자동무수차점 방식 널 렌즈 광학계를 이용한 직경 450 mm(f/2.7) 포물면경의 제작 및 측정 평가

이영훈,조재흥,임천석,이윤우,양호순,이재협,이인원,Lee, Young-Hun,Jo, Jae-Heung,Rim, Cheon-Seog,Lee, Yun-Woo,Yang, Ho-Soon,Lee, Jae-Hyeob,Lee, In-Won 한국광학회 2006 한국광학회지 Vol.17 No.2

직경 450 mm(f/2.7) 포물면경 제작을 위해 자동무수차점방식의 널 광학계를 설계 및 제작하였으며, 설계프로그램(CODE V)의 공차분석기법을 이용하여 널 광학계의 제작과 정렬 오차에 따른 측정 신뢰도를 이론적으로 검증하였다. 그리고 광학계를 실제로 구축하여 포물면경의 제작에 적용하였다. 또한, 널 렌즈를 사용하지 않고 평면거울만 사용하는 자동시준방식의 측정방법으로 포물경을 재평가하여, 역으로 자동무수차점방식의 널 렌즈 정렬오차에 의한 측정 신뢰도를 확인하였다. The autotstigmatic null lens system is designed and constructed for the fabrication of a parabolic mirror with the diameter of 450 mm(f/2.7). And the measurement reliability is also analyzed theoretically by means of the tolerancing technique using lens design software(CODE V). From this analysis, we can precisely fabricate a parabolic mirror with the large diameter of 450 mm(f/2.7). Meanwhile, in order to confirm the fabrication results by the autostigmatic method, the mirror surface is tested again by an autocollimating method that uses only a plane mirror without any null lens.

포도와 오이즙액을 이용한 동충하초(Paecilomyces japonica) 균사체 배양액의 미백효과

이영훈,박상상,이상원,이성호,박기훈,최영주,갈상완,Lee, Young-Hoon,Park, Sang-Sang,Lee, Sang-Won,Lee, Sung-Ho,Park, Ki-Hoon,Choi, Young-Ju,Gal, Sang-Wan 한국생명과학회 2006 생명과학회지 Vol.16 No.5

포도와 오이즙액에 동충하초 균사체 배양액의 미백화장품 원료로서의 이용가능성을 조사하였다. B16BL6 mouse melanoma cell에 동충하초 균사체 배양액을 배양기간 및 농도별로 처리했을 때, 15일 동안의 배양조건과 $50\;{\mu}l/ml$ (5%)의 농도로 처리하였을 때 멜라닌 생성억제 효과가 가장 높은 것을 확인하였다. In vitro tyrosinase 활성억제효과에서는 포도와 오이즙액에 동충하초 균사체 배양액을 10% 농도로 첨가시 50%의 멜라닌 생성 억제율을 보였으며, 30% 농도에서는 100% 억제를 확인하였다. 결과적으로 포도와 오이즙액에 동충하초 균사체 배양액은 멜라닌 생성의 주 효소인 tyrosinase의 작용을 억제함으로써 미백 효과를 나타내는 것을 확인하였다. 또한 세포독성실험에서 포도, 오이즙액과 그 즙액에 동충하초 균사체 배양액을 각각의 농도로 B16BL6 mouse melanoma cell에 처리 한 결과 포도, 오이즙액의 5% 처리 시에는 50% 사멸, 9% 이상에서는 100% 세포가 사멸하는 것을 확인 할 수 있었다. 그러나 포도, 오이즙액에 동충하초 균사체를 배양한 배양액은 20% 까지 처리 시에도 세포독성이 나타나지 않는 것을 확인 하였다. 따라서 포도와 오이즙액에 동충하초 균사체를 배양한 배양액은 세포 독성이 없으며, 미백 효과가 뛰어난 것으로 미백화장품 원료로서 사용가능함을 확인하였다. This study was carried out to investigate the whitening effect of mycelial culture broth of P. japonica in the mixture of cucumber and grape extracts. In the inhibition test of melanin biosynthesis of melanoma cell, B16BL6, the culture broth of P. japonica more then $50\;{\mu}l/ml$ (5%) concentration inhibited the melanin biosynthesis of the cell entirely without cytotoxicity. More then 10 days incubation of P. japonica in the mixture was required to have the inhibition activity. In vitro inhibition test of melanin biosynthesis of the culture broth of P. japonica was investigated in the concentration dependent manner of 10% to 50%. 30% concentration of the culture broth inhibited completely tyrosinase activity. In the cytotoxicity test, cucumber and grape extract itself has a strong cytotoxicity to the melanoma cell, B16Bl6. The value of $IC_{50}$ of the cucumber and grape extracts against the melanoma cells was 5% concentration. However, the culture broth of P. japonica incubated in the cucumber and grape extracts did not show the cytotoxcity up to 20% against melanoma cell, B16BL6. Therefore, we concluded that the culture broth of P. japonica in the mixture of cucumber and grape extracts can be used as a whitening cosmetic resource.

