Solvent Effects on Cis - trans Photoisomerization of The Indigoids and The Polyenes

이태녕 ( Tae Young Lee ) 한국공업화학회 1990 한국공업화학회 연구논문 초록집 Vol.1990 No.3

L - Ascorbic Acid 가 Guinea Pig 에서 혈장 지질과산화값 , Prostaglandin 생합성 , 광용혈 , Superoxide Dismutase 및 Catalase 활성에 미치는 영향

이정원,이태녕,모수미,이진호,이대형,박수남,이보경 ( Joung Won Lee,Tae Young Lee,Sumi Mo,Jin Ho Lee,Dae Hyung Lee,Soo Nam Park,Bo Kyung Lee ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.4

Three levels of L-ascorbic acid(AsA) were orally given to 20 male Hartley guinea pigs weighing average 428 g for five weeks. AsA level was 0, 2.5, and 100 mg per 100 g body weight per day in the deficient, the adequate, and the excess group, respectively. Plasma lipid peroxide value(TBA value) of deficient was increased to the highest among the three groups and both the adequate and the excess groups exhibited the lower TBA values, suggesting the antioxidant action of AsA. In the excess of AsA, plasma TBA value was greatly lowered one day after the feeding, but TBA value after 5 weeks was elevated comparable to that of the adequate. Aspirin administrating experiment suggested that this elevation was due to increased. production of prostaglandin(PG) endoperoxides induced by excess AsA. Thus it could be concluded that excess AsA actually inhibited the tissue lipid peroxidation in vivo. The activities of both superoxide dismutase(SOD) and catalase in erythrocytes demonstrated the elevations in the deficient comparing with the auequate, but not any significant variations in the excess. SOD activities were correlated with TBA value in liver (r = .324) and with percent hemolysis in erythrocytes (r = .323). Photohemolysis of erythrocyte was increased not only in the AsA deficient but also in the excess comparing with the adequate. A new finding that the large excess administration of AsA in guinea pig demonstrated a significant stimulation of PG endoperoxide biosynthesis, would suggest an important biochemical implication on vitamin C as an activator of phospholipase A₂ and/or PG endoperoxide synthetase. The stimulation of PG biosynthesis might be associated with the enhanced photohemolysis, and this problem needs to be further investigated.

L-Ascorbic Acid가 Guinea Pig에서 혈장 지질과산화값, Prostaglandin 생합성, 광용혈, Superoxide Dismutase 및 Catalase활성에 미치는 영향

이정원,이태녕,모수미,이진호,이대형,박수남,이보경,Lee, Joung-Won,Lee, Tae-Young,Mo, Su-Mi,Lee, Jin-Ho,Lee, Dae-Hyung,Park, Soo-Nam,Lee, Bo-Kyung 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.4

