다동온실의 복합환경 제어시스템을 위한 소프트웨어 개발

이대원,김채웅 성균관대학교 생명공학연구소 1999 生命工學硏究 Vol.5 No.1

A hybrid environmental control system was designed and built in the multi-greenhouses. After the hardware system was developed, the software was designed and constructed for this project. A software was adequate for reading data from many sensors and operating side windows, top windows, curtains and fans in the two greenhouses. Separate programs for control of the hardware, written Visual basic, Turbo-C and Assembler computer language, were developed to make the hybrid environmental control system operate. The program was a menu driven program within Windows environment. The initial menu was easily implemented by pressing a keypad key(KPK) on the keyboard or a mouse. Continued manipulation of the KPK or a mouse enable the user to reach easily any of the sub modes in the many modes. This software proved to be a reliable program for the hybrid environmental control system in the two greenhouse.

Mossbauer 분광법에 의한 Fe₂SiO₄의 연구

이성호,김웅태,채광표,정덕조 建國大學校基礎科學硏究所 1988 理學論集 Vol.13 No.-

Fe₂SiO₄에 관한 X-선 회절실험과 Mossbauer 분광실험을 하였다. X-선 회절실험 결과 본 시료는 olivine group에 속하는 fayalite로서 orthorhombic 구조(??)임을 알 수 있었다. Mossbauer 분광실험은 60K-834K의 온도구간에서 행하여 졌으며, Mossbaure 스펙트럼을 분석한 결과 철은 high spin 2+ 상태임을 알 수 있었고, X-선 회절실험 결과와는 다르게 철이 산소 이온들과 함께 일그러진 octahedron을 형성하고 있음을 알 수 있었다. The Fe₂SiO₄ has been studied by means of X-ray diffractometry and Mossbauer spectroscopy. The analysis of X-ray diffraction pattern shows that Fe₂SiO₄is fayalite of orthorhombic structure(??). The Mossbauer spectra which have been taken between 60K and 834K show that the valence state of iron is ferrous and high spin state and that the iron ions occupy sites of distorted octahedron whereas the X-ray analysis shows it is at a site of regular one.

골절단을 이용한 급속 치아이동 후 치수 및 치주조직 변화

강경화,김은철,이선경,임채웅,마쓰다 기꾸,태기출,김상철 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.2

