Identifying risk factors for a-based diagnosed post-lymphadenectomy lower-extremity lymphedema in patients with gynecologic cancers

( Mi Seon Kim ),( Byung Su Kwon ),( Jin Young Choi ),( Myong Cheol Lim ),( Dong Hoon Suh ),( Ki Dong Kim ),( Jae Hong No ),( Yang Beam Kim ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

미강추출물을 이용한 곤충병원성 곰팡이 Beauveria bassiana의 최적 배양조건 및 효소활성

김창수(Chang-Su Kim),이중복(Jung-Bok Lee),김범수(Beam-Soo Kim),이민혜(Min-Hye Lee),강경묵(Kyeong-Muk Kang),주우홍(Woo-Hong Joo),김진원(Jin-Won Kim),임대준(Dae-Joon Im),권기석(Gi-Seok Kwon) 한국생명과학회 2013 생명과학회지 Vol.23 No.8

본 연구에서는 온실가루이 방제를 위하여 감염된 이병체로부터 균주 분리 및 농촌진흥청 및 경상북도농업기술원에서 균주를 분양 받아 5 종의 균주를 확보하여, 최종적으로 활성이 가장 높은 분리균주 M130을 선발하였다. Beauveria bassiana M130을 이용하여 균주가 갖는 효소학적 활성을 분석하였다. Chitinase와 protease의 활성을 조사한 결과, 타 균주에 비하여 높은 효소학적 활성을 가지는 조건을 얻었으며, 선발된 균주의 포자생산의 최적화를 위한 방안으로 PDB (potato dextrose broth)배지와 미강추출물을 이용한 ERBM (extracted rice bran medium)을 비교하여, 더 높은 생육도와 포자 생성량을 보인 ERBM을 본 균주를 배양하는 기본배지로 선발하였다. ERBM을 이용한 액체배양시의 최적조건으로, 기본배지인 ERBM에 0.5% (NH₄)₂SO₄를 첨가하여 pH 5, 28℃에서 배양최적화를 하였다. 향후 곤충병원성곰팡이 Beauveria bassiana M130의 최적화 조건에 따라 배양을 한다면 포자의 생산량 및 방제효과가 높아질 것으로 사료된다. The greenhouse whitefly, Bemisia tabaci, is considered one of the most destructive pests of crops. In this study, we aimed to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana M130 using rice bran. The optimal initial pH for the spore production of B. bassiana using extracted rice bran medium was 5.2 and 28℃. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 0.5% (NH₄)₂SO₄, with extracted rice bran 8:1. Using this medium, a production level of 2.15×10<SUP>9</SUP> spores per ml was obtained after six days from culture inoculation at 28℃ in a rotary shaking incubator at 130 rpm. In addition, the specific activities of extracellular enzymes of chitinase and protease were 4,296μmol and 375 μmol, respectively. These results suggest that Beauveria bassiana M130 could be a biocontroller for the greenhouse whitefly.

Priming Effect of Rice Seeds on Seedling Establishment under Adverse Soil Conditions

Lee, Suk-Soon,Kim, Jae-Hyeun,Hong, Seung-Beam,Yun, Sang-Hee,Park, Eui-Ho The Korean Society of Crop Science 1998 Korean journal of crop science Vol.43 No.3

An experiment was carried out to find out the priming effects of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) on. the seedling establishment and early emergence under excess soil moisture conditions. Seeds were primed by soaking in -0.6 MPa polyethylene glycol (PEG) solution at $25^{\circ}C$ for 4 days. The primed seeds were sown in soils with various soil moistures (60, 80, 100, 120, and 140% field capacity) at 17 and $25^{\circ}C$, respectively. Germination and emergence rates, plumule height, and radicle length of primed seeds were higher than those of untreated seeds at any soil moisture and temperature examined. The time from planting to 50% germination ($T_{50}$) of primed seeds was less than that of untreated seeds by 0.9~3.7 days. Germination rate, emergence rate, plumule height, and radicle length were highest at the soil moisture of 80% field capacity among the soil moistures. Priming effects of rice seeds on germination and emergence rates were more prominent under the unfavorable soil moistures (60, 100, 120, and 140% field capacity) than those under the optimum soil moisture condition (80% field capacity). However, priming effects on seedling growth were greater at near optimum soil moisture compared with too lower or higher soil moistures. Therefore, these findings suggest that priming of rice seeds may be a useful way for better seedling establishment under the adverse soil conditions.

