다환방향족 탄화수소에 오염된 토양에서 분리한 Pseudomonas sp. KK1의 생리학적 특성

윤병준,강형일,오덕철 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-

Strain KK1 was isolated from soil contaminated with polycyclic aromatic hydrocarbons and able to mineralize anthracene, naphthalene, and phenanthrene. In this study, strain KK1 was tested to evaluate carbazole-degrading potential using radiorespirometry. When KK1 was pre-grown on phenanthrene the cells were able to mineralize carbazole much more rapidly, suggesting a possible close linkage between the pathways for catabolism of carbazole and phenanthrene. Such an increase in carbazole degradation was not appreciable when KK1 was pre-grown on naphthalene. Strain KK1 was identified as the genus Pseudomonas with over 90 % confidence based on BIOLOG system and FAME analysis. Analysis of PLFAs extracted from kk1 cells on carbazole medium revealed that lipids 10:0 3OH, 17:0 cyclo, and 18:0 were representatives produced or significantly increased in response to carbazole. Tests for antibiotics provided the data that strain KK1 was resistant to antibiotic ampicillin, but susceptibile to chloramphenicol, gentamycin, kanamycin, streptomycin, and tetracycline. Strain KK1 demonstrated strong resistance to most heavy metals such as Ba, Cd, Fe, Hg, Pb used in this study.

운동요법과 침술요법 병행에 의한 비만치료의 효과

윤형권,이철원 公州大學校스포츠科學硏究所 1998 스포츠科學硏究所論文集 Vol.- No.12

Until recently, 27.3 percent of Koreans are overweight and 3.3 percent are obese which is equal to about a third of the whole nation. Obesity is even becoming a social problem surpassing personal matter. Obesity can be induced to diabetes mellitus, hyperten, artherosclerosis, hyperlipoidemia, heart and C.V.A disease, etc Therefore this study is aimed at presenting evidence for an economical and efficent therapy with proving the effect of acupunture and exercisc in obesity. This three-month research project had done in Nonsan for July 1-Sept.30, 1997. The housewives who were studied had more than 25 percent of body fat, between twenty-five and fifty years old. Each group consisted of ten housewives. One group of subjects embarked on both acupunture and exercise. The other group was given just by acupunture. Aerobics, at 60 percent of HRmax level, were regularly and steadily performed for an hour every day, four or five times a week as an exercise therapy. Acupunture therapy was conducted two or three or three times per week. The conclusion measured and anslsysized the change of weight and body fat rate of each group follows: 1. The change of Weight 1) The group with exercise and acupunture together showed the change of weight from 54.5±4.1kg 2 weeks later to 50.8±3.4kg 12 weeks later. The reduced average weight was 3.7kg with a statistical significance of p〈0.01. 2) The other group with acupunture alone revealed the change of wieght from 55.6±3.9kg to 53.8±4.0kg 12 weeks later. The reduced average weight was 1.8kg with a significance of p〈0.01. 3) The group with exercise and acupunture together did not show the reduced average weight 0.8kg until 2 weeks after the start of experiment. While the other group with acupunture alone showed the reduced average weight 0.7kg 4 weeks later with al significance of p〈0.01. The group with exercise an acupunture together lost weight more quickly than the other group with acupunture alone. 2. The change of body fat rate 1) The group with exercise and acupunture together showed the change of body fat rate from 29.2 ± 3.4% to 4.7 ± 3.0% 12 weeks later. The reduced average amount was 4.5% with a significance of p〈0.01. 2) The other group with acupunture alone revealed the change of body fat rate from 29.2 ± 2.5% to 27.0 ± 2.5% 12 weeks later. The reduced average amount was 2.2% with a significance of p〈0.01. 3) The group with exercise and acupunture the reduced average body fat was 1.1% 2 weeks later with a significance of p〈0.01. Changed much faster than the other group with acupunture alone showed the reduced average amount 1.0% with a significance of p〈0.01. In Conclusion, exercise and acupunture together is more beneficial and helps reduce weight and body fat rate more than acupunture alone. The tact that acupunture helps the reduced weight 1.8kg, the reduced body fat rate 2.2% respectivly with a significance of p〈0.01, p〈0.01 proves the effect of acupunture in obesity cure. I,as based on above conclusion, should suggest that prevention is much more important than cure in obesity. It is required that regular and proper exercise at greater than fixed rate and accentuating not only the control of Weight but control of body fat rate to cure, or prevent obesity. We need various studies on cure method of obesity. A study is lively demanded by applying medicine under the eye lately to a physical education field.