본 연구는 골절단을 이용한 치아-치조골 분절의 급속 치아이동 후 치수, 치주인대 및 치조골의 변화를 유성견에서 평가하고자 하였다. 하악 제4소구치의 근심, 원심, 치근단 부위에서 피질골을 절단하여 치아-골 분절을 형성하고 하악 제3소구치를 발거하면서 협측, 설측 부위의 피질골을 삭제하였다. 1주 휴지기 부여에 따라 휴지기군과 비휴지기군으로 나누어 치아의존형 견인장치로 6일 동안 견인하고 강화기를 거친 후 0주, 1주, 2주, 4주, 6주, 8주에 희생시켜 치수, 치주인대 및 치조골의 조직 변화를 임상적, 방사선적, 조직학적 및 면역화학적으로 평가하여 다음과 같은 결과를 얻었다. 1. 치아이동 양과 강화기 동안의 조직 치유 양상에서 휴지기 유무에 따른 차이는 없었다. 2. 견인측에서 골형성은 강화기 8주까지 계속되었는데 강화기 1-2주에 가장 활발하였으며 6-8주간의 변화는 적었다. 3. 비휴지기군의 강화기 1주에서 치조골 흡수 및 파골세포 출현, 염증세포 침윤이 가장 많았으며, 특징적으로 파상아세포가 압박측의 치주인대와 치수 내에 나타났다. 4. TGF-β는 치조골의 골기질 및 골모세포, 파골세포, 치수 내 파상아세포에서 강양성 발현을 보인 반면 치수, 백악모세포, 무세포성 백악질에서 경미한 양성 발현을 나타내는 부위 특이성이 있었다. 5. TGF-β는 견인측 치주인대의 치조골에 인접한 혈관 및 치주인대세포, 골모세포에서 강화기 초기 1-2주에 주로 발현되었으며 6주 이후에는 발현이 크게 감소하였다. The movement of tooth-bone segments by osteotomy can simultaneously shift tooth and surrounding alveolar bone in a relatively short period. The purpose of this study was to evaluate the tissue changes in pulp, periodontal ligament, and alveolar bone in rapid tooth bone movement with osteotomy. The mandibular 3rd premolar of a dog was extracted and cortical bones of the buccal and lingual area were eliminated, and then cortical bones around the mesial and distal area of root, and below the root apex of the mandibular 4th premolar were osteotomized. After a one week latency period, a tooth-borne distraction device was activated for 6 days. And pulp, periodontal ligament and alveolar bone were evaluated clinically, radiologically, histologically and immunohistochemically at 0, 1, 2, 4, 6, 8 weeks of the consolidation period and conclusions were reached as follows. 1. Latency period didn't affect total amount of tooth movement and healing process of tissue during consolidation period. 2. Bone formation continued through 8 weeks of consolidation in distracted side, with a high peak at 1-2 weeks, and the lowest at 6-8 weeks of consolidation. 3. At 1 week of consolidation, alveolar bone resorption, osteoclast appearance and inflammatory cell infiltration were the most active, and dentinoclasts characteristically appeared on the pulp and pressure side of the periodontal ligament. 4. The expression of TGF-β was area-specific, as it was strong-positive at bone matrix, osteoblast, osteoclast of alveolar bone, and dentinoclast inside pulp, but weak in pulp, cementoblast and acellular cementum. 5. The expression of TGF-β was generally observed at the initial 1-2 weeks of consolidation at vessels, periodontal ligament cells, and osteoblast near alveolar bone on the distraction side of the periodontal ligament, and was significantly decreased after 6 weeks of consolidation.

Gene expression of AGS cells stimulated with released proteins by <i>Helicobacter pylori</i>

Kim, Nayoung,Park, Woong-Yang,Kim, Jung Mogg,Park, Ji Hyun,Kim, Joo Sung,Jung, Hyun Chae,Song, In Sung Blackwell Publishing Asia 2008 Journal of gastroenterology and hepatology Vol.23 No.4

<P>Abstract</P><P>Background and Aim: </P><P>Interactions between released proteins by <I>Helicobacter pylori</I> (<I>H. pylori</I>) and the cells of gastric epithelium to which it adheres may contribute to gastric inflammation and epithelial damage. The present study was performed to evaluate the gene expression of AGS gastric cancer cells stimulated with released proteins by <I>H. pylori</I>.</P><P>Methods: </P><P>Gene expression of AGS cells to the stimulation by <I>H. pylori</I>-released proteins (G27 strain) were monitored using oligonucleotide microarrays.</P><P>Results: </P><P>Eighty-eight genes (0.88%) and eight genes (0.08%) were up- or downregulated, respectively, by treating AGS cells with <I>H. pylori</I>-released proteins but not by <I>H. pylori</I> adhesion after 12 h of coculture. Out of the selected 40 up- and five downregulated genes, 29 upregulated genes classified as general RNA polymerase II transcription factor activity (<I>GTF2B</I>, <I>PPARGC1A</I>), <I>SH3</I>/<I>SH2</I> adaptor activity (<I>CRKL</I>), transferase activity (<I>ACLY</I>, <I>CRKL</I>, <I>PIGC</I>, <I>PLK4</I>), and oxidoreductase activity (<I>IDH1</I>) were confirmed to be upregulated by released proteins and not by <I>H. pylori</I> adhesion by real-time reverse transcription–polymerase chain reaction. When the concentrated <I>H. pylori</I>-cultured supernatant prepared by our protocol was treated by boiling, the upregulations of 26 of these 29 genes (89.7%) except for <I>CD160</I>, <I>ZNF268</I>, and <I>PSAT1</I> disappeared. This confirmed that most of these upregulations were caused by released proteins.</P><P>Conclusion: </P><P>Host genes involving transcription, signaling and stress are significantly modulated by the proteins released by <I>H. pylori</I>. This might strengthen the gastroduodenal pathogenesis induced by <I>H. pylori</I>.</P>

