A Basic Study on Leaf and Stem Production of Angelica acutiloba

Choi,Seong-Kyu,Yun,Kyeong-won,Chon,Sang-Uk,Lee,Jong-Ill,Seo,Young-Nam,Seo,Kyoung-Sun,Choi,Kyeong-Ju 한국자원식물학회 2004 Plant Resources Vol.7 No.3

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by using connect pots in 2003 and 2004. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than those harvested in 2003. Germination rate of Angelica acutiloba seeds collected in 2003 was 10%, while germination rate of seeds collected in 2004 was above 90%. Especially, plant growth and yield of Angelica acutiloba grown in connect pots sized with 4×4×5cm(length×width×height) were the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by using connect pots and optimizing seed collecting time in greenhouse.

생리활성물질을 생성하는 해양미생물의 동정 : Ⅰ. 항미생물 물질을 생산하는 해양방선균 분리균주 No.101의 분리 및 배양조건 Ⅰ. Isolation and Cultural Conditions of the Marine Actinomycetes No.101 Producing Antimicrobial compounds

최종덕,박욱연 國立統營水産專門大學 附設 水産科學硏究所 1994 수산과학연구소보고 Vol.5 No.-

본 연구에서는 생리활성물질을 생성하는 해양미생물을 분리하기 위하여 연근해역의 해면을 조사하였다. 해면 중의 미생물은 1.2×10~2.1X10^3/g 범위에 있었고, 이들 중 방선균은 0.Ol~O.5%(data 생략)로 나타났다. 분리된 미생물은 각각 배양하여 항균물질을 검토하였으며, 검토하던 중에 비교적 강한 항균물질을 생산하는 균주 No. 101을 분리, 선발하였다. 선발된 균의 특징은 sclerotia로 형태가 다양하였고, 다양한 배지에서 생육이 가능하였고, 기균사의 색깔은 회백색, 배면의 색깔은 주로 회백색에서 갈색으로 나타났으며, 젤라틴 액화능과 전분 분해능을 가지나 myo-inositol은 이용하지 못하였다. 이 균의 배양학적 특성은 최적 pH 및 온도가 6.5~7.0 및 30℃였으며, 탄소원으로는 starch가 30g/l, 질소원으로는 casamino acid 10g/l가 적합한 것으로 나타났다. 방선균 No. 101의 균체 및 균배양액의 추출물에 대한 항균 및 항진균성에서는 Staphyloccus aureus와 Bacillus subtilis에서 강한 항균력을, 효모와 곰팡이에 대하여는 낮은 항진균성을 나타내었다. Marine organisms were investigated to identify the marine actinomycetes that produced noble bioactive compounds. Microorganism counts range from 2.1×10^3 to 1.2×10 CFU/g of marine organisms. Actinomycetes constituted 0.01 to 0.5% of culturable microbial community. We identified the marine actinomycetes that produced novel bioactive compounds. During the course of screening for bioactives from the marine microorganisms, we found that the strain in sponge had antimicrobial activities. From the morphological, cultural and various physiological characteristics, this strain was identified for Actinomycetes No. 101. The optimal compositions of culture medium for Actinomycetes No. 101 were starch 30g/l as carbon source, casamino acid l0g/l as nitrogen source. The optimal pH of medium and fermentation temperature were 6.5~7.0 and 30℃, respectively. Fermentation has been conducted in the marine broth at 30℃ for 72 hour. The yield of fermentation got about 3g as dry weight(per liter of broth). The distribution of antimicrobial activity of Actinomycetes No. 101 was screened by paper disc. The extract of cultured cell and broth inhibited the growth of Staphylococcus aureus and Bacillus subtilis, but the inhibition action was week against yeast and mold.