다양한 수술방법에 의한 진성악관절강직증의 치험례

이영훈,이상철,김여갑,류동목,이백수,윤옥병,Lee, Young-Hoon,Lee, Sang-Chull,Kim, Yeo-Gab,Ryu, Dong-Mok,Lee, Baek-Soo,Yoon, Ok-Byung 대한악안면성형재건외과학회 1999 Maxillofacial Plastic Reconstructive Surgery Vol.21 No.3

TMJ ankylosis is defined as a mobile disorder of jaw such as mouth opening limitation, limitation of anterior or/and lateral movement of TMJ. Kazanjian published first clinical report about classification of TMJ ankylosis dividing with intracapsular ankylosis and extracapsular ankylosis. TMJ ankylosis is resulted from trauma, infection, metastatic tumor, irradiation, burn and etc. When TMJ ankylosis is manifested in growing period, it affects to functional disorder and development and position of mandible, so it can result in maxillofacial deformity such as facial asymmetry, micrognathia, malocclusion. For treatment of TMJ ankylosis, various surgical interventions were devised ; condylectomy, gap arthroplasty, interpositional arthroplasty and TMJ reconstruction. So, we report our results with documental study and cases of true ankylosis in our department.

3T3-L1 지방전구세포에 대한 다양한 주파수 진동의 지방 생성 억제 효과

이영훈,이석호,정혜빈,정용찬,김민환,이은미,김지현,Lee, Yeong Hun,Lee, Seok-Ho,Jung, Haebin,Jung, Yong Chan,Kim, Min Hwan,Lee, Eun Mi,Kim, Chi Hyun 대한의용생체공학회 2021 의공학회지 Vol.42 No.1

Vibration is a mechanical cue that can be applied to adipose tissues for the purpose of treating obesity. However, the exact correlation between vibration and other anti-adipogenic pathways, such as development of cytoskeleton and apoptosis, remains unknown. The objective of this study was to investigate the unknown anti-adipogenic effects of vibration with varied frequencies on preadipocytes. 3T3-L1 preadipocytes were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 5% calf serum at 37 ℃ with 5% CO2 in a humidified incubator. Vibration was generated using Arduino Uno microcontroller and vibration motor module with 1 V DC, and applied to preadipocytes for 3 days. Frequency conditions were set to 20, 55, and 90 Hz. Then, the expressions of p38 pathway, ROCK-1, α-actinin, Bax, Bcl-2, caspase-9, 8, and 3 were analyzed with western blot. As a result, p38 pathway was inhibited in 55 and 90 Hz while ROCK-1 and α-actinin were expressed in 20 Hz. Caspase-3, a terminal apoptotic factor, was activated in 20 Hz via extrinsic pathway rather than intrinsic pathway. Results suggest that various frequencies of vibration can inhibit adipogenesis via different pathways which sheds light on future mechanotransduction applications of vibration for the treatment of obesity.

