Rotavirus 전기영동형별을 위한 RNA 추출과정 및 조건의 비교연구

조은경,김은순,양재명,김경희,조양자 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

Rotaviruses (Rv) are the major cause of severe dehydrating dearrhea among young children in Korea, where they are frequently responsible for 68% of the cases requiring hospitalization. Laboratory diagnosis of rotaviral infection in Korea depends mainly on enzyme-linked immunosorbent assay (ELISA) that employs expensive commercial diagnostic kits purchased from abroad and that shous the presence or absence of group A Rv only. PACE have however provided epidemiologic evidence to demonstrate the sxistence of different strains (both group A and atypical Rv) during diarrgeal outbreak, the appearance of new strains and desappearance of old ones from a community, shifts in the prenalence of strains, and the persistance of a single strain in newborns. For such reasons, we have felt that it is essential to raise the sensitivity and shorten the processing time for PAGE. We therfore have devised a rapid method for Rv RNA extration by directly extracting the Rv RNA from the specimens (that is, skipping freon extraction and running as ultracectrifuge) and using an urea-containing dissociation mixture to dissolve dried RNA for PAGE. We have also shorten the PAGE diagnostic results by sequential staining of the same gel with ethidium bromide and silver stain. With the use of the rapid method, it was feasible to increase the efficacy of the PAGE with reduced cost and working time. Phenol extration of stools, however, was essential in maintaining the sensitivity of the method.

모유수유 실태 및 모유수유에 영향을 미치는 요인에 대한 조사

양은석,문경래,박종 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Human milk is recognized as the optimal diet for newborn and infants. The ?? of breast-feeding has increased in many countries, but in Korea is still very low. We ??veyed the present status of infant feeding methods and the reason for the failure of breast-feeding. The survey was performed by a special questionarie on 1345 cases of mothers who visited both the well baby clinic of Chosun University Hospital and local clinics in Kwangju from May to June. 1993. The results obtained are as follows: 1) The rates of breast-feeding, mixed-feeding, formula-feeding were 27.4%, 21.6%, 51.0% respectively. 2) The rate of breast-feeding was higher in babies of mothers of older age. lower eduacation level, and normal vaginal delivery. 3) Mothers recognized the significance of breast-feeding(95%) , but the rate of breast-feeding was very low. 4) Knowledge about breast-feeding was mainly from books(29.7%), parents(27%), TV and radio(20.3%), and only(9.7%) marginally from medical doctor. 5) The cause of failure of breast-feeding was the mother's concern of insufficient breast milk(48.6%), breast engorgement and pain of the nipples(16.3%), diarrhea(10.3%). We strongly recommend that pediatricans take a more forceful role in increasing the rate of breast-feeding. Since. as our study showed, the obsticales to breast-feeding are based in misinformation, the pediatrician through education and reinforcement is in a strong position to bring about change.

영아 천식의 임상적 고찰 : Asthma prospective score에 의한 Based on Asthma Prospective Score

양은석,노영일,문경래,박영봉,박상기,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

A Clinical study was made on 40 patients for infantile asthma less than 2 years old who had been admitted to the Department of Pediatrics of Chosun University Hospital from January 1997 to December 1998. We evaluated family history of allergic diseases, clinical features, serum IgE level, eosinophilia, chest radiologic findings and response to bronchodilator. The results obtained were as follows ; 1) The male to female ratio of patients studied was 4 : 1. 2) Eighteen of the 40 patients (45.0%) had a family history of allergic diseases, of which 10 patients had maternal allergic diseases. 3) Atopic dermatitis was the most common disease (37.5%) among the associated allergic diseases. 4) Thirty two of the 40 patients (80.0%) showed high serum total 19E levels. 5) Only 5 of the 20 patients who were examined by MAST showed positive reaction. 6) The frequency of chest radiologic findings observed in these patients was as follows ; hyperinflation (52.5%), increased bronchovascular marking(30.0%), no pathologic findings(25.0%), and pneumonic infiltration(25.0%). 7) In response to bronchodilator 28 cases(70.0%) were effective. 8) When we divided the 40 patients into two groups by the diagnostic criteria of Asthma prospective score (AP score), 30 patients(75.0%) were included in a compatible group. On the basis of AP score, family history of major or minor allergic dieases and the responsiveness to bronchodilator were important factors for the diagnosis of asthma.

