일본 개설서와 사전류의 개항기 기술 분석

현명철(Hyun, Myung?Cheol) 한일관계사학회 2006 한일관계사연구 Vol.25 No.-

사지마 아끼꼬 교수의 제1주제 발표에 대한 논평

玄明哲 인천가톨릭대학교 출판부 1999 누리와 말씀 Vol.- No.6

반복성 혈뇨(IgA 신병증 및 non-IgA 신병증)와 알레르기성 자반증 신염의 면역지표의 변동에 관하여

현명철,고철우,구자훈 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

A prospective study was conducted to see the changes of immune system in recurrent gross hematuria(IgA nephropathy and non-IgA nephropathy) and HSP nephritis in children. Study patients consisted of 60 children with recurrent gross hematuria and Henoch-Scho¨nlein purpura nephritis (8 IgA nephropathy, 24 non-IgA nephropathy and 28 HSP nephritis). The cellular immune indices(total T, T4, T8 cells and T4/T8 ratio) and humoral immune indices (IgG, A, M and E) were measured during the hematuric and non-hematuric period. Following results were obtained. The proportion of T4 cells of peripheral blood during the hematuric period of patients with IgA nephropathy rose to 35.4±14.9% from the non-hematuric value of 20.0±9.1%. The T4/T8 ratio during hematuric period of patients with IgA nephropathy rose to 1.51±0.77 from non-hematuric value of 0.73±0.33. The values of serum IgA and IgE during hematuric period of patients with IgA nephropathy rose to 237±106 ㎎/dl, 231±226 IU/dl from non-hematuric values of 140±10, 28±23, respectively. These changes of cellular and humoral immune indices showed statistically significant differences(p<0.05). However, these changes were not found in patients with non-IgA nephropathy nor HSP nephritis. In conclusion, it can be said that the immune mechanism involved in IgA nephropathy is different from that of HSP nephritis.

제주도산 山草, 海藻 및 버섯類 水溶性 抽出物의 抗癌效果

김수현,오창경,고용구,김성홍,오명철 제주대학교 1994 논문집 Vol.39 No.-

제주지역에서 생산되는 식물 중에서 파리풀(지방명;가신새), 삼지구엽초, 미역, 톳, 표고버섯 및 만년버섯(일명;영지)에서 열수 및 냉수추출물을 얻어 마우스 암모델 세포인 sarcoma-180 세포를 ICR 마우스에 이식하여 고형암 성장저지율과 수명연장율에 대한 항암성을 검색한 결과는 다음과 같다. 산초추출물의 항암효과는 파리풀에서 53.4%, 삼지구엽초에서 58.2%의 고형암 성장저지율을 보였다. 해조류추출물의 항암효과는 톳에서 63.1%, 미역에서 38.2%의 고형암 성장저지율을 보였다. 버섯추출물의 항암효과는 만년버섯에서 62.8%, 표고버섯에서 66.4%의 고형암 성장저지율을 보였다. 산초, 해조, 버섯류 각 추출물에서 복수형암에 대한 효과는 매우 낮았다. 모든 시료는 일정량을 초과 투여한 실험군에서 고형암 성장저지효과가 낮거나 수명이 단축되었다. The hot and cole water extracts were obtained from herbs (Phryma leptostachya and Epimedium koeranum), seaweeds(Hizikia fusiforme and Undaria peterseniana), and mushrooms(Gonoderma luciolum and Lentimus deodes) among which have long been known to have antitumor effects. These extracts were tested on growth inhibition rate(I.R) of solid tumor and prolongation rate(P.R) in animal experiment using ICR mouse with a mouse tumor models sarcoma-180 cell. The results were as follows ; The antitumor effects of herb extracts were 53.4% with Phryma leptostachya and 58.2% with Epimedium koeranum in growth inhibition rate of solid tumor. The antitumor effects of seaweed extracts were 63.1% with Hizikia fusiforme and 38.2% with Undaria peterseniana in growth inhibition rate of solid tumor. The antitumor effects of mushroom extracts were 62.8% with Ganoderma luciolum and 66.4% with Lentinus edodes in growth inhibition rate of solid tumor. The effects on ascite tumor of extracts from herbs, seaseeds and mushrooms were so low that no anititumor effect could be recognized. All the samples showed low growth inhibition rate of solid tumor or short life in over dose groups.

