1989년 10월 12일 충남 홍성에서 발생한 토네이도

최부섭(Boo-Sub Choi),이언구(Eon-Gu Lee),홍성길(Sung-Gil Hong) 한국기상학회 1990 Asia-Pacific Journal of Atmospheric Sciences Vol.26 No.1

백서 구개 전층 창상의 치유에 미치는 피부린 접착제의 영향

소광섭,최홍란 전남대학교 치과대학 1998 전남치대논문집 Vol.10 No.1

The prupose of this study was to examine the effects of the fibrin adhesive (Beriplast??) on healing of full-thickness wounds in the rat's hard palate. Twenty Spraque-Dawley strain white male rats, each weighing 250-300 gm were used. Creation of full-thickness wounds of 4 × 4 mm in size were performed on the hard palate. Beriplast??, a wound dressing material , was applied immediately in the experimental group, but not applied in the control group. All wounds were protected with palatal resin splints. The animals were sacrificed on the 2nd, 4th, 7th, 14th, and 28th day after the operation for macroscopic and microscopic examinations. Results obtained were as follows ; 1. On the 7th day after the operation, epithelial proliferation was greater in the experimental group than that in the control group. 2. The inflammatory reaction of the experimental group was less than the control group on the 2nd and 4th day after the operation. Beriplast?? was resorbed on the 7th day after the operation. 3. In the control group, the epithelial proliferation occurred from the 7th to the 14th day after the operation, and in the experimental group, epithelial proliferation occurred from the 4th day after the operation. 4. On the 14th and 28th day after the operation, there was no prominent difference between the two groups in histological findings. These results suggest that the use of fibrin adhesive (Beriplast??) as a palatal wound dressing results in greater epithelial proliferation and less inflammation in the early stage of wound healing.

인공호에서의 강수와 저수율에 따른 수질변화

이종영,최홍섭,장봉기 순천향대학교 기초과학연구소 1996 순천향자연과학연구 논문집 Vol.2 No.1

수도 전용 댐으로 사용하고 있는 인공호인 가창 댐에서 강수와 저수율에 따른 수질 변화를 조사 분석하기 위하여 1993년 1월부터 1994년 7월까지 정기적으로 매일 취수하여 실험한 상수 원수의 이화학적 수질 성적과 강수와 저수율의 자료로서 관련성을 분석한 결과를 요약하면 다음과 같다. 강수 유무에 따라 탁도, 용존산소, 전기전도도, pH는 통계적으로 유의한 차이를 보였다. 저수율 변화에 따라서는 탁도, KMnO₄소비량, 용존산소, 알칼리도, 전기전도도등 pH 각 항목모두에서 유의한 차이가 있었다. (p〈0.01). 이화학적 수질 항목은 계절에 따라 영향을 받는 것으로 나타났다. (p〈0.01). 탁도는 여름에 가장 높았고 KMnO₄소비량은 가을에 높았으며, 용존산소는 여름에 낮았다. 알칼리도는 가을에 낮았고, 전기전도도는 여름에 높았으며, pH는 여름에 낮게 나타났다. 이화학적 수질 항목에 대한 다변량분석 결과 탁도, KMnO₄소비량, 용존산소, 알칼리도, 전기전도도, pH 각 항목에 있어 강수는 유의한 변수가 아니었고 저수율과 계절은 수질 항목 모두에서 유의한 변수로 나타났다. (p〈0.01). 이상의 결과로 인공호에서의 수질은 계절에 따라 발생하는 자연적인 영향과 저수율에 의해서 크게 변함을 알 수 있었다. 따라서 인공호에서의 취수하는 상수 원수의 수질을 보다 최적으로 유지하는데는 저수율의 효과적인 관리 계획에 중점을 두어야 할 것이다. This study was conducted to examine the changes in water quality by seasons, existence of rainfall and rate of storage at Kachang Dam which has been used only for the piped water service. The biochemical water quality had been daily sampled and tested from January 1993 to July 1994. The result of this study is summarized as follows. Turbidity (p〈0.01), dissolved oxygen (p〈0.01), electrotransmittance (p〈0.05) and pH (p〈0.01) were significantly changed, but KMnO₄consumption and alkalinity were not significantly changed by existence of rainfall. All of tested items for water quality, such as turbidity, KMnO₄consumption, dissolved oxygen, alkalinity, electrotransmittance and pH were showed significant difference in the rate of storage at reservoir (p〈0.01). The biochemical water quality was influenced by seasons (p〈0.01). The highest turbidity was determined in summer, KMnO₄consumption was determined high in autumn, but dissolved oxygen and pH were lower in summer, alkalinity was determined low in autumn, electrotransmittance was determined high in summer. In multiple analysis on water quality items, the rainfall was not significant variable in turbidity, KMnO₄consumption, dissolved oxygen, alkalinity, electrotransmittance and pH , but the rate of storage, season were showed significant variable all items for the water quality (p〈0.01). As a result of testing, it was found that the change of water quality was influenced by the rate of storage at reservoir. It is required, therefore, to emphasize the effective control planning of the rate of storage at reservoir, so as to keep water quality collected from reservoir in the best condition.

