2P-268 A Carbon Dot-Based Fluorescent Nanosensor for Simple Visualization of Bacterial Nucleic Acids

이하늘,류제성,신청,정현정 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.0

실시간 감정인식을 위한 파형 단위 PPG 신호 Labeling 기법

이하늘,강동현,김덕환 한국차세대컴퓨팅학회 2021 한국차세대컴퓨팅학회 학술대회 Vol.2021 No.05

Real-time emotion recognition technology is required for human-robot interaction. There is a method using the Somatic Nervous System (SNS). The PPG signal, which is easy to acquire data and can be used by dividing it into pulse units, is easy for real-time emotion recognition. However, each pulse label is the most important factor in segmenting and using the short-term 1-second to 3-second PPG signal. However, DEAP and MAHNOB-HCI are public bio-signal data, but DEAP Dataset only provides Self-Assessment Labeling for emotion-inducing videos of 60 seconds. The MAHNOB-HCI Database provides Annotation Labeling in which the observer sees the frontal image of the subject's face and measures emotion in real-time. Annotation labeling is effective to split the labels of PPG signals by pulse units. As a result of the experiment, Arousalbased positives 2415/neutral 3884/negative 4201, Valence-based positives 3570/neutral 2835/negative 4095.labels were drived. Annotation labeling allows us to check the emotions of participants in various distributions for 60 seconds. Analyzing the label setting and data preprocessing process contributes to improving short-term real-time emotion recognition.

A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

이하늘,이주희,Yoo Kyung Kang,이주훈,양승주,정현정 한국바이오칩학회 2022 BioChip Journal Vol.16 No.4

We introduce a lateral flow assay (LFA) integrated with a modified isothermal nucleic acid amplification procedure for rapid and simple genetic testing. Padlock probes specific for the target DNA were designed for ligation, followed by rolling circle amplification (RCA) using capture ligand-modified oligonucleotides as primers. After hybridization with detection linker probes, the amplified target DNA is flowed through an LFA membrane strip for binding of gold nanoparticles as the substrate for colorimetric detection. We established and validated the “RCA-LFA” method for detection of mecA, the antibiotic resistance gene for methicillin-resistant Staphylococcus aureus (MRSA). The assay was optimized using various concentrations of primers and probes for RCA and LFA, respectively. The sensitivity was determined by performing RCA-LFA using various amounts of mecA target DNA, showing a detection limit of ~ 1.3 fmol. The specificity of the assay was examined using target DNAs for other resistance genes as the controls, which demonstrated positive detection signals only for mecA DNA, when added either individually or in combinations with the control targets. Furthermore, applying the RCA-LFA method using specifically designed probes for RNA-dependent RNA polymerase (RdRp) and receptor binding domain (RBD) gene for SARS-CoV-2, which demonstrated feasibility of the method for viral gene targets. The current method suggests a useful platform which can be universally applied for various nucleic acid targets, allowing rapid and sensitive diagnosis at point-of-care.

수생태계에서 ZnO, TiO2나노입자 응집체가 물벼룩(Daphnia magna)에 미치는 영향

이하늘,이병우,박찬일,김무찬 해양환경안전학회 2014 해양환경안전학회지 Vol.20 No.5

본 연구에서는 정제되지 않은 ZnO 및 TiO₂나노입자를 M4배지에 노출시켜 두 나노입자가 어느 정도 크기의 응집체로 변화되는지를 살펴보고 또한 두 나노입자가 수생태계 생물종인 Daphnia magna에 어떠한 영향을 초래하는지 유영저해 및 폐사율을 통해 살펴보았다. ZnO 및 TiO₂나노입자의 분말상태 크기는 각각 20 nm와 40 nm였지만, M4배지에서는 1333 nm와 1628 nm로 약 40~70배의 크기로 응집되었다. 유영저해의 경우 ZnO와 TiO₂나노입자 모두 시간 및 농도가 높아질수록 D.magna가 유영하는데 영향을 미친 것으로 나타났으며, 특히 ZnO나노입자가 TiO₂나노입자에 비해 더 큰 영향을 미치는 것으로 나타났다. 폐사율의 경우 ZnO나노입자에서는 시간 및 농도가 높아질수록 폐사되는 비율이 높았으며, TiO₂나노입자에서는 72시간이 경과된 시점의 10 ppm 이상의 농도에서 폐사하는 것으로 관찰되었다. 이는 나노입자가 해양에 유입됨으로 인해 원래의 크기에 비해 응집되어 증가되어진다는 것을 알 수 있으며, 또한 그 응집체로 인해 수생태계 생물에 영향을 주는 것으로 나타났다. This study is unrefined ZnO, TiO2 nanoparticles is expose M4 medium to search nanoparticle aggregation and Daphnia magna was any effect by immobilization and mortality. ZnO and TiO₂ nanoparticle powder-size is respectively 20 nm and 40 nm. but, M4 medium has about respectively as 1333 nm and 1628 nm, 40 to 70 times were agglomerated. Immobilization of ZnO and TiO₂ nanoparticles was influenced both time and concentration the higher to swimming of D.magna. Especially, The immobilization of D.magna in nano-ZnO is greater than that influence in nano-TiO₂. Mortality of ZnO nanoparticle is higher rate at long time and high concentration. TiO₂ nanoparticle observed mortality at 10ppm concentration after 72h. Consequently, when Nanoparticles is introduced into ocean. Particle size become grow. Additionally, aggregation be caused affect aquatic ecosystems.