물가자미 液化蛋白質 濃縮物의 製造 및 品質安定性

梁升澤,朴殷京 경성대학교 1987 부산수산대학 논문집 Vol.8 No.3

This study was designed to prepare liquefied roundnose flounder protein concentrate with a long self life. The sample was divided into 2 groups of whole fish and fillet. The whole roundnose flounder was chopped and mixed with same amount of water and then hydrolyzed by bromelain, papain and complex enzyme. The optimum conditions for hydrolysis of whole fish were 50℃, 4 hours by bromelain (0.5%) digestion, 55℃, 5 hours by papain (0.3%) digestion, and 55℃, 4 hours by complex enzyme (3.0%) digestion, respectively. The liquefied whole roundnose flounder protein by complex enzyme could be decolorized by the treatment with 15% activated charcoal at room temperature for 30 minutes. The decolorized hydrolysates from whole fish and fillet were concentrated to brix 25% and then dried with spray dryer in conditions of inlet temperature 185℃, outlet temperature 85℃. The products were packed in cans and checked the keeping quality such as solubility in water, soluble nitrogen, relative viscosity, emulsifying stability, color, and sensory evaluation during storage for 3 months at 5℃, 20℃, and 35℃. Both of the products had an excellent functional properties. Good quality of products was maintained until 3 months storage at 5℃ and 20℃ in both samples, but it had been lost after 15 days and 1 month at 35℃ in liquefied whole fish and fillet protein concentrates, respectively. The keeping quality of products from fillet were better than those of products from whole fish.

1950년대 서울番地案內圖와 서울商界略圖에 대한 지도학 및 역사지리학적 고찰

양보경,장은미 서울시립대학교 부설 서울학연구소 2005 서울학연구 Vol.- No.24

This paper aims to analyze Seoul Address Map published in 1956 and Seoul Commercial Map published in 1955 after Korean War. Two maps are converted into a raster format and partly vectorized to explore the contents of maps in cartographic aspects and advertisement items depicted with the map in historical-geographical aspect. The Seoul Address Map map is being housed at the map library of University of Kansas, while 1955 map at the map library at Yale University. Published by a private company (Daehan Annae Sa), it is different from the previous maps published (luring the period of Japanese conquest. The Seoul Address Map inclined about 45.58˚(stdev= 0.997) for Jongro street and 44.5479 (stdev= 3.031) for Euljiro from the horizontal line while the Seoul Commercial Map has the same inclination with modern digital maps and address Map published in 2001 with north side up. The Seoul Address Map does show less pictorial description for geographic features than Seoul Commercial Map. Seoul Address Map includes 64 advertisements most of which were hospitals, and securities(banking) located in Junggu and Jongrogu. The Commercial Map includes 67 advertisements, of which are other industrial product such as furniture, food and clothes with higher variety than the other map. Maps on Korea are scattered and housed at other countries and antique bookstores need to be monitored and digitalized for further studies for realistic reconstruction of space of genre de vie.