L-Ascorbic acid(AsA)를 결핍, 석정, 과잉(적정의 40배)의 세 수준으로 나누어 guinea pig(평균체중 428g, male)에게 5주동안 경구 투여했을때, 적정 및 과잉으로 공급된 AsA는 AsA 결핍군에서 나타난 혈장 및 간조직의 매우 상승된 지질과산화값(TBA값)을 저하시켰으며 이 효과는 과잉공급시 더욱 컸다. 이는 AsA가 항산화작용을 통해 지질 과산화를 억제하였음을 의미한다. 그러나 과량군의 혈장 TBA값은 적정군의 혈장 TBA값 수준에 머물렀는데 이는 과잉의 AsA가 동시에 혈장 TBA값에 포함되어지는 prostaglandin (PG) endoperoxide의 생합성을 촉진했기 때문이다. 그리고 superoxide dismutase(SOD)와 catalase 활성은 AsA결핍시 특히 적혈구에서 증가경향을 보였으나 과잉 공급인 경우엔 별 변화가 없었다. 동물 개개의 SOD활성도는 간조직에서는 TBA값과 (r = .324) 적혈구에서는 용혈수준과 (r = .323) 상관관계 (p < 0.1)가 있었다. AsA의 대량공급이 지질의 과산화반응을 억제한다는 의의 이외에 PG생합성의 촉진수단으로서 중요한 구실을 할 수 있다는 주목할만한 vitamin C의 새로운 생화학적 의의를 시사한다. 또한 AsA의 과량공급은 적혈구의 $^1O_2$에 의한 광용혈을 적정량 공급군에 비해 촉진시키는 결과를 보이고 있다. 이 광용혈 촉진의 생화학적 의의는 PG endoperoxide 생합성 활성화의 결과와 연관성이 있을 것으로 보이며 PG endoperoxides 촉진문제와 더불어 앞으로 검토해야 될 문제로 사료된다. Three levels of L-ascorbic acid(AsA) were orally given to 20 male Hartley guinea pigs weighing average 428 g for five weeks. AsA level was 0, 2.5, and 100 mg per 100g body weight per day in the deficient, the adequate, and the excess group, respectively. Plasma lipid peroxide value(TBA value) of deficient was increased to the highest among the three groups and both the adequate and the excess groups exhibited the lower TBA values, suggesting the antioxidant action of AsA. In the excess of AsA, plasma TBA value was greatly lowered one day after the feeding, but TBA value after 5 weeks was elevated comparable to that of the adequate. Aspirin administrating experiment suggested that this elevation was due to increased. production of prostaglandin(PG) endoperoxides induced by excess AsA. Thus it could be concluded that excess AsA actually inhibited the tissue lipid peroxidation in vivo. The activities of both superoxide dismutase(SOD) and catalase in erythrocytes demonstrated the elevations in the deficient comparing with the auequate, but not any significant variations in the excess. SOD activities were correlated with TBA value in liver (r = .324) and with percent hemolysis in erythrocytes (r = .323). Photohemolysis of erythrocyte was increased not only in the AsA deficient but also in the excess comparing with the adequate. A new finding that the large excess administration of AsA in guinea pig demonstrated a significant stimulation of PG endoperoxide biosynthesis, would suggest an important biochemical implication on vitamin C as an activator of phospholipase $A_2$ and/or PG endoperoxide synthetase. The stimulation of PG biosynthesis might be associated with the enhanced photohemolysis, and this problem needs to be further investigated.

무산소상태에서 FeCl₃로 촉진된 Thiobarbituric Acid 반응을 이용한 혈청중 TBA반응물의 새로운 정량법

이정원(Lee Joungwon),모수미(Mo Sumi),이태녕(Lee Tae Young) 韓國營養學會 1984 Journal of Nutrition and Health Vol.17 No.1

Sucrose를 첨가한 김치의 발효시 생성되는 점성물질의 본성에 대하여

한영숙(Young-Sook Hahn),우경자(Kyung-Ja Woo),박영희(Young-Hee Park),이태녕(Tae-Young Lee) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.2

