중증 건성안에서 헤파린과 인지질을 사용한 복합치료의 임상적 효과

주광식,전연숙,김재찬,Kwang Sic Joo,MD,Yeoun Sook Chun,MD,PhD,Jae Chan Kim,MD,PhD 대한안과학회 2010 대한안과학회지 Vol.51 No.8

Purpose: To evaluate the therapeutic effects of topical heparin (Hylo-ParinⓇ, Ursapharm Saarbr cken, Germany) and spray type phospholipids (Tears AgainⓇ, Optima Pharmazeutische GmbH. Freising, Germany) in severe dry eye syndrome resistant to conventional therapy. Methods: Twenty eyes of ten patients with refractory severe dry eye were treated with Hylo-ParinⓇ (two times a day) and Tears AgainⓇ (three times a day) for three months. Before and one and three months after treatment, a symptom questionnaire was administered to the patients. The ocular surface disease index (OSDI), tear film break-up time, Schirmer test, conjunctival fluorescein staining examinations and filamentary keratitis were evaluated. Results: After using Tears AgainⓇ and Hylo-ParinⓇ, the OSDI score improved from 64.13 ± 15.12 to 43.80 ± 15.87 (p<0.01). Tear film break-up time significantly increased from 1.0 ± 0.65 to 2.3 ± 0.73 seconds (p<0.01) and conjunctival staining score (Oxford scale) significantly decreased from 3.85 ± 0.75 to 3.25 ± 0.97 (p<0.01). Filamentary keratitis in the slit-lamp examination showed significant improvement (p<0.01). Conclusions: Tears AgainⓇ and Hylo-ParinⓇ are considered as new treatment modalities for severe dry eye syndrome and filamentary keratitis in patients with chronic ocular surface disease resistant to conventional therapy. These treatments require additional research. J Korean Ophthalmol Soc 2010;51(8):1047-1053

가람 이병기의 시조 미학

주광식 한국어문교육학회 1984 어문학교육 Vol.7 No.-

Measurement of Strabismic Angle Using the Distance Krimsky Test

주광식,구현,문남주 대한안과학회 2013 Korean Journal of Ophthalmology Vol.27 No.4

Purpose: To evaluate the correlation of the distance Krimsky test and the alternate prism cover test (APCT) for the distance deviation in patients with horizontal strabismus. Methods: Forty patients with horizontal strabismus (20 esotropia and 20 exotropia) were included in this study. Patients with a variable angle of deviation, vertical angle over 5 prism diopters, impaired binocular vision,or poor cooperation were excluded. We instructed the patient to look a target 6 meters away, and applied a prism over the patient’s dominant eye while flashing a light source 33 centimeters from the middle of both eyebrows. When the corneal light reflexes were located on the center of each cornea, we measured the angle of deviation. We defined this method as ‘distance Krimsky test,’ and the angle measured by this method was compared with the conventional Krimsky test and APCT at distance. We analyzed the accuracy and intra- and inter-observer reliability. Results: The angle of strabismus measured by the distance Krimsky test showed a significant agreement and correlation with the deviation angle measured by the APCT. We elicited the correlation gradient between the angle measured by the distance Krimsky test and the APCT. In addition, the distance Krimsky test showed significant intra- and inter-observer reliabilities. Conclusions: The distance Krimsky test is expected to be more useful than the Krimsky test in measuring the distance angle of deviation for patients with strabismus in whom it is difficult to measure the angle of deviation using the APCT. The distance Krimsky test can be an accurate and useful test through the improvement of proficiency of examiners and the establishment of individualized normative data.

