벼 유전자원의 저온 발아성에 대한 표현형 및 유전형 특성 평가

현도윤,최유미,이수경,Hyun, Do Yoon,Choi, Yu Mi,Lee, Su Kyeung 국립한국농수산대학교 교육개발센터 2022 현장농업연구지 = Journal of practical agricultural resear Vol.24 No.3

Low-temperature germinability (LTG) is an important trait for the direct seeding of rice in temperate and high altitudes regions. Water temperature of rice fields at sowing season is frequently under 15℃ in Korea, resulting in poor seedlings establishment. The objective of this study was to phenotypically and genetically evaluate 600 rice germplasm composed of six rice subpopulations. In the phenotypic evaluation, aus (AUS) showed the highest visual rating (VR) and germination rate (GR) of 5 and 83.1%, respectively, than other subpopulations. The lowest value of VR (7.1) and GR (53.1%) was observed in aromatic (ARO) subpopulation. Association of the phenotype for LTG with the genotype for qLTG3-1, a major QTL for LTG, and other three SNP markers (LTG_8, qLTG4b-1, qLTG2-6) was conducted. The results suggested that the allele distribution of two markers, qLTG3-1 and qLTG2-6, affects the difference in VR and GR between subpopulations. qLTG4b-1 and LTG_8 were also highly significant with LTG in all subpopulations except that qLTG4b-1 did now show significance in AUS. Therefore, the combination use of these four markers might be effective for evaluation of LTG in rice germplasm.

멜론 유전자원의 형태적 특성 및 유전적 구성 평가

이승범,장익,현도윤,이정로,김성훈,유은애,이수경,조규택,이경준,Lee, Seungbum,Jang, Ik,Hyun, Do Yoon,Lee, Jung-Ro,Kim, Seong-Hoon,Yoo, Eunae,Lee, Sookyeong,Cho, Gyu-Taek,Lee, Kyung Jun 한국작물학회 2020 한국작물학회지 Vol.65 No.4

Melon (Cucumis melo L.), one of the most important fruit crop species, is cultivated worldwide. In this study, a total of 206 melon accessions conserved at the National Agrobiodiversity Center (NAC) in RDA were characterized for nine morphological characteristics according to the NAC descriptor list. In addition, to confirm the genetic composition of each melon accession, genetic profiling was performed using 20 SSR markers. Among the 206 melon accessions, 159 (77.2%) were collected from Asia. The color of fruit flesh and skin were mostly 'white' (56.0%) and 'green' (49%), respectively. Days to female flowering (FD) and maturity (MD) of the accessions ranged from 58 to 72 and 17 to 63, respectively. The fruit length and width of the accessions ranged from 6.0 to 29.3 and 3.6 to 17.2 cm, respectively. The sugar content (SU) ranged from 2.5% to 13.2% with an average of 7.0%. In correlation analysis, SU showed positive and negative correlations with MD and FD, respectively. The accessions were classified into four clusters by cluster analysis. From the results of genetic profiling using 20 SSR markers, three accessions (K189118, K100486, and K190292) were expected to be inbred lines among 206 melon accessions. These results could expand the knowledge of the melon germplasm, providing valuable material for the development of new melon varieties to suit consumer tastes.

방사선을 이용한 내염성 계통의 기내선발 및 특징

이인석,김동섭,현도윤,임용표,이영일,Lee, In-Sok,Kim, Dong-Sub,Hyun, Do-Yoon,Lim, Yong-Pyo,Lee, Young-Il 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4

1.5% NaCl이 함유된 배지에 캘러스를 치상하여 방사선을 처리한 결과 무처리구보다 처리구 (30, 50 Gy)에서 캘러스 생존율 및 재분화율이 증가하여 내염성 캘러스를 선발하는데 방사선의 적용이 효과적임을 알 수 있었고, 내염성 캘러스에서 재분화된 M$_3$세대 종자에서 내염성 계통들은 모품종보다 초장, 근장 및 근수의 생육이 우수하여 이러한 계통은 내염성 연구를 위한 유용한 유전자원으로 이용할 수 있을 것으로 생각된다. 또한 RAPD 기술은 대조구와 내염성 캘러스에서 재분화된 계통을 구분하는데 유용한 기술임을 알 수 있었다. The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.