肉類의 焙燒 방법에 따른 N-nitrosamine 含量에 關한 硏究

오명철,오창경,김수현 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

소갈비, 돼지갈비 및 통닭을 가스렌지와 숯불로 焙燒한 후 배소 전후의 N-nitrosamine과 그의 전구물질인 TMAO, TMA, DMA, nitrate 및 nitrite의 함량을 측정하였다. TMAO-N, TMA-N 및 DMA-N은 배소 전후 모든 시험구에서 뚜렷한 변화가 없었으며, 그 함량은 각각 N.D∼0.76 mg/kg, N.D∼2.57mg/kg 및 0.02∼0.60mg/kg 범위였다. 배소 후 nitrate-N는 감소하였고, nitrite-N는 증가하였으며, 그 함량은 각각 4.0∼6.7mg/kg 및 0.7∼3.2mg/kg 범위였다. N-nitrosamine은 배소 후에 NDMA가 5.9∼20.9 ㎍/kg, NDEA가 0∼3.0㎍/kg으로 검출되었다. The contents of N-nitrosmines and its precursors such as TMAO-N, TMA-N, DMA-N, nitrite-N and nitrate-N after broiling of beef-ribs, pork-ribs and chicken upon gas range and charcoal fire were investigated. The contents of TMAO-N, TMA-N and DMA-N no changed in all tested meats before and after broiling, and were in the range of N.D∼0.77mg/kg, N.D∼2.57mg/kg and 0.02∼0.60mg/kg, respectively. Nitrate-N discreased and Nitrite-N increased after broiling, and the contents were in the range of 4.0∼6.7mg/kg and 0.7∼3.2mg/kg, respectively. N-nitrosamines detected NDMA and NDEA in the broiled meats, and the contents were in the range of 5.9∼20.9㎍/kg and 0∼3.0㎍/kg respectively.

자기공명영상을 이용한 만성 족관절통의 원인 분석

김현철,이경태,유재원,이상홍,선재명 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Purpose : Chronic ankle pain is one of the most popular problems with orthopedic clinicians, but it has complex etiologic factors in diagnosis. Recently, magnetic resornance imaging has used as common diagnostic modality in ligaments and tendon problems of ankle sprain. The purpose of this article is to determine the appearances and frequency of chronic ankle sprain by MRI, and to determine the value of MRI as a diagnostic tool. Materials and Methods : We have analysed MRI findings of 84 chronic sprained ankles over 6 months duration from September 1995 to January 1997. All cases had been past history of ankle injury. Among them, sports players ie, soccer, basket ball, competitive runner and ballet dancer are 51 cases( 60.7% ). Average age of patients is 27.06( 13 to 54 ) years old and sex ratio is 23:5. For the confirmative diagnosis, We adopted variable methods, ie, ultrasonogram, bone scan, CT scan, arthroscopic examination and surgical intervention. Results : The Etiologic factors of chronic ankle sprain by MRI includes 39 Lateral ankle instability due to Anterior Talofibular and Calcaneofibular ligamentous tear, 18 Peroneal tendon insufficiency ie, synovitis, longitudinal tear, subluxation and abnormal tendon insertion, 15 Osteochondral fracture of talus or tibia, 10 Anterior Impingement syndrome due to anterior bony spur, 9 Deltoid tear, 6 Os subfibulare, 4 Distal tibiofibular ligament abnormality, 3 Os subtibiale, 2 Ankle synovitis, 2 Intraarticular loose body. Conclusion : MRI study employed in hazy chronic ankle sprain is useful diagnostic modality. But, Diagnostic result using MRI was not correlated the clinical prognosis, and proper physical examination is important in the point of technical aspect to get the exact dissecting angle of MRI.

焙燒 魚類의 N-nitrosamine 含量

김수현,오명철 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

어류 焙燒 후 N-nitrosamine과 그의 전구물질인 TMAO-N, TMA-N, DMA-N, nitrate-N 및 nitrate-N의 함량변화를 조사하였다. 1. TMAO-N은 焙燒 후 어류에서 크게 감소하였으며, TMA-N은 焙燒 후 오징어가 5배, 옥돔이 4배 증가함을 보였다. 2. DMA-N은 배소 후 어류에서 모두 증가하였는데, 이중 오징어가 57.2mg/kg으로 가장 많이 증가하였다. 3. Nitrate-N은 焙燒 후 감소함을 보였는데 그 함량은 2.8∼7.7mg/kg이었다. Nitrite-N은 焙燒 전에 비해 증가하였는데, 그 함량은 2.2∼6.6mg/kg이었다. 4. 어류에서 검출된 N-nitrosamine은 NDMA와 NDEA였다. 焙燒 후 어류의 NDMA는 9. 5∼14.1㎍/㎏, NDEA는 1.2∼2.5㎍/㎏으로 감소하였다. The contents of N-nitrosamines and its precursors such as TMAO-N, TMA-N, DMA-N, nitrite-N and nitrate-N after broiling of sea-bream, Filefish, and Squid upon gas range were investigated. The contents of TMAO-N decreased remarkably in all tested fishes after broiling. TMA-N increased approximately 5 and 4 times in squid and sea-bream, respectively. DMA-N increased in all tested fishes after broiling, and especially showed the highest in squid. Nitrate-N descreased in all tested fishes after broiling, while nitrite-N increased. N-nitrosamines detected in broiled fishes were NDMA and NDEA. The contents of NDMA were in the range of 9.5∼14.1 ㎍/㎏ and NDEA were 1.2∼2.5㎍/㎏.