中國 駝山石窟 佛敎彫刻에 대한 考察

梁銀景 동국대학교 불교미술문화제연구소 1995 佛敎美術硏究 Vol.2 No.-

복강 내 체액의 Cytokine 농도를 이용한 난소암과 양성난소질환의 감별진단

양선문,송경은,서장수,조영래 대한임상병리학회 2000 대한임상병리학회지 Vol.20 No.6

총담관 결찰후 집토끼 소엽사이담관의 미세구조적 연구

이상은,박경호,양남길,안의태,고정식 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

담소관은 담즙의 성분중 수분과 무기전해질의 분비 및 흡수기능을 가지고 있는데, 총담관을 결찰하여 인위적으로 담즙울체를 일으킨 후 담소관상피세포의 미세구조적변화를 알아보기 위하여 본 실험을 시행하였다. 집토끼의 총담관을 결찰하고 1일, 3일, 5일, 7일, 및 14일이 경과된 후 간조직을 떼어 전자현미경관찰을 위한 통상적인 방법에 따라 고정, 탈수, 포매의 과정을 거친 다음 전자현미경관찰용 절편을 만들어 JEM 100CX Ⅱ 투과전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 정상 담소관상피세포는 입방형이며, 내강이 커질수록 원주상으로 변하며, 가끔 전자밀도가 높은 세포가 나타났다. 2. 담소관담상피세포의 자유면은 미세융모가 돌출되어 있으며, 드물게 섬모도 관찰되었다. 세포의 위쪽 측면은 폐쇄띠, 부착띠, 부착반을 가진 연접을 이루었으며, 아래 쪽은 미세주름이 나타났고, 기저면은 기저막으로 둘러 싸여 있었다. 3. 총담관결찰 후 담소관은 그 내강이 확장되면서 상피세포의 미세융모의 수는 줄었으며 팽대되었고, 미세사의 증식이 현저하였다. 4. 총담관결찰 후 담소관상피세포 사이의 부착띠가 더욱 발달되는 경향을 보였다. 5. 총담관결찰 후 담소관상피의 기저막은 부분적인 파괴가 관찰되었다. 이상의 결과에서 미세융모의 감소, 내강의 확장 및 기저막의 부분적인 파괴등은 담즙울체로 인하여 담관내의 압력이 증가하므로 나타나는 담소관상피세포의 형태적 변화라 생각된다. The bile ductule is known to have the function of and the secretion and the reabsorption of the bile juice, especially water and inorganic electrolytes. This experiments was performed to study the ultra sturctural changes of the bile ductule of the rabbit liver after common bile duct ligation. Common bilt duct ligation was performed under ether anesthesia. The rabbits were sacrificed on the 1st, 2nd, 3rd, 5th, 7th and 14th day after the operation. Small blocs of livers were fixed 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome, stained with uranyl acetate- lead citrate, and observed with JEM 100CX II electron microscope. The results were as follows: 1. The cytoplasm of normal bile ductular epithelial cell shows lighter density as compared with that of the of the hepatocyte. Occasional dark cells can be seen between the light ductular cells. 2. On the apical free surface of the normal ductular cells, numerous microvilli project into the lumen, and occasional cilia have been observed. The apical pole of lateral surface exhibits junctional complex, including tight junction, intermediate junction and desmosmes, whereas basal pole have a complex interdigitations. Ductular cell rests on a basal lamina. 3. After the common bile duct ligation, bile ductule showed enlargement of lumen, swelling and reduction of microvilli and proliferation of microfilaments. 4. After the common bile duct ligation, bile ductule showed well developed junctional complex and focally duplicated and thickened basal lamina. From the above results, it was concluded that in the acute cholestasis induced by common bile duct ligation, bile ductular cell shows morphological changes, probably to keep the ductular wall from the increasing intraductular pressure.

지식기반사회와 교육의 과제

조경원,양은주 이화여자대학교 사범대학 교육과학연구소 2001 교육과학연구 Vol.32 No.2

현재 우리는 지식기반사회, 사이버시대, 세계화 등으로 표현되는 인류 문명의 대변혁기를 맞고 있으며, 특히 우리의 학교교육은 그 어느 때보다 심각한 위기를 경험하고 있다. 이러한 대내외적인 도전에 부응하여 우리의 교육을 재검토해야 할 필요성에 입각하여 본 연구는 교육적 관점에서 지식기반사회로의 변화가 요구하는 새로운 교육의 방향과 과제를 명료화하는 것을 목적으로 한다. 이를 위하여 먼저 지식기반사회로의 이행이란 사회경제적 삶의 조건에 있어 어떤 변화를 의미하며, 그러한 변화에 있어 중심적 요소로 되는 지식 개념이란 기존의 지식 이해로부터 어떻게 다른지를 분석한다. 이를 기반으로 지식기반사회에서 요구되는 교육의 구조적 변화를 교육체제와 학교유형상의 변화를 중심으로, 교육의 기능적 변화를 지향하는 목표, 교육내용의 중점, 방법상의 변화 및 교사교육에의 시사점으로 구분하여 논의한다. 이로써 지식기반사회에서는 교육 구조의 유연성과 다양성이 요구되고, 학교교육은 창조적 인간 형성을 목표로 기초능력과 문제해결력에 중점을 두는 교육내용으로 구성하고 학습하는 방법의 학습능력 배양의 과제를 안고 있으며, 이러한 변화의 의식적인 주체로서 교사의 전문가적 지식 기반의 확충이 무엇보다 중요함을 조명한다. We confront the radical change in every aspect of social life, which is expressed in the knowledge-based society, information technology revolution, globalization, etc. Schooling in our society, in particular, is undergoing crisis, which is far more serious than ever before. Meeting the need to re-examine our educational system as challenged from both within and out of our society, this study aims at illuminating the new direction and tasks for the structural and functional transformation of education today. To begin with, we explicate the characteristics change in the socio-economic conditions as embedded in turning toward the knowledge-based society, and we analyze the conception of knowledge and knowing as distinguished from the traditional epistemological notions. As for the structural change, we suggest that educational system become flexible enough to secure the life-long learning of every individual and that the diverse forms of school system be allowed so as to develop various programs which meet the different needs of students and parents. As for the functional change, we propose that creativity, individuality, and cooperation be considered as constituting crucial traits of the ideal of an educated person, that the curricular contents become centered upon the general knowledge as well as upon the procedural knowledge such as creative thinking and problem-solving, that the method of teaching and learning become attentive to forming of learning disposition, learning how-to-learn, so-called meta-cognition. Most of all, it is important to secure that teachers become active and creative agents in educational transformation with their own professional knowledge base re-constructed continuously and shared widely.

Head-Space SPME에 의한 생체시료 중 메스암페타민류 분석(Ⅱ)

최화경,양원경,한은영,최상길,정희선 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-