Recognition of Internal Restriction Sites in DNAs Cloned into M13 Vectors

이영훈,프랜시스 제이 쉬미트,Lee, Young-Hoon,Francis, J. Schmidt 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.3

DNA fragments cloned in opposite orientations into M13 vectors were allowed to associate, generating partially hybridized structures containing both double-stranded insert DNA and single-stranded vector DNA. When these structure ("Figure 8 DNA") were treated with restriction enzymes, HaeIII, HinfI, or Sau3AI, the insert DNA was cleaved preferentially to yield double-stranded fragments and single-stranded vector DNA. The action of Sau3AI was examined in detail; the results indicate that neither relaxation nor nonspecific cleavage of the "Figure" DNA was rate-limiting in the reaction. The BamHI site in the associated polylinker sequence of "Figure 8" DNA was cleaved by Sau3AI but not BamHI, suggesting that DNA structure mediated enzyme action at this site. Double-stranded fragments derived by this procedure from the insert region were used to prime dideoxy sequencing reactions in the clones from which they were derived. A sequencing scheme for long DNAs cloned into single-stranded vectors is proposed. M13 벡타에 반대방향으로 클로닝된 DNA 절편이 서로 결합한 이중가닥 DNA와 단일가닥 벡타 DNA를 포함하고 있는 부분적으로 혼성체화 된 구조를 형성하게 하였다. 이 혼성체 구조를 제한효소 HaeIII, HinfI 또는 Sau3AI으로 처리하였을 때 삽입된 DNA가 선택적으로 절단되어 이중가닥 DNA 절편과 단일가닥 벡타 DNA가 생성되었다. 이 중 Sau3AI의 작용을 자세히 조사하였다. 그 결과 이 반응에서 8자형 DNA의 이완작용이나 비선택적 절단이 속도결정단계가 아니라는 것을 밝혔다. 8자형 DNA에서 결합된 다중연결자에 존재하는 BamHI 자리는 Sau3AI에 의하여 절단되지만 BamHI에 의해서는 절단되지 않는다. 이 사실은 이 자리에서의 DNA 구조가 효소작용에 영향을 미친다는 것을 보여준다. 삽입된 DNA로부터 유도된 이중가닥 DNA 절편을 이 방법으로 얻어 디데옥시 염기결합 순서 결정법의 시발물질로 사용하였다. 이를 이용하여 단일가닥 벡타에 클로닝된 긴 DNA의 염기결합 순서를 결정하는 방법을 제안하였다.

구강종양 골절제 환자의 임플란트 수복 시 연조직에 대한 고려사항

이영훈,고경호,허윤혁,박찬진,조리라,Lee, Young-Hoon,Ko, Kyung-Ho,Huh, Yoon-Hyuk,Park, Chan-Jin,Cho, Lee-Ra 대한치과보철학회 2017 대한치과보철학회지 Vol.55 No.4

구강종양을 절제한 경우 악안면 구조의 상실로 인해 발음, 저작, 심미 등 기능장애를 유발하게 되므로 기능회복을 위해 보철 수복이 필요하다. 이때 임플란트를 이용하면 보철물의 효과적인 기능 향상을 기대할 수 있다. 하지만 골절제술을 시행한 후, 이식골의 성공률과는 별개로 연조직이 감염에 취약한 형태를 갖기 쉬우며 이식골은 염증에 이환된 경우 빠른 속도로 파괴가 발생할 수 있다. 성공적인 보철치료를 위해서는 보철치료에 앞서 적절한 연조직 처치와 보철수복 후 잦은 정기검진을 통해 임플란트의 예후와 연관된 각별한 주의가 필요하다. After the resection of oral tumor, defected maxillofacial structure caused functional difficulties including phonetics, mastication and esthetic aspects. In this cases, implant retained prosthesis can contribute to the functional enhancement. Regardless of the success rate in grafted bone, however, the soft tissue usually had a shape which was susceptible to inflammation. Moreover, infected graft bone presented rapid destruction. For success of the prosthetic treatment, adequate soft tissue treatment and frequent recall check are the essential factors to the successful implant prognosis.