A Miniaturized Plasmon Detector using 0.25 µm CMOS Process for Large-Scale Sub-Terahertz Imaging

이하늘,윤준혁,양종렬 한국과학기술원 반도체설계교육센터 2023 IDEC Journal of Integrated Circuits and Systems Vol.9 No.1

A miniaturized complementary metal-oxide-semiconductor (CMOS) detector circuit is presented for large-scale 0.2 THz imaging. The proposed detector consists of an integrated differential antenna, common-source (CS) Plasmon detector cores, a buffer stage including pre-amplifiers, and off-chip main amplifiers. The folded dipole structure is used for reducing the unit size of the detector area and supplying the gate bias of the differential CS detector cores. The converted DC outputs at the detector are combined in the drain of the preamplifier as currents and transmitted to the output of the buffer. The off-chip main amplifier, implemented using discrete components, provides a high voltage gain for increasing responsivity and band-pass frequency response for optimizing the electrical modulation in the source. The proposed detector, except for the main amplifiers, is implemented in the area of 500 µm × 370 µm by using 0.25 µm CMOS technology. The responsivity and noise equivalent power of the proposed detector circuit, including the amplifiers, at 0.2 THz were measured to be 2.57 MV/W and 43.61 pW/√Hz, respectively.

타액분비저하에 따른 구강작열감증후군 환자의 임상적 특징 비교

이하늘,김동윤,백소영,정해인,이현진,조윤재,하나연,김진성 대한한방내과학회 2020 大韓韓方內科學會誌 Vol.41 No.5

Objectives: The aim of this study was to investigate the clinical characteristics of patients with Burning Mouth Syndrome (BMS) due to hyposalivation (HS). Methods: We reviewed the clinical records of 39 BMS patients who visited the Department of Digestive Diseases of Kyunghee Korean Medicine Hospital from March 1st, 2020 to July 31st, 2020. The subjects were classified according to the presence or absence of hyposalivation and both groups were compared for the proportion of coated tongue, heart rate variability (HRV), Ryodoraku, and the numeral rating scale (NRS) score of tongue pain results. Results: The BMS with Hyposalivation (HS group) and the BMS without Hyposalivation (Non-HS group) showed a significant difference in the proportion of coated tongue and the NRS score for tongue pain. The NRS score was significantly higher in the Non-HS group and the proportion of coated tongue was lower. However, no significant differences were noted in several HRV parameters between the two groups. The most frequent accompanying symptoms were xerostomia and dyspepsia. Conclusions: The results of this study suggest that hyposalivation might be one of the main causes of tongue pain, the key complaint in BMS patients. Sympathetic/parasympathetic imbalance might not be a main contribution of hyposalivation in BMS. Instead, factors such as the number of medications taken seem to correlate with hyposalivation in BMS. This results could be useful in the management of BMS patients with hyposalivation in clinical practice.

A case of bilateral renal infarct due to spontaneous renal artery dissection

이하늘,민지원,하명아,송호철 대한내과학회 2021 대한내과학회 추계학술대회 Vol.2021 No.1

Combination of vegetable soup and glucan demonstrates synergistic effects on macrophage-mediated immune responses

이하늘,최주희,박지연,안재훈,장다은,심재군,박종환,김영민 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.4

Vegetable soup (VS), a plant-based functionalfood, has been used as a traditional folk medicine and isattracting attention for its ability to enhance the immuneresponse. b-Glucan, a well-established and effectiveimmunomodulator, has synergistic effects when used incombination with some bioactive compounds. In the presentstudy, we aimed to evaluate the synergisticimmunomodulatory effects of the combination of VS andb-glucan on macrophage-mediated immune responses. b-Glucan was demonstrated to synergistically enhance theVS-stimulated immune response, including the productionof interleukin-6, tumor necrosis factor-a, and nitric oxide,mainly through the mitogen-activated protein kinasepathway in macrophages. In addition, this combination hasthe potential for further development in functional foodswith immune-enhancing activity.

가정전문간호사의 비암성 말기환자 간호행위 시간 분석

이하늘,이종은 한국가정간호학회 2019 가정간호학회지 Vol.26 No.2

Purpose: To investigate the duration of each nursing activity performed by home care nurses for non-cancer patients and the relationship between patients’ palliative prognostic index (PPI) and duration of each nursing activity. Methods: Nursing activities performed for six non-cancer terminal patients were timed using a stopwatch, and 18 parameters were measured by visiting each patient thrice. The mean and standard deviations of duration for each category of nursing activities were computed. The relationship between category-specific duration of nursing activities and PPI was analyzed with Spearman’s correlation and multiple regression analysis. Results: Among nursing activities, the highest greatest duration of time was spent on traffic time (11.91 min), followed by urinary catheter management (10.65 min) and insertion and management of nasogastric tube (9.03 min). In terms of nursing categories, after excluding movement time, the greatest duration of time was spent on excretion care (5.48 min), nutrition care (5.40 min), and medication (3.82 min). PPI correlated with hygiene care, excretion care, and patient and information management. Multiple regression analysis revealed that PPI increased with increasing duration of hygiene care. Conclusion: These study findings provide grounds for the increased nursing time of hygiene care for people reaching the end of life.

간절한 하루하루가 모여 결과를 만든다

이하늘 회경사 2018 月刊 會計 Vol.26 No.2