Induced Pluripotent Stem Cells: Next Generation Stem Cells to Clinical Applications

이승범,차지현,박선후 한양대학교 의과대학 2015 Hanyang Medical Reviews Vol.35 No.4

Induced pluripotent stem cells (iPSC) are specially manipulated cells from somatic cells by the introduction of four factors that are reprogrammed. The properties of iPSC are similar to embryonic stem cells (ESC) characteristic of self-renewal and pluripotency. The technology of reprogramming somatic cells to iPSC enables the generation of patient-specific cells that can be used as powerful tools for drug screening, in vitro models for human disease and autologous transplantation. The iPSC technology provides a priceless resource for regenerative medicine but there are still changing obstacles over the safety of iPSC in avoiding induction of tumorigenicity and maintaining high purity of re-differentiated cells from iPSC to produce more functional cells for cell therapy. A variety of methods to overcome the limitation of iPSC application applied in the clinical setting have been developed. In this review, we summarize the recent progress in iPSC generation and differentiation techniques to facilitate clinical application of iPSC with future potential in regenerative medicine.

중·고강도 유산소 운동이 인슐린저항성, 면역기능 및 염증성 사이토카인에 미치는 영향

이승범 한국웰니스학회 2014 한국웰니스학회지 Vol.9 No.2

본 연구의 목적은 16주간의 중·고강도 유산소 운동에 따른 인슐린저항성, 면역기능 및 염증성 사이토카인의 변화에 미치는 영향을 알아보기 위해 실시하였다. 본 연구의 대상자는 50∼55세의 남성으로 정상 집단(n=10)과 비만과 인슐린저항성 집단(n=10) 총 20명으로 선정하였다. 실험기간은 총 16주이고, 주 3회, 1회 운동시간은 40∼50분이며, 측정항목으로 인슐린저항성(Glucose, Insulin, HOMA-IR), 체지방율, 면역기능(IgA, IgG, IgM), 염증성 사이토카인(IL-6, TNF-α, CRP)등을 측정하였다. 자료처리방법은 반 복측정 이원변량분석(two-way ANOVA with repeated measure)을 이용하였으며, 다음과 같은 결론을 도출하였다. 첫째, 글루코스, 인슐린, HOMA-IR은 0주에서 8주(중강도 운동), 8주에서 16주(고강도 운동)에서 유의하게 감소하였다. 둘째, 체지방율, BMI은 0주에 서 8주(중강도 운동), 0주에서 16주(중강도+고강도 운동)에서 유의한 감소를 보였다. 셋째, IgA는 0주에서 8주(중강도 운동)와 0주에 서 16주(중강도+고강도 운동)로 유의한 증가를 보였으며, 8주에서 16주(고강도 운동) 감소한 수치를 보였다. IgG는 큰 차이를 볼 수 없었으나, IgM의 경우 0주에서 16주(중강도+고강도 운동)와 8주에서 16주(고강도 운동)로 유의한 증가를 보였다. 넷째, IL-6와 TNF-α는 0주에서 8주(중강도 운동), 8주에서 16주(고강도 운동)에서 유의하게 감소하였다. CRP는 0주에서 8주(중강도 운동), 0주 에서 16주(중강도+고강도 운동)에서 유의한 감소를 보였다. The purpose of this study is to identify middle-high intensity aerobic exercise on insulin resistance, immune fuction, inflammatory cytokine. The subjects were totally 20(n=10 in the normal group, n=10 in the obesity-insulin resistance group). The subjects was provided with 3 times per week of participation in 16 week periods each of which was taken 40-50min. They were assessed for the Glucose, Insulin, IgA, IgG, IgM, IL-6, TNF-α, CRP in both group. The collected data were analyzed by Two-way ANOVA with repeated measure. The result indicated that. Glucose, insulin, HOMA-IR were significantly decreased in 0-8weeks(meddle intensity exercises) and 8-16weeks(high intensity exercise). Percentage of body fat, BMI showed a significant decrease in 0-8weeks(meddle intensity exercises) and 0-16weeks(middle+high intensity exercise). IgA showed a significant increase in 0-8weeks(meddle intensity exercises), 0-16weeks(middle+high intensity exercise). IgM was significantly increased in 0-16weeks(middle+high intensity exercise) and 8-16weeks(high intensity exercise). IL-6 and TNF-a were significantly decreased in 0-8weeks(meddle intensity exercises) and 8-16weeks(high intensity exercise). CRP were decreased 0-8weeks(meddle intensity exercises), 0-16weeks(meddle+high intensity exercise).