CMOS 집적회로 테스팅을 위한 내장형 전류 감지 회로 설계

김태상,홍승호,곽철호,김정범,Kim, Tae-Sang,Hong, Seung-Ho,Kwak, Chul-Ho,Kim, Jeong-Beam 한국전기전자학회 2005 전기전자학회논문지 Vol.9 No.1

This paper presents a built-in current sensor(BICS) that detects defects in CMOS integrated circuits using the current testing technique. This circuit employs a cross-coupled connected PMOS transistors, it is used as a current comparator. The proposed circuit has a negligible impact on the performance of the circuit under test (CUT) and high speed detection time. In addition, in the operation of the normal mode, the BlCS does not have dissipation of extra power, and it can be applied to the deep submicron process. The validity and effectiveness are verified through the HSPICE simulation on circuits with defects. The area overhead of a BlCS versus the entire chip is about 9.2%. The chip was fabricated with Hynix $0.35{\mu}m$ 2-poly 4-metal N-well CMOS standard technology. 본 논문에서는 전류 테스팅을 이용하여 CMOS 집적회로에 존재하는 결함을 검출하는 내장형 전류 감지회로를 설계하였다. 이 회로는 일반적인 CMOS 공정으로 구현하였으며 결함전류와 기준전류를 전압으로 변환시켜 시험대상 회로의 결함을 고속으로 검출하며, 미세공정에도 적용가능한 회로이다 제안한 전류 감지회로는 전류원 내장으로 인한 추가적인 전력소모를 문제를 해결하였다. 제안한 회로의 정당성 및 효율성은 HSPICE를 이용한 시뮬레이션으로 그 타당성을 입증하였다. 제안한 전류 감지회로가 칩의 전체 면적에서 차지하는 면적소모는 시험대상회로에서 약 9.2%로, 내장형 전류 감지회로에 의한 면적소모는 무시할 만 하다. 제안한 회로는 Hynix O.35um 2-poly 4-metal N-Well 표준 CMOS 공정으로 제작하였다.

S-100 기반의 빅 데이터 연계 시스템 모델링 연구

김윤수(Youn-soo Kim),박순호(Shun-ho Park),홍순근(Soon-geun Hong),이형범(Hyeong-beam Lee) 한국통신학회 2017 韓國通信學會論文誌 Vol.42 No.11

오스템퍼링 처리한 회주철의 마멸특성에 미치는 잔류 오스테나이트 조직의 영향에 관한 연구

신호철,이종훈,김홍범,김창규,최창옥 ( Ho Chul Shin,Jong Hoon Lee,Hong Beam Kim,Chang Gyu Kim,Chang Ock Choi ) 한국주조공학회 1994 한국주조공학회지 Vol.14 No.6

N/A Grey iron containing a small amount of Cu and Mo to improve the effect of heat treatment and microstructures were poured in to the mold and them austenized at 900℃. After austenitizing the specimens of castings were austempered 210℃, 250℃, 300℃ and 350℃. The effects of matrix structures, mechanical properties and wear characteristics were investigated by austempering temperatures. Tensile strength and hardness of austempered grey iron are increased and the amount of retained austenite is decreased as austempering temperature is lower even though the amount of retained autenite in it only 4%. The amount of rolling wear loss are increased as rolling revolution is increased and wear loss of austempered grey iron under dry rolling condition is characterized by three models; initial wear, stationary wear and abnormal wear. It has been found that the amount of wear loss was increased with increasing maxium compressive stress and rolling revolution.

