A Novel Small-Molecule Inhibitor Targeting the IL-6 Receptor β Subunit, Glycoprotein 130

Hong, Soon-Sun,Choi, Jung Ho,Lee, Sung Yoon,Park, Yeon-Hwa,Park, Kyung-Yeon,Lee, Joo Young,Kim, Juyoung,Gajulapati, Veeraswamy,Goo, Ja-Il,Singh, Sarbjit,Lee, Kyeong,Kim, Young-Kook,Im, So Hee,Ahn, Sun The American Association of Immunologists, Inc. 2015 JOURNAL OF IMMUNOLOGY Vol.195 No.1

<P>IL-6 is a major causative factor of inflammatory disease. Although IL-6 and its signaling pathways are promising targets, orally available small-molecule drugs specific for IL-6 have not been developed. To discover IL-6 antagonists, we screened our in-house chemical library and identified-LMT-28, a novel synthetic compound, as a candidate IL-6 blocker. The activity, mechanism of action, and direct molecular target of LMT-28 were investigated. A reporter gene assay showed that LMT-28 suppressed activation of STAT3 induced by IL-6, but not activation induced by leukemia inhibitory factor. In addition, LMT-28 downregulated IL-6-stimulated phosphorylation of STAT3, gp130, and JAK2 protein and substantially inhibited IL-6-dependent TF-1 cell proliferation. LMT-28 antagonized IL-6-induced TNF-alpha production in vivo. In pathologic models, oral administration of LMT-28 alleviated collagen-induced arthritis and acute pancreatitis in mice. Based on the observation of upstream IL-6 signal inhibition by LMT-28, we hypothesized IL-6, IL-6R alpha, or gp130 to be putative molecular targets. We subsequently demonstrated direct interaction of LMT-28 with gp130 and specific reduction of IL-6/IL-6R alpha complex binding to gp130 in the presence of LMT-28, which was measured by surface plasmon resonance analysis. Taken together, our data suggest that LMT-28 is a novel synthetic IL-6 inhibitor that functions through direct binding to gp130.</P>

OMC-2010 추출물이 마우스의 비장세포 cytokine 생성에 미치는 영향

배기상 ( Gi Sang Bae ),박경철 ( Kyoung Chel Park ),최선복 ( Sun Bok Choi ),조일주 ( Il Joo Jo ),서상완 ( Sang Wan Seo ),김종진 ( Jong Jin Kim ),신용국 ( Yong Kook Shin ),김민선 ( Min Sun Kim ),박규환 ( Kyu Hwan Park ),김현식 ( Hyu 대한본초학회 2012 大韓本草學會誌 Vol.27 No.5

Objective : This study was performed to estimate the effects of OMC-2010 extract on cytokine production in mouse spleen cells. Methods : Mouse spleen cells were pre-treated with ethanol and water extract of OMC-2010 for 1 h, then stimulated with lipopolysaccharide (LPS, 1 μg/ml) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokines. Results : OMC-2010 ethanol extract significantly inhibited the LPS-induced interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and IL-5 mRNA expressions, but not shown such changes in IL-6, IL-4, IL-13. OMC-2010 water extract significantly inhibited the LPS-induced TNF-alpha, and IL-5 mRNA expressions, but not shown such changes in IL-1beta, IL-6, IL-4, IL-13. Conclusions : Theses results could suggest that both ethanol and water OMC-2010 extract could inhibit the TNF-alpha and IL-5 mRNA expression.