유산소 운동강도의 차이가 인슐린저항성 지표 및 인슐린 pathway에 미치는 영향

이승범 한국웰니스학회 2014 한국웰니스학회지 Vol.9 No.1

본 연구의 목적은 20주간의 유산소 운동강도의 차이가 정상집단과 비만과 인슐린저항성 집단의 Glucose, Insulin, HOMA-IR 및GLUT2., GLUT4 유전자의 발현변화에 미치는 영향을 알아보기 위해 실시하였다. 본 연구의 대상자는 40∼50세의 중년 여성으로 정상 집단(n=20)과 비만과 인슐린저항성 집단(n=20) 총 40명으로 선정하였다. 실험기간은 총 20주이고, 주 3회, 1회 운동시간은 30∼80분이며, 측정항목으로 Glucose, Insulin, HOMA-IR, GLUT2 및 GLUT4 등을 측정하였다. 자료처리방법은 반복측정 이원변량분석(two-way ANOVA with repeated measure)을 이용하였으며, 다음과 같은 결론을 도출하였다. 첫째, Glucose의 0, 10주차, 20주차를비교한 결과 집단 간에서만 유의한 차이가 나타났다(p<.022). 둘째, Insulin과 HOMA-IR의 0, 10주차, 20주차를 비교한 결과 측정 시기 간에서만 유의한 차이가 나타났다(p<.008; p<.004). 셋째, GLUT2와 GLUT4의 0, 10주차, 20주차를 비교한 결과 증가한 수치를 볼수 있었으며 시기별 10주차와 20주차에 유의한 차이가 나타났다(p<.001, p<.001). The purpose of this study is the difference in strength of 20 weeks of aerobic exercise in two??group. The subjects of thisstudy were totally 40(n=20 in the normal group, n=20 in the obesity??group). The subjects was provided with 3 times per week ofparticipation in 20 week periods each??of which was taken 30-80 minutes. They were assessed for the Glucose, Insulin, HOMA- IR,GLUT2 & GLUT4 in both group. The collected data were analyzed by Two-way ANOVA. The??result indicated that. First, Glucose,the two groups of 0, 10weeks, 20weeks on a comparison of??measurements significant difference among the groups(p<.022). Second, Insulin and HOMA-IR, the two groups of 0, 10weeks, 20weeks on a comparison of??measurements significant differencesonly between time(p<.008; p<.004). Third, GLUT2 and??GLUT4, the two groups of 0, 10weeks, 20weeks on a comparison ofmeasurements significant??differences only between time(p<.001, p<.001).

초임계유체 크로마토그래피를 이용한 지방산의 확산특성 해석

이승범,성대형,김형수,홍인권 ( Seung Bum Lee,Dae Hyung Seong,Hyung Su Kim,In Kwon Hong ) 한국공업화학회 1996 공업화학 Vol.7 No.6

지방산의 분리방법중 증류나 추출 등의 전통적인 방법을 대체할 수 있는 공정으로 초임계유체 크로마토그래피 분리법을 추천할 수 있다. 그러나 지방산을 구성하는 탄소수나 불포화도는 초임계유체내에서 확산특성을 달리 하지만 이들의 정량적인 데이터가 부족하여 초임계유체 크로마토그래피법을 공정화하는데 큰 어려움이 있다. 본 연구에서는 불포화도를 달리하는 지방산들의 초임계 이산화탄소내 확산계수를 CPB법(capillary peak-broadening method)을 기초로 온도(308.15∼328.15K)와 압력(13∼17MPa)을 변수로 하여 실험적으로 측정하였다. 이성분계 확산계수는 일정한 온도에서 압력이 증가함에 따라 감소하며 일정한 압력에서 온도가 증가함에 따라 증가하였다. 일정한 밀도에서 온도에 따른 확산계수의 영향은 크지 않으며, 밀도와 점도가 증가함에 따라 확산계수는 감소하였다. 또한 Wilke-Chang식과 Funazukuri의 실험식, Matthews-Akgerman식을 초임계 이산화탄소에서의 이성분계 확산계수에 대한 상관식으로의 이용가능성을 확인한 결과 다른 상관관계식에 비해 RHS(rough hard sphere)에 기초한 자유부피모델인 Matthews-Akgerman식이 잘 일치하는 상관식인 것으로 확인되었다. Supercritical fluid chromatographic method was recommended as an alternative separation method of fatty acids of the conventional method such as distillation or extraction. Although diffusion characteristics are varied by the carbon numbers and the degree of unsaturation of fatty acids, the quantitative data were so rare that the commercialization of supercritical fluid chromatographic method has been hindered. In this study, diffusion coefficients of fatty acids which are differently unsaturated are measured by CPB method in the range of 308.15K to 328.15K and 13MPa to 17MPa in supercritical carbon dioxide. A decrease in the binary diffusion coefficient was observed with an increase in temperature and pressure. Also, the decrease in the binary diffusion coefficient with increasing fluid density and viscosity. Wilke-Chang equation, Funazukuri empirical equation, and Matthews-Akgerman equation are used to correlate the experimental diffusion coefficients of fatty acids in supercritical carbon dioxide. Amongthe various theoretical equations, Matthews-Akgerman equation based on RHS theory was suggested as a more successful correlation model with experimental data.