기능적 눈물길 폐쇄에서 눈물길 신티그라피와 플루오레세인 염색소실검사의 유용성 비교

주광식,이정규.Kwang Sic Joo. MD. Jeong Kyu Lee. MD. PhD 대한안과학회 2011 대한안과학회지 Vol.52 No.9

Purpose: To compare the usefulness of fluorescein dye disappearance test (FDDT) and dacryoscintigraphy in functional lacrimal blockage. Methods: The present study included with 24 patients (37 eyes), who were diagnosed with functional lacrimal blockage and underwent silicone tube insertion in our clinic. Compared to postoperative symptom improvement, the results of FDDT and dacryoscintigraphy were analyzed. Results: Significant correlations were observed with FDDT and dacryoscintigraphy results in 29 eyes before surgery. In 33 eyes, there were post-operative symptom improvements and the sensitivity of each exam was estimated at 87.8% in FDDT and 90.9% in dacryoscintigraphy. After intubation normal findings were observed in each examination and the symptoms improved in 7 out of 8 eyes. Conclusions: Both FDDT and dacryoscintigraphy were considered sensitive and efficient methods in the diagnosis and evaluation of functional lacrimal blockage. Both methods require caution regarding misinterpretation by false negatives and may be complementary as well as increasing diagnostic accuracy. J Korean Ophthalmol Soc 2011;52(9):1013-1018

인천지역 유통 생약 중 Ochratoxin A 오염실태 조사

임수선,주광식,최은정,이수연,임윤선,엄선아,조남규,이성모 한국생약학회 2017 생약학회지 Vol.48 No.1

The aim of this study was to screen the contamination by ochratoxin A of mycotoxins in various medicinal herbs. We conducted a survey of ochratoxin A in medicinal herb on the retail market in Incheon in 2016. 116 medicinal herb samples were evaluated for the ochratoxin A contamination. They were analyzed for ochratoxin A using immunoaffinity column and high-performance liquid chromatography(HPLC)-fluorescence detection and the positive samples were confirmed using HPLC-tandem mass spectrometry. Ochratoxin A was detected in 4 medicinal herb samples; the concentrations of ochratoxin A were containing between 20.11 and 372.90 mg/kg. This study shows that in general, this kind of commodity may be contaminated by mycotoxins. Also this contamination is not limited to only aflatoxin of mycotoxins.

Genetic Mutation Profiles in Korean Patients with Inherited Retinal Diseases

김민석,주광식,성문우,김만진,박규형,박성섭,우세준 대한의학회 2019 Journal of Korean medical science Vol.34 No.21

Background: Because of genetically and phenotypically heterogenous features, identification of causative genes for inherited retinal diseases (IRD) is essential for diagnosis and treatment in coming gene therapy era. To date, there are no large-scale data of the genes responsible for IRD in Korea. The aim of this study was to identify the distribution of genetic defects in IRD patients in Korea. Methods: Medical records and DNA samples from 86 clinically diagnosed IRD patients were consecutively collected between July 2011 and May 2015. We applied the next-generation sequencing strategy (gene panel) for screening 204 known pathogenic genes associated with IRD. Results: Molecular diagnoses were made in 38/86 (44.2%) IRD patients: 18/44 (40.9%) retinitis pigmentosa (RP), 8/22 (36.4%) cone dystrophy, 6/7 (85.7%) Stargardt disease, 1/1 (100%) Best disease, 1/1 (100%) Bardet-Biedl syndrome, 1/1 (100%) congenital stationary night blindness, 1/1 (100%) choroideremia, and 2/8 (25%) other macular dystrophies. ABCA4 was the most common causative gene associated with IRD and was responsible for causing Stargardt disease (n = 6), RP (n = 1), and cone dystrophy (n = 1). In particular, mutations in EYS were found in 4 of 14 autosomal recessive RP (29%). All cases of Stargardt disease had a mutation in the ABCA4 gene with an autosomal recessive trait. Conclusion: This study provided the distribution of genetic mutations responsible for causing IRD in the Korean patients. This data will serve as a reference for future genetic screening and treatment for Korean IRD patients.

