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박욱연,박영범,최근표 도립 강원전문대학 1999 道立 江原專門大學 論文集 Vol.2 No.-
This study was performed to investigate the biological activity-anticancer activity, thrombolytic activity, hypoglycemic activity-of Chicorium intybus and it was extracted with hot water, and then hot water extract was further fractionated into hexane and water. The anticancer effect of Chicorium intybus extracts against cancer cell line, human lung cancer(A549), human hepatocellular carcinoma(Hep3B), human normal lung cell(HEL299) was investigated. The growth of A549 inhibited over 50% all sample which concentration was 1.0g/L and Hep3B inhibited over 40% too. The thrombolytic activity enhanced more 2.33 times in hexane fraction that compared lysis zone (0.9cm) of control, plasmin 10unit. In hypoglycemic activity test, enzyme activity of α-glucosidase which mainly cause diabetes were most inhibited by hexane fraction and inhibition ratio in adding the water extracts, hexane fraction and aqueous fraction were scored 24.5%, 32.1%, 29.5%, respectively.
박욱연,김신희,김지회,김용관,장동석 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.4
The ethanol extract from the root bark of Morus alba showed the strongest antimicrobial activity on the growth of almost all the tested microorganisms which were food-borne pathogens and food-related microorganisms. 1) In order to isolate and purify of antimicrobial substance extracted from the root bark of Morus alba, the antimicrobial substance from the ethanol extract which exhibited a strong antimicrobial activity was purified by solvent fractionation, silica gel column chromatography, TLC and HPLC. Among the fractions fractionated by 4 kind of solvents from the ethanol extract, the antimicrobial activity of ethyl acetate fraction had the strongest antimicrobial activity against B. subtilis. Unknown compound were isolated from the ethyl acetate fraction by silica gel column chromatography, TLC and HPLC and the compounds showed strong absorbance at 207, 217 and 285 nm, therefore, it was supposed to be a kinds of aromatic compound.
박욱연 도립 강원전문대학 1999 道立 江原專門大學 論文集 Vol.2 No.-
In order to develop a natural food preservative, root bark of Morus alba was extracted with ethanol and then bactericidal activity of the extract against microorganism was investigated. The stability for temperature, pH, storage conditions and metal ions of the extracted antimicrobial substance was investigated. The crude fat content of the extract from root bark of Morus alba was 62.9% and palmitic acid and stearic acid were pretty high in fatty acid composition. The extract from root bark of Morus alba had bactericidal activity against Gram positive bacteria such as Bacillus subtilis, and Staphylococcus aureus. While the extract had bacteriostatic activity against Gram negative bacteria such as Escherichia coli. The minimum bactericidal concentration(MBC) of the extract against B. subtilis and S. aureus was 32㎍/㎎ and 64㎍/㎎, respectively. The antimicrobial activity of the extract was stable by the change of pH, freezing and heat treatment. KCl, NaCl and MgCl_(2) didnot affect bactericidal activity of the extract from root bark of Morus alba but CaCl_(2) did negative affect bactericidal activity.
박욱연,김영목,김신희,장동석 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.3
In order to develop a natural food preservative, the root bark of Morus alba was extracted with several solvents, and then antimicrobial activity was investigated. The optimum extracting condition for the antimicrobial substance from the sample, minimum inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. The antimicrobial activity of the ethanol extract from the sample was atronger than those of the extracts by the other solvents such as water, methanol, ethyl acetate and acetone. The optimum extracting condition for antimicrobial substance from the sample was shaking extraction twice for 5 hours at room temperature in case of 7 times of absolute ethanol added to the crushed root bark of Morus albs. The ethanol extract from the root bark of Mores albs had strong antimicrobial activity against Gram positive bacteria(MIC, 6.4-19.2 g/ml) such as B. subtilis, B. cereus, L. monocytogenes and S. aureus. Especially, Bacillus species was the most susceptible to the extracted substance. The ethanol extract showed antimicrobial activity against Gram negative bacteria(MIC, 160-1600 g/ml) and yeasts(MIC, 1600 g/ml) such as C. albicans and S. acidifaeciens. The extract also showed growth inhibition against molds such as A. niger, A. parasiticus, A. versicolar and T. uiride.
박욱연,박영범 강원도립대학 2000 道立 江原專門大學 論文集 Vol.3 No.-
Antimicrobial activity of ethanol extract from Cinnamomi Cortex (Cinnamomum cassia Blume) against food-borne pathogens and food-related microorganisms was investigated using a paper disk method. The results indicated that ethanol extract from Cinnamomi Cortex had antifungal activity against Aspergillus oryzae and Candida albicans at 210.5 and 421㎍ per disk, respectively. This ethanol extract from Cinnamomi Cortex was fractionated by solvents such as n-hexane, chloroform, ethyl acetate, n-butanol, and water. Among these fractions, n-hexane fraction showed the highest antifungal activity against fungi such as A. oryzae at 61.5㎍ per disk. The n-hexane fraction was further purified by silica gel and flash column chromatography. The strong antifungal activity was found in the fraction 2 and compound I at 7.24 and 3.1㎍ per disk, respectively. The Purified compound I was analyzed and compared with authentic specimens by ^(1)H-NMR spectroscopy. On the basis of ^(1)H-NMR spectral data, compound I was identified as tracts-cinnamaldehyde.
상백피 추출물이 미생물의 균체성분 및 형태 변화에 미치는 영향
박욱연,성희경,목종수,장동석 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.3
The ethanol extract from the root bark of Mores alba showed the strongest antimicrobial activity on the growth of almost all the tested microorganisms which were foodborne pathogens d food-related microorganiama. Therefore, fatty acid composition, amino acid composition and shape change of microorganisms treated with the ethanol extract from the root bark of Mores alba were examined. In effects of treatment with the ethanol extract on the fatty acid compositions of B. aubtilis, S. aureus and E. coli, fatty aicd compositions such as hexadecanoic acid (16:0) and octadecanoic acid (18:0)/octadecadienoic acid (18:2) of the tested strains were increased but pentadecanoic acid (15:0), heptadecanoic acid (17:0) and octadecenoic acid (18:1) of E. subtilis, pentadecanoic acid (15:0) of S. ctureus and hexadecenoic acid (16:1) and octadecenoic acid (18:1) of E. coli were decreased. The ethanol extract did not significantly affect the amino acid composition of the tested strains. Tranamisaion electron micrographa of microorganisms treated with the ethanol extract exhibited morphological changes that irregularly contracted cell surface in S. aureus and destructed cell walls in B. subtilis and E. coli.