한국, 불가리아, 엘살바도르 강낭콩(Phaseolus vulgaris L.) 유전자원의 농업 및 종자 형질 비교

이수경(Sookyeong Lee),현도윤(Do Yoon Hyun),이경준(Kyung Jun Lee),이승범(Seungbum Lee),유은애(Eunae Yoo),조규택(Gyu-Taek Cho),고희종(Hee-Jong Koh) 한국육종학회 2022 한국육종학회지 Vol.54 No.2

To compare agronomic and seed traits of 135 common bean landraces originating from Korea, Bulgaria, and El Salvador, we evaluated 20 morphological traits and performed principal component analysis (PCA). In the PCA, the first and second principal components accounted for 55.15% and 15.97% of the total variance, respectively. The first component showed a strong positive correlation with seed size and 100-seed weight, whereas the second component produced a strong negative correlation with days to flowering and days to maturity, indicating that these traits may explain the differences between landraces originating from different countries. Landraces from Korea showed higher variation in days to flowering and days to maturity; those from Bulgaria produced larger and heavier seeds; those from El Salvador produced smaller and lighter seeds and flowering and maturity occurred earlier. In addition, the landraces from El Salvador had relatively lower diversity compared to those from other countries, as they were grouped within a small cluster in the PCA. These evaluation results may provide important information for selecting breeding materials, and diversity analyses of landraces from different countries provide information for securing genetic resources.

국내 밀 43개 품종에 대한 아그로박테리움 형질전환 효율성 검정

김재윤,이건희,이하늘,현도윤,Jae Yoon Kim,Geon Hee Lee,Ha Neul Lee,Do Yoon Hyun 국립한국농수산대학교 교육개발센터 2024 현장농업연구지 = Journal of practical agricultural resear Vol.25 No.4

본 연구는 국내 밀 43 품종에 대한 A. tumefaciens 형질 전환 효율을 검정하기 위해 GUS staining 분석을 수행한 것으로서 대부분의 밀 형질전환 연구가 특정 품종에 국한되어 있기 때문에 국내 장려 밀 품종에 대한 형질전환 효율에 영향을 미치는 조건에 대한 검정이 필요하다. 국내 밀 품종 중 32개에서 1개 이상의 조직에 염색된 신호가 관찰되었으며 4개 품종에서는 염색된 신호가 관찰되지 않았고 6개 품종에서 과도한 A. tumefaciens 성장이 관찰되었고, 7개 품종은 미성숙배의 크기 등의 이유로 GUS staining 분석이 불가능하여 추가 분석에서 제외하였다. 국내 밀 품종별 A. tumefaciens 감염 효율 비교 결과, 백립계 8개 품종 및 적립계 28개 품종에서는 평균적으로 유의한 차이가 없었다. 특히 조농밀, 조품밀, 새올밀, 조중밀, 새금강밀 등 적립계 품종에서 90% 이상의 높은 감염 효율이 관찰되었고, 전체 품종 중 22개는 50% 이상의 효율을 나타내었다. 본 연구를 통하여 조농밀 및 새올밀은 미성숙배에서 전체 조직에서 강한 GUS 발현으로 형질전환에 적합한 품종으로 나타났으며 금강 품종은 백립계에서 상대적으로 높은 발현을 보여 A. tumefaciens을 이용한 형질전환에 적합한 것으로 확인되었다. 추가적인 연구를 통해 품종의 조직배양 효율을 검정하고, 안정적인 GUS 발현 효율을 조사하는 것이 필요하며, 이를 통해 밀 형질전환에 이용 가능한 품종을 더욱 정밀하게 선발할 수 있을 것으로 판단된다. 해당 연구 결과는 국내 밀품종의 형질전환 효율을 높이기 위한 기초 자료로 활용 가능할 것이다. Agrobacterium-mediated transformation (AMT) is a method that allows for the stable integration of DNA fragments into the plant genome. Transgenic plants generated through AMT typically exhibit a lower copy number of the transgene compared to those induced by particle bombardment. Furthermore, AMT offers a straightforward and efficient approach for generating transgenic plants. While the transformation efficiency of wheat is comparatively lower than that of other monocot plants such as Rice (Oryza sativa L.) and Maize (Zea mays L.), the cultivars 'Bobwhites' and 'Fielder' are commonly employed for wheat transformation. To date, there have been no reported instances of successful development of transgenic plants using Korean wheat varieties through AMT. This study aims to assess the transformation efficiency of 43 Korean wheat cultivars using the GUS assay, with the goal of identifying suitable Korean wheat cultivars for AMT. The pCAMBIA1301 vector, carrying the β-glucuronidase (GUS) gene, was incorporated into Agrobacterium strain EH105. Following the inoculation of Agrobacterium into immature embryos, GUS assays were conducted 'Saeol', 'Jopum', and 'Jonong' showed 100% (the number of embryos showing GUS spots/the number of embryos used for AMT) among 43 cultivars. In addition, cultivars with more than 70% were 'Saekeumgang', 'Jojung', 'Tapdong', 'Anbaek', 'Dabun', 'Sugang', 'Keumgang', 'Jeokjung', 'Seodun', 'Joeun', 'Dajung', and 'Baekjung'. It seems that the 15 cultivars above showed the possibility of using AMT. On the other hand, 'Yeonbaek', 'Goso', 'Baekgang', and 'Johan' showed less than 20% and GUS spots were not observed in 'Gru', 'Gobun', 'Milseong', and 'Shinmichal-1'. This study explores transient GUS expression in Korean wheat cultivars seven days after AMT. The observed initial high efficiency of transient transformation suggests the potential for subsequent stable transformation efficiency. Korean wheat cultivars demonstrating elevated transient transformation efficiency could serve as promising candidates for the development of stable transgenic wheat.