제조업체 생산관리상의 문제점에 관한 비교 연구

최응순,곽종욱 관동대학교 기업경영연구소 1999 경영논집 Vol.18 No.-

이온성 액체를 함유한 고분자 전해질의 전기화학적 특성

최재원,김진규,김종욱,안주현 한국공업화학회 2004 응용화학 Vol.8 No.2

Ionic liquids have being investigated for many applications including reaction solvent, separation medium, and electrolytes of supercapacitor and solar cell. Since ionic liquids are liquids which are entirely composed of ions, they can have high ionic conductivity. They are also non-volatile and non-flammable, and exist as liquid state over a wide range of temperature compared with organic solvents commercially used. Therefore, when they can be used as electrolytes, it can make more safe battery than commercial one. In this study, a series of ionic liquids was synthesized and ionic liquids were soaked into microporous P(VdF-HFP) membrane, the polymer electrolytes were also prepared. Microporous membrane was prepared by phase inversion method. Their thermal stability was preformed by TGA and their electrochemical properties were evaluated with ionic conductivity, electrochemical stability and cyclic voltammogram.

소음성난청 유소견자들의 사후관리 실태

최장선,송재석,원종욱,강종두,차봉석,노재훈 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

The number of workers with noise induced hearing loss which comprise 56.9% in the total number of workers with work-related diseases in Korea of 1994, are becoming in position of the most serious problem. Periodic health examination system which was introduced for health care of workers on hazardous workplace has played important role in health care and the prevention of work-related diseases. The goal of periodic health examination may be obtained when we not only discover occupational disease, but also control them effectively. To study the post-examination health status of workers with noise induced hearing loss diagnosed from 1991 to 1994 in the periodic health examination, all 154 workers in 102 manufacturing factories in Banwol and Siwha industrial complex were reviewed. 1. Among 154 workers, 87 workers(56.5%) were changed to different department, 67 workers(43.5%) remained in the same department. 2. Among 102 workplaces, only 23 workplaces(22.9%) had changed their working environment while 79 workplaces(77.1%) did not. 3. In this study, post-examination health care of the workers diagnosed as noise induced hearing loss had significant correlated with the presence of health care manager, Industrial Health and Safety Committee in workplace and size of industry. The improvement of working environment is also closely related with the presence of health care manager in workplace. 4. Among 154 respondents, 3 workers(1.9%) had received compensation while 151 workers(98.1%) did not. 5. The reasons for not requesting the compensation were that 117 workers(77.5%) were ignorant of the administrative procedure for compensation, 30 workers(19.9%) were not sure of receiving compensation, 4 workers(2.6%) were busy. Considering the result of this study, we must establish the guide of management or contents of post management for noise induced hearing loss. To prevent the noise induced hearing loss, and protect the workers with noise induced hearing loss, education about post management is much required for employer and employee.