김치 담글 때 조미료로 sucrose를 첨가한 경우, 발효 시간의 경과와 더불어 김치국물의 정도가 증가한다. 이 현상은 장김치에서 두드러지는 것으로 관찰되었으므로 본 연구에서는 소금으로 담근 김치를 대조구로 하여 간장으로 담근 장김치에서의 정도 증가를 비교하였으며 이 점성물질의 본성을 밝힘과 동시에 그 생성조건을 검토하였다. 장김치국물에 에탄올을 가하여 생성된 침전물을 산으로 가수분해하여 HPLC로 분석한 결과 glucose를 단위체로 한 polysaccharide인 dextran으로 밝혀졌다. 이 dextran의 생성에는 glucose 보다는 sucrose가 효과적이며, sucrose의 농도가 10%까지 증가함에 따라 점도는 비례하여 증가하였다. 또 소금보다는 간장으로 담근 김치에서 정도가 높았으며, 협기적인 조건보다는 호기적인 조건에서 점도가 높았다. 장김치는 소금김치 보다 산생성이 많았으며 당을 첨가한 경우 산 생성량은 증가하였다. 또 환원당량은 sucrose를 첨가한 경우 김치숙성 초기에 크게 증가하였다. 김치발효과정 중 총 균수와 Leuconostoc 세균수의 변화를 살펴 본 결과 발효 1일째에 Leuconostoc 균수, 총 균수가 최대에 달했으며 Leuconostoc 균수는 그 후 점차 감소하였다. 장김치와 소금김치 간에는 발효 중 균수의 차이가 없었으며 sucrose를 첨가한 경우에도 큰 차이는 없는 것으로 나타나 김치에서 점성물질이 생성되는 것은 균수에 의한 차이가 아니라 점성물질 생성 조건의 차이에 의한 것으로 생각되었다. Jangkimchi is a kind of Kimchi which is made with soy-sauce instead of salt. Occasionally, when sugar is added to Jangkimchi for condiment, the kimchi juice becomes viscous. In this study. the nature of the viscous material and the condition for producing viscous property in Kimchi juice were investigated. HPLC analysis showed that the viscous material in Jangkimchi is polysaccharide composed of glucose. Sucrose was more effective in forming viscous juice than glucose and the viscosity increased with the addition of sucrose up to 10%. Soy-sauce also played a role in increasing the viscosity of Kimchi juice compared with salt. Aerobic fermentation condition was found to be another factor to make the juice viscous. The population of Leuconostoc mesenteroides, well-known producer of viscous dextran was not different in the Kimchi juice prepared with the addition of sucrose from that without sucrose, which implys that the Jangkimchi preparation methods such as addition of sucrose and soy-sauce would do some effects on the production of viscous material in Jangkimchi.

혈청 지질단백질중의 2-Thiobarbituric Acid 반응물질에 미치는 $Fe^{3+}$의 영향

이정원,모수미,이태녕,Lee, Joung-Won,Mo, Su-Mi,Lee, Tae-Young 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.4

2-Thiobarbituric acid (TBA) test, as indirect method, has recently been used for the determination of lipid peroxidation in biological systems including blood. Together with the low sensitivity, lacks of the specificity and the understanding the reaction mechanism, however, have limited the usefullness of TBA test. Under the nitrogen atmosphere $FeCl_3$ showed great enhancement effect on the sensitivity of TBA reaction with serum protein precipitate involving lipid peroxides, but not with tetraethoxypropane. Thus ferric ion was thought to catalize the releasing TBA reactive chromogenic substance, malondialdehyde, from its precursors. The amount of ferric ion required seemed to be proportional to that of serum tested. Also, oxygen should be eliminated in serum TBA reaction so that the reaction could reach at stabe maximum and keep its plateau value. Under anaerobic atmosphere, albumin and amino acids (methionine and glutamic acid) may not contribute significantly to the great enhancement of serum TBA value induced by ferricion.

혈청 지질단백질중의 2 - Thiobarbituric Acid 반응물질에 미치는 Fe3+ 의 영향

이정원,모수미,이태녕 ( Joun Gwon Lee,Su Mi Mo,Tae Young Lee ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.4

2-Thiobarbituric acid (TBA) test, as indirect method, has recently been used for the determination of lipid peroxidation in biological systems including blood. Together with the low sensitivity, lacks of the specificity and the understanding the reaction mechanism, however, have limited the usefullness of TBA test. Under the nitrogen atmosphere FeCl₃ showed great enhancement effect on the sensitivity of TBA reaction with serum protein precipitate involving lipid peroxides, but not with tetraethoxypropane. Thus ferric ion was thought to catalize the releasing TBA reactive chromogenic substance, malondialdehyde, from its precursors. The amount of ferric ion required seemed to be proportional to that of serum tested. Also, oxygen should be eliminated in serum TBA reaction so that the reaction could reach at stabe maximum and keep its plateau value. Under anaerobic atmosphere, albumin and amino acids (methionine and glutamic acid) may not contribute significantly to the great enhancement of serum TBA value induced by ferric ion.