Pigmented Paravenous Retinochoroidal Atrophy

최원종,주광식,박규형 대한안과학회 2020 Korean Journal of Ophthalmology Vol.34 No.1

Characteristics of 681 Low Vision Patients in Korea

김재훈,주광식,문남주 대한의학회 2010 Journal of Korean medical science Vol.25 No.8

The purpose of our study was to evaluate the characteristics and the changes of low vision patients over ten years in Korea, and to establish useful data for planning low vision services, active care and rehabilitation. We conducted a retrospective study of 681 low vision patients who visited two low vision clinics in Seoul from 1995 to 2008. Age and sex distribution, cause of low vision, type of prescribed low vision aids, and changes of the characteristics were reviewed. In result, male were more than female. The age group between 11 and 20-yr-old (18.1%) was the largest age group. Optic atrophy (28.3%) was main causes of low vision. However, elderly low vision patients is increasing and macular degeneration is becoming a leading cause of low vision (P<0.05). One thousand five low vision aids (LVAs) were prescribed for 681 patients (1.46±0.62 aids for each patient). Near LVAs were prescribed more than distance LVAs. In most patients, the use of LVAs improved both near and distance visual function. This study is the first survey of a large number of low vision patients over a ten year period in Korea. On the base of this study, the planning of low vision services and more active rehabilitation for low vision patients, especially elderly patients, need in Korea.

Severe or Profound Sensorineural Hearing Loss Caused by Novel USH2A Variants in Korea: Potential Genotype-Phenotype Correlation

이상연,주광식,오자영,한진희,박혜림,이승민,오두이,우세준,최병윤 대한이비인후과학회 2020 Clinical and Experimental Otorhinolaryngology Vol.13 No.2

Objectives. We, herein, report two novel USH2A variants from two unrelated Korean families and their clinical phenotypes, with attention to severe or more than severe sensorineural hearing loss (SNHL). Methods. Two postlingually deafened subjects (SB237-461, M/46 and SB354-692, F/34) with more than severe SNHL and also with suspicion of Usher syndrome type II (USH2) were enrolled. A comprehensive audiological and ophthalmological assessments were evaluated. We conducted the whole exome sequencing and subsequent pathogenicity prediction analysis. Results. We identified the following variants of USH2A from the two probands manifesting more than severe SNHL and retinitis pigmentosa (RP): compound heterozygosity for a nonsense (c.8176C>T: p.R2723X) and a missense variant (c.1823G>A: p.C608Y) in SB237, and compound heterozygosity for two frameshift variants (c.14835delT: p.S4945fs & c.13112_13115delAAAT: p.G4371fs) in SB354. Based on the American College of Medical Genetics and Genomics/Association for Molecular Pathology guidelines, two novel variants, c.1823G>A: p.C608Y and c.14835delT: p.Ser4945fs, can be classified as “uncertain significance” and “pathogenic,” respectively. The audiogram exhibited more than severe SNHL and a down-sloping configuration, necessitating cochlear implantation. The ophthalmic examinations revealed typical features of RP. Interestingly, one proband (SB 354-692) carrying two truncating compound heterozygous variants exhibited more severe hearing loss than the other proband (SB 237-461), carrying one truncation with one missense variant. Conclusion. Our results provide insight on the expansion of audiological spectrum encompassing more than severe SNHL in Korean subjects harboring USH2A variants, suggesting that USH2A should also be included in the candidate gene of cochlear implantation. A specific combination of USH2A variants causing truncating proteins in both alleles could demonstrate more severe audiological phenotype than that of USH2A variants carrying one truncating mutation and one missense mutation, suggesting a possible genotype-phenotype correlation. The understanding of audiological complexity associated with USH2A will be helpful for genetic counseling and treatment starategy.

Retinitis Pigmentosa Associated with Bardet-Biedl Syndrome with BBS9 Gene Mutation in a Korean Patient

김용훈,주광식,성문우,박성섭,우세준 대한안과학회 2020 Korean Journal of Ophthalmology Vol.34 No.1