废塑料油在船用柴油机上的燃烧特性及适用性研究

魏海军,(Wei haijun),关,德林(Guan delin),王宏志(Wang hongzhi) 한국항해항만학회 2000 한국항해항만학회 학술대회논문집 Vol.5 No.2

This paper illustrates a new test and result of WPD oil applied to marine diesel engines. In recent years, we must make an effort to develop an advanced technique for recycling waste plastics in order to utilize scrapped plastics as fuel for diesel engine. It is very important and necessary for us to cope with the increasing calorific value and the growing need of environment protection. The experiment fuel oil was obtained by mixing of diesel oil, WPD and water.

녹두의 Vitexin이 비만전구세포에서 세포분화 및 아디포사이토카인 분비능에 미치는 영향

위해리(Haeri Wi),최문지(Munji Choi),최세림(Selim Choi),김애정(Ae-Jung Kim),이명숙(Myoungsook Lee) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.8

Obesity is an important issue worldwide as it may associated with increased prevalence of metabolic diseases. Mung bean is known as a functional food for decreasing the glycemic index and lipid profile of plasma. The purpose of this study was to investigate the anti-obesity effects of vitexin from mung bean on the regulation of adipocyte differentiation and adipocytokine secretion. When 3T3-L1 adipocytes were treated with vitexin from days 0 to 14 at various levels of 25, 50, 100, and 200 μM, there was no change in cell viability. Vitexin treatment at 50, 100, and 200 μM decreased triacylglycerol levels in cells, but only 100 μM vitexin induced lipolysis. At 200 μM of vitexin, phosphorylation of p38 and ERK, which causes secretion of inflammatory adipocytokines, was depressed, whereas there was an increase in expression of PPARγ, the key regulator of adipocyte differentiation. Phosphorylation of AMPK increased at 100 μM vitexin. TNF-α and aP2 mRNA expression increased at 25 μM vitexin, whereas only TNF-α mRNA expression increased at 200 μM vitexin. Further, the mRNA levels of TNF-α and aP2 decreased at other concentrations in a dose-dependent manner. Since we observed that mRNA expression of C/EBP, SREBP1, and PPARγ did not change upon vitexin treatment, our future studies will investigate other genes such as mTOR, which is related with apoptosis signaling, or SIRT1, which is associated with inhibition of adipogenesis. Our results indicate that vitexin at concentrations between 100 and 200 μM is suitable in vivo for the development of mung bean as an anti-obesity therapy or functional food.

연구 및 세미나: 소비자의 안전하고 올바른 식생활을 위한 견과류 이야기

식품의약품안전청 위해예방정책국 한국식품산업협회 2011 좋은식품 Vol.223 No.-

Induction of Apoptosis in Cervical Carcinoma HeLa Cells by Petasites japonicus Ethanol Extracts

황유진,위해리,김행란,박계원,황경아 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

Petasites japonicus is a traditional medicine in Korea for treating carcinoma, bronchitis, and asthma. However, the molecular mechanisms of anti-cancer activity are not well understood. The purpose of this study was to confirm the anti-cancer activity of P. japonicus and its mechanism. Apoptosis was examined using morphological observation of nuclear changes and flow cytometry. Activation of apoptosis-related markers was detected. P. japonicus extract significantly inhibited HeLa cell growth in vitro in a dose-dependent manner. Induction of apoptosis by P. japonicus was mediated by up-regulation of Bax and down-regulation of Bcl-2. P. japonicus also activated caspase-9, capspase-3, and PARP, indicating induction of the mitochondrial intrinsic apoptosis pathway. We also confirmed that administration of P. japonicus significantly reduced tumor weight in the xenografted mice. Thus, P. japonicus was effective for inhibiting tumor growth in vitro and in vivo by inducing apoptosis, suggesting its usefulness as a potential anti-cancer agent.

