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유준희,정구흥 韓國生物敎育學會 1994 생물교육 Vol.22 No.2
Recombinant DNA technology was applied to scientifically gifted high school students using developed laboratory teaching materials. As a result, they successfully performed all experiments. Through laboratory activities they showed a significant increase in understanding of concepts of genetic engineering (p=0.007). In the survey of questionnaire it was known that they were strongly influenced on intellectual inquiry and interest of biology through laboratory activities of recombinant DNA technology. In questionnaire ninety four percents of students answered that laboratory activities of recombinant DNA technology helped their understanding about genetic engineering, and students hoped that experiments of recombinant DNA technology were included into the content of textbook.
이선민,정구흥 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.4
Since DNA contains the genetic information of an organism, accurate DNA replication is one of the most important events of the life cycle of an organism. DNA polymerases are key enzymes catalyzing the accurate replication of DNA. DNA polymerases have been classified, based on their amino acid sequence similarities, into two major groups: family A and family B. The family B DNA polymerases share regions of highly conserved amino acid sequcences. These conserved regions occur in the Same order in all family B DNA polymerases molecules. Therefore, it is likely that these sequence conservations are a consequence of their contribution to the DNA polymerase function and structure. However, little is known at present about functional roles of these highly conserved regions. To determine the functional roles of the highly conserved regions of the family B DNA polymerases, amino acid change generated in conserved regions by the site-directed mutagenesis. The results show that mutations at the conserved regions within PRDI DNA polymerase inactvate polymerasc complementing activity and catalytic activity. A new conserved region between DNA polymerase family A and B in the N-terminal portion has been identified which contains three highly conserved amino acids known to be involved in the 3'-5'exonuclease active site of Klenow fragment of E. aoli polymerase I.
유준희,정구흥 韓國生物敎育學會 1993 생물교육 Vol.21 No.2
Inspite of students' curosity and social need for genetic engineering there are not actual experiments related with it in biology textbook of high school. A basic laboratory manual of recombinant DNA technology was developed to understand concepts of genetic engineering. The laboratory manual consisted of contents including basic microbial technology to manupulation of DNA. Using developed laboratory manual, it was applied to biology teacher of secondary school, Through activities of many experiments, they showed significant increase in understanding of concepts(p=0.000). Teachers agreeded with the need of experiments of recombinant DNA technology in science high school, However, comparing to scientifically gifted high school students biology, teachers were lower in achievement and interests about experimental activity. It suggests that teacher group need enough explanations and experimental time to understand concepts of recombinant DNA technology.
Sugar Constituents of Jalapin from Sweet Potato Tubers
이서래,정구흥,김호식,SU RAE LEE,KOO HEUNG CHUNG,HO SIK KIM Korean Chemical Society 1969 대한화학회지 Vol.13 No.1
고구마중 잘라핀의 糖構成을 究明하기 위하여 고구마塊根에서 정제한 잘라핀을 脫아실化한 후 가수분해한 결과 L-rhamnose, D-fucose, D-glucose가 1:1:1의 分子比 및 上記順序의 酸安定度를 가짐을 알았다. 脫아실化한 잘라핀 1몰은 2몰을 過沃素酸에 의하여 산화되었으나 D-glucose는 산화되지 아니하였다. 따라서 고구마중 脫아실化한 잘라핀의 가능한 化學構造를 다음과 같이 제안하였다. L-$Rha_f$-(1${\to}$4)-D-$Fuc_p$-(1${\to}$3)-D-$Glu_p$-(1${\to}$11)-jalapinolic acid. Jalapin purified from the tubers of the sweet potato (Ipomoea batatas) was deacylated and subjected to structural elucidation. Complete and degraded acid hydrolyses indicated the presence of L-rhamnose, D-fucose and D-glucose in the molar ratio of 1: 1: 1 and in the increasing order of acid-stability. While two moles of periodate were consumed per mole of the product, D-glucose survived in the oxidation. The following structure was, therefore, proposed tentatively for the deacylated jalapin: L-$Rha_f$-(1${\to}$4)-D-$Fuc_p$-(1${\to}$3)-D-$Glu_p$-(1${\to}$11)-jalapinolic acid.
Aspergillus nidulans 형질전환체의 Genomic DNA 분석
김석준,유준희,정구흥 한국유전학회 1993 Genes & Genomics Vol.15 No.4
Vectors with Clostridium thermosulfurogenes α-amylase gene as a foreign gene and E. coli hph gene as a selectable marker gene which represented hygromycin B resistance(HmB^R) were constructed. AlcA promoter and trpC terminator of Aspergillus nidulans were used on these vectors. Sensitivity of Aspergillus nidulans KCTC6034 to HmB was checked. A. nidulans did not grow at the plates with 600㎍/㎖ of HmB. A. nidulans was transformed with these vectors. Twenty two transformants were obtained and screened by polymerase chanin reaction with genomic DNA to confirm the existence of the foreign DNA. Most of transformants had hph gene sequence and at least 26% of transformants had α-amylase gene sequence.