Adamkiewicz 동맥의 폐색에 의한 척수경색 1례

이광훈,이종걸,이태연,신동익,이성현,이상수,한설희,차상훈 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

안동호 상류 운곡천의 이화학적 수질특성과 식물플랑크톤 군집 특성

이중복,이희무,이건주,박정원,박재충,김동걸,권기석 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

This investigation is about the characteristics of phytoplankton community and physciochemical water quality of specific 6-point the Woon-kog stream system in upsteam of the Andong Lake. DO value was showed over 8.1㎎/L at each site and COD_Mn, BOD, T-N, T-P tend to increase as they stream down and that the existence and dominance of phytoplankton was low and it was difficult to conclude the definite correlation of water quality and phytoplankton community. Finally, it seemed to be desirable that alternatives for pollutional reduction should be made and performed on the basis of the continuous monitoring of the inflow to preserve the Andong Lake.

Shade guide의 형태가 색상 결정에 미치는 영향

박걸,김동준,이시은,황윤찬,오원만,황인남 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.3

This study was conducted in order to assess whether the form of the shade guide affects in deciding the color of the teeth using the shade guide. Eight shade light cured composite resins (Esthet-X, Dentsply, Milford, USA) were used in this study. Shade guides including the model of maxillary central incisors, teeth-form shade guide, doughnut form shade guide, and shade guide with perforated gray shield were prepared with eight shade composite resins and provided the codes randomly. After arranging the models of teeth, 19 dentists working at the clinic of the Dentistry of Chonnam University Hospital and 65 students of college of dentistry, Chonnnam University selected the shade guides corresponding to the color of each tooth on the gray board under the D_(65) standard illuminant. Bl shade showed highest accuracy of about 95% among all shade guides of 3 forms applied to the test and regardless of observer, tooth form shade guide showed the highest accuracy (p < 0.05) , and the doughnut form showed the lowest accuracy (p < 0.05). At the time of deciding on the color of the teeth using the shade guides as a result of above, the forms of the shade guides can affect the accuracy, and it suggests that the development of the diversified forms of shade guides, which may obtain more accurate results, is required. 본 연구에서는 shade guide를 이용하여 치아의 색상을 결정하는데 있어 shade guide의 형태가 미치는 영향을 평가 하기 위해 시행하였다 8가지 서로 다른 색상 code (Al, A2, Bl, B2, B3, C2, C3, D3)를 사용하는 광중합 복합레진 (Esthet-X, Dentsply, USA)을 이용하여 각 색상마다 상악 중절치 모형, 치아형 shade guide, 도넛형 shade guide, 및 회색 shield를 포함하는 shade guide를 제작하고 무작위로 기호를 부여하였다. 제작된 치아 모형을 배열하고 전남 대학교병원 치과 진료처에 근무하는 19명의 수련의와 전남대학교 치과대학 2, 3학년 학생 65명을 대상으로 회색 배경 판과 D_(65) 표준광 하에서 각 치아의 색상과 일치하는 shade guide를 선택하게 하였다. 연구 결과 Bl 색상은 실험에 적용한 3가지 형태의 모든 shade guide에서 약 95%의 가장 높은 정확도를 보였으며 , 색상간 색차가 가장 적은 B2와 C2는 3가지 형태의 모든 shade guide에서 서로 비슷한 정도의 교차 선택율을 보였다. 또한 관찰자에 상관없이 치아 형태의 shade guide는 가장 높은 정확도를 보인 반면 (p < 0.05), 도넛 형태의 shade guide는 가장 낮은 정확도를 보였다 (p <0.05)

Proteomics analysis of salt-induced leaf proteins in two rice germplasms with different salt sensitivity

Lee, Dong-Gi,Woong Park, Kee,Young An, Jae,Geol Sohn, Young,Ki Ha, Jung,Yoon Kim, Hak,Won Bae, Dong,Hee Lee, Kyung,Jun Kang, Nam,Lee, Byung-Hyun,Young Kang, Kyu,Joo Lee, Jeung Canadian Science Publishing 2011 Canadian journal of plant science. Revue canadienn Vol.91 No.2

