Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

사람 위선암에서 cathepsin L의 발현증가

홍원선,홍석일,박인철,손영숙,정훈용,양석균,김해련,민영일 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

cathepsin L은 lysosomal cysteine 단백분해효소로서 기저막(basement membrane)과 세포외기질(extracellular matrix)을 파괴하여 암세포의 침윤과 전이에 중요한 역할을 하는 물질로 알려져 있다. 이러한 cathepsin L에 대한 mRNA 발현도를 5개의 사람 위선암(gastric adenocarcinoma) 세포주와 5명의 위선암 환자에서 채취한 조직에서 방사능으로 표지된 cathepsin L특이 cDNA를 사용한 Northern blot법으로 측정하였다. 위암의 전이병소에서 수립한 세포주인 SNU-5, SNU-16, MKN-45와 Kato Ⅲ에서는 cathepsin L mRNA가 발현되었으나 원발병소에서 수립한 AGS 세포주에서는 mRNA의 발현이 관찰되지 않았다. 5명의 위암 환자에서는 원발병소, 전이가 확인된 임파절 및 암 근처 정상 위점막에서 각각 조직을 채취하여 cathepsin L mRNA의 발현을 측정하였다. 원발병소와 전이병소에서는 모두 cathepsin L mRNA가 발현되었으나 정상 위점막조직에서는 전예에서 mRNA 발현이 관찰되지 않았다. 한편 mRNA의 발현도는 1예에서는 전이병소가 원발병소에 비해 높았으나, 2예에서는 전이병소에서 발현도가 낮았으며, 나머지 2예에서는 원발병소와 전이병소 사이에 차이가 없어, 원발병소와 전이병소 사이에 mRNA의 발현도의 일관성 있는 경향은 관찰되지 않았다. 이상의 결과는 cathepsin L은 위암의 발생과 진행에 있어 암세포의 침윤과 전이를 촉진하는 것 이외에 또 다른 역할을 할 가능성을 시사하고 있다고 사료된다. Cathepsin L, a lysosomal cysteine protease, is known to play an important role in cancer invasion and metastasis by degrading the components of basement membrane and extracellular matrix. The mRNA expression of cathepsin L was determined by Northern blot analysis using a radiolabeled cDNA specific for cathepsin L in five human gastric adenocarcinoma cell lines and five surgical specimens of primary gastric adenocarcinomas, their metastatic lymph nodes and matched adjacent normal mucosae. The mRNA of cathepsin L was expressed in all of the four cell lines established from the metastatic sites, SNU-5, SNU-16, MKN-45 and Kato Ⅲ, while not detected in one cell line established from the primary site, AGS. The mRNA was expressed in all of the five primary and five metastatic cancer specimens tested, while it was not detected in all matched normal mucosae. The intensities of the mRNA expressions, however, did not show the consistent pattern between primary sites and metastatic lymph nodes. These results suggest that cathepsin L may have the other function in addition to facilitation of the invasion and metastasis during the development and progression of stomach cancer.