연어알에서 분리한 Carotenoprotein의 구조적 특성

김재웅,민태진,이태녕,Jae-Woong Kim,Tae-Jin Min,Tae-Young Lee 대한화학회 1988 대한화학회지 Vol.32 No.4

Carotenoprotein from Salmo Salar eggs was purified and characterized by CM-cellulose, 50% $(NH_4)_2SO_4$, DEAE-cellulose and sephadex G-75 column. The chromoprotein had a spectrum with ${\lambda}_{max}$ 409, 540 and 580nm in p-buffer (pH 7.0) at initial step. Molecular weights by sephadex G-200 gel filtration were 50, 200 and 26,000 daltons. SDS-PAGE analysis showed a structure with four identical subunits (12,500 daltons). Its sample retained a small amount of carbohydrates and lipids. Amino acids were analyzed, and mannose, galactose and glucosamine also were identified. Carotenoid extacted with acetone was found to be astaxanthin ester by partition test, epoxy test, iodine test, allylic test, reduction, acetylation, uv/vis, ir and nmr datas. Stearate (47.9%) and palmitate (21.4%) were predominant fatty acids in the astaxanthin ester. 연어알(Salmo Salar)에서 carotenoprotein을 CM-세룰로스, 50% 황산암모늄 침전, DEAE-세룰로스 및 세파덱스 G-75젤을 사용하여 충분히 정제하였다. 인산염 완충용액(pH 7.0)에서 자외선 흡수극대는 ${\lambda}_{max}$ 409, 540 그리고 580nm이었다. 세파덱스 G-200젤 여과법으로 측정된 분자량은 50, 200rhk 26,000달톤으로 heterogeneous한 두가지 형태였다. 전자는 적자색으로 SDS-PAG 전기영동 결과 분자량이 12,500달톤인 4개의 동일한 subunit로 구성되었고, 상당량의 지질과 만노오스, 갈락토스와 글루코사민을 함유하고 있음을 밝혔다. carotenoprotein의 색소를 추출하여 tlc로 정제한 다음 분배비 시험, 요오드 시험, 에폭시 그룹 시험, 알릴릭 시험, 환원 시험 그리고 아세틸화 시험등의 유기반응과 uv/vis, ir 및 nmr 데이타를 얻어 구조를 결정한 결과 carotenoid는 astaxanthin ester임을 확인하였으며 그 ester는 스테아르산(47.9%)과 팔미트산 (21.4%)이 대부분이었다.

담금수의 수질이 열무 물김치의 비타민 C 함량에 미치는 영향

김유진(Yoo-Jin Kim),오지영(Ji-Young Oh),이태녕(Tae-Young Lee),한영숙(Young-Sook Hahn) 한국식품과학회 1998 한국식품과학회지 Vol.30 No.1

광용혈에 대한 플라보노이드의 세포막 보호작용에 관한 연구

박수남 ( Park Soo-nam ),부용출 ( Boo Yong-chool ),이태녕 ( Lee Tae-young ) 대한화장품학회 1989 대한화장품학회지 Vol.15 No.1

Protective effects of flavonoids on cell membrane in the photohemolysis experiments. The protective effects of 24 flavonoids against singlet oxygen - induced photohemolysis of rabbit erythrocytes were investigated and compared with their antioxidant activities. 1. All the flavonoid aglycons investigted besides flavone and 7, 8- benzoflavone showed thd dose - dependant protective effects. Especially galagin showed the most pronounced effect under the experimental condition. 2. The order of the radical- scavenging activities of flavonoids was as follow (-)EGCG) flavonols)flavanols)flavones)flavanonols. The radical- scavenging activities of flavonoid glycosides were not different from those of their aglycons. 3. When added after irradiation, flavonoids did not alter the photohemolysis pattern of control. In this study, several antioxidant were found and it was newly found that photohemolysis experiment is a convenient, reproductive diagnostic method in the screening and researching the compound which has antioxidant activity or protective effect on cell membrane against active oxygen species.