<P> Lee, D.-G., Park, K. W., An, J. Y., Sohn, Y. G., Ha, J. K., Kim, H. Y., Bae, D. W., Lee, K. H., Kang, N. J., Lee, B.-H., Kang, K.-Y. and Lee, J. J. 2011. Proteomics analysis of salt-induced leaf proteins in two rice germplasms with different salt sensitivity. Can. J. Plant Sci. 91: 337-349. This study was conducted to investigate salt-stress-related physiological responses and proteomics changes in the leaves of two rice (Oryza sativa L.) cultivars. Shoot growth and water content of rice leaves were more severely reduced in Dalseongaengmi-44 than in Dongjin under salt stress. The salt-sensitive Dalseongaengmi-44 exhibited a greater increase in sodium ion accumulation in its leaves than the salt tolerant Dongjin. Comparative analysis of the rice leaf proteins using two-dimensional gel electrophoresis (2-DGE) revealed that a total of 23 proteins were up-regulated under salt stress. Based on matrix-assisted laser desorption ionization-time of flight mass spectrometry and/or electrospray ionization-tandem mass spectrometry analyses, the 23 protein spots were found to represent 16 different proteins. Ten of the identified proteins were previously reported to be salt-responsive proteins, while six, class III peroxidase 29 precursor, beta-1,3-glucanase precursor, OSJNBa0086A10.7 (putative transcription factor), putative chaperon 21 precursor, Rubisco activase small isoform precursor and drought-induced S-like ribonuclease, were novel salt-induced proteins. Under salt stress, fragmentation was increased in several proteins containing the Rubisco large chain. The results of these physiological and proteomics analyses provide useful information that can lead to a better understanding of the molecular basis of salt-stress responses in rice. </P>

Studies on the skin microbiome and solid state fermentation for cosmetic materials

Dong-Geol LEE,Jeoungjin RYU,Minji KIM,Misun KIM,Hyeonju YEO,Seunghyun KANG 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Comparative analysis of human facial skin microbiome between topical sites compared to entire face

Lee Haeun,Jeong Jinuk,Oh Yunseok,Lee Cherl-Jun,Mun Seyoung,Lee Dong-Geol,Jo HyungWoo,Heo Young Mok,Baek Chaeyun,Heo Chan Yeong,Kang So Min,한규동 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Skin is an essential outer barrier and supports the growth of commensal microorganisms that protects a host from the ofense of foreign toxic organisms. With the rapid development of next-generation sequencing (NGS)-based applications, skin microbiome research for facial health care has reached industry growth, such as therapy and cosmetic product development. Despite the acceleration of skin microbiome research, experimental standardization protocol has not yet been established in the facial site and method of sampling. Objective Thus, we aimed to investigate the diferences in microbial composition at each facial site (cheek, mouth, forehead, and entire face) using comprehensive microbiome analysis. Methods Twelve specimens from three men (four specimens per one person) were collected. The hypervariable regions (V3–V4) of the bacterial 16S rRNA gene were targeted for 16S amplicon library construction and classifcation of bacterial taxonomy. Skin microbial composition for all specimens was investigated, and the diferences site-by-site in skin microbial composition were analyzed and evaluated by the various statistical tests. Results We were able to validate the independent correlation between the skin microbiome composition and the facial sites. The cheek site showed the highest alpha-diversity in richness and evenness scores compared to the forehead and mouth. The cheek and mouth sites showed a positive correlation (R2 value>0.93) with the entire face, while the forehead sites were negatively correlated (R2 value<0.2). Given the relative abundance based on statistical correlation analysis, we estimated that the cheek site could be considered an optimal topical site to replace the entire face. Conclusion Our study suggests that skin microbiome profling of four facial sites confrms that the cheek shows the most similar skin fora with the entire face. This study would be informative for preventing bias caused by sampling methods before researching and understanding skin cosmetics development or skin diseases.

Zobellella maritima sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment

Lee, Dong Wan,Lee, Hanbyul,Kwon, Bong-Oh,Khim, Jong Seong,Yim, Un Hyuk,Park, Hongjae,Park, Byeonghyeok,Choi, In-Geol,Kim, Beom Seok,Kim, Jae-Jin Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.7

Comparative whole genome analysis of face-derived Streptococcus infantis CX-4 unravels the functions related to skin barrier

Lee Haeun,Lee Dong-Geol,Jo HyungWoo,Heo Young Mok,Baek Chaeyun,Kim Hye-Been,COSMAX BTI,Kang Seunghyun,Lee Wooseok,Mun Seyoung,Han Kyudong 한국유전학회 2024 Genes & Genomics Vol.46 No.4