항정신병 약물치료하에서 주관적 안녕감을 평가하는 한국형 척도의 개발

윤진상,국승희,이형영,이철,백인호 대한신경정신의학회 2000 신경정신의학 Vol.39 No.6

연구목적 : 본 연구의 목적은 1994년 Naber 등이 개발한 38문항의 '항정신병 약물치료하에서 주관적 안녕감을 평가하는 자가평정척도(a self-rating scale to measure subjective well-being under neuroleptic tre-atment : SWN)'를 문항수를 줄이고 문항내용을 수정하여 한국형으로 개발하고자 하였다 방 법 : 204명의 정신분열병 환자들에게 22문항의 KmSWN과 General Health Questionnaire/Quality of Life-12(GHQ/QL-12)를 실시하였고, 사회인구학적 및 임상적 특징을 조사하였다. 자료분석에는 척도에 대한 반응과 정보가 신뢰로운 176명의 자료를 포함시켰다. 첫째, KmSWN의 신뢰도와 타당도를 평가하였다. 둘째, KmSWN총점과 사회인구학적 및 임상적 특징의 관계를 조사하였다. 끝으로 KmSWN의 총점을 예측하는 변인들을 알아보기 위해 KmSWN총점과 유의한 상관이 있는 변인들을 독립변인으로 하고 KmSWN총점을 종속변인으로 하여 단계적 중다회귀분석을 실시하였다. 결 과 : 22문항의 KmSWN중 세문항은 내적 일치도가 낮아서 추후분석에서 제외되었다. 19문항의 KmSWN(KmSWN-19)은 반분신뢰도(.91), 문항간 상관의 평균(.38), 내적 일치도(Cronbach alpha .92)가 높았다. 요인분석에서는 '불만족', '신체 및 정신기능', '정서조절 및 자기통제'의 3개요인이 추출되었다. KsSWN-19총점과 GHQ/QL-12총점간의 상관은 -.83으로 공존타당도가 높았다. 사회인구학적 및 임상적 특징중 연령, 용돈, 사회경제적 수준, 과거 입원횟수, 투약에 대한 순응 투약에 대한 만족도는 KmSWN-19 총점과 정적 상관이 있었던 반면, 질병의 심도, 파킨슨 증상의 심도, 지연성 운동장애의 심도, 투약횟수 및 투약을 빠뜨린 횟수는 KmSWN-19 총점과 부적 상관이 있었다, 단계적 중다회귀분석결과, KmSWN-19총점을 유의하게 예측하는 변인은 투약의 만족도, 투약을 빠뜨린 횟수 및 용돈이었으며, 변량의 24%를 설명하였다. 결 론 : KmSWN-19는 정신분열병 외래환자의 주관적 안녕감을 측정하는 자기평정척도로서 신뢰롭고, 타당하며 실용적임이 확인되었다. 아울러 KmSWN-19는 항정신병 약물과 관련한 주관적 안녕감에만 특이한 척도가 아니라, 척도에 응답할 수 있는 모든 정신분열병 환자나 기타 정신증 환자들에게 적용할수 있는 일반적 척도임이 시사되었다. 한편, KmSWN-19총점에 대해 독립변인들의 설명변량이 크지 않는 점을 주관적 안녕감 혹은 삶의 질의 평가에서 개인의 가치나 신념, 성격특성 같은 개인내적 특성을 고려해야 할 필요성이 있음을 시사한다. Objective : The purpose of this study was to develop the Korean modification of SWN(KmSWN), based on the original 38-item SWN(a self-rating scale to measure subjective well-being under neuroleptic treatment)by Naber et. al. In 1994, but containing the reduced number of items and the modified content. Methods: The KmSWN with 22 items and General Health Questionnaire/Quality of Life-12(GHQ/QL-12) were administered to 204 out-patients with schizophrenia. Also, their socio-demographic and clinical characteristics were investigated. Data analyses were conducted based on 176 patients whose responses to the scale, as well as whose related informations, were reliable. First, the reliability and validity of KmSWN were assessed comprehensively. Second, the relationships between the total score of KmSWN and the sociodemographic and clinical characteristics were examined. Finally, in order to identify the predictable variables on the total score of KmSWN, the stepwise multiple regression analysis was conducted, where the inde-pendent variables were already shown significant correlations with the total score of KmSWN. Results : Three of 22 items of KmSWN were excluded from the following analysis due to their poor internal consistency. KmSWN comprised with 19 items(KmSWN-19) showed high levels of spilt-half reliability(.91), mean of inter-item correlations(.38) and internal consistency (Cronbach alpha .92). Factor analysis of the KmSWN-19 revealed that a three-factor solution accounted for 100% of total explained variance. The three factors were interpreted as 'diss-atisfaction','physical and mental functioning', 'emotional regulation and self-control', The concurrent validity of KmSWN-19 with GHQ/QL-12 was very high(-.83). Of the socio-demographic and clinical characteristics, age, disposable money, socioeconomic level, number of previous hospitalizations, compliance with medication and satisfaction with medication were positively correlated with the total score of KmSWN-19, while severity of illness, severity of parkinsonism, severity of dyskinesia, administration frequency of medication and omission number of medication were negatively correlated. However, the total score of KmSWN-19 was not correlated with medication, the omission number of medication and the disposable money were significantly contributed to the total score of KmSWN-19, and they explained 24% of the variance. Conclusions : The above findings indicated that KmSWN-19 was a reliable, valid and pra-ctical self-rating scale to measure subjective well-being in out-patients with schizophrenia. Moreover, KmSWN-19 seemed to be a general scale applicable to all the schizophrenic or other psychotic patients if they were able to complete it, rather a specific scale only for the patients undergoing the neuroleptic treatment. Based on the results of the predictable variables on the total score of KmSWN-19, one may argue that intra-individual characteristics such as value, belief and personality traits, although they were not evaluated in this study, should be considered in assessing subjective well-being or the quality of life.

