폐경기 여성에서 호르몬 대치 요법시 병행 투여된 프로제스토젠 투여 방법에 따른 골대사의 생화학적 지표에 미치는 영향

이권해,강선영,임경빈,이해혁,구도형,이석민 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Purpose : To evaluate the impact of added progestogen on biochemical markers of the bone turnover undergoing hormone replacement therapy in the postmenopausal women. Materials and Methods : There are 71 postmentopausal women who are divided into three groups (Group I, Group II, Group III). Group I(continuous combined)is given conjugated equine estrogen (CEE, 0.625mg, day 1-30) plus medroxyprogesterone acetate (MPA, 2.5mg, day 1-30), Group II(unopposed estrogen) is given conjugated equine estrogen (CEE, 0.625mg, day 1-30) plus medroxyprogesterone acetate (MPA, 5mg, day 1-12). And there were measured serum osteocalcin, serum total alkaline phosphatase and urinary deoxypyridinoline excretion before starting hormone replacement therapy and 12 months later. Results : The serum osteocalcin, serum total alkaline phosphatase and urinary deoxypyridinoline excretion significantly decreased after hormone replacement therapy (Group, I, II, III). And there was no significance in decreases in biochemical markers of the bone turnover among three groups. Conclusions : It was concluded that the impact of added progestogen on the biochemical markers of the bone turnover undergoing hormone replaced therapy in postmenopausal women was not significant than nothing added group. Thus we concluded that progestogen was not impact on decreasing on the biochemical markers of the bone turnover in postmenopausal women undergoing hormone replacement therapy.

사람 Ferritin 유전자를 이용한 담배의 형질전환

이주안,김형석,임채완,김해영,박영두 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-

사람 H-chain ferritin 유전자를 Agrobcterium-mediated transformation 방법으로 담배(Nicotiana tabaccum L)에 전이시켰다. 이를 위하여 사람 H-chain ferritin 유전자를 C_(S)VMV promotor와 NOS terminator를 운반하는 plant binary vector, pILTAB 357에 도입하였다. pILTAB 357를 포함하는 Agrobcterium tumefaciens LBA4404로 담배 잎 절편체를 감염시킨 후 형질전환 신초는 MS배지에 1 5 ㎎/L BA, 50 ㎎/L kanamycin 그리고 200 ㎎/L cefotaxim이 첨가된 1차 선발배지에서 유도하였다. 유도된 신초는 MS배지에 100 ㎎/L kanamycin과 200 ㎎/L cefotaxim이 포함된 2차 선발배지에서 뿌리를 유기하였다. 뿌리가 형성된 재분화 개체는 온실에서 재배하였으며 자가수분하여 종자를 수확하였다. 사람 H-chain ferritin유전자의 담배 genome내로의 전이여부를 H-chain ferritin 유전자내의 primer를 이용하여 PCR 방법으로 확인한 바 형질전환 개체에서 예상했던 560bp 단편을 볼 수 있었으나 형질전환을 시키지 않은 식물체에서는 PCR 산물을 확인할 수 없었다. Kanamycin 저항성 분석은 전이유전자의 후대로의 안정적인 유전을 보여 주었으며 저항성 종자들은 homozygous개체를 확보하기 위해 온실에서 재배 중에 있다. A human H-chain ferritin gene was introduced into tobacco (Nicotiana tabaccum L.) plants by Agrobcterium-mediated transformation. To introduce this gene, human ferritin gene was cloned into plant binary vector, pILTAB 357, horboring CsVMV promotor and nopalin synthase terminator (3' NOS). After infection with Agrobacterium tumefaciens LBA4404 containing pILTAB 357, transformed shoots were induced from first selection media (MS media + 1.5 ㎎/L BA + 50 ㎎/L kanamycin + 200 ㎎/L^(1) cefotaxim). Induced shoots were rooted in second selection media (MS media + 100 ㎎/L kanamycin + 200 ㎎/L cefotaxim). Rooted shoots were cultivated, selfed, and seeds were harvested in the green house. To confirm the transfer of the human H-chain ferritin gene in the genome of tobacco plants, PCR was conducted using specific primers of the H-chain femritin gene. A PCR fragment of 560 bp was obtained from each transformed plants, which is the predicted size of the H-cham ferritin gene but not from nontransformed plants. Kanamycin resistance assay showed that transgenes were stably inherited to next generation in all lines. These resistance seeds from each line were cultivated in the green house to obtain homozygous lines for further study.