편측 폐환기에서 Prostaglandin F2α가 심혈관계에 미치는 영향

양홍석,조형상 중앙대학교 의과대학 의과학연구소 1990 中央醫大誌 Vol.15 No.1

The lung is usually considered to have one function, namely gas exchange. Another function of lung is pharmacokinetic function, which the cells and enzymes of pulmonary vascular bed act to change biological activities of a variety of substances transported to them through pulmonary circulation. The pulmonary vasoconstrictor response to hypoxia is a part of a self-regulatory mechanism by which capillary blood flow is automatically adjusted to alveolar ventilation. Some vasoactive substances mediate the hypoxic pulmonary vascular response, and prostaglandin F2a, which is used for uterine contraction during obstetric surgery, is considered to potentiate the hypoxic pulmonary vasoconstriction. The author investigated the cardiovascular effects of PF2α during one-long ventilation in Korean mongrel dogs. Dogs were anesthetized with pentobarbital, morphine and pancuronium and intubated with Rush endotracheal tube No. 6.5.. One-lung ventilation was induced by deep right endobronchial intubation, which was confirmed by chest movement and abscence of breathing sound in the left lung. Prostaglandin F2α in dose of 0.5, 1.0, 2.0 ㎍/kg/min was infused into peripheral vein. The results were as follows: 1. One-lung ventilation group was compared with control group: The heart rate was decreased and MPAP, PVR and SVR were increased, In blood gas, pH and PO2 were decreased but PvCO_2 and shunt were increased. 2 Prostaglandin F2α infusion groups were compared with one-lung ventilation group: The cardiovascular function and blood gas changes were not effected by prostaglandin F2α, but MPAP was increased with dose-dependent manner and PVR were increased in prostaglandin F2α in doses of 1.0, 2.0㎍/kg/min infusion group. Thus hypoxic pulmonary vasoconstriction was found to be enhanced b usual dose of prostaglandin F2α (0.5 ~ 2.0 ㎍/kg/min).

고빈도 환기

양홍석 중앙대부설한국의과학연구소 1985 한국의과학 Vol.17 No.3

안와내벽의 단독외향골절에서 골절정도와 안구함몰정도와의 관계

진홍률,신시옥,추무진,최영석,양상권,김종욱 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.5

쾌적한 캠퍼스 조성을 위한 기본계획 기초적 연구

梁富弘,朴碩鉉,崔生吉,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

In this study, the potential energy and limitation of the facility of Chungju national university are analyzed in the respect of human knowledge, nature, and visual environment. In addition, the effctiveness and problem of the existing master plan are evaluated through the investigation on the main hart and development procedure of the previous master plan of CJU. These results are compared and reflected to the plan of Vision 2003, e-STRR2010 and effective campus development plans of CJU are presented.

탄소성 변형을 받는 냉간 압연강의 X선 잔류응력 특성에 관한 연구

홍순혁,이동우,양광호,조석수,주원식 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.1

In textured material, diffraction angle 2θ usually shows a nonlinear relation against sin^2Ψ due to elastic anisotropy of crystals. SPHD and SPCD steel is cold-rolled carbon steel for automobile. The characteristics X-ray for stress measurement is Cr K_α and Mo K_α1characteristic X-ray. The sin^2Ψ-2θ diagram under elastic strain seems to have a linear behavior using regression line of data but has a nonlinear behavior in distribution of data by Cr K_αcharacteristic X-ray. As the plastic strain of specimen increases, the nonlinearity of 2θ with respect to sin^2Ψ increases remarkably. On the other hand, the diffraction angle 2 θ by Mo K_α1 characteristic X-ray shows a good linearity on sin^2Ψ-2θ diagram under plastic strain as well as elastic strain. Therefore, This paper presents the measurement of residual stress in cold-rolled carbon steel for automobile using penetration depth of Mo K_α1 charateristic X-ray and multiplicity factor of crystal diffraction plane.

술전 Aminotransferase의 측정과 분석평가 : SGOT and SGPT

양홍석,진홍용 중앙대부설한국의과학연구소 1986 한국의과학 Vol.18 No.1

영유아 섭식에 관한 영양교육이 어머니의 영양지식,태도 및 실천에 미치는 효과

안홍석,김미양 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

This study evaluated the effect of the nutrition education about infant feeding on the change of mother's knowledge, attitude and practice of weaning. The subjects consisted of treatment group (n=54) and control group (n=34). The treatment group participated in 6 times nutrition education and 5 times surveys. Nutrition education for weaning of the treatment group improved nutrition knowledge, attitude and practice in infant feeding. Before education, mean scores of control group and treatment group were 7.3 and 7.5 respectively but there was no significant difference. Both group got low score in a statement about weaning period, but they got good score in a statement about weaning method. After education, treatment group got better score(9.1) than control group(8.3) significantly (p.0.05). Treatment group was more desirable than control group specially in a use of Sun-sik, sugar, salt and weaning food mixed with formula after education (p<0.05). Practice of spoon feeding was more frequent in treatment group after education, however, control group used sugar and commercial weaning food more frequently than treatment group(p<0.05). Intake frequency of cow's milk and cookies of infants at age 9-12 month in control group was higher than treatment groups(p<0.01). Thus the nutrition education to mother seems to be effective in achieving desirable dietary behavior of infants.