방풍, 식방풍의 대사체 프로파일링을 통한 지표성분 선정 및 분석법검증

최보람 ( Bo-ram Choi ),윤다혜 ( Dahye Yoon ),김금숙 ( Geum-soog Kim ),한경숙 ( Kyung-sook Han ),최두진 ( Doo Jin Choi ),이영섭 ( Young-seob Lee ),현도윤 ( Do Yoon Hyun ),이대영 ( Dae Young Lee ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4

방풍(Saposhnikoviae Radix, SR) 과 식방풍(Peucedani Japonici Radix, PR)은 각각 다른 기원식물을 가지며 속과 종이 다르지만 한국, 중국, 및 일본에서 대표적인 전통 한약재로 오랫동안 혼용되어 사용되었다. 본 연구에서는 방풍과 식방풍의 판별 마커를 확인하기 위하여 UPLC-QTOF/MS를 이용한 대사체 프로파일링 및 다변량 통계분석을 진행하였다. 그 결과, 5-Omethylvisammioside와 peucedanol을 각각 방풍과 식방풍의 지표성분으로 선정하였으며, UPLC를 이용하여 분석법을 검증하였다. 제안된 방풍 및 식방풍의 지표성분에 대한 분석법 검증은 방풍과 식방풍의 분류와 품질 평가 및 성분 검증을 위한 효과적인 방법이 될 것으로 기대된다. Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultraperformance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.

동아시아 재래종 밀(Triticum aestivum L.) 유전자원의 고분자 글루테닌 조성과 숙기 특성 평가

이수경(Sukeyung Lee),최유미(Yu-Mi Choi),현도윤(Do yoon Hyun),이명철(Myung-chul Lee),오세종(Sejong Oh),고호철(Hocheol Ko),허온숙(On sook Hur),정연주(Yeonju Jung) 한국육종학회 2015 한국육종학회지 Vol.47 No.3

This study is to raise the utilization of genetic resources of wheat (Triticum aestivum L.) landrace from East Asia (Korea, Japan, and China) by evaluating genetic variation related to end use quality concerned to bread making quality and early maturity, two very important characteristics of Korean wheat cropping system. To clarify the allelic variation of Glu-1 loci which decides HMW glutenin subunit composition, SDS-PAGE and DNA marker analysis were conducted by using 485 East Asian landrace wheat accessions in National Agrobiodiversity Center, RDA and NIAS genebank. And useful accessions getting full mark of glu-1 score and early maturity were selected to enhance the utilization of genetic resources to Korean wheat breeding. In this study, wheats from China showed the distinct characteristic. Whereas Glu-A1c (null) and Glu-B1b (7+8) allele are the most frequent in Korean and Japanese accessions, Glu-A1a (2*) and Glu-B1c (7*+9) are the most in Chinese accessions. When it comes to unique composition, Glu-B1f (13+16) and Glu-D1e (2+10) subunits are only in Chinese resources. Glu-B1d (6+8), Glu-B1e (20), Glu-D1b (7+8), and Glu-D1c (4+1) subunits are only in Korean resources. The accessions from China also has high PIC value (0.53) compared to ones from Korea (0.35) and Japan (0.35). Grouping by UPGMA analysis of combination of Glu-1 allele, most accessions from Korea and Japan are in the same group, but most Chinese ones were distinguished as the distinctive group. The evaluation of bread baking quality by Glu-1 scoring system, 26 accessions got full marks. Among them, 16 accessions from China were also matured before early June, suitable to Korean cropping system. Especially, 3 accessions (K151847, K151865, K151962) had very early maturity, matured in late May. These genetic resources, having good gluten composition and early maturity, are expected to widely be used for Korean wheat breeding.