오대산국립공원 내 뱀류 로드킬 분포현황 및 발생예측 모델링

김석범,박일국,박대식,Kim, Seok-Bum,Park, Il-Kook,Park, Daesik 한국환경생태학회 2022 한국환경생태학회지 Vol.36 No.5

오대산국립공원 내 뱀류 로드킬의 발생 경향 파악 및 예방을 위하여 2006-2017년 사이 공원 내에서 발생한 뱀류 로드킬 자료를 확보 및 분석하였고, 잠재적 발생지 예측을 위하여 종분포모델을 제작하였다. 연구기간 동안 뱀류 로드킬은 600m 대의 양쪽 환경이 산림-수계인 도로에서 가장 많이 발생하였다. 모델링 결과에서 뱀류 로드킬 발생 가능성은 고도 700m 이하의 하천과의 거리가 25m 부근인 완만한 경사의 도로의 로드킬 발생확률이 높게 나타났다. 국립공원 내 주요 로드킬발생 예측지역은 국도 6호선 도로 위 공원 남쪽 경계로부터 약 2.2km 지역과 약 11.7km 지역이, 지방도 446호선 도로 위 공원 남쪽 경계로부터 약 3.44km 지역이었다. 본 연구결과는 해발고도 700m 이하 수계와 인접한 도로 주변에 우선적으로 대체 일광욕 장소, 생태통로 및 도로의 유입을 막는 울타리의 설치가 산림에서 뱀류 로드킬을 줄이는 효과적인 방안이 될 것을 제시한다. In this study, we collected snake roadkill data from 2006 to 2017 and developed a species distribution model to identify the pattern of snake roadkill and predict the potential hotspot of snake roadkill in the Odaesan National Park of South Korea. During the study period, snake roadkills occurred most frequently on the road, which passes through between forest and stream at an altitude of about 600 m. The modeling result showed that the occurrence probability of snake roadkill was high on a road with a gentle slope at a distance of 25 m from the stream and an altitude of 600 m. The most susceptible regions for snake roadkill in the Odaesan National Park were located on National Route 6, about 2.2 km and 11.7 km away from the southern border of the park, and on Local Road 446, 3.44 km away from the southern border of the park. The results of this study suggest that providing alternative basking places and eco-corridors and installing protection fences that block the inflow of snakes into roads, preferentially around roads and streams at an altitude lower than 700 m would be an effective way of reducing snake roadkill in the Odaesan National Park.

Interleukin-2와 결핵균 30 kDa 항원이 구개편도 및 말초혈액 T 세포 증식에 미치는 상승효과

박정규,박찬권,조은경,김화중,백태현,고필준,김병국,남부현,나기상,박찬일 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

Widespread use of BCG has not controlled tuberculosis, and more effective vaccines are clearly needed. Although chemotherapy will remain the mainstay of antituberculosis treatment, the use of adjunctive immunotherapeutic modalitites is attractive, particularly in persons with drug-resistant tuberculosis. Administration of IL-2 or IFN-γto tuberculosis patients enhance bacillary elimination. Cell-mediated immunity is the critical protective immune response in tuberculosis. Mycobacterial antigens are recognized by T cells and that elicit production of protective cytokines are potentially important vaccine antigens. The 30 kDa antigen is secreted in large quantities by growing mycobacteria. That antigen elicits greater proliferation in lymphocytes from healthy tuberculin reactors than healthy tuberculin nonreactors. In this study, the T lymphocyte proliferative responses to 30 kDa antigen from Mycobactrium tuberculosis H37Rv were examined by using tonsilar and peripheral blood lymphocytes from PPD(+) and PPD(-) tonsilectomized persons. When cultured with 30 kD antigen, tonsilar mononuclear leukocytes and T cells of PPD(+) demonstrated more ^3H-thymidine incorporation than PPD(-) persons (stimulation index was 2.5 and 1.9, 0.8 and 1.0, repectively). Peripheral blood mononuclear cells (PBMC) and peripheral blood T lymphocytes were shown the similar responses to this antigen. The combination of IL-2 and 30 kDa antigen elicited a significant proliferative responsiveness in tonsilar mononuclear leukocytes and T cells of PPD(+) persons (SI was 20 and 14.1). PBMC and peripheral blood T cells of PPD(+) persons were also shown a significant responsiveness, but PPD(-) persons did not show. These results demonstrate that the 30 kDa antigen and IL-2 have a synergistic stimulatory property in mycobacteria sensitizing lymphocytes.