SiH_4 기체 방전 파라미터 해석

이형윤,하성철,유회영,김상남,임상원,문기석 동국대학교 산업기술연구원 1997 산업기술논문집 Vol.9 No.-

This paper describes the discharge parameters in SiH_4 gas calculated for the range of E/N values from 0.5~300(Td) by the Monte Calro simulation and Boltzmann equation method using a set of electron collision cross sections determined by the authors and the values of the discharge parameters are obtained for the TOF method. The theoretical results of the discharge parameter such as the electron drift velocity, characteristic energy agree with the experimental values for the range of E/N. The electron energy distributions function were analyzed in monosilane at the E/N : 30, 50(Td) for a case of equilibrium region in the mean electron energy. The validity of the results obtained has been confirmed by the TOF method.

개인방사선피폭선량평가

이형섭,김장렬,윤석철,이상윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.2

최근, 개인방사선피폭선량평가에 관한 기술기준이 과학기술처에 의해 고시됨에 따라 국내에서도 개인선량계를 이용한 체외피폭선량평가결과의 신뢰성 문제가 크게 대두되고 있다. 한국원자력연구소에서는 이러한 구내의 상황을 인식하고 자체적인 신뢰성 확보를 위해 미국의 Oak Ridge국립연구소의 주관하에 개인피폭선량의 평가에 관한 국제상호비교검증시험(Personnel Dosimetry Intercomparison Study ; PDIS) 을 수행하였다. 비교 검증시험에는 한국원자력연구소에서 사용하고 있는 Teledyne PB-3 열형광선량계가 사용되였으며 선량게산 알고리즘은 Teledyne PB-3 version1.5-1989를 사용하였다. 본 연구에서는 지금까지 실시된 PDIS의 결과를 요약하고 현재 PB-3 시스템의 개인선량평가성능에 대해 고찰하였으며, 선량평가절차에 대한 문제점 도출을 통하여 직업적 방사선피폭선량 평가능력의 향상을 위한 방안을 제시하였다. Recently, the Ministry of Science and Technology issued a Ministerial Ordinance (N0 1992-15) about the technical criteria on personnel radiation dosimetry. In today's climate, it is important to demonstrate and document that the processor's systems and services to others meet national standards of quality. The purpose of this study is to verify the performance of the Teledyne PB-3 personnel dosimetry system that is generally used in Korea Atomic Energy Research Institute (KAERI) by intercomparison with Oak Ridge National Laboratory. The KAERI has been participated in this personnel dosimetry intercomparison study(PDIS) program since 1991 and it could be possible to test and calibrate personnel monitoring system. This report presents a summary and analysis of by about 50 does equivalent measurements reported for PDIS-16 through 18 (1991-1993) with emphasis on neutron does equivalent sensitivity, accuracy and methods to improve personnel dosimetry performance are also discussed.