자연관광지 가로환경물의 색채조화방법에 관한 고찰 : 경기북부지역을 중심으로

최명식,박현철 경희대학교 디자인연구원 2005 예술· 디자인학연구 Vol.8 No.2

This research aims to lay a course for the reformation of the increasingly disorderly and unoriginal designs of environmental objects on the streets of tourist resort and to develop an environmentally friendly color scheme that has a unique identity. In order to do this, the street environments and color schemes of two of northern Gyeonggi-do's most popular tourist spots, the Sanjeong Lake in Pocheon and the Morning Arboretum & Plant Art Center at Kapyeong were sampled and analyzed. Color scheme gauging was done by first taking pictures with a digital camera (Canon EOS D60). Then, using Adobe Photoshop 8.0, the image was taken through a process of Filter/Pixelate/Mosaic (cell size: 10 squares) in order to find the standard color and RGB values of the sampled area, and then composed a 20mm×40mm color scheme pallete based on values. Through this palette it was clear where the color characteristics originated from. The RGB value of the entire palette was Munsell converted in the program CMC Macbeth (Munsell Conversion Version 6.5.1). Through the converted color chart the colors, clarity and brightness could be compared, and the range of colors that would go well with the intended color scheme could be analyzed. A color scheme plan, converted into a NCS Color triangle and color ring according to the principles of color harmony for aesthetic function, was then proposed. Finally the color characteristics of street furniture in environmental areas were analyzed based on this data. The principles of color harmony in street furniture were found and standards were given with this data as a foundation. Additionally, a regional identity based on this color harmony through this kind of color analysis must be established, along with research regarding the materials and styles of street furniture when phasing in these color schemes.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

果采類 殘留性 農藥의 突然變異 誘發性

오명철,오창경,김성홍,고용구,김수현 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

Salmonella typhimurium TA 균주를 이용하여 과채류 재배에 많이 사용되는 12종의 잔류성 농약에 대한 돌연변이 유발성을 검정하였다. 본 연구에 사용된 12종의 농약 중 Tedion, Monopho, Danoton, Ometon 및 Captan에 대한 돌연변이 유발성은 강하였고, Thalonil, Prosing, EPN, Phentoate, Dicopol 및 Captan에 대한 돌연변이 유발성은 중간 정도였으나, Dicopol은 돌연변이 유발성이 없었다. TA98에서, Monopho와 Danoton은 강한 돌연변이 유발성을 나타내었고, Thalonil, Prosing, EPN, Phentoate, Parathion 및 Sappiran은 중간정도의 돌연변이 유발성, Danoton은 약한 돌연변이 유발성을 나타낸 반면, Dicopol과 Sappiran은 돌연변이 유발성을 나타내지 않았다. 한편, TA100에서는 Tedion, Danoton, Ometon 및 Captan이 강한 돌연변이 유발성을, Thalonil과 Monopho는 중간 정도의 돌연변이 유발성, Prosing, Phentoate, Parathion 및 Sappiran은 약한 돌연변이 유발성을 보인 반면, EPN과 Dicopol은 돌연변이 유발성이 없었다. 따라서, EPN은 구조이동성 돌연변이를, Sappiran은 염기치환성 돌연변이를, 그리고 이들과 Dicopol을 제외한 나머지 9종의 농약은 구조이동성 및 염기치환성 돌연변이를 일으키는 돌연변이원임을 확인하였다. Twelve residual pesticides which applied on the fruits and vegetables cultivation were tested for mutagenic activity using Salmonella typhimurium TA strain. Mutagenic activities against Tedion, Monopho, Danoton, Ometon and Captan of twelve pesticides tested were strong, Thalonil, Prosing, EPN, Phentoate, Dicopol and Captan were medium, and Dicopol were no. Tedion, Monopho and Danoton showed strong mutagenic activities on TA98, Thalonil, Prosing, EPN, Phentoate, Parathion and Sappiran showed medium, and Danoton showed weak, while Dicopol and Sappiran showed no. At the other hand, Tedion, Danoton, Ometon and Captan showed strong mutagenic activities on TA100, Thalonil and Monopho showed medium and Prosing, Phentoate, Parathion and Sappiran showed weak, while EPN and Dicopol showed no. Therefore, EPN identified mutagen which induced only frameshift mutation, Sappiran was only base-substitution, and nine except these and Dicopol of twelve pesticides tested were mutagens which induced both frameshift and base-substitution mutation.