선천성 판상 피부 골종 1예

이미애,김진형,황규광,박혜진,조소영 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.7

Osteoma cutis is a rare, benign, frequently congenital tumor of the skin composed of bone tissue, showing neither invasive nor metastasizing tendency. The term osteoma cutis is applied to cases of primary cutaneous ossification in which there is no evidence of Albrights hereditary osteodystrophy. Congenital platelike osteoma cutis is a clinically different variant of primary cutaneous osteoma. We report the case of a 10-month-old male infant who presented with two walnut-sized firm, poorly demarcated plaques containing pinhead-sized erythematous papules on the abdomen and the right leg and multiple variably sized erythematous subcutaneous nodules on the left leg sinee birth. The initial lesions did not enlarge nor did new lesions develop. A biopsy specimen of the abdominal lesion showed bony plate and cartilaginous structures in the subcutaneous fat tissue. There was no history of any skin disease prior to the development of osteoma.

AGV와 作業物에 대한 日程計劃

李永海,吳瀅述 漢陽大學校 産業經營硏究所 1989 産業經營硏究 Vol.1 No.-

Which machine processes the first operation of some jobs will affect the subsequent job scheduling under automated manufacturing system environment with alternative routings allowed. This study presents the heuristic algorithm which can decide the routings in consideration of alternative machines in the case of two alternative machines.

산업보건관리자를 위한 소음성 난청 예방지침서의 개발

이원철,유경혜,장성실,이세훈,구정완,김현욱,노영만,이경재,김형아 가톨릭 대학 산업의학 쎈타 산업의학연구소 1996 韓國의 産業醫學 Vol.35 No.1

Occupational hearing loss became the leading occupational disease by constioution 56% of all work-related diseases in Korea. However, the prevalance rates showed wide variations among the specific medical examination providers and were very low(0.8-1.4%) compared with those 8.4% in Japan. The sources of these variations was regarded in the preview study as 1) Employee audometric estings were primarily conducted by nurses and nurse-aids who had no formal training for audiometry; 2) althought audiometric testing equipment used by the spedific medical examination providers, it should be periodically calibrated, a significant number of them were not properly calibrated : 3) Initial audiometric testings were conducted at any available spaces in workplace or at the testing room without considering bachground noise level. One of solutions that can be done for sloving surrent noise related problems is to provide a noise related manual for the occupational health managers including doctors, nurses and hyginests. This study was done to provide such a manual. The main core of the manual was hearing conservation program. And the contents of the manual was the same as the main frame of hearing conservation program such as noise survey, provided of the diagram showing the core contents of each factor of the hearing conservation program. Hopefully, this manual will be the references of the standardized performances of the specific medical examination providers.

미소압입시험법과 SEM/EPMA를 이용한 치과용 금속-세라믹 시스템의 계면특성 연구

이해형,송윤희,이수종,Yokoyama, Gen,Akimoto, Naotake,Hirano, Susumu 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.2

The purposes of this study were to investigate the interface area of dental metal-ceramic systems by using nanoindentation technique and SEM/EPMA. Gold (PFG) and Ni-Cr-Be alloy (PFM) coupons (10×10×1 ㎜) were cast and veneered with Ceramco Ⅱ opaque and body porcelain. Specimens tired once (one-tiring) and nine times (nine-firing) of body porcelain were prepared and compared in this study. The metal-ceramic interfaces embedded in epoxy resin were exposed and received 0.25-㎛ finish. Nanoindentation test (ENT-1100a, Elionix, Japan) was conducted at the interlaces with a load of 100 ㎎f (0.98 mN). Sixty indentations were made at two specimens of each metal-ceramic group. Nanoindentation marks were confirmed to allot their positions under a reflected microscope. Mean indentation hardness and elastic modulus values were determined at the area of metal area >5㎛ (M) and <5㎛ (MI) from interface, and porcelain area (P). Indentation hardness value was also compared with Knoop hardness values. Distribution of trace elements in alloys was examined at the metal-ceramic interfaces through X-ray mapping obtained from SEM/EPMA. From this study, following results were discussed. Mean indentation hardness values of gold alloy and porcelain were agreed with their Knoop hardness data, except for the base metal. There were no abrupt changes of mechanical property at the interlace areas of PFG and PFM specimens, although transition area of mechanical properties was not observed clearly at the interlace However, elastic modulus values obtained by nanoindentation were higher than their respect literature values. These results were probably caused by residual stress arisen at the metal-ceramic area, even though further study should be considered. High resolution SEM/EPMA study successively revealed fine mapping of trace elements at the metal-ceramic interfaces. A high concentration of Indium was observed at the gold-ceramic interface. Beryllium was not concentrated at the interface of PFM, although they appeared in NiBe phase. Generally, mechanical properties and distribution of trace elements of metal-ceramic interface were not significantly changed with increased firing times. Nanoindentation technique was useful to analyze interface properties of metal-ceramic systems.