한국, 중국 및 엘살바도르 강낭콩 (Phaseolus vulgaris L.) 유전자원의 농업 특성과 다양성 비교

최유미(Yu-Mi Choi),이수경(Sugyeong Lee),현도윤(Do yoon Hyun),윤문섭(Munsup Yoon),오세종(Sejong Oh),이명철(Myung-Chul Lee),이정로(Jeongro Lee),고호철(Hocheol Ko),허온숙(Onsook Huh) 한국육종학회 2015 한국육종학회지 Vol.47 No.3

This study was conducted to compare the agricultural characteristics of total 444 common bean accessions from Korea(296), China (76), and El Salvador (72). Days to flowering were ranged from 41 to 83 days with an average of 61 days. Days to sowing to maturing were ranged from 86 to 143 days with an average of 104 days. Common beans from El Salvador tend to bloom and mature 3 to 7 days earlier than Korea and China accessions. In growth habit, over 50% of Korea and China accessions were indeterminate and climbing type, but 90.1% from El Salvador were Semi-determinate and climbing type. Qualitative traits were much different among three countries. Eighty-two percentage of immature pod colors were dark pink from El Salvador, but many of those were pale yellow from Korea (77.6%) and China (61.8%). Seed shapes were divided into four types of round, oval, cubic and kidney type, and the highest percentage of those were 30.4% with kidney type. The highest of those was 36.6% with oval type from Korea, 55.3% with kidney type from China and 79.2% with cubic type from El Salvador. Morphological characteristics of common bean from El Salvador were much different from those of Korea and China, which is necessary to collect more germplasm from its native and expand genetic diversity of common beans. Four hundred thirty-five common beans from Korea, China and El Salvador were analyzed using SSR markers. Ninety-two alleles were detected with a lowest of 6 at the BM161, BM181 and a highest of 18 at the BM154, BM160. The average polymorphism information content(PIC) was 0.72. To similar with population size among three countries, 292 Korean accessions divided four replications with 73 accessions. As a result genetic diversity was the highest of 0.73 in Chinese populations, while the lowest of 0.48 in El Salvador populations.

DAMR-PCR 마커를 이용한 피 ( Echinochloa crus-galli) 유전자원 다양성 분석

송재영 ( Jae Young Song ),박기훈 ( Ki Hun Park ),조광민 ( Kwang Min Cho ),현도윤 ( Do Yoon Hyun ),이수경 ( Sukyeung Lee ),최유미 ( Yu Mi Choi ),박홍재 ( Hong Jae Park ),이석영 ( Sok Young Lee ),이명철 ( Myung Chul Lee ) 전북대학교 농업과학기술연구소 2014 농업생명과학연구 Vol.45 No.2

This study evaluated the effectiveness of directed amplification of minisatellite-region DNA (DAMD) markers in assessing polymorphism and relationships of 69 Echinochloa crus-gali accessions that preserved in the National Agrobiodiversity Center. Using these primers, a total of 113 bands were amplified, of which 65 polymorphic bands were discovered and the percentage of polymorphic bands (PPB) was 57.52%. SPAR profile data were scored for the computation of pairwise distances as well as a Neighbor Joining (NJ) tree of all the genotypes. From the profiles obtained with all the 12 primers considered together, 65 polymorphism bands were scored. The averaged values of gene diversity (HE) and polymorphism information content (PIC) for each DAMD marker were 0.213 and 0.179 within populations, respectively. The results indicate the low levels of the genetic diversity present amongst the genotypes. The 69 Echinochloa crus-galli germplasm accessions were divided into four different groups, and data analysis identified phenetic groups that were in agreement with those obtained according to agronomic traits and DAMD markers. The present results provide evidence of divergence between cultured and wild type barnyard grass. The cultured barnyard grass was seven accessions that had been cultivated in Korea and have been cultivating in India among the 69 Echinochloa crus-gali accessions. The selected seven accessions have thicker culm diameter and bigger seed size than the other groups, and is one of the putative germplasm resources to develop the new forage crop and phytoremediation.

부추 생육불량 원인 구명을 위한 현장조사 결과

조명철(Myeong Cheoul Cho),이주영(Ju Young Lee),곽한강(Han Kang Kwak),김봉환(Bong Han Kim),현도윤(Do Yoon Hyun),최동로(Dong Ro Choi) 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.10