直射日光의 水面反射遮蔽를 위한 窓計劃에 관한 硏究 : 大邱 地方을 中心으로

韓允昊,金相徹,尹亨洙 嶺南大學校 環境問題硏究所 1990 環境硏究 Vol.9 No.2

窓의 前面에 넓은 수면이 전개되어 있는 경우에 直射日光 뿐만이 아니라 水面에 의한 反射光이 동시에 室內로 사입되어 실내의 熱環境을 열악하게 만들며 수면으로 인한 Glare 현상으로 眺望機能이 극히 악화된다. 따라서 本 稿에서는 建築的 手法을 통한 효율적 조절방법으로서, 遮陽과 발코니를 이용하여 眺望의 기능을 최대한으로 확보하면서 室內過熱의 원인이 되는 直射日光 및 反射光의 遮蔽 방법을 연구하였다. 그 결과를 요약하면 다음과 같다. 1. 水面 反射光은 넓은 발코니를 이용하여 日照環境이 나빠지기 쉬운 向에서도 眺望機能의 큰 손상없이 효율적으로 차폐가 가능하다. 2. 連續窓 계획시 遮陽計劃은 각 陰影角에 변화를 주어 格子遮陽裝置로 개량할 수 있다. 3. 日射遮蔽를 목적으로 계획된 遮陽裝置가 그 차양장치의 陰影마스크 만큼의 기간동안에는 窓을 통한 Glare 현상을 防止할 수 있다. In a case that there is broad water surface in front of windows, not only direct sun beams but also reflected sunbeams get into interior spaces. So indoor thermal environment is ready to spoiled and view from interior spaces through windows could also be impaired because of glare caused by reflected sun beams from water surface. In this paper, as an effective passive control, a method to provide a large solid angle for view through windows and to prevent direct and reflected sun beams with shading devices and balconies is studied. Results are as follows. 1. Planning broad balconies, reflected sun beams from water surface could be prevented effectively without damaging the view through windows. 2. In cases of planning shading devices for horizontally linear windows, eggcrate shading devices could be made by altering shading mask of horizontal and, or vertical shading devices. 3. Although shading devices are designed for covering direct and reflected sun beams from water surface, they could also prevent the glace from sky and water surface during the period of shading masks of shading devices.

Moon, Yoon-Hee

문윤희,권영창,정인철,이형걸 慶星大學校 1995 論文集 Vol.16 No.2

Frozen beef loin imported from Australia was stored as 2℃ after thawing, then evaluate to improvement of raw meat aroma and palatability of cooked meat by sensory evaluation. To improvement of raw meat aroma and palatability of cooked meat of thawed beef were confirmed for the reason of conditioning after thaw as a result of myofibrillar fragmentation index(MFI) and Mg-ATPase activity of myofibril. Sensory evaluation of raw meat aroma and tenderness on frozen imported beef loin stored at 2℃ after thawing had good in all during cold storage, MFI and Mg-ATPase activity increased, therefore frozen beef loin could recognized to conditioning during stored at 2℃ after thawing. Cold storage after thawing of frozen imported beef loin can improve its cooked aroma and tenderness in case that insufficient conditioning is the reason for its low palatability, and the improvement of palatability on the cooked meat appeared to be greater in aroma than taste. In case of amino acid composition, aspartic acid, threonine, glutamic acid and alanine of imported beef loin stored 7 days after thawing were showed significantly(p<0.05) higher than that stored 0 day after thawing, total amino acid composition of imported beef loin stored 0,3 and 7 days after thawing were 148.40, 171. 06 and 188.99mg/g, respectively.

인체의 수동적 전기특성 계측법 개발

이현철,윤재현,박형준 원광대학교 공업기술개발연구소 2003 工業技術開發硏究誌 Vol.23 No.-

In this study, a system for the method measuring of passive electrical properties in the human body was developed. The system was composed of the stimulating part for input with voltage(about 2[㎷]) and of the measuring part for measurement of electrical properties in human body. As a result of this experiment, the frequency characteristic of each subject represent that the electrical properties goes up in spite of a constant stimulate-voltage according to frequency (l[㎐]-50[㎑]) increment. Namely, the amplitude of stimulate-signal was not reflected but frequency was reflected on the measured results. This result be estimated that the proposed system is able to measure the passive electrical properties of human body. Also, it would be estimated neural signal propagation directions by using the method developed in this study was measured passive electrical properties in each part of body.