Synthesis and luminescence characteristics of conjugated dendrimers with 2,4,6-triaryl-1,3,5-triazine periphery

Kim, Chae Kyu,Song, Eun Seok,Kim, Hyung Joo,Park, Chiyoung,Kim, Young Chul,Kim, Jai Kyeong,Yu, Jae Woong,Kim, Chulhee Wiley Subscription Services, Inc., A Wiley Company 2006 Journal of polymer science Part A, Polymer chemist Vol.44 No.1

<P>We have synthesized conjugated dendrimer with triazine peripheries, and their luminescence properties were investigated. The dendrimers consist of dendritic triazine wedges for electron transport, distyrylbenzene core as an emitting moiety, and t-butyl peripheral groups for good processing properties. The dendrimers have LUMO values of about −2.7 eV possibly because of the triazine moiety with high electron affinity. Photoluminescence study indicates that energy transfer occurs from the triazine wedges to the stilbene bridge, and finally to the core chromophore units due to a cascade decrease of bandgap from the peripheral wedge to core moiety. Therefore, the emission wavelength was determined by the structure of the core unit. The energy transfer efficiency of distyrylbenzene-cored dendrimers was about 75 and 55% for Trz-1GD-DSB and Trz-2GD-DSB, respectively. A preliminary electroluminescence property also was investigated. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 254–263, 2006</P> <B>Graphic Abstract</B> <P>We have synthesized conjugated dendrimer with triazine peripheries, and their luminescence properties were investigated. PL study indicates that the energy transfer occurs from the triazine wedges to the stilbene bridge, and finally to the core chromophore units because of a cascade decrease of bandgap from the peripheral wedge to core moiety. With increasing the generation from the first to the second, the energy transfer efficiency decreased due to the increase of distance between the donor and acceptor. The dendrimers have LUMO values of about −2.7 eV and the EL spectra of dendrimers showed emission maximum at 460 nm. <img src='wiley_img/0887624X-2006-44-1-POLA21142-gra001.gif' alt='wiley_img/0887624X-2006-44-1-POLA21142-gra001'> </P>

DHEA가 실험동물의 면역기능에 미치는 영향

김진호,정혜주,김영옥,정승태,박재현,강선경,김주일,임채웅,김환묵,조대현 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