Conjoined Audio Fingerprint based on Interhash and Intra hash Algorithms

Kim, Dae-Jin,Choi, Hong-Sub The Korea Contents Association 2015 International Journal of Contents Vol.11 No.4

In practice, the most important performance parameters for music information retrieval (MIR) service are robustness of fingerprint in real noise environments and recognition accuracy when the obtained query clips are matched with the an entry in the database. To satisfy these conditions, we proposed a conjoined fingerprint algorithm for use in massive MIR service. The conjoined fingerprint scheme uses interhash and intrahash algorithms to produce a robust fingerprint scheme in real noise environments. Because the interhash and intrahash algorithms are masked in the predominant pitch estimation, a compact fingerprint can be produced through their relationship. Experimental performance comparison results showed that our algorithms were superior to existing algorithms, i.e., the sub-mask and Philips algorithms, in real noise environments.

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

구개열에서 비인두강의 생리해부학적 구조와 과비음과의 연관성 연구

조준희,표화영,최홍식,최병재,손흥규,심현섭 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

비인강폐쇄란 연구개, 인두측벽 그리고 인두후벽간의 움직임이 서로 조화되어 구강과 비강을 나누어주는 괄약근 기전으로서 연하, 호흡, 발음 등의 생리적기능에 중요한 역할을 한다. 이 기능에 문제가 생긴 경우를 비인강폐쇄부전이라하며 그 원인으로는 (1) 연구개의 길이 및 움직임이상 (2) 비인두강의 해부학적 공간문제 (3) 인두 후벽과 측벽의 기능이상 등이 있다. 본 연구는 구개열 환자의 측면두부방사선계측사진을 통해 비인두강을 생리해부학적으로 분석하였으며 산출된 말소리의 과비음정도를 Nasometer로 평가하였다. 이로부터 얻은 정상군과 구개열환자군의 결과를 각각 비교하였으며, 비인강폐쇄부전과의 연관성을 알아보기 위하여 Anatomic VPI와 Nasalance score의 값을 비교분석하였다. 얻어진 결과는 다음과 같았다. 1. 측면두부방사선계측사진 결과, 연구개 길이, 연구개 두께, 비인강 깊이, 비인강 면적, Adequate ratio에서 두 그룹간 유의한 차이를 나타내었다. 2. Nasometer 결과. 모음/오/와 구강공명음문장, 구강장해음문장에서 두 그룹 간 유의한 차이를 나타내었다. 3. 구개열환자군에서 비인두강의 폐쇄부전 정도를 표현해주는 Anatomic VPI와 Nasalance score는 전반적으로 연관성이 없었다. 다만, 모음/이/와 일부 구강자음으로 이루어진 문장에서 다소의 상관성을 나타내었다. 결론적으로, 측면두부방사선계측사진과 Nasometer 각각의 검사결과에서 두 그룹간 유의한 차이를 찾아볼 수 있었으나, 구개열환자군내에서 비인강폐쇄부전을 표현하는 Anatomic VPI와 Nasalance score는 모음/이/와 구강자음을 포함한 문장을 제외하고는 전반적으로 연관성이 없었다. Velopharyngeal closure is a sphincter mechanism between the activities of the soft palate, lateral pharyngeal wall and the posterior pharyngeal wall, which divides the oral and nasal cavity. It participates in physiological activities such as swallowing, breathing and speech. It is called a velopharyngeal dysfunction when this mechanism malfunctions. The causes of this dysfunction are defects in (1) length, function, posture of the soft palate, (2) depth and width of the nasopharynx and (3) activity of the posterior and lateral pharyngeal wall. The purposes of this study are to analyze the nasopharynx of cleft palate patients using cephalometry and to evaluate the degree of hypernasality using nasometry to find its relationship with velopharyngeal dysfunction. The following results were obtained : 1. In cephalometry, there were significant differences in soft palate length, soft palate thickness, nasopharyngeal depth, nasopharyngeal area, and adequate ratio between two groups. 2. In nasometry, there were significant differences between two groups in vowel /o/ and sentences including oral consonants. 3. In cleft palate patients, though no general correlation was found between Anatomic VPI and nasalance scores, vowel /i/ and sentences including oral consonants were slightly correlated. In conclusion, cephalometry and nasometer results were significantly different between the two groups. Though in the cleft palate group. Anatomic VPI and nasalance scores, which are indices for velopharyngeal closure, excluding the vowel /i/ and sentences including oral consonants show generally no significance.