분석결과 검증 및 신뢰성 확보를 위한 실험실간 협력 실험

박혜진,고광용,한국탁,김일중,이용재,김성헌,이규승 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.2

The residual study of pesticide has been used in various areas, such as food safety, environmental protection, establishment of tolerance, and explaining the pathway and reaction mode of pesticides, and its importance was expected to increase further more. The aspect of food safety, the pesticide residue survey have been practiced at many organizations, but there were no verification of analytical results at present. In this experiment, we focused on instrumental stability, including response of each instrument and the recovery ratio of each organization's method. As samples for this experiment, we prepared cucumber and sesame, and chose 4 pesticides (bifenthrin, chlorpyrifos, diazinon, and ethoprophos), which were mostly detected from pesticide residue survey and widely used for each crop. The standard deviation of peak areas in the chromatogram of each pesticide were under 1.212 %, so it showed that most instruments were stable. The relationship of recovery ratio of each organization were over 0.996 for every pesticide and each organization. Finally, the analytical results for pesticide residue from each participated organization were not statically significant and we could put confidence in the result from each organization.

Zns AC박막형 EL소자의 물성에 관한 연구

김영국,김일홍,박성훈 경남대학교 환경문제연구소 1989 환경연구 Vol.11 No.-

A ZnS : SmF₃, ZnS : T_(b)F₃ do thin film electroluminescence devious has been fabricated by electron beam evaporation method. After the evaporation, the brat treatment has been made at 450°C for 1 hour in high vacuum. The brightness-voltage characteristic for do operation has been measured. This electroluminescence devices shows red, green emission with maximum intensity at wavelength 710㎚, 580㎚.

原乳의 세균수 等級制 실시에 따른 미생물학적 품질개선

姜國熙,朴信仁,鄭忠一 성균관대학교 생명과학자원연구소 1994 生命資源科學硏究 Vol.1 No.2

Raw milk grading system based on the bacterial count was started in Korea from June 1, 1993. The production ratio of first class which has 100,000/ml below in the bacterial counts of raw milk increased from 21% to 60% during 6 months. But, the ratio of the first class which has 250,000/ml below in the somatic cells did not change and became to be worse. In somatic cells the ratio of the first class milk was 42% in June 1993 and after 6 months it was decreased to 30%, Because current grade system based on the somatic cells give an penalty only to the off-grade milk which contains more of 750,000cell/ml. The system should be changed to pay different money according to the different classes of somatic cells in the raw milk.

복합 산화법과 MEMS 기술을 이용한 RF용 두꺼운 산화막 에어 브리지 및 공면 전송선의 제조

김국진,박정용,이동인,이봉희,배영호,이종현,박세일 한국센서학회 2002 센서학회지 Vol.11 No.3

본 논문에서는 양극반응과 복합 산화법( H_2O/O_2 분위기에서 500℃, 1시간 열산화와 1050℃, 2분간 RTO(Rapid Thermal Oxidation) 공정)을 이용한 두꺼운 OPSL(Oxidized Porous Silicon Layer)을 형성하여 이를 마이크로머시닝 기술을 이용함으로써 10 ㎛두께의 OPS(Oxidized Porous Silicon) 에어 브리지를 제조하고, 그 위에 전송선로를 형성하여 그 RF 특성을 조사하였다. OPS 에어 브리지 위에 형성된 CPW(Coplanar Waveguide)의 손실이 OPSL 위에 형성된 전송선의 삽입손실보다 약 2 dB 정도 적은 것을 보여주었으며, 반사손실은 OPSL 위에 형성된 전송선의 반사손실보다 적으며 약 -20 dB를 넘지 않고 있다. 본 연구에서 개발한 산화된 다공질실리콘 멤브레인 및 에어 브리지 구조는 CMOS 공정 후에 사용 가능하며, 초고주파 회로 설계시 편리성과 유용성을 제시하고 있다. This paper proposes a 10 ㎛ thick oxide air-bridge structure which can be used as a substrate for RF circuits. The structure was fabricated by anodic reaction, complex oxidation and micromachining technology using TMAH etching. High quality films were obtained by combining low temperature thermal oxidation (500 ℃,1 hr at H_2O/O_2) and rapid thermal oxidation (RTO) process (1050 ℃, 2min). This structure is mechanically stable because of thick oxide layer up to 10 ㎛ and is expected to solve the problem of high dielectric loss of silicon substrate in RF region. The properties of the transmission line formed on the oxidized porous silicon (OPS) air-bridge were investigated and compared with those of the transmission line formed on the OPS layers. The insertion loss of coplanar waveguide (CPW) on OPS air-bridge was (about 2 dB) lower than that of CPW on OPS layers. Also, the return loss of CPW on OPS air-bridge was less than about - 20 dB at measured frequency region for 2.2 mm. Therefore, this technology is very promising for extending the use of CMOS circuitry to higher RF frequencies.