초임계유체 반응매개상에서 VOCs의 촉매산화 전환특성 ( Catalytic Oxidation Conversion Characteristics of VOCs in Supercritical Fluid Media )

이승범,홍인권,이재동 대한환경위생공학회 2001 대한환경위생공학회지 Vol.16 No.4

Plastid 형질전환에 위한 의한 유용 농작물 개발

이승범,Lee, Seung-Bum 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1

Phase Behavior of Sick House Syndrome(SHS) Chemicals

이승범,홍인권,Myung Keun Shin 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3

The vapor/liquid phase equilibrium data of sick house syndrome (SHS) materials were measured experimentally. SHS materials are chemical species to which the human body is exposed, such as the volatile organic chemicals emitted from constructing finishes. In this study, acetone(1) + toluene(2) and benzene(1) + m-xylene(2), as two sets of mixed SHS, were selected in the experimental system. These SHS are used as solvents for various chemical processes and finishing materials for residential dwellings,as well as the components contained in interior construction materials. It is very important to identify their vapor phase concentration and diffusion characteristics under atmospheric pressure. The vapor phase equilibrium concentration of the mixed SHS was measured experimentally, the data were correlated with their phase behavior characteristics, and then their nonideal behaviors were predicted by means of calculating their activity coefficients. The NRTLmodel and Wilson equation were employed as the thermodynamic equilibrium prediction model, in which the parameters of the experimental system were optimized in the course of calculation. The vapor phase concentration for each pair of mixed SHS was proposed with the given temperature range.

30~60대 성인남성의 신체활동수준에 따른 대사증후군 관련 지표의 차이 - 제7기 3차(2018) 국민건강영양조사 자료를 이용하여 -

이승범,최재일 한국웰니스학회 2020 한국웰니스학회지 Vol.15 No.2

This study was conducted through the 2018 National Health and Nutrition Survey to clarify the differences in metabolic syndrome-related indicators according to the physical activity levels of adult males in their 30s to 60s. A low physical activity group (n = 1,163, 66.4%), moderate physical activity group (n = 398, 22.7%), and high physical activity group (n = 190, 10.9%) according to the physical activity level of 1,751 people As a result of analyzing the differences in indicators related to metabolic syndrome, the results were as follows. It was found that high physical activity groups performed the most strength exercises per week. And it was found that 83.2% of the low physical activity group did not perform aerobic exercise. The systolic blood pressure was found to be significantly higher in the low physical activity group compared to the high physical activity group. In addition, HbA1C, HDL-C, TC / HDL-C ratio, and triglyceride were significantly higher in low-intensity physical activity group than in moderate and high physical activity group. 이 연구는 제7기 3차(2018년도) 국민건강영양조사 자료를 통해 우리나라 30~60대 남성의 신체활동량 수준에 따른 대사증후군 관련 지표의 차이를 규명하기 위해 실시되었다. 1,751명을 대상으로 신체활동수준에 따라 저강도 신체활동 집단(n=1,163, 66.4%), 중강도 신체활동 집단(n=398, 22.7%), 고강도 신체활동 집단(n=190, 10.9%)으로 구분하여 대사증후군 관련 지표의 차이를 분석한 결과 다음과 같이 나타났다. 고강도 신체활동 집단에서 ‘1주일간 근력운동 일수’가 가장 많은 것으로 나타났고, ‘유산소 신체활동 실천율’은 저강도 신체활동 집단의 83.2%가 실시하지 않는 것으로 나타났다. 수축기 혈압의 경우 고강도 신체활동 집단에 비해 저강도 신체활동 집단에서 유의하게 높은 것으로 나타났다. 또한 당화혈색소, 고밀도 지단백 콜레스테롤, 총콜레스테롤/고밀도 지단백 콜레스테롤 비율, 중성지방 수치가 중강도, 고강도 신체활동 집단에 비해 저강도 신체활동 집단에서 유의하게 높은 것으로 나타났다.