Bacillus sp. CS-52를 이용한 고추 탄저병 (Colletotrichum gloeosporioides) 방제 특성

권정자,이중복,김범수,이은호,강경묵,심장섭,주우홍,전춘표,권기석,Kwon, Joung-Ja,Lee, Jung-Bok,Kim, Beam-Soo,Lee, Eun-Ho,Kang, Kyeong-Muk,Shim, Jang-Sub,Joo, Woo-Hong,Jeon, Chun-Pyo,Kwon, Gi-Seok 한국미생물학회 2014 미생물학회지 Vol.50 No.3

본 연구에서는 고추재배 지역의 오염지 토양으로부터 탄저병원균인 Colletotrichum gloeosporioides에 대하여 항진균 활성이 우수한 균주를 분리하였다. 분리 균주의 생화학적 특성을 조사한 결과 내생포자를 형성하는 Gram-positive이며, 세포크기는 $2.5{\sim}3.0{\times}1.5{\mu}m$으로 짧은 간균으로 siderophore, cellulase와 IAA (Indole-3-acetic acid)을 생산하였다. 16S rRNA 유전자염기서열 분석 결과 Bacillus sp.와 99%의 상동성을 나타내어 Bacillus sp. CS-52로 명명하였다. Bacillus sp. CS-52의 항진균 활성 물질을 생산하기 위한 배양 최적 조건은 0.5% glucose, 0.7% $K_2HPO_4$, 0.2% $K_2HPO_4$, 0.3% $NH_4NO_3$, 0.01% $MnSO_4{\cdot}7H_2O$, 0.15% yeast extract, pH 7과 $30^{\circ}C$로 조사되었으며, 최적화된 배양조건에서 36시간에 최대 성장을 보이며, 고추 탄저병원균에 대하여 60시간 배양조건에서 가장 높은 13.3 mm의 항진균 활성을 보였다. 포자발아억제력은 48시간에 가장 높은 억제력이 보였고, siderophore는 최종배양시간 72시간까지 생성됨을 확인하였다. 식물생장조절물질 IAA와 활성효소인 cellulase의 경우 배양시간 24시간에 최대 생성됨을 확인하였으며, C. gloeosporioides에 대한 실내에서의 항진균활성 검증결과 화학농약 보다 더 높은 70%의 방제가를 나타내었다. 향후 Bacillus sp. CS-52 균주와 배양액을 이용하여 생물학적 친환경방제제로의 제품화가 가능할 것으로 사료된다. This study was carried out in order to develop a biological control of anthracnose of red pepper caused by fungal pathogens. In particular, this study focuses on the Colletotrichum species, which includes important fungal pathogens causing a great deal of damage to red pepper. Antagonistic bacteria were isolated from the soil of pepper fields, which were then tested for biocontrol activity against the Colletotrichum gloeosporioides anthracnose pathogen of pepper. Based on the 16S rRNA sequence analysis, the isolated bacterial strain CS-52 was identical to Bacillus sp. The culture broth of Bacillus sp. CS-52 had antifungal activity toward the hyphae and spores of C. gloeosporioides. Moreover, the substances with antifungal activity were optimized when Bacillus sp. CS-52 was grown aerobically in a medium composed of 0.5% glucose, 0.7% $K_2HPO_4$, 0.2% $KH_2PO_4$, 0.3% $NH_4NO_3$, 0.01% $MnSO_4{\cdot}7H_2O$, and 0.15% yeast extract at $30^{\circ}C$. The inhibition of spore formation resulting from cellulase, siderophores, and indole-3-acetic acid (IAA), were produced at 24 h, 48 h, and 72 h, respectively. Bacillus sp. CS-52 also exhibited its potent fungicidal activity against anthracnose in an in vivo test, at a level of 70% when compared to chemical fungicides. These results identified substances with antifungal activity produced by Bacillus sp. CS-52 for the biological control of major plant pathogens in red pepper. Further studies will investigate the synergistic effect promoting better growth and antifungal activity by the formulation of substances with antifungal activity.