NaCl에 대한 콩 품종간의 생리학적 반응 차이

박종환,천상욱,최원열,정선요 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.2

염분조건에서 콩의 피해정도 또는 피해과정 등 생리적 특성과 내성 및 감수성 품종간의 반응차이를 구명하여 내염성 연구의 기초자료로 활용하고자 본 연구를 수행하였다. 감수성 및 내성 품종으로 선발된 두 품종간에 60mM의 염분처리에 따른 생장 및 상대생장율 반응, 잎의 해부학적 관찰, 광합성 및 호흡량, proline 축적 및 nitrate reductase와 peroxidase 활성 등의 반응특성을 비교한 바 다음과 같은 결과를 얻었다. 내성품종 Lee는 무염처리에 비해 초장이 19% 감소한데 반해 감수성 품종 Jackson은 40% 감소하여 뚜렷한 차이를 보였다. 다육질의 정도는 내성품종이 무처리에 비해 58% 증가하였으나 감수성 품종은 오히려 21%가 감소하였고 감수성 품종의 생체중, 건물증, 수분함량의 감소가 뚜렷하게 나타났다. 상대생장율(RGR)과 순동화율(NAR)의 경우 내성품종은 염분처리에 의해 오히려 증가한 경향이었으나 총 건물증에 대한 엽면적 비율(LAR)과 개체생장율(CGR)은 감소하였다. 한편 감수성 품종은 조사된 모든 생장율이 감소하는 경향이 뚜렷하게 나타났다. 염분처리로 잎의 두께는 내성품종이 11% 증가한 반면 감수성 품종은 30% 정도 감소되었는데 이는 다즙질화로 인한 엽육세포의 두께 변화와 관련이 있음을 알 수 있었다. 광합성 정도는 내성품종에서 무처리와 유사하였으나 감수성 품종은 무처리에 비해 14% 감소하였고 호흡량에 있어서는 두 품종 모두 증가되어 각각 11과 8% 증가하는 것으로 나타났으며 두 품종 모두 O2생산비율보다 O2소모비율이 높게 나타났다. 염분처리에 따른 proline 축적량은 무처리에 대비하여 내성품종은 64% 증가하였으나 감수성 품종은 200% 증가하였다. Nitrate reductase의 활성은 염분처리로 인해 내성 품종이 114% 증가하는 반면 감수성 품종은 반대로 37% 감소하였으며 peroxidae 활성은 내서 및 감수성 품종 모두 각각 56, 50% 증가하는 경향을 보였다. 이상의 결과로 보아 지상부의 생육, 상대생장율, 엽육세포의 두께, 광합성 및 호흡량, proline 축적량, nitrate reductase와 peroxidase활성은 콩의 염해 및 내염성 연구에 중요한 지표가 될 수 있음을 확인할 수 있었다. Salt tolerance of soybean is highly dependent on cultivar as well as cultural condition. The objective of this study was to determine difference in parameters of physiological responses between NaCl-tolerant and NaCl-susceptible cultivars selected from a bioassay. Tolerant cultivar "Lee" and susceptible cultivar 'Jackson' soybean (Glycine max (L.) Merr.) cultivars were grown in hydroponic system and treated with NaCl solutions of 0 and 60mM, corresponding to electric conductivity of 1.2 and 7.3 dS/m, respectively, 20days after emergence. NaCl at 60mM inhibited plant lengths of tolerant and susceptible soybean cultivars by 19% and 40%, respectively. NaCl enhanced leaf succulence of tolerant cultivar by 58%. while it decreased that of susceptible cultivar by 21%. With NaCl treatment, fresh and dry weight, and water content of leaves in susceptible cultivar were more decreased than those of tolerant cultivar. Relative growth rate (RGR) and net assimilation rate (NAR) of tolerant cultivar were increased by NaCl treatment, while RGR and NAR of susceptible cultivar were clearly reduced. Total leaf thickness of tolerant soybean cultivar was significantly enhanced up to 11% by NaCl treatment due to expanding mesophyll cells. However. NaCl reduced leaf thickness of susceptible cultivar by 30 8. Photosynthesis rate of tolerant cultivar by NaCl treatment was not affected, however that of susceptible cultivar was reduced by 14% as compared to control. Respiration of both cultivars was increased up to 11 and 8%, respectively. Free-proline content of leaf by NaCl treatment was increased by 64% in the tolerant cultivar and 200% in the susceptible. respectively. NaCl of 60 mM also increased nitrate reductase and peroxidase activities of tolerant cultivar by 114% and 56%. respectively, while it reduced nitrate reductase activity of susceptible cultivar by 37%. The results suggested that the physiological data can use as parameters for salt stress or salt tolerance test to improve sensitivity in screening program.

林産油脂樹種의 利用에 관한 硏究(Ⅱ) : 비목나무 種實의 成分分析 Analysis of Component in Lindera erythrocarpa Seed