Background The skin microbiome is essential in guarding against harmful pathogens and responding to environmental changes by generating substances useful in the cosmetic and pharmaceutical industries. Among these microorganisms, Streptococcus is a bacterial species identified in various isolation sources. In 2021, a strain of Streptococcus infantis, CX-4, was identified from facial skin and found to be linked to skin structure and elasticity. As the skin-derived strain differs from other S. infantis strains, which are usually of oral origin, it emphasizes the significance of bacterial variation by the environment. Objective This study aims to explore the unique characteristics of the CX-4 compared to seven oral-derived Streptococcus strains based on the Whole-Genome Sequencing data, focusing on its potential role in skin health and its possible application in cosmetic strategies. Methods The genome of the CX-4 strain was constructed using PacBio Sequencing, with the assembly performed using the SMRT protocol. Comparative whole-genome analysis was then performed with seven closely related strains, utilizing web-based tools like PATRIC, OrthoVenn3, and EggNOG-mapper, for various analyses, including protein association analysis using STRING. Results Our analysis unveiled a substantial number of Clusters of Orthologous Groups in diverse functional categories in CX-4, among which sphingosine kinase (SphK) emerged as a unique product, exclusively present in the CX-4 strain. SphK is a critical enzyme in the sphingolipid metabolic pathway, generating sphingosine-1-phosphate. The study also brought potential associations with isoprene formation and retinoic acid synthesis, the latter being a metabolite of vitamin A, renowned for its crucial function in promoting skin cell growth, differentiation, and maintaining of skin barrier integrity. These findings collectively suggest the potential of the CX-4 strain in enhancing of skin barrier functionality. Conclusion Our research underscores the potential of the skin-derived S. infantis CX-4 strain by revealing unique bacterial compounds and their potential roles on human skin. Background The skin microbiome is essential in guarding against harmful pathogens and responding to environmental changes by generating substances useful in the cosmetic and pharmaceutical industries. Among these microorganisms, Streptococcus is a bacterial species identified in various isolation sources. In 2021, a strain of Streptococcus infantis, CX-4, was identified from facial skin and found to be linked to skin structure and elasticity. As the skin-derived strain differs from other S. infantis strains, which are usually of oral origin, it emphasizes the significance of bacterial variation by the environment. Objective This study aims to explore the unique characteristics of the CX-4 compared to seven oral-derived Streptococcus strains based on the Whole-Genome Sequencing data, focusing on its potential role in skin health and its possible application in cosmetic strategies. Methods The genome of the CX-4 strain was constructed using PacBio Sequencing, with the assembly performed using the SMRT protocol. Comparative whole-genome analysis was then performed with seven closely related strains, utilizing web-based tools like PATRIC, OrthoVenn3, and EggNOG-mapper, for various analyses, including protein association analysis using STRING. Results Our analysis unveiled a substantial number of Clusters of Orthologous Groups in diverse functional categories in CX-4, among which sphingosine kinase (SphK) emerged as a unique product, exclusively present in the CX-4 strain. SphK is a critical enzyme in the sphingolipid metabolic pathway, generating sphingosine-1-phosphate. The study also brought potential associations with isoprene formation and retinoic acid synthesis, the latter being a metabolite of vitamin A, renowned for its crucial function in promoting skin cell growth, differentiation, and maintaining of skin barrier integrity. These findings collectively suggest the potential of the CX-4 strain in enhancing of skin barrier functionality. Conclusion Our research underscores the potential of the skin-derived S. infantis CX-4 strain by revealing unique bacterial compounds and their potential roles on human skin.

A 1,1′-biuracil from Epidermidibacterium keratini EPI-7 shows anti-aging effects on human dermal fibroblasts

Dong-Geol LEE,Yeong-Geun LEE,Misun KIM,Minji KIM,Chaeyun BAEK,HyungWoo JO,Hyeonju YEO,Nam-In BAEK,Seunghyun KANG 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.10

Oceanimonas marisflavi sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium

Lee, Dong Wan,Lee, Hanbyul,Kwon, Bong-Oh,Khim, Jong Seong,Yim, Un Hyuk,Park, Hongjae,Park, Byeonghyeok,Choi, In-Geol,Kim, Beom Seok,Kim, Jae-Jin Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.9