세로토닌성 항우울제가 백서의 Schedule-Induced Polydipsia에 미치는 영향

이기철,이정호,박중섭,최영민,전성일,정홍경,하준명,정재현 대한생물치료정신의학회 1999 생물치료정신의학 Vol.5 No.2

Object : Schedule-induced polydipsia is considered as an animal model of obsessive-compulsive disorder inrats. The authors evaluated the chronic effects of fluoxetine and clomipramine as serotonergic antidepressants and haloperidol as dopaminergic antagonist on the schedule-induced polydipsia in rat.Methods : Spraque-Dawley rats weighing 200-250gm were individually housed, maintained and allowed free access to water for 1 week. And then the rats were placed on a restricted diet. To induce polydipsia, rats were placed in automatic cage where a pellet dispenser automatically dispensed 90mg pellets on a fixed-time 60 seconds(FT 60s) feeding schedule over 150-minute test session for a day. Water was available at all times during the feeding schedule in automatic cage. After 4 weeks of daily exposure to the FT 60s feeding schedule, experimental rats met a predetermined criterion for polydipsic behavior(greater than 3 times of water per session on average). 4 groups of rats were administered fluoxetine(5mg/kg/i.p.), clomipramine(5mg/kg/i.p.), haloperidol(0.1mg/kg/i.p.), vehicle(1cc/kg/i.p.) for 3 weeks. Rats were tested once a week to access schedule induced polydipsic behavior. The chronic effects of experimental drugs on schedule induced polydipsic behavior were analyzed with repeated analysis of variance and Scheffe test as a post-hoc comparison.In order to measure water consumption in non-polydipsic food-deprived rats, a separate group of rats(N=8) were individually housed and given a single bolus(14.5 gm) of food per day which maintained them at their average body weight.Results and Conclusion : The results were as follows ;1) After 4 weeks of daily feeding procedure with fixed time schedule for 60 seconds per day, the experimental group showed significant differences than the control in the amount of water consumption as compared with their baseline water intakes. At the same periods, there were no differences between the experimental group and the control in body weight. 2) The clomipramine treated group and the fluoxetine treated group showed significant decrease in the amount of water intake as compared with their baseline of polydipsic water intakes for 3 weeks of treatment. However, the haloperidol treated group and the vehicle control group showed no changes of amounts of water intake for 3 weeks of treatment as compared with their baseline of polydipsic water intakes. 3) At 2 weeks of drug treatment, clmipramine treated group(16.88±6.51ml) and the fluoxetine treated group(22.50±10.35ml) showed significantly lower amounts of water intake than the haloperidol treated group (41.25±7.06ml) or vehicle control group(37.50±12.54ml). And also the clomipramine treated group(13.75±5.18ml) and the fluoxetine treated group(18.75±3.54ml) showed significantly lower amounts of water intake than the haloperidol group(35.00±11.65ml) and the vehicle control(34.38±6.78ml) at 3 weeks of drug treatment. Above findings suggest that the fixed time feeding procedure for schedule-induced polydipsia as an animal model of obsessive compulsive disorder was effective to the evaluation of pharmacological challenge study. The author confirmed that schedule-induced polydipsia was successfully decreased for 3 weeks of administration of clomipramine and fluoxetine but there was no response to haloperidol.

Characterization of OsJAC1 which is responding to different types of ionizing radiation

In jung Jung,Jung Eun Hwang,Sung Min Han,Hong-Il Choi,Soon-Jae Kwon,Jin-Baek Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Ionizing radiation affects gene expression from plant genomes. To monitor the genome-wide transcriptional changes induced by three types of ionizing radiation, we used the rice RNA sequencing to identify genes that are up- or down-regulated by gamma rays (GAs), proton (PRs) and ion beams (IBs). The Oryza sativa jacalin-like lectin domain containing proteins (OsJAC1) gene was highly induced by GAs, PRs and IBs. OsJAC1 was selected based on the expression patterns of a genome-wide dataset of RNA sequencing. Many jacalin-related lectin genes have been shown to be associated with disease resistance, biotic and abiotic stress signaling. Therefore, we studied its expression pattern in response to different abiotic stress and phytohormone treatments. The expression patterns of OsJAC1 under two different abiotic stress conditions (salt and heat stress) and phytohormones (salicylic acid and methyl jasmonate) were examined. The transcripts of OsJAC1 were significantly induced in response to abiotic stress conditions, including salt and heat treatments. In addition, it was induced in response to the salicylic acid and methyl jasmonate treatments, respectively. To investigate the sub-cellular localization of OsJAC1, the gene was expressed as a fusion protein tagged with GFP, in tobacco leaf epidermis and examined under confocal microscope. The OsJAC1 was clearly localized at the nucleus. These results provide critical insights into the molecular functions of the rice jacalin-like lectin domain containing proteins as receptors of external signals.