일반적으로 corticosteroid는 in uivo실험에서 면역파 염증반응을 먹제하는 것으로 알려져 있으나, corticoster oid horBone의 전구체인 DHEA은 in uiuo 및 in oifro실험에서 련역능을 향상시키는 것이 보고된 바 있다. 본 연구에서는 D딘딘A가 정상 실험동물과 면역을 억제시킨 실험동물의 체액성 및 세포매개성 면역능에 미치는 영향을 평가하였다. 7-8 주령의 BALB/C mice에 DHEA를 0.IS, 0.3 및 0.6% 농도로 사료에 혼합하여 2주간 투여한 후 면역계에 미치는 영향을 평가한 결과 비장과 흥선 무게가 모든 투여군에서 감소되었으며 총 비장세포수 또한 감소되었 다. 이들의 비장세포를 분리하석 T, B, CD4+ T및 CD8+ T세포의 분포도를 flow cytometry를 이용하여 측정한 결과 T와 CD4+ T 세포가 모.든 투여군에서 증가되었으나 B 세포는 감소되었고 CD8+ T 세포는 변화가 없었다. 모든 DHEA투여군에서 mitogen (Con A 3! LPS)에 대한 세포분열능이 증가 되었으며 BSA에 차한 항체 형성능을 관찰한 결파 BSA에 대한 IgM과 IgG의 생성능은 모든 투여군에서 감쏘되었다. 2주간 DHEA를 투여한 실험군의 비장세포를 분리하여 in vifro데서 항체생성능을 관찰한 결과 0.15와 0.3% 투여군에서만 항체생성능이 감소되었다. DHEA (0.15, 0.3 & 0.6%)와 동시에 면역억제물질인 dexamethasone (125 uf/animal)을 경구투여하여 dexamethasone에 의하여 야기되는 면역억제에 대한 DHEA의 영향을 평가한 결과, 총 비장세포수는 0.15와 0.3% 투여군에서 증가 하였고, T 세포의 분포도는 0.6% 투여군에서 감소하였으며, B 세포는 DHEA 모든 투여군에서 증가하였다.BSA에 대한 항체 헝성능을 관찰한 결과는 dexamethasone 단독 투여군에 비하따 0.3와 0.6%투여군에서 IgM 항체형성능이 증가 하였고, IgG항체생성능은 0.IS와 0.3%에서 증7'1하였다. 또한 in oifro에서 항체 형성능을 관찰한 결과 모든 D됐EA 투여관에서 증가하였다. IL-2는 0.3% 및 0.6fS의 DHEA 단독투여군과 DH료A와 DSX병용투여군웨선 대조군에 비하여 유의성있게 증가 되었다. IL-4는 0.3% 및 0.6%의 DHEA와 BEX 형용투여군에서 유의성있게 감소되었으며 기타 투여군에세는 유의성있는 변화가 관찰되지 않았다. 이상의 결과로 보아 DHEA는 보편적으로 Thl 형세포의 cytokinf: 분비가 증가되어 세포성 면역반응을 증가시키는 것으로 사료죄며 이러한 효롸는 실험동물의 면역상태에 파라 선택성이 있음을 시사하고 있다. Dehydroepiand·rosterone (DHEA) is a native steroid with an immunomodulating activity that suggested to coullterreguBate seine phenomena of ihlmunosenescence. This study was ca?-ried to evaluate the immunomcaulatory effects of BHEA in normal nlice and/or immunosuppressed micewith dexamethasone (DEX ). DHEA treatment decreased body, spileen and thymus weights, and totagsplenocytes of both normal an,d DEX-imrnunosuppressed mice. In afBdition, DHEA treatment suppressedsenlm antibody titer to bovine serum albumiB and in of'fro productions of Ige and IsM in both groups.However, T tell and B cell responses to the mitogens, concanavalin A and lipopotysaccharide,respectively, and interleukin fIL)-2 production were increased in both groups fellowing DHEA treat-ment. IL-4 sfnthesis by splen()cytes was decreased in immunosupplfessed mice, but did not significantlyalter in normal mice by exposlire to DHEA. In normal mice treated with DHEA were increased splenic Tcells and CO4+ T cells but decreased B cells compared with nontreated mice. The concolnitant treatmentDEX with OHBA iBcreased sphenic B cells but decreased T cells compared with those of DEX only treat-ed mice. These results indicatr that OHEA appears to have modul3.tory effects on the cetl-mediated im-munity rather than humoral immunity in mice.

Poster Session : Expression of a human phenylethanolamine-N-metyltransferase(hPNMT) and design of hPNMT inhibitors using in silico screening and NMR spectroscopy

( Woong Hee Kim ),( Dong Il Kang ),( Jee Young Lee ),( Ji Young Yang ),( Eugene Park ),( Young Kee Chae ),( Yang Mee Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Dietary uptake, biodistribution, and depuration of microplastics in the freshwater diving beetle <i>Cybister japonicus</i>: Effects on predacious behavior

Kim, Shin Woong,Kim, Dokyung,Chae, Yooeun,An, Youn-Joo Elsevier 2018 Environmental pollution Vol.242 No.1