자동차 부품 리사이클링 현황과 전망

이영훈,김명한 ( Y . H . Lee,M . H . Kim ) 한국주조공학회 1993 한국주조공학회지 Vol.13 No.1

고효율 광대역 마이크로스트립 대역통과필터 특성

이영훈,Lee, Young-Hun 한국정보통신학회 2009 한국정보통신학회논문지 Vol.13 No.4

본 논문에서는 구형공진기와 SIOS를 사용하여 삽입손실이 작고, 차단특성이 아주 좁고, 광대역 특성을 갖는 마이크로스트립 대역통과필터를 연구하였다. 계단 입피던스 스터브는 일반$0.25{\lambda}$ 스터브 보다 약 30%의 길이를 줄일 수 있고, 또한 스터브의 임피던스의 크기를 변경할 수 있는 장점이 있음으로 필터의 특성 개선에 적용할 수 있다. 본 논문의 객관성을 입증하기 위해서 이중모드 구형공진기를 사용한 최적화된 광대역 대역통과 필터를 구현하였다. 전송선로 모델을 사용하여 계산한 주파수 특성 결과는 실험 값과 아주 잘 일치하였다. 구현된 필터의 주파수 특성은 $L_4$ = 1.94mm인 경우에 필터의 3dB 대역폭은 52.5%(3.267GHz)이고, 삽입 손실은 0.33dB, 30dB 차단특성은 저주파수 대역에서 221MHz, 고주파수대역에서 0.181MHz이다. 최대 차단특성은 저주파 대역에서 -54dB, 고주파 대역에서 -60dB이다. This paper presents a compact, low insertion loss, sharp rejection and wide band microstrip band pass filter that is composed rectangular loop resonator and step-impedance-open-stub(SIOS) for wireless data communication. The SIOS can be reduce length about 30% more than general $0.25{\lambda}$ stub. And the stub can the advantage of tuning impedance magnitude. In order to demonstrate agrement of this paper prove, the optimized wide band pass filters are realized and experimented. A transmission line model used to calculate the frequency response of the new filters shows good agreement with measurements. The filter has 3dB fractional bandwith of 52.5%(3.267GHz), an insertion loss of better than 0.33dB from 4.587GHz to 7.854GHz, and two rejection of greater than 30dB within 221MHz$(4.366GHz{\sim}4.587GHz)$ at low frequency band, 181MHz$(7.854GHz{\sim}8.035GHz)$ at high frequency band. Maximum rejection characteristics of the filter are -54dB at low frequency and -60dB at high frequency.

그래픽 모사기법을 이용한 하천 변천의 재현과 예측

이영훈,Lee, Young-Hoon 대한자원환경지질학회 1994 자원환경지질 Vol.27 No.6

A case study is presented where a fluvial system is modeled in three dimensions and compared to data gathered from a study of the Arkansas River. The data is unique in that it documents changes that affected a straight channel that was excavated within the river by the U.S. Army Corps of Engineers. Excavation plan maps and sequential aerial photographs show that the channel underwent massive deposition and channel migration as it returned to a more natural, meandering path. These records illustrate that stability of fluvial system can be disrupted either by catastrophic events such as floods or by subtle events such as the altering of a stream's equilibrium base level or sediment load. SEDSIM, Stanford's Sedimentary Basin Simulation Model, is modified and used to model the Arkansas River and the geologic processes that changed in response to changing hydraulic and geologic parameters resulting from the excavation of the channel. Geologic parameters such as fluid and sediment discharge, velocity, transport capacity, and sediment load are input into the model. These parameters regulate the frequency distribution and sizes of sediment grains that are eroded, transported and deposited. The experiments compare favorably with field data, recreating similar patterns of fluid flow and sedimentation. Therefore, simulations provide insight for understanding and spatial distribution of sediment bodies in fluvial deposits and the internal sedimentary structure of fluvial reservoirs. These techniques of graphic simulation can be contributed to support the development of the new design criteria compatible with natural stream processes, espacially drainage problem to minimize environmental disruption.

초고속 자기부상철도 개발을 위한 시스템엔지니어링 계획서

이영훈,민성기,Lee, Young-Hoon,Min, Sung-Ki 한국시스템엔지니어링학회 2009 시스템엔지니어링학술지 Vol.5 No.1

The systems engineering (SE) process shall be applied to the project for successful development of ultra-high-speed (UHS) Maglev train system which is one of very large and complex systems. It is important to abolish technology differentials from the advanced developers such as Germany, Japan, etc. and to ensure discriminatory competitiveness of the application of systems engineering process for the development of the system based on appropriate concepts and requirements. General operation concept and stakeholder's requirements of UHS Maglev train system must be elicited with system concept for initiating the project. The management plan should be devised for all sorts of systems engineering activities of risk management, performance management, lifecycle cost management, etc. This paper would support to establish the systems engineering management plan (SEMP) for the program of UHS Maglev train system development with associated documents.