생약재 첨가 사료를 투여한 넙치(Paralichthys olivaceus)의 비특이적 면역반응, 혈액성분 및 항병력 효과

정승희,이주석,한형균,전창영,이해영 한국어병학회 2002 한국어병학회지 Vol.15 No.1

본 연구는 식물성 생약재의 열수추출이 농도별로 첨가된 사료를 넙치(Paralichthys olivaceus)에 경구 투여한 다음 비특이적 면역반응 및 질병 저항성의 향상여부를 알아보고자 하였다. 실험에는 약쑥과 삼지구엽초를 7:3(w/w)의 비율로 열추출한 후, 0%, 0.1%, 0.5% 및 1%의 농도로 첨가하여 만든 2종류 혼합생약재(2-혼합구)와 약쑥, 삼지구엽초, 연교, 구절초, 박하, 지유, 삼백초, 상백피, 팔각의 9종류 생약재가 동일한 농도로 열수추출한 후 제작한 9종류 혼합생약재(9-혼합구)를 사용하였다. 이들 농도별 첨가사료를 넙치(평균 10.3±2.5g)에 12주 동안 경구 투여하면서 혈청내 라이소자임 활성, 혈청의 Escherichia coli에 ??살균능력 및 혈액학적 변화를 조사하였다. 그리고 최종 투여 12주 후에 넙치 에드와드병의 원인균 Edwardsiella tarda의 생균 공격실험을 실시하여 상대생존율(RPS)로써 저항성 향상효과를 살펴보았다. 2-혼합구 0.1%는 비특이적 면역활성을 크게 증가시켰으며, 상대생존율이 67%로 높게 나타나 가장 효과적인 질병 저항성 향상효과를 보였다. 따라서 2-혼합구 0.1%는 넙치 체내 비특이적인 면역기구를 자극함으로써 어체의 자연저향성을 증강시켜 세균성 질병의 감염에 대한 방어력을 상당히 향상시킬 수 있을 것으로 사료된다. 9-혼합구 0.1% 및 0.5%는 비록 비특이적 면역활성은 증가시키지 못하였으나, 상대생존율은 각각 53%를 나타내어 질병 저항성을 일부 향상시키는 효과가 있었다. Effects of medicinal herb extract on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Wormwood, Artemisia asiatica NAKAI and barrenwort, Epimedium koreanum NAKAI were mixed at a ratio of 7 : 3 (w/w) for 2-herbs extract and wormwood, barrenwort, Korean forsythia, Forsythia koreana NAKAI, chrysanthemum, Chrysanthemum zawadskii var. latilobum KITAMURA, peppermint, Mentha arvensis L. var, piperascens MALINV., great burnet, Snaguisorba afficinalis L., Lizard tail. Saururus chinensis BAILL., mulberry, Morus alba L., and star anise, Illicium varum HOOK, f, at the same weight for 9-herbs extract. Two-herbs of 9-herbs extract were prepared by heating after adding 10㎖ of distilled water per g of the herb mixtures. Fish (10.3±2.5g) were fed the experimental diets supplemented with the 2-herbs or 9-herbs extract at the different concentrations of 0%, 0.1%, 0.5% and 1% per kg diet for 12 weeks. Lysozyme and bactericidal activities of serum, and hematological characteristics were examined during experimental period. After feeding test period, all experimental groups were challenged with E. tarda. Lysozyme activity from the fish fed the diet supplemented with 0.1% or 0.5% of 2-herbs extract was significantly higher than the control. But there was no difference both in bactericidal activity and hematology among each group. Sixty seven % of relative percent survival values (RPS) in the group fed the diet supplemented with 0.1% of 2-herbs was higher than the other group and the control. These results suggest that supplenmentation of 0.1% of 2-herbs extract to a commercial diet may enhance disease resistance in olive flounder. Although both 0.1% and 0.5% 9-herbs extract did not improve non-specific immune reponses, they could enhance disease resistance of 53% RPS, respectively.