제주연안 갯녹음(백화) 지역의 해수에 분포하는 세균군의 분자생물학적 분석

강봉조,김미란,윤병준,이동헌,오덕철,강형일 濟州大學校 基礎科學硏究所 2002 基礎科學硏究 Vol.15 No.2

본 연구에서는 갯녹음(백화)현상이 세균생태계와 어떤 관련이 있는지에 대한 기초자료 및 정보를 얻기 위하여 갯녹음 현상이 일어난 제주도 성산과 강정지역 연안의 해수에 존재하는 세균군을 16S rRNA 증폭기법을 이용하여 조사하였다. 강정지역에서는 Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacter, Sulfitobacter, Roseobacter 등 다양한 종류의 세균이 분포되어 있었으며, Alcanivorax가 20%의 빈도로 가장 많이 나타났다. 반면, 성산 지역에서는 Pseudomonas속 균주가 우점종으로서 존재하였으며, Pseudomonas tolaasii(혹은 Pseudomonas corrugata)와 유연관계가 가까운 세균은 44%, Pseudomonas mandeli와 가까운 세균이 24%, Verrucomicrobiales와 가까운 세균은 4%, 기타 동정되지 않은 세균은 세 group으로 구분되었으며, 각각 8%, 8%, 12%를 차지하여, 두 곳에 분포되어 있는 세균군상이 상당한 차이점이 있음을 확인하였다. 갯녹음 지역인 강정과 성산 해수의 8월(표품 채집시기)의 수온은 27℃-27.5℃, 염분의 농도는 30.24-30.60%, pH는 8.23-8.36, 용존산소량(DO)은 각각 7.20-7.28로, 두 지역에서 매우 비슷한 것으로 조사된 바 있는데, 이는 수온이나 염분의 농도, 또는 pH보다는 다른 원인에 의하여 두 갯녹음 지역에 분포하는 세균군의 차이를 가져왔음을 제시해 주었다. In this study, the bacterial communities distributed in sea water of the whitening areas of Gangjeong and Seongsan, Jeju-do have been analyzed using the PCR amplification of 16S rRNA to obtain fundamental data and information on relationship of the whitening phenomenon and microbial ecosystem. In Gangjeong, diverse bacteria such as Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacier, Sulfitobacter, and Roseobacier have been found, and Alcanivorax was the most abundant clone. The most abundant clone from Seongsan was Pseudoinonas, of which Pseudomonastolaasii and Pseudonionas mandeli were most abundantly occurred in the frequency of approx 44% and 24%, respectively. Approx 4% of the bacterial clones closest to firruconiicrobiales and other unidentified clones were also found in Seongsan, suggesting there is a great discrepancy between bacterial communities from the whitening areas of Seongsan and Gangjeong. The mean tem- perature, chlorine concentration, pH, and dissolved oxygen (DO) of the sea water of Gangjeong and Seongsan in August of 2001 (sampling period) was 27℃-27.5℃, 30.24-30.60%, pH 8.23-8.36,7.20-7.28 ㎎/ℓ, suggesting other environmental factors except for the factors mentioned above might result in difference of bacterial communities distributed in both areas.

敎科課程을 中心으로 한 韓日農學系(園藝·食品·獸醫)大學의 敎育現況 調査

李愚升,孫泰華,尹衡植,毛麒喆 경북대학교 1982 새마을 硏究論叢 Vol.2 No.-

The authors studied on the curicullum between Korea and Japan in the deaprtments of horiculture, food technology and veterinary. The results are as follows. 1. In the department of horticulture, professional education period was 3 years in Korea while 2 or 2.5 years in Japan. These results were compared among 3 colleges of Korea and Japan. Credits for graduation was 48-52% for propessional education with submajoring subjects and 45% when majoring subject in Korea, but 54-61% in Japan. Credits for required subjects were 17-29% among credits for graduation in Korea while 28-29% in Japan. 2. Cultural education period is almost 1 year in Korea, but different in Japan. Credits for graduation are as follows; Cultural education is 36-37%, majoring fields 15% and the others 12-13% in Korea while 43-46% for cultural education, 54-57% for majoring field in Japan. Differences were shown among 3 colleges dealt with the department of food technology by locational characteristics in Japan. 3. In the department of Veterinary, collage curicullums were not fixed for the students because of transitional period. Required subjects among 4 colleges were shown as the same tendency in Korea.