趙鍾洙,崔相旭 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

林産油脂의 資原活用을 위한 비목나무 種實에 대한 脂質 및 아미노酸의 分析 結果를 要約하면 다음과 같다. 미목나무種實의 化學的 組成은 組脂肪이 67.36%, 灰分이 2.50%이였으며 物理-化學的 組成에서는 比種이 0.962, 屈折率이 1.4691, 酸價가 9.63, 비누化價가 133.6, 요오드價가 67.6으로 나타났다. 그리고 總脂肪酸 組成에서는 myristoleic acid, lauric acid, caprid acid가 대체로 높게 나타났으며 脂質의 構成을 보면 中性과 糖脂質에서는 myristoleic acid, lauric acid가 높은 함량을 보여 전체적으로는 總脂質과 같은 경향을 보였다. 試料의 構成아미노酸 分析에서는 必順아미노酸을 포함하여 17種으로 構成되어 있었으며 이中 glutamic acid가 27.26%로 가장 높게 나타났고 다음이 aspartic acid가 14.47%의 含量을 보였으며 proline는 存在하지 않았다. We investigated the composition of chemical components of Lindera erythrocarpa seed. The chermical compositions of Lindera erythrocarpa seed consisted of 4.50% of moisture, 67.36% of crude fat, 2.50% of ash. The physico-chemical characteristics of the seed oil were determined as follows: specific gravity, refractive index, acid value, sapomification value and iodine value were 0.926, 1.4691, 9.63, 133.6, and 67.8, respectively. The lipids in the crude oil were almost composed of neutral lipids. Also it contained all essential fatty acids. The seed oil contained 12 different kinds of fatty acids which were myristoleic acid, lauric acid, oleic acid, capric acid, palmitoeic acid, linoleic acid, palmitic acid, lauroleic acid. eicosanoic acid, stearic acid , linolenic acid and myristic acid. The content of myristoleic acid in total lipid, neutral lipid and glycolipid is the highest than that of other lipids. The seed contained 17 different kinds of amino acids such as glutamic acid, aspartic acid, leucine, arginine, valine and so on. The content of glutamic acid is the highest than that of other amino acids.

임신중 우측난소의 자궁내막장종의 성장과 파열의 1례

이종학,홍순도,남상욱,최종호,허광현,박일영 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.2

계명의대 산부인과에서 35세된 경산부에서 임신과 동반된 우측 난소의 자궁내막낭종의 성장과 파열을 경험하였기에 이에 문헌고찰과 함께 보고하는 바이다 Pregnancy had long been considered to have beneficial effect on endometriosis. During the pregnancy, extrauterine endomertrial tissues did not growth or enlarged because of physiologic amenorrhea and producing hormonal effect. However, we experienced a patient who underwent emergency exploratory laparotmy at gestation week 34/4 for rupture of an ovarian endometriotic cyst. The patient had a Cesarean section delivery of a 1,960gm female baby, who died at 1st day of life.

社會體育政策 方向과 活性化 方案 硏究

朴鐘旭,崔榮旭 대불대학교 1995 論文集 Vol.1 No.1

The establishment of goal for the purpose of practising efficient sports for all Should be considered as follows : 1. The sound View for the Physical education and the sports activity should be established. 2. Considered the feature and balance between the local community, the Sufficient facilities for the sports for all should be Secured. 3. The organization for the development and distribution of the program for the sports for all Should be established at the national wide. 4. The college graduates majoring in the sports for all should be given a certificate for the authorized second class leader and the college graduates specializing the physical education or the people completing the training course for the leaders of the sports for all passing the examination should be given a certificate for authorized third class leader. 5. The proper fund should be Secured through the investment of enterprises, the governmental budget, the lottery tickets and local tax for the promotion of sports for all. 6. The training institute for the executives who take charge of sports for all should be established and the mutual operation and support system between the government and local communities should be archived. 7. The Variety of mass media and the governmental support should be used to make the social atmosphere for the living physical education.

자운천 유역의 강우특성에 의한 하천 탁도 발생에 관한 연구

김종욱,박수진,최한규 江原大學校 産業技術硏究所 2007 産業技術硏究 Vol.27 No.B

This research investigates the turbidity and flow of river for an year, 2005, in order to examine the relation of the generation of turbidity according to the outflow of min water. For this research, the region of Jawoon river where is the area of high land vegetable growing in the upper Soyang Reservoir is selected to observe actual floating materials that generate negative nutrition and turbidity of the Reservoir water and the changes of water quality by raining of each month, In addition, the researcher has conducted statistical inspection methods, such as correlation analysis and regression analysis on strength of raining force, and rain continuance time among the elements affecting the outflow of floating materials.