광도 변화가 광중합형 컴포머와 컴포짓트 레진의 중합도 및 불소 유출에 주는 영향

최재윤,이해형,임범순,이용근,신현철 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.4

The purpose of this study was to investigate the fluoride releasing behavior of one self-curing glass-ionomer (Fuji II), one light-curing glass-ionomer (Fuji II LC), three compomers (PrismaFlow, UniFil F, and Dyract AP) and SIX composite resins (Flowline, Fissurit FX, Flow-It, Wave, Heliomolar Flow, and Glacier) as a function of light intensity. Disk type specimens were stored in deionized water at 37℃ for 1, 2, 4, 8 hrs and 1, 2, 7, 14, 21, 28 days. The deionized water was changed after measurements. The amount of fluoride ion, which was released from specimens, was measured by pH/ISE Meter (Istek Model 730P, Korea) with fluoride electrode. The results were statistically compared by Tukey multiple comparisons test (p=0.05). There were significant differences in degree of polymerization between specimens with 1 hr after irradiation and those with 24 hrs after irradiation (p<0.05). The cumulative amount of fluoride released was decreased with increasing light intensity (or degree of polymerization). The fluoride-releasing patterns of specimens were significantly different (p<0.05).

광조사 시간에 따른 복합레진과 Ormocer의 중합수축에 관한 연구

정연정,민경산,이해형,조경모,조용범 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.4

Ormocer has organic-inorganic compound polymers. One of advantages of ormocer is reduced polymerization shrinkage. The purpose of this study was to compare the amount of contraction shrinkage of composite resins and ormocers. Additionally, the time of each material when there is no further change of contraction shrinkage was analysed. Four brands of composite resins (P-60, Surefil, Z-250 and Denfil) and two brands of ormocers (Definite and Admira) were used. 20 seconds, 40 seconds and 60 seconds of curing times were given. Contraction shrinkage of them were measured using a linometer for 80 seconds. The effect of material and curing time to contraction shrinkage at the time of 80 seconds was analysed by two-way ANOVA. The effect of time to contraction shrinkage was analysed by one-way ANOVA, and the time when there was no further change of the contraction shrinkage was analysed. The results are as follows : 1. P-60, Dfinite, Z-250 and Denfil had no further change of contraction shrinkage from the time of 20 seconds, and Surefil and Admira had no further change of contraction shrinkage from the time of 10 seconds. 2. Statistical analysis revealed volumetric shrinkage varied among material (p<0.05). No significant difference of contraction shrinkage among different curing times was found, and there was no effect of interaction between materials and curing times to contraction shrinkage. 3. Dfinite and Admira showed the statistically same contraction shrinkage with those of Z-250 and P-60, which is higher than that of Surefil and lower than that of Denfil (p<0.05).

측정방법에 따른 치과용 수경성 시멘트의 경화시간

박정우,기형춘,변상기,이해형 大韓齒科器材學會 2001 대한치과재료학회지 Vol.28 No.4

Information on setting behavior of luting cements is essential to accomplish successful cementation of dental restoration. The purpose of this study is to investigate the influence of test variables on the setting time of dental water-based luting cements. Six kinds of luting cements were investigated: two zinc phosphate, two zinc polycarboxylate and two glass ionomer cements. Cements were mixed with the standard consistency for luting according to their respective manufacturer's recommendation. Setting time of cements was measured with two test apparatus (penetrating indentor and oscillating rheometer) under one of following environmental conditions (temperature/humidity); 37℃/90%, 23℃/50% and 37℃/20%. The Setting time was recorded as the time elapsed from start of mixing to the time indicated at the ISO specification (9917:1991) or when the oscillation is reduced to 95% of the original width. Setting time of luting cements was decreased with increase in temperature and decrease in humidity, and it was significantly influenced by environmental temperature and humidity. Some cements tested showed significant differences in setting time measured between indentor and rheometer under laboratory environment. Percent water loss of cements was different under various environmental conditions. This was largely arisen at the early stage of setting. Zinc Phosphate was hygroscopic under high humidity. these results indicate that working and setting time should be separately evaluated in office and oral environments, respectively. Oscillating rheometer was useful to investigate setting behavior of dental water-based luting cement. However, control of test environment and sensitivity should be considered in the rheometry.