폐혈관상이 유지된 선천성 낭성 선종양 기형

배일헌,한기석,차상훈,김성진,박길선,이건국,홍장수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

선천성 낭성 선종양 기형은 태생 16주 이후 말단세기관지의 형성이 중단되어 미성숙기관지들과 중배엽성분들이 과도하게 증식하여 발생하는 드문 선천성 폐질환이다. CT소견은 낭종의 크기 숫자, 내용물 등에 따라 다양하나, 병변내에 정상적인 폐혈관이 보인 예는 전세계적으로 보고된 적이 없었다. 저자들은 역동적 흉부 CT상 균일한 저음영의 병변내에 정상 폐혈관상이 유지된 선천성 낭성 선종양 기형 1예를 경험하였기에 보고한다. Congenital cystic adenomatoid malformation(CCAM) is a rare pulmonary disease that is thought to be developed by abnormal proliferation of immature alveoli and other mesenchymal component from the abrupt halting of the terminal alveoli formation after 16 weeks of gestation. On CT scan, there are no consistencies regarding to size, numbers, and its contents but no cases were reported in past showing normal pulmonary vascularities. We reports a case of CCAM that showed normal pulmonary vascularities within the hypodense lesions on a dynamic chest CT

형상기억복합재료의 제조공정 및 신뢰성 평가

이진경,박영철,이규창,최일국,이준현 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.6

형상기억복합재료는 복합재료내에 압축잔류응력을 발생시킴으로써 재료의 인장강도를 증가시키는데 사용되어지고 있다. 본 연구에서는 형상기억복합재료를 제조하기 위하여 TiNi 강화재와 A16061 기지재를 사요하였으며 핫프레스 방법에 의해 TiNi/A16061 형상기억복합재료를 제조하였다. 그러나 핫 프레스 방법에 의해 제조된 형상기억복합재료는 하중을 받을시 강화재와 기지재 사이에 계면손상현상이 발생하였으며 이를 위하여 냉간압연을 실시하였다. 냉간압연을 받은 시험편은 냉간압연을 받지 않은 시험편에 비하여 인장강도가 현저히 증가하였다. 또한 본 연구에서는 고온에서 형상기억복합재료의 미시적 손상을 평가하기 위하여 음향방출기법을 이용하였다. Shape memory alloy has been used to improve the tensile strength of composite by the occurrence of compressive residual stress in matrix using its shape memory effect. In order to fabricate shape memory alloy composite, TiNi alloy and Al6061 were used as reinforcing material and matrix, respectively. In this study, TiNi/Al6061 shape memory alloy composite was made by using hot press method. However, the specimen fabricated by this method had the bonding problem at the boundary between TiNi fiber and Al matrix when the load was applied to it. A cold rolling was imposed to the specimen to improve the bonding effect. It was found that tensile strength of specimen subjected to cold rolling was more increased than that of specimen which did not underwent cold rolling. In addition, acoustic emission technique was used to quantify the microscopic damage behavior of cold rolled TiNi/Al6061 shape memory alloy composite at high temperature.