<P><B>Abstract</B></P> <P>Microplastics (MPs) have adverse effects on aquatic organisms in marine environments; however, there is a lack of information on freshwater environments. This study investigated the dietary uptake, and biodistribution and depuration of MPs in the freshwater diving beetle <I>Cybister japonicus</I> (Coleoptera: Dytiscidae) after consumption of zebrafish (<I>Danio rerio</I>) exposed to MPs. The transfer of MPs in diving beetles after consumption of zebrafish was assessed to determine whether the presence of MPs affected diving beetle behavior and predation. We found that diving beetles that consumed MP-exposed fish had a significantly lower ingestion rate than the control. In addition, the trophic transfer rate of MPs was 13–18%. However, MPs were found only in the crop and proventriculus of the beetles, and all particles were depurated within 48 h, likely via regurgitation. As diving beetle is a top predator in freshwater ecosystems and could facilitate transfer from aquatic to terrestrial ecosystems via predation, its behavior towards indigestible MPs in its digestive organs (i.e., filtering and vomiting) could represent a meaningful phenomenon as a potential vector for MP transport. This is the first report of the trophic transfer of MPs from fish to dytiscid species, which helps clarify the effects and mechanisms of MPs in freshwater systems.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microplastic (MP) transfer was assessed between freshwater fish and beetles. </LI> <LI> MPs can be transferred from fish to predacious diving beetles. </LI> <LI> Dietary uptake of MPs affects the ingestion rate of diving beetles. </LI> <LI> Diving beetles can recognize MPs as indigestible food items. </LI> <LI> Diving beetles may transfer MPs from freshwater to terrestrial ecosystems. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Intracellular cleavage of osteopontin by caspase-8 modulates hypoxia/reoxygenation cell death through p53.

Kim, Hyo-Jin,Lee, Ho-June,Jun, Joon-Il,Oh, Yumin,Choi, Seon-Guk,Kim, Hyunjoo,Chung, Chul-Woong,Kim, In-Ki,Park, Il-Sun,Chae, Han-Jung,Kim, Hyung-Ryong,Jung, Yong-Keun National Academy of Sciences 2009 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.106 No.36

<P>Osteopontin (OPN) is highly expressed in cancer patients and plays important roles in many stages of tumor progression, such as anti-apoptosis, proliferation, and metastasis. From functional screening of human cDNA library, we isolated OPN as a caspase-8 substrate that regulates cell death during hypoxia/reoxygenation (Hyp/RO). In vitro cleavage assays demonstrate that OPN is cleaved at Asp-135 and Asp-157 by caspase-8. Cellular cleavage of OPN is observed in apoptotic cells exposed to Hyp/RO among various apoptotic stimuli and its cleavage is blocked by zVAD or IETD caspase inhibitor. Further, over-expression of OPN, the form with secretion signal, inhibits Hyp/RO-induced cell death. Caspase cleavage-defective OPN mutant (OPN D135A/D157A) is more efficient to suppress Hyp/RO-induced cell death than wild-type OPN. OPN D135A/D157A sustains AKT activity to increase cell viability through inhibition of caspase-9 during Hyp/RO. In addition, OPN is highly induced in some tumor cells during Hyp/RO, such as HeLa and Huh-7 cells, which is associated with their resistance to Hyp/RO by sustaining AKT activity. Notably, OPN C-terminal cleavage fragment produced by caspase-8 is detected in the nucleus. Plasmid-encoded expression of OPN C-terminal cleavage fragment increases p53 protein level and induces apoptosis of wild-type mouse embryonic fibroblast cells, but not p53(-/-) mouse embryonic fibroblast cells. These observations suggest that the protective function of OPN during Hyp/RO is inactivated via the proteolytic cleavage by caspase-8 and its cleavage product subsequently induces cell death via p53, postulating caspase-8 as a negative regulator of tumorigenic activity of OPN.</P>

Fabrication and characterization of CGS / CIGS tandem solar cells by low temperature process

Chae-Woong KIM,Hye Jin KIM,Gwangwe YOO,Hyung-Jun KIM,Chaehwan JEONG 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8