규칙적인 유산소 운동이 쥐의 활성산소, 과산화지질 및 황산화 효소에 미치는 영향

이승범,박상규 한국사회체육학회 2004 한국사회체육학회지 Vol.0 No.22

The purpose of this study was to evaluate the effects of loaded swimming and unloaded swimming on response of reactive oxygen and antioxidant enzymes activity of rats. 30 male SD rats(6 weeks old) were randomly divided into loaded swimming group and unloaded swimming group and then swam for 8 weeks(5days/week, 50min/day). All data were expressed as mean and standard deviation by using SPSS/PC+ program, and data were analyzed by Paired t-test(α=.05), and Independent t-test test was performed to test the significant levels of differences between groups. The conclusions obtained from this study were as follows; 1. in hydroxyl radical(HR), Training group, Untraining group and Between Groups has revealed significant increases(p<.05). 2. in malondialdehyde(MDA), Training group, Untraining group and Between Groups has not revealed significant increases. 3. in superoxide dismutase(SOD) activity, Training group, Untraining group and has not revealed significant increases(p<.05). but not Between Groups 4. in glutathione peroxidase(GPX) activity, Training group, Untraining group and Between Groups has revealed significant increases(p<.05). 5. in catalase(CAT) activity, Training group, Untraining group and Between Groups has revealed significant increases(p<.05).

외부환경온도변화가 운동수행능력에 미치는 영향

이승범,전용균 한국웰니스학회 2013 한국웰니스학회지 Vol.8 No.1

이 연구는 외부환경온도변화가 운동수행능력에 미치는 영향을 알아보기 위함이다. 연구대상은 의학적으로 특별한 질환이 없는 D대학 학생 12명으로 설정하였다. 외부환경온도는 저온(5±0.5℃), 상온(20±0.5℃), 고온(33±0.5℃)에서 사전실험을 통해 산출된 O2max 60%에 해당되는 운동부하 강도로 30분간 운동을 실시하였다. 운동부하검사 중 피험자의 O2, HRmax, RER, AT의 측정은 호흡가스분석시스템(Cardio pulmonary exercise test system)을 이용하고, 젖산농도는 운동 후 회복기 5분에 핑거팁으로 채혈하여 젖산농도분석기를 이용하여 측정하였다. 자료분석은 외부환경온도변화에 따른 대사변인의 값의 평균과 표준편차를 구하고, 일원변량반복측정(on-way ANOVA with repeated measure)방법을 이용하였고, 사후검증으로는 Tukey의 다중비교방법을 사용하였으며, 모든 측정치의 유의수준은 p<.05로 설정하여 검증하였다. 이 연구의 결과는 다음과 같다. 외부환경온도변화가 최대산소섭취량, 최대심박수, 호흡교환율, 회복기 5분의 혈중젖산 농도에 미치는 영향은 통계적으로 유의한 차이가 나타났다. 사후검증 결과 고온에서 모두 높게 나타났다. 그러나 무산소성역치에서는 통계적으로 유의한 차이가 나타나지 않았다. The present study was to investigate the changes of exercise performance ability during exercise and recovery at different external environmental temperature. The subjects of the study were 12 students from D University who did not have an particular clinical disease. In the experiment, blood was collected after exercise, the subjects had warming up excercise and then 30minutes exercise at low temperature(5±0.5℃), room temperature(20±0.5℃) and high temperature(33±0.5℃) using protocol obtained from a preliminary experiment. During the exercise, respiration variables were measured at exercise performance ability such as oxygen uptake(VO2), maximum heart rate(HRmax), respiratory exchage ratio(RER) and anaerobic threshold(AT) were also measured using pulmonary exercise test system. They blood was collecte at 5minutes after exercise, and the lactic acid concentration of blood, which is a exercise performance ability was measured and analyzed. In data analysis, the means and standard deviations of exercise performance ability according to external environmental temperature were collected and analyzed by one way ANOVA with repeated measure and post-hoc using Tukey. A significant level was considered as p<.05. The results were as follows; First, among exercise performace ability during exercise, oxygen uptake(VO2), maximum heart rate(HRmax), respiratory exchange ratio(RER), 5minutes' recovery lactic acid showed a significant difference at high temperature. But anaerobic threshold(AT) did not show any significant difference according to external environmental temperature.