사용 편의성을 위한 태블릿 PC용 디지털 매거진 네비게이션 디자인에 대한 연구

노승관(No, Seungkwan),위해속(Wei, Haisu) 한국디자인지식학회 2011 디자인지식저널 Vol.19 No.-

태블릿 PC의 새로운 조작 방식과 다양한 인터랙션 기능은 디지털 매거진의 디자인에 자유로운 표현 공간을 주는 반면은 기준 양식이 없어 구독자에게 인지적인 어려움을 주고 있다. 따라서 디지털 매거진간의 호환성에 대한 연구가 필요하다. 본 연구는 태블릿 PC용 매거진을 중심으로 한 디지털 매거진 네비게이션 디자인 방안을 제시함으로써 디지털 매거진의 사용 편의성과 효율성을 향상시킬 수 있는 네비게이션 정보 디자인 방식을 제안하고자 한다. 본 연구에서는 태블릿 PC용 디지털 매거진을 신문형, 간행물형, 개인화 컨텐츠형 3가지로 분류하고 애플 iPad와 삼성 갤럭시탭을 이용하여 미국, 중국, 한국의 사례별로 사례 조사를 실시하여 분석한 다음에 네비게이션 디자인 요소를 도출했다. 도출한 디지털 매거진 네비게이션 요소를 바탕으로 사용자 조사를 실시했다. 설문 조사 결과 분석을 통해 사용 편의성을 위한 태블릿 PC용 매거진 네비게이션 디자인 제안을 제시하였다. 최적화된 형태로 신문형과 간행물형의 태블릿 PC용 매거진 네비게이션 디자인 방안을 도출했다. 본 연구를 통해 얻은 결과는 향후 태블릿 PC용 매거진 디자인의 발전에 기초자료로 활용될 것으로 기대할 수 있다. Tablet PC’s touch operation and a variety of interactive features are particularly suitable for reading electronic magazines and design. But the problem has arrived. Each magazine has its own uniquestructure and mode of operation, which gives the user created a lot of trouble. E-magazines need an operating standard. This paper present a study of design of electronic magazine navigation. In this study, digital magazines for tablet PCs are divided into three categories; newspaper type, periodical type and personalized contents type. The paper used Apple iPad and Samsung Galaxy Tab to analyze and compare electronic magazine applications from three countries; United States, China and South Korea. Based on analysis of the results from comparing applications and user survey, the paper proposed navigation design proposals based on usability and an optimized navigation design models for news and periodicals were proposed. This research could be useful for base data for the development of electronic magazines.

인터넷 커뮤니케이션 환경에서 이모티콘 시각 표현에 대한 연구

노승관 ( Seung Kwan No ),위해속 ( Hai Su Wei ) 한국디지털영상학회 2010 디지털영상학술지 Vol.7 No.2

Nowadays Internet and communications technology has become very prosperous. Emoticons convey informations as a visual language. Infact they have crossed the borders or we can say that beyond the boundaries of age, recognized by many people. Particularly in the instant messenger they have a high frequency of usage, because of the large number of users they have a huge opportunity. Now emoticons are from the Internet to market. In such an environment, it is necessary to conduct a deeper research and analysis on emoticons. This paper is based on the research of emoticon impression management. The emoticons are developed by the observation and analysis of the image building process. From the perspective of visual performance, the emoticons are divided into three parts. They are the shape and the delivery of information and performance of information. For comparison, the shape is divided into two parts of shape and color. The delivery of information is divided into two parts of vehicle and process. The performance of information is divided into two parts of diversity and dilatancy. Then compare the first generation, second generation, third generation of the emoticons. Through these three parts the performance of elements of visual emoticons comparison has drawn three emoticons law of development of visual expression. First, emoticons from the abstract symbols, and gradually becomes animated characters. Second, emoticons used more body language, visual language and Information communication which means is constantly expanding. Third, the performance of the contents of the emoticons have become richer. All this shows that emoticons contains more emotional direction. With the personal web2.0 era, emoticons have become more personalized. From the perspective of impression management, self-image of people will have different requirements. Later, in order to fully develop the value of emoticons, there will be need to study the performance and methods of personality problems.