혈액투석요법 환자의 우울, 불안 및 수면장애에 대한 연구

윤석준,양창국,한홍무 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 혈액투석요법을 받는 말기 신부전증 환자들은 불안이나 우울 등의 심인증상 외에도 여러 가지 수면장애를 호소할 수 있다. 말기 신부전증 환자에서 심인증상과 수면장애가 많을수록 삶의 질에 장애를 초래하며 혈액투석요법 도중 탈락이 높다. 저자는 본 연구에서 혈액투석요법 중인 환자의 우울, 불안 및 수면장애 등을 조사하여 향후 환자가료에 활용하고자 하였다. 방 법 : 부산시내 4곳의 혈액투석요법기관에서 혈액투석요법을 받는 말기 신부전증 환자 153명(남 87, 여 66)과 정신적, 신체적으로 건강한 동아대학교병원 행정직원 및 의과대학 학부모 113명(남 58, 여 55)을 대상으로 구조화된 면담과 자기평가척도를 실시하였다. 연구도구는 저자 등이 본 연구에 알맞게 개발한 인구통계학적 자료를 포함한 수면관련 설문지, 우울증 자가평가 척도인 Beck Depression Inventory(BDI), 불안 자가평가 척도인 State-Trait Anxiety Inventory(STAI)를 이용하였다. 결 과 : 환자군이 대조군에 비하여 신체증상 및 심인증상을 더 많이 호소하였고(p<0.05), 수면관련 불편을 더 많이 나타내었다(p<0.001). 환자군의 BDI 평균은 22.9로 대조군의 11.2보다 유의하게 높았다(p<0.001). BDI 점수를 기준으로 볼 때 남자환자 64명(74.7%), 여자환자 47명(71.2%)은 우울증군에 속하였다. STAI 평균 점수는 두 군간에 유의한 차이가 없었다. 환자군은 대조군에 비하여 입면잠복기와 수면 중 깨어 있는 시간이 더 길었고, 낮 동안의 졸리움 등 수면관련 불편을 더 많이 호소하고 있었다. 또한 환자군은 불면증(65.4%), 하지불편증후군(46.4%), 악몽(27.3%), 주기성사지운동장애(23.5%), 수면 식사장애(18.3%), 야경증(14.4%) 등을 유의하게 더 많이 호소하고 있었다(p<0.05). 결 론 : 혈액투석요법을 받고 있는 말기 신부전증 환자들은 대조군에 비해서 신체적, 심인적 증상을 유의하게 더 호소하고 불면증을 포함한 다양한 수면장애를 동반하고 있다. 또한 이들 사이에 높은 상호관계가 있는 바, 이는 혈액투석요법 증인 환자들에서 신체적 증상 및 심인적 증상뿐만 아니라 수면장애에 대한 치료적 접근이 필요함을 시사한다. Objectives : Psychological distresses and complaints in sleep, in addition to physical distresses such as pruritus and bone pain, are common in patients with hemodialysis. The purposes of this study were to investigate 1) the severity of physical distresses, 2) the severity of depression and anxiety, 3) the sleep disturbances, and 4) the correlation of the above variables in patients with hemodialysis. Methods : The patients with hemodialysis(male 87, female 66) and controls(male 58, female 55) completed a self-administered questionnaire package, which included Beck Depression Inventory, Spielberger's State-Trait Anxiety Inventory, and questions characterizing the reported sleep problems and quantitating the severity of the self-perceived physical and psychological conditions with linear visual analogue scales. Results : The results indicated that patients with hemodialysis complained of more physical distresses, more depressed mood, and more sleep disturbances suggesting insomnia, restless legs syndrome, periodic limb movement disorder, nightmare and obstructive sleep apnea syndrome, etc. There were significant positive correlations of physical distresses, depressed mood and sleep disturbances. However, there were no significant differences in state and trait anxiety between both groups. Conclusions : The authors suggest that the quality of life in patients with hemodialysis