fracture. 8. It was necessary to treat secondarily in delayed treatment, and all treatment methods showed relatively good prognosis. 9. Closed reduction was treated under local anesthesia, but open reduction & secondary rhinoplasty was treated under general anesthesia except 1 case. 10. The complications were disturbance of swellings 5 cases, eithetic problem 5 cases, epiphora 3 cases, abnormal sensation 6 cases in relation with other fractures.

탈회 염산농도가 동종골 이식 치유 과정에 미치는 영향에 관한 실험적 연구

엄인웅(In Woong Um),김은철(Eun Cheol Kim) 대한구강악안면외과학회 1993 대한구강악안면외과학회지 Vol.19 No.2

It is generally beleived that the demineralization process does not remove BMP, while the undemineralized bone contains this protein, the bone mineral seems to hinder its action so that the demineralization process make the bone inducing agents more available to the host tissue. The purpose of this study was to evaluate the influence of HC1 concentration o the healing process of 0.4N, 0.5N, and 0.6N HC1 demineralized allogenic bone graft respectively compared with the autografted bone. The results were as follows: 1. Inflammatory cell infiltration appeared at 2 weeks and disappeared at 4 weeks in all experimental groups, but especillay severe in 0.4N HC1 demineralized group at 2 weeks. 2. Neovascularization around graft material in 0.5 and 0.6N groups were more than in 0.4N HC1 group. 3. Osteoblastic activity in 0.5 and 0.6N groups were more than in0.4 HC1 group. 4. There were no significant differences of bone resorption among 0.4, 0.5 and k0.6N HC1 groups. 5. New bone formation and mineralization in 0.5 and 0.6N HC1 groups were more than in 0.4N group and were similar to autograft group.

출생후 흰쥐 망막에서 recoverin면역반응 원뿔두극세포의 분화와 GABA성 무축삭세포와의 연접형성 시기

박대균,김인범,천명훈,정진웅 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

원발성 위장관 임파종의 임상적 고찰

윤진우,한지숙,고윤웅,박인서,최응재,주승행,김태승 大韓血液學會 1983 大韓血液學會誌 Vol.18 No.2

남부 평야지 자운영재배답에서 논유형과 질소수준이 벼 생육 및 토양 화학성에 미치는 영향

윤봉기,김병호,이인,최형국,박인진,김용웅 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

녹비작물로서 자운영을 재배한 논에 벼를 재배할 때 질소비료의 절감기준을 구명하고자 평야지인 전남 나주의 보통답과 사질답에서 관행, 자운영+질소 70%, 자운영+질소 50%, 자운영+질소 30%, 자운영+무질소의 5처리로 1999년부터 2001년까지 3년동안 시험한 결과는 다음과 같다. 1. 자운영의 개화성기 생육은 초장 43.6~43.9㎝, 생초중 21.79~24.99Mg ha^(-1)이였으며 무기성분 함량은 T-N 2.40~2.64%, P₂O_(5) 0.42~0.46%, K₂O 1.83~2.01%였다. 자운영의 생육은 보통답이 사질답에 비하여 생초중이 많았고 T-N, T-C, P₂O_(5), CaO, MgO의 함량도 높았다. 2. 토양중 NH₄-N함량의 경시적 변화는 이앙후 7일에 20.7~47.4㎎ kg^(-1)으로 점차 감소하였는데 처리에 따라서는 자운영+질소 70%, 자운영+질소 50% 시용구에서 높았다. 3. 벼의 생육은 사질답이 보통답에 비하여 초장 및 간장이 크고 ㎡당경수가 많았고 처리에 따라서는 자운영+질소 50%, 자운영+질소 30%시용구에서 생육이 좋았다. 식물체중의 T-N, P₂O_(5), K₂O함량도 자운영+질소 70%, 자운영+질소 50%시용구에서 많은 편이었다. 4. 시험후 토양의 화학성은 관행에 비하여 보통답의 자운영 시용구에서 OM, CEC, Ava, P₂O_(5)함량이 증가하였고 사질답에서는 CEC가 증가하였다. 5. 벼의 수량은 관행(보통답 5.15Mg ha^(-1), 사질답 5.16Mg ha^(-1))에 비하여 보통답에서는 자운영+질소 70%, 50%, 30%시용구에서 각각 3, 6, 4% 증수되었고, 사질답에서는 자운영+질소 70%, 50%시용구에서 각각 3, 5%증수되었다. 6. 남부 평야지에서 자운영을 재배하여 생초 20Mg ha^(-1)를 녹비로 투입하면 벼를 재배시 질수시용 적정량을 보통답에서는 관행시비량의 30%, 사질답에서는 관행시비량의 50%이였다. This study was conducted to find out an amount of N fertilizer for rice culture followed after milk vetch(MV) cultivated at normal and sandy paddy field in Naju which lies to southern plain region from 1999 to 2001. The amounts of N fertilizer were 110(normal paddy) and 130(sandy paddy)㎏ ha^(-1) at control and 0, 30, 50, 70% of control. At flowering stage of MV, Plant height was 43.6~43.9㎝ and fresh weight was more than that of sandy paddy field. Also, the contents of T-N, T-C, P₂O_(5), CaO, MgO in MV plant cultivated normal paddy field were higher than those of sandy paddy field. Content of NH₄-N in soil was 20.7~47.4㎎ ㎏^(-1) at 7days after transplanting and 6.1~8.1㎎ ㎏^(-1) at 70days after transplanting of rice plant. Content of NH₄-N in soil was high at the treatments of MV+N50% and MV+N70%. Rice plant height, culm length and the number of tiller per ㎡ in sandy paddy field were higher than those of normal paddy field. Growth of rice plant in MV+N70% and MV+N50% was higher than that in others. And the content of T-N, P₂O_(5) and K₂O of rice plant in MV+N70% and MV+N50% were higher than those in others. Chemical properties of soil after experiment were pH 5.1~5.9, OM 15.6~20.9g ㎏^(-1). CEC 10.1~13.8cmol ㎏^(-1) and Ava. P₂O_(5) 152~244㎎ ㎏^(-1). The contents of OM. CEC and Ava. P₂O_(5) in soil applied MV at normal paddy field were higher than those of the control, and the content of CEC in soil applied MV at flowering stage, rice yields of MV+N70%, 50%, MV+N30% were increased 3, 6, 4% more than that of control (5.15Mg ha^(-1)) at normal paddy field. Rice yields of MV+N70%, MV+N50% were increased 3, 5% higher than that of control(5.16Mg ha^(-1)) at sandy paddy field. Nitrogen application rate of normal paddy field was 30%, and sandy paddy field was 50% of conventional fertilization rate when 20Mg ha^(-1) MV cultivated paddy field was inputed for rice culture in the southern plains.