Role of hemoglobin in hemoglobin-based remediation of the crude oil-contaminated soil

Hong, Jin-Kyung,Jho, Eun Hea,Choi, Hyo Sub,Kang, Guyoung Elsevier 2018 Science of the Total Environment Vol.627 No.-

<P><B>Abstract</B></P> <P>This study investigated the changes in the indigenous microbial community structure with hemoglobin (Hb) application to determine the role of Hb in Hb-based remediation of crude oil-contaminated soil. The phylogenetic diversity of the bacterial community showed that the Hb addition selected surfactants-producing species, thereby, promoting TPH degradation. The significant increase in the CO<SUB>2</SUB> generation, which can be related to the increase in the bacterial abundance inferred from the 16S rRNA gene copy number, supports the enhanced TPH degradation with Hb application. The similar residual TPH concentrations in the presence of only hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) and both Hb and H<SUB>2</SUB>O<SUB>2</SUB> suggested that the role of Hb as a catalyst was not as significant as the role of Hb as a nutrient. Also, in the presence of H<SUB>2</SUB>O<SUB>2</SUB>, a greater recovery of the microbial community structure was observed with the double Hb injection than the single Hb injection. Overall, this study shows that the Hb-based remediation strategies via microbial metabolism can be successfully applied to remediate the crude-oil contaminated Kuwaiti soil.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hemoglobin (Hb) imposed a selective force on surfactant-producing bacterial species. </LI> <LI> Hb application promoted TPH removal in the aged crude oil-contaminated soil. </LI> <LI> Hb-based biocatalytic reaction removed TPH from the crude oil-contaminated soil. </LI> <LI> Bacterial community structure recovered better after the biocatalytic reaction. </LI> <LI> Hb-based remediation can be a viable option for crude oil-contaminated soils. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

율무근의 식물화학적 성분 연구 및 Tyrosinase 저해 활성

최윤혁 ( Yun-hyeok Choi ),최춘환 ( Chun Whan Choi ),이재연 ( Jae Yeon Lee ),안은경 ( Eun-kyung Ahn ),오좌섭 ( Joa Sub Oh ),홍성수 ( Seong Su Hong ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.1

본 연구는 천연 미백소재 탐색을 위하여 율무(C. lachryma-jobivar. ma-yuen) 부산물 추출물의 tyrosinase 저해활성을 검증하였으며, 그 중에서 뿌리 추출물은 159.3 μg/mL의 IC50 값을 나타내었다. 율무 뿌리 추출물의 순차적 용매 분획에 대한 활성 검증 후 가장 활성이 우수한 EtOAc 분획물에 대하여 DiaionHP-20 column chromatography, MPLC및 preparative HPLC를 수행해 율무근으로부터 여섯 개의 화합물을 정제하였고, 이 물질들의 구조 분석은 LC-MS와 NMR 데이터 해석을 바탕으로 진행하여 각각 (+)-icariol A₂ (1), zhepiresionol (2), 4-hydroxybenzaldehyde(3), trans-ρ-coumaric acid (4), N-(2-hydroxy-4-methoxyphenyl)-2-hydroxyacetamide (5), coixol (6)로 구조를 규명하였다. 화합물 1은 이 식물에서 처음으로 분리되었으며, 화합물 5는 자연계로부터 처음으로 분리된 화합물로 확인하였다. 이들 화합물 중 4-hydroxybenzaldehyde (3), trans-ρ-coumaric acid (4)와 coixol (6)은 순서대로 707.4, 6.5, 62.4 μM의 IC₅₀값을 나타내어 대조군으로 사용한 arbutin (IC₅₀ =618.7 μM)과 비교시 유사하거나 우수한 활성을 확인하였다. 이로 미루어 보아, 율무근 추출물과 활성물질의 tyrosinase 저해활성을 확인할 수 있었으며, 율무근 추출물의 미백소재로서의 적용 가능성을 확인할 수 있었다. In the course of screening tyrosinase inhibitory activity, EtOAc-soluble fraction of Coix lachryma-jobi var. mayuen Stapf. (Gramineae) roots showed significant inhibition. Further fractionation of the EtOAc-soluble fraction resulted in six compounds, which were identified as (+)-icariol A₂ (1), zhepiresionol (2), 4-hydroxybenzaldehyde (3), trans-ρ-coumaric acid (4), N-(2- hydroxy-4-methoxyphenyl)-2-hydroxyacetamide (5), and coixol (6). The chemical structures of these compounds were identified on the basis of spectroscopic methods (MS, 1D and 2D NMR) and comparison with literature values. Compound 1 was first reported from this plant. Also, this is the first time that the isolation of compound 5 has been reported from nature source. Among the isolated compounds, compounds 4 and 6 showed enzyme inhibitory activity, with IC₅₀ values of 6.5 and 62.4 μM, respectively, in comparison with these of positive control, arbutin.