산화스트레스가 유도된 인체 간암세포 (HepG2)에서 Sulforaphane과 Diallyl Sulfide, Capsaicin, Gingerol의 항산화효과 비교연구

이소연(Lee Soyoun),위해리(Wi,Haeri),이명숙(Lee Myoungsook) 韓國營養學會 2011 Journal of Nutrition and Health Vol.44 No.6

Oxygen is necessary to sustain life, yet cellular oxygen metabolism creates destructive elements called free radicals. Free radicals are chemically unbalanced and carrying free electrons that can damage molecules, potentially damaging the cell itself. For this reason, many antioxidant products, including supplements and functional foods, are being developed. In particular, natural products are rich sources of pharmacologically active compounds. The purpose of this study was to investigate the antioxidant effects of target biomaterials in Korean traditional spices such as diallyl sulfide (DAS), capsaicin (CAP), and gingerol (GGR), and to investigate the response of the antioxidant defense system to oxidative stress by hydrogen peroxide (H2O2) compared to sulforaphane (SFN) in HepG2 cells. After the analysis of the cell viability using Cell Counting kit-8 (CCK-8) assay, we determined that the optimum levels were 200 μM DAS, 25 μM CAP, 50 μM GGR, and 12.5 μM SFN. Antioxidant enzymes were measured and protein expression was detected by Western blotting. All treatments showed a significant decrease in antioxidant enzyme activity such as superoxide dismutase, catalse, and glutathione peroxidase in HepG2 cells. Additionally, DAS, CAP, GGR and SFN increased the antioxidant system-related transcription factor Nrf2 which was found to be regulated by the activation of MAPK-JNK in this study. In conclusion, these results indicate the protective effects of DAS CAP, GGR, and SFN against H2O2-induced oxidative stress. (Korean J Nutr 2011; 44(6): 488~497)

Effects of Mung Bean (Vigna radiata L.) Ethanol Extracts Decrease Proinflammatory Cytokine-Induced Lipogenesis in the KK-Ay Diabese Mouse Model

강인혜,하태정,최문지,위해리,이병원,이명숙,최서진 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.8

Rapid increase in the prevalence of obesity-related metabolic inflammatory diseases has led to research focused on nutraceuticals for their treatment. This study investigated the effects of the ethanol extracts of mung bean testa (MBT) on the metabolic inflammation-induced lipogenesis in gastrocnemius muscle of KK-Ay diabese mice. Ethanol extracts of MBT were orally administered to diabese KK-Ay mice for 4 weeks after diet-induced obesity model was generated by feeding a 60% high-fat diet for 3 weeks. Although there were no changes in body weight gain, MBT treatments decreased total weight of white adipose tissue. MBT also decreased triacylglycerol and total cholesterol levels in the muscle by 30%, which was correlated with suppression of lipogenic genes such as ACC, C/EBP alpha, PGC-1 alpha, and PPAR gamma. In particular, decreased levels of p-ERK1/2, PPAR gamma, and C/EBP alpha in the MBT-treated groups suggest that MBT might inhibit adipogenesis and decrease differentiation via the MEK/ERK pathway. Furthermore, significantly lower amounts of plasma interleukin (IL)-6 and intramuscular tumor necrosis factor (TNF)-alpha and monocyte chemoattractant protein-1 (MCP-1) were detected in MBT groups, confirming the anti-inflammatory effect of mung bean. In addition, our in vitro pilot study with 3T3-L1 cells showed that vitexin, the functional chemical in MBT, inhibited inflammation-induced lipogenesis with significantly lower amounts of IL-6 and MCP-1 after 14 days of vitexin treatment. Thus, the functional compounds in the mung bean ethanol extracts such as vitexin and isovitexin may regulate intracellular lipogenesis and adipogenesis via antiinflammatory mechanisms and MEK/ERK pathway in the KK-Ay mouse model.

고지방 식이를 섭취한 흰쥐에서 마테의(Ilex praguariensis) 중성지방과 콜레스테롤 저하 효과

박지연,안희정,정주현,변정은,이해진,장원원,위해리,최세림,안진표,이명숙 誠信女子大學校 生活文化硏究所 2012 生活文化硏究 Vol.26 No.2

With obesity caused by high fat intake having various adverse effects on people of today's society, the aim of this study is to examine the lowering effect of proportional mate intake on tissue triglyceride and cholesterol levels. Mate, binomial name Ilex paraguariensis, is a species of holly (family Aquifoliaceae), well-known as the source of the mate beverage, which is native to subtropical South America. Five-week-old male Sprague-Dawleys weighing about 200g were used for this experiment. These rats were fed on a diet mixed with 60% fat and instant Mate powder in market circulation. They were divided into four groups, with the control group on a normal diet (n=6), a group on a high fat diet (HFD) (n=6), a group on a high fat diet containing 2% mate powder (MT2) (n=6), and the last group on a high fat diet containing 4% mate powder (MT4) (n=6). The food efficiency ratio made no difference in the MT2 group compared to the HFD group. With the intake of mate powder, serum and tissue triglycerides were decreased in the MT2 group. Especially notable was the fall of triglyceride levels in the liver in the MT2 group by roughly 60%, compared to those of the HFD group. Cholesterol levels in epididymal fat pads were sig-nificantly decreased - by 60% - in the MT4 group compared to those of the HFD group. Also, as a result of DPPH(1,1-Diphenyl-2-picrylhydrazyl) assay, the anti-oxidant activity of Mate powder showed a similar level to that of vitamin E. These findings indicate that triglyceride and cholesterol levels in liver and epididymal fat pads are decreased by the intake of Mate powder. That the dose of Mate powder in the MT4 group had a signifiant effect means that Mate may be capable of interfering in triglyceride and cholesterol levels.

뽕잎(leaf of Morus alba)분말의 섭취가 고지방 식이를 섭취한 흰 쥐의 지질저하 및 항산화 효과에 미치는 영향

유승혜,안성희,복민아,김은정,장연,위해리,최세림,안진표,이명숙 誠信女子大學校 生活文化硏究所 2012 生活文化硏究 Vol.26 No.2

People are increasingly becoming interested in the power of phytochemicals to decrease the risk of cardiovascular diseases, because cardiovascular diseases are one of the leading causes of death. Morus alba leaves containing dietary fiber and rutin are said to lower cholesterol and triglyceride levels and to have antioxidant effects. This study investigates the effects of Morus alba leaves on lipid metabolism and antioxidant functions. Male Sprague-Dawley rats were divided into four groups randomly and put on the following diets for six weeks: normal (control), high fat (HF), high fat with 10% Morus alba leaves (B10), high fat with 15% Morus alba leaves (B15). Food efficiency ratio (FER) and weight gain were not decreased. However, food intakes were significantly increased (p=0.000). Total serum cholesterol in the B15 group was reduced by 5%, a higher percentage than that of the HF group and serum triglyceride in the B10 group was reduced by 53%, a higher percentage than that of the HF group. Liver cholesterol in the B15 group was reduced by 40%, a higher percentage than that of the HF group. Liver cholesterol levels in the B10 and B15 groups were reduced by 21% and 54% respectively, higher percentages than those of the HF group. In addition, the total cholesterol level of epididymal fat pads in the B15 and B10 groups was reduced by 44%, 57% lower than that of the HF group. The triglyceride level in the B10 group was decreased by 63% more than that of the HF group. ALT and AST activities, indicators of liver damage, were not increased. The SOD level did not change but TBARS differed between groups. These results indicate that Morus alba leaves have significant effects on the lipid